Reviewed, UniProtKB/Swiss-Prot P97698 (SMO_RAT)

Names and origin

Protein names

Recommended name:
    Smoothened homolog
      Short name=SMO

Gene names

Name:	Smo
Synonyms:	Smoh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	793 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO).
Subunit structure	Interacts with ARRB2 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	In embryo, found in the early neural folds and neural tube, pre-somitic mesoderm and somites, developing limb bud, gut, eye, testes, cartilage, muscle, lung, epiglottis, thymus, tongue, jaw, taste buds, teeth, and skin. In adult, found in multiple tissues including heart, brain, liver, lung, skeletal muscle, kidney and testis.
Sequence similarities	Belongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane
Molecular function	Developmental protein G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW facial nerve development Inferred from expression pattern. Source: RGD neuron projection regeneration Inferred from expression pattern. Source: RGD response to wounding Inferred from expression pattern. Source: RGD
Cellular component	cell soma Inferred from direct assay. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW non-G-protein coupled 7TM receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 793	763	Smoothened homolog		PRO_0000013017
Regions
	Topological domain	31 – 237	207	Extracellular Potential
	Transmembrane	238 – 258	21	1 Potential
	Topological domain	259 – 266	8	Cytoplasmic Potential
	Transmembrane	267 – 287	21	2 Potential
	Topological domain	288 – 318	31	Extracellular Potential
	Transmembrane	319 – 339	21	3 Potential
	Topological domain	340 – 362	23	Cytoplasmic Potential
	Transmembrane	363 – 383	21	4 Potential
	Topological domain	384 – 406	23	Extracellular Potential
	Transmembrane	407 – 427	21	5 Potential
	Topological domain	428 – 455	28	Cytoplasmic Potential
	Transmembrane	456 – 476	21	6 Potential
	Topological domain	477 – 528	52	Extracellular Potential
	Transmembrane	529 – 549	21	7 Potential
	Topological domain	550 – 793	244	Cytoplasmic Potential
	Domain	69 – 185	117	FZ
Amino acid modifications
	Modified residue	791	1	Phosphoserine By similarity
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Glycosylation	497	1	N-linked (GlcNAc...) Potential
	Disulfide bond	74 ↔ 138		By similarity
	Disulfide bond	82 ↔ 131		By similarity
	Disulfide bond	122 ↔ 158		By similarity
	Disulfide bond	151 ↔ 173		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97698-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 27B88178B2565FB9	FASTA	793	87,376
	10 20 30 40 50 60 MAAGRPVRGP ELAPRRLLQL LLLVLLGGRG RGAALSGNVT GPGPRSAGGS ARRNAPVTSP 70 80 90 100 110 120 PPPLLSHCGR AAHCEPLRYN VCLGSALPYG ATTTLLAGDS DSQEEAHSKL VLWSGLRNAP 130 140 150 160 170 180 RCWAVIQPLL CAVYMPKCEN DRVELPSRTL CQATRGPCAI VERERGWPDF LRCTPDHFPE 190 200 210 220 230 240 GCPNEVQNIK FNSSGQCEAP LVRTDNPKSW YEDVEGCGIQ CQNPLFTEAE HQDMHSYIAA 250 260 270 280 290 300 FGAVTGLCTL FTLATFVADW RNSNRYPAVI LFYVNACFFV GSIGWLAQFM DGARREIVCR 310 320 330 340 350 360 ADGTMRFGEP TSSETLSCVI IFVIVYYALM AGVVWFVVLT YAWHTSFKAL GTTYQPLSGK 370 380 390 400 410 420 TSYFHLLTWS LPFVLTVAIL AVAQVDGDSV SGICFVGYKN YRYRAGFVLA PIGLVLIVGG 430 440 450 460 470 480 YFLIRGVMTL FSIKSNHPGL LSEKAASKIN ETMLRLGIFG FLAFGFVLIT FSCHFYDFFN 490 500 510 520 530 540 QAEWERSFRD YVLCQANVTI GLPTKKPIPD CEIKNRPSLL VEKINLFAMF GTGIAMSTWV 550 560 570 580 590 600 WTKATLLIWR RTWCRLTGHS DDEPKRIKKS KMIAKAFSKR RELLQNPGQE LSFSMHTVSH 610 620 630 640 650 660 DGPVAGLAFE LNEPSADVSS AWAQHVTKMV ARRGAILPQD VSVTPVATPV PPEEQANLWL 670 680 690 700 710 720 VEAEISPELE KRLGRKKKRR KRKKEVCPLG PAPELHHSAP VPATSAVPRL PQLPRQKCLV 730 740 750 760 770 780 AANAWGTGEP CRQGAWTVVS NPFCPEPSPH QDPFLPGASA PRVWAQGRLQ GLGSIHSRTN 790 LMEAELLDAD SDF

References

[1]	"The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog." Stone D.M., Hynes M., Armanini M., Swanson T.A., Gu Q., Johnson R.L., Scott M.P., Pennica D., Goddard A., Phillips H., Noll M., Hooper J.E., de Sauvage F., Rosenthal A. Nature 384:129-134(1996) [PubMed: 8906787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U84402 mRNA. Translation: AAB41789.1.
IPI	IPI00206713.
RefSeq	NP_036939.1.
UniGene	Rn.44429
3D structure databases
SMR	P97698. Positions 72-181.
ModBase	Search...
Protein-protein interaction databases
STRING	P97698.
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSRNOT00000011356; ENSRNOP00000011356; ENSRNOG00000008332; Rattus norvegicus. [Genome view]
GeneID	25273.
KEGG	rno:25273.
Organism-specific databases
CTD	25273.
RGD	3726. Smo.
Phylogenomic databases
eggNOG	roNOG05761.
HOVERGEN	P97698.
InParanoid	P97698.
Gene expression databases
ArrayExpress	P97698.
Genevestigator	P97698.
GermOnline	ENSRNOG00000008332. Rattus norvegicus.
Family and domain databases
InterPro	IPR000539. Frizzled. IPR020067. Frizzled-like_dom. IPR000024. Frizzled_Cys-rich. IPR020068. Frizzled_dom_subgr. IPR015526. Frizzled_related. IPR017981. GPCR_2-like. [Graphical view]
Gene3D	G3DSA:1.10.2000.10. Frizzled_Cys-rich. 1 hit.
PANTHER	PTHR11309. Fz_related. 1 hit.
Pfam	PF01534. Frizzled. 1 hit. PF01392. Fz. 1 hit. [Graphical view]
PRINTS	PR00489. FRIZZLED.
SMART	SM00063. FRI. 1 hit. [Graphical view]
PROSITE	PS50038. FZ. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	605965.

Entry information

Entry name

SMO_RAT

Accession

Primary (citable) accession number: P97698

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	January 19, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)