Reviewed, UniProtKB/Swiss-Prot P97714 (ADA1D_MOUSE)

Names and origin

Protein names

Recommended name:
    Alpha-1D adrenergic receptor
Alternative name(s):
    Alpha 1D-adrenoreceptor
    Alpha 1D-adrenoceptor
    Alpha-1A adrenergic receptor

Gene names

Name:	Adra1d
Synonyms:	Adra1a, Gpcr8

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of the force of heart contraction during baroreceptor response to increased systemic arterial blood pressure Inferred from mutant phenotype. Source: MGI norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure Inferred from mutant phenotype. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha1-adrenergic receptor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Alpha-1D adrenergic receptor		PRO_0000069074
Regions
	Topological domain	1 – 90	90	Extracellular By similarity
	Transmembrane	91 – 115	25	1 By similarity
	Topological domain	116 – 127	12	Cytoplasmic By similarity
	Transmembrane	128 – 153	26	2 By similarity
	Topological domain	154 – 163	10	Extracellular By similarity
	Transmembrane	164 – 186	23	3 By similarity
	Topological domain	187 – 207	21	Cytoplasmic By similarity
	Transmembrane	208 – 232	25	4 By similarity
	Topological domain	233 – 245	13	Extracellular By similarity
	Transmembrane	246 – 269	24	5 By similarity
	Topological domain	270 – 342	73	Cytoplasmic By similarity
	Transmembrane	343 – 367	25	6 By similarity
	Topological domain	368 – 374	7	Extracellular By similarity
	Transmembrane	375 – 399	25	7 By similarity
	Topological domain	400 – 562	163	Cytoplasmic By similarity
	Compositional bias	21 – 57	37	Gly-rich
	Compositional bias	416 – 421	6	Poly-Arg
Amino acid modifications
	Lipidation	413	1	S-palmitoyl cysteine Potential
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Glycosylation	76	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	330	1	R → G in AAA16854. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P97714-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 6CF15515B5F7FA47	FASTA	562	59,858
	10 20 30 40 50 60 MTFRDILSVT FEGPRASSST GGSGAGGGAG TVGPEGPAVG GVPGATGGSA VVGTGSGEDN 70 80 90 100 110 120 QSSTAEAGAA ASGEVNGSAA VGGLVVSAQG VGVGVFLAAF ILTAVAGNLL VILSVACNRH 130 140 150 160 170 180 LQTVTNYFIV NLAVADLLLS AAVLPFSATM EVLGFWPFGR TFCDVWAAVD VLCCTASILS 190 200 210 220 230 240 LCTISVDRYV GVRHSLKYPA IMTERKAAAI LALLWAVALV VSVGPLLGWK EPVPPDERFC 250 260 270 280 290 300 GITEEVGYAI FSSVCSFYLP MAVIVVMYCR VYVVARSTTR SLEAGIKREP GKASEVVLRI 310 320 330 340 350 360 HCRGAATSAK GNPGTQSSKG HTLRSSLSVR LLKFSREKKA AKTLAIVVGV FVLCWFPFFF 370 380 390 400 410 420 VLPLGSLFPQ LKPSEGVFKV IFWLGYFNSC VNPLIYPCSS REFKRAFLRL LRCQCRRRRR 430 440 450 460 470 480 RLWPSLRPPL ASLDRRPALR LCPQPAHRTP RGSPSPHCTP RPGLRRHAGG AGFGLRPSKA 490 500 510 520 530 540 SLRLREWRLL GPLQRPTTQL RAKVSSLSHK FRSGGARRAE TACALRSEVE AVSLNVPQDG 550 560 AEAVICQAYE PGDLSNLRET DI

References

[1]	"Molecular cloning of alpha 1d-adrenergic receptor and tissue distribution of three alpha 1-adrenergic receptor subtypes in mouse." Alonso-Llamazares A., Zamanillo D., Casanova E., Ovalle S., Calvo P., Chinchetru M.A. J. Neurochem. 65:2387-2392(1995) [PubMed: 7595531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Characterization of the mouse alpha1D-adrenergic receptor gene." Arai K., Tanoue A., Goda N., Takeda M., Takahashi K., Tsujimoto G. Jpn. J. Pharmacol. 81:271-278(1999) [PubMed: 10622215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Identification, chromosomal location, and genome organization of mammalian G-protein-coupled receptors." Wilkie T.M., Chen Y., Gilbert D.J., Moore K.J., Yu L., Simon M.I., Copeland N.G., Jenkins N.A. Genomics 18:175-184(1993) [PubMed: 8288218] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 190-350. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S80044 mRNA. Translation: AAB47042.1. AB030642 Genomic DNA. Translation: BAA90312.1. L20333 mRNA. Translation: AAA16854.1.
IPI	IPI00128833.
PIR	D48909.
UniGene	Mm.389380
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	P97714.
Genome annotation databases
Ensembl	ENSMUSG00000027335. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:106673. Adra1d.
Phylogenomic databases
HOGENOM	P97714.
HOVERGEN	P97714.
Gene expression databases
ArrayExpress	P97714.
CleanEx	MM_ADRA1A. MM_ADRA1D.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR000363. Adren_rcpt_A1A. IPR002233. Adrnrgc_rcpt. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01103. ADRENERGICR. PR00240. ADRENRGCA1DR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

ADA1D_MOUSE

Accession

Primary (citable) accession number: P97714
Secondary accession number(s): Q61619

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	July 7, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)