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P97711 (GRK6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G protein-coupled receptor kinase 6

EC=2.7.11.16
Alternative name(s):
G protein-coupled receptor kinase GRK6
Gene names
Name:Grk6
Synonyms:Gprk6
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis By similarity. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro) By similarity.

Catalytic activity

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Subcellular location

Membrane; Lipid-anchor.

Tissue specificity

Widely expressed. Detectable in all brain areas examined. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Contains 1 RGS domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576G protein-coupled receptor kinase 6
PRO_0000085976

Regions

Domain53 – 171119RGS
Domain186 – 448263Protein kinase
Domain449 – 51466AGC-kinase C-terminal
Nucleotide binding192 – 2009ATP By similarity
Nucleotide binding264 – 2707ATP By similarity
Nucleotide binding315 – 3184ATP By similarity
Region1 – 185185N-terminal

Sites

Active site3111Proton acceptor By similarity
Binding site2151ATP By similarity

Amino acid modifications

Modified residue4841Phosphoserine; by autocatalysis By similarity
Modified residue4851Phosphothreonine; by autocatalysis By similarity
Lipidation5611S-palmitoyl cysteine By similarity
Lipidation5621S-palmitoyl cysteine By similarity
Lipidation5651S-palmitoyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
P97711 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 0089AC9EE231F5C9

FASTA57665,963
        10         20         30         40         50         60 
MELENIVANT VLLKAREGGG GNRKGKSKKW RQMLQFPHIS QCEELRLSLE RDYHSLCERQ 

        70         80         90        100        110        120 
PIGRLLFREF CATRPELTRC TAFLDGVAEY EVTPDEKRKA CGCRLMQNFL SHTGPDLIPE 

       130        140        150        160        170        180 
VPRQLVSNCA QRLEQGPCKD LFQELTRLTH EYLSMAPFAD YLDSIYFNRF LQWKWLERQP 

       190        200        210        220        230        240 
VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKQILEK 

       250        260        270        280        290        300 
VNSRFVVSLA YAYETKDALC LVLTLMNGGD LKFHIYHMGQ AGFPEARAVF YAAEICCGLE 

       310        320        330        340        350        360 
DLHRERIVYR DLKPENILLD DHGHIRISDL GLTVHVPEGQ TIKGRVGTVG YMAPEVVKNE 

       370        380        390        400        410        420 
RYTFSPDWWA LGCLLYEMIA GQSPFQQRKK KIKREEVERL VKEVAEEYTD RFSPQARSLC 

       430        440        450        460        470        480 
SQLPNKDPAE RLGCRGGGAR EVKEHPLFKK LNFKRLGAGM LEPPFKPDPQ AIYCKDVLDI 

       490        500        510        520        530        540 
EQFSTVKGVD LEPTDQDFYQ KFATGSVSIP WQNEMVETEC FQELNVFGLD GSVPPDLDWK 

       550        560        570 
GQPTAPPKKG LLQRLFSRQD CCGNCSDSEE ELPTRL 

« Hide

References

[1]"Molecular cloning of rat G-protein-coupled receptor kinase 6 (GRK6) from brain tissue, and its mRNA expression in different brain regions and peripheral tissues."
Fehr C., Fickova M., Hiemke C., Reuss S., Dahmen N.
Brain Res. Mol. Brain Res. 49:278-282(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09365 mRNA. Translation: CAA70542.1.
UniGeneRn.10633.

3D structure databases

ProteinModelPortalP97711.
SMRP97711. Positions 23-532.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP97711.

Proteomic databases

PaxDbP97711.
PRIDEP97711.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:61986. rat.

Organism-specific databases

RGD61986. Grk6.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000006742.
HOVERGENHBG004532.
PhylomeDBP97711.

Enzyme and pathway databases

BRENDA2.7.11.16. 5301.

Gene expression databases

GenevestigatorP97711.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR00717. GPCRKINASE.
SMARTSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGRK6_RAT
AccessionPrimary (citable) accession number: P97711
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families