G protein-coupled receptor kinase 6 - Rattus norvegicus (Rat)

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P97711 (GRK6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
G protein-coupled receptor kinase 6
EC=2.7.11.16

Alternative name(s):
G protein-coupled receptor kinase GRK6

Gene names

Name:	Grk6
Synonyms:	Gprk6

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis By similarity. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro) By similarity.
Catalytic activity	ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
Subcellular location	Membrane; Lipid-anchor.
Tissue specificity	Widely expressed. Detectable in all brain areas examined. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW desensitization of G-protein coupled receptor protein signaling pathway Inferred from direct assay PubMed 12810562. Source: RGD negative regulation of anion channel activity Inferred from mutant phenotype PubMed 17170521. Source: RGD termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from direct assay PubMed 12810562. Source: RGD plasma membrane Inferred from direct assay PubMed 12810562. Source: RGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from direct assay PubMed 12810562. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 576

576

G protein-coupled receptor kinase 6

PRO_0000085976

Regions

Domain

53 – 171

119

RGS

Domain

186 – 448

263

Protein kinase

Domain

449 – 514

AGC-kinase C-terminal

Nucleotide binding

192 – 200

ATP By similarity

Nucleotide binding

264 – 270

ATP By similarity

Nucleotide binding

315 – 318

ATP By similarity

Region

1 – 185

185

N-terminal

Sites

Active site

311

Proton acceptor By similarity

Binding site

215

ATP By similarity

Amino acid modifications

Modified residue

484

Phosphoserine; by autocatalysis By similarity

Modified residue

485

Phosphothreonine; by autocatalysis By similarity

Lipidation

561

S-palmitoyl cysteine By similarity

Lipidation

562

S-palmitoyl cysteine By similarity

Lipidation

565

S-palmitoyl cysteine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P97711 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 0089AC9EE231F5C9
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FASTA

576

65,963

        10         20         30         40         50         60 
MELENIVANT VLLKAREGGG GNRKGKSKKW RQMLQFPHIS QCEELRLSLE RDYHSLCERQ 

        70         80         90        100        110        120 
PIGRLLFREF CATRPELTRC TAFLDGVAEY EVTPDEKRKA CGCRLMQNFL SHTGPDLIPE 

       130        140        150        160        170        180 
VPRQLVSNCA QRLEQGPCKD LFQELTRLTH EYLSMAPFAD YLDSIYFNRF LQWKWLERQP 

       190        200        210        220        230        240 
VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKQILEK 

       250        260        270        280        290        300 
VNSRFVVSLA YAYETKDALC LVLTLMNGGD LKFHIYHMGQ AGFPEARAVF YAAEICCGLE 

       310        320        330        340        350        360 
DLHRERIVYR DLKPENILLD DHGHIRISDL GLTVHVPEGQ TIKGRVGTVG YMAPEVVKNE 

       370        380        390        400        410        420 
RYTFSPDWWA LGCLLYEMIA GQSPFQQRKK KIKREEVERL VKEVAEEYTD RFSPQARSLC 

       430        440        450        460        470        480 
SQLPNKDPAE RLGCRGGGAR EVKEHPLFKK LNFKRLGAGM LEPPFKPDPQ AIYCKDVLDI 

       490        500        510        520        530        540 
EQFSTVKGVD LEPTDQDFYQ KFATGSVSIP WQNEMVETEC FQELNVFGLD GSVPPDLDWK 

       550        560        570 
GQPTAPPKKG LLQRLFSRQD CCGNCSDSEE ELPTRL

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References

[1]	"Molecular cloning of rat G-protein-coupled receptor kinase 6 (GRK6) from brain tissue, and its mRNA expression in different brain regions and peripheral tissues." Fehr C., Fickova M., Hiemke C., Reuss S., Dahmen N. Brain Res. Mol. Brain Res. 49:278-282(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09365 mRNA. Translation: CAA70542.1.
IPI	IPI00205494.
UniGene	Rn.10633.
3D structure databases
ProteinModelPortal	P97711.
SMR	P97711. Positions 23-532.
ModBase	Search...
PTM databases
PhosphoSite	P97711.
Proteomic databases
PaxDb	P97711.
PRIDE	P97711.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:61986. rat.
Organism-specific databases
RGD	61986. Grk6.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000006742.
HOVERGEN	HBG004532.
OrthoDB	EOG4HHP24.
Enzyme and pathway databases
BRENDA	2.7.11.16. 5301.
Gene expression databases
ArrayExpress	P97711.
Genevestigator	P97711.
GermOnline	ENSRNOG00000014615. Rattus norvegicus.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRK6_RAT

Accession

Primary (citable) accession number: P97711

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents