Reviewed, UniProtKB/Swiss-Prot P97711 (GRK6_RAT)

Names and origin

Protein names

Recommended name:
    G protein-coupled receptor kinase 6
    EC=2.7.11.16
Alternative name(s):
    G protein-coupled receptor kinase GRK6

Gene names

Name:	Grk6
Synonyms:	Gprk6

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically phosphorylates the activated forms of G protein-coupled receptors By similarity.
Catalytic activity	ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
Subcellular location	Membrane; Lipid-anchor.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from direct assay. Source: RGD signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	G protein-coupled receptor kinase 6		PRO_0000085976
Regions
	Domain	53 – 171	119	RGS
	Domain	186 – 448	263	Protein kinase
	Domain	449 – 514	66	AGC-kinase C-terminal
	Nucleotide binding	192 – 200	9	ATP By similarity
	Region	1 – 185	185	N-terminal
Sites
	Active site	311	1	Proton acceptor By similarity
	Binding site	215	1	ATP By similarity
Amino acid modifications
	Modified residue	484	1	Phosphoserine; by autocatalysis By similarity
	Modified residue	485	1	Phosphothreonine; by autocatalysis By similarity
	Lipidation	561	1	S-palmitoyl cysteine By similarity
	Lipidation	562	1	S-palmitoyl cysteine By similarity
	Lipidation	565	1	S-palmitoyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97711-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 0089AC9EE231F5C9	FASTA	576	65,963
	10 20 30 40 50 60 MELENIVANT VLLKAREGGG GNRKGKSKKW RQMLQFPHIS QCEELRLSLE RDYHSLCERQ 70 80 90 100 110 120 PIGRLLFREF CATRPELTRC TAFLDGVAEY EVTPDEKRKA CGCRLMQNFL SHTGPDLIPE 130 140 150 160 170 180 VPRQLVSNCA QRLEQGPCKD LFQELTRLTH EYLSMAPFAD YLDSIYFNRF LQWKWLERQP 190 200 210 220 230 240 VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKQILEK 250 260 270 280 290 300 VNSRFVVSLA YAYETKDALC LVLTLMNGGD LKFHIYHMGQ AGFPEARAVF YAAEICCGLE 310 320 330 340 350 360 DLHRERIVYR DLKPENILLD DHGHIRISDL GLTVHVPEGQ TIKGRVGTVG YMAPEVVKNE 370 380 390 400 410 420 RYTFSPDWWA LGCLLYEMIA GQSPFQQRKK KIKREEVERL VKEVAEEYTD RFSPQARSLC 430 440 450 460 470 480 SQLPNKDPAE RLGCRGGGAR EVKEHPLFKK LNFKRLGAGM LEPPFKPDPQ AIYCKDVLDI 490 500 510 520 530 540 EQFSTVKGVD LEPTDQDFYQ KFATGSVSIP WQNEMVETEC FQELNVFGLD GSVPPDLDWK 550 560 570 GQPTAPPKKG LLQRLFSRQD CCGNCSDSEE ELPTRL

References

[1]	"Molecular cloning of rat G-protein-coupled receptor kinase 6 (GRK6) from brain tissue, and its mRNA expression in different brain regions and peripheral tissues." Fehr C., Fickova M., Hiemke C., Reuss S., Dahmen N. Brain Res. Mol. Brain Res. 49:278-282(1997) [PubMed: 9387888] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09365 mRNA. Translation: CAA70542.1.
IPI	IPI00205494.
UniGene	Rn.10633
3D structure databases
SMR	P97711. Positions 23-532.
ModBase	Search...
Protein-protein interaction databases
STRING	P97711.
PTM databases
PhosphoSite	P97711.
Genome annotation databases
Ensembl	ENSRNOT00000020214; ENSRNOP00000020214; ENSRNOG00000014615; Rattus norvegicus. [Genome view] ENSRNOT00000047958; ENSRNOP00000050134; ENSRNOG00000014615; Rattus norvegicus. [Genome view]
UCSC	NM_031657. rat.
Organism-specific databases
RGD	61986. Gprk6.
Phylogenomic databases
HOVERGEN	P97711.
Enzyme and pathway databases
BRENDA	2.7.11.16. 248.
Gene expression databases
ArrayExpress	P97711.
Genevestigator	P97711.
GermOnline	ENSRNOG00000014615. Rattus norvegicus.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRK6_RAT

Accession

Primary (citable) accession number: P97711

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	November 24, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)