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Protein

Smoothened homolog

Gene

Smo

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia (By similarity).By similarity

GO - Molecular functioni

  1. drug binding Source: UniProtKB
  2. G-protein coupled receptor activity Source: GO_Central
  3. patched binding Source: GO_Central
  4. Wnt-protein binding Source: GO_Central

GO - Biological processi

  1. axon extension involved in axon guidance Source: RGD
  2. determination of left/right asymmetry in lateral mesoderm Source: UniProtKB
  3. facial nerve development Source: RGD
  4. forebrain morphogenesis Source: UniProtKB
  5. heart looping Source: UniProtKB
  6. mesenchymal to epithelial transition involved in metanephric renal vesicle formation Source: UniProtKB
  7. midgut development Source: UniProtKB
  8. negative regulation of DNA binding Source: UniProtKB
  9. negative regulation of gene expression Source: UniProtKB
  10. neuron projection regeneration Source: RGD
  11. osteoblast differentiation Source: RGD
  12. osteoblast proliferation Source: RGD
  13. positive regulation of branching involved in ureteric bud morphogenesis Source: UniProtKB
  14. positive regulation of gene expression Source: UniProtKB
  15. positive regulation of hh target transcription factor activity Source: UniProtKB
  16. response to organic substance Source: RGD
  17. response to wounding Source: RGD
  18. smoothened signaling pathway Source: UniProtKB
  19. somite development Source: UniProtKB
  20. spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Smoothened homolog
Short name:
SMO
Gene namesi
Name:Smo
Synonyms:Smoh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3726. Smo.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini31 – 237207ExtracellularSequence AnalysisAdd
BLAST
Transmembranei238 – 25821Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini259 – 2668CytoplasmicSequence Analysis
Transmembranei267 – 28721Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini288 – 31831ExtracellularSequence AnalysisAdd
BLAST
Transmembranei319 – 33921Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini340 – 36223CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei363 – 38321Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini384 – 40623ExtracellularSequence AnalysisAdd
BLAST
Transmembranei407 – 42721Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini428 – 45528CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei456 – 47621Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini477 – 52852ExtracellularSequence AnalysisAdd
BLAST
Transmembranei529 – 54921Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini550 – 793244CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. axonal growth cone Source: RGD
  2. cilium Source: UniProtKB-SubCell
  3. cytoplasm Source: RGD
  4. dendrite Source: RGD
  5. dendritic growth cone Source: RGD
  6. integral component of membrane Source: GO_Central
  7. neuronal cell body Source: RGD
  8. plasma membrane Source: UniProtKB
  9. postsynaptic density Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 793763Smoothened homologPRO_0000013017Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi74 ↔ 138PROSITE-ProRule annotation
Disulfide bondi82 ↔ 131PROSITE-ProRule annotation
Disulfide bondi122 ↔ 158PROSITE-ProRule annotation
Disulfide bondi151 ↔ 173PROSITE-ProRule annotation
Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi197 ↔ 217PROSITE-ProRule annotation
Disulfide bondi221 ↔ 299PROSITE-ProRule annotation
Disulfide bondi318 ↔ 394PROSITE-ProRule annotation
Disulfide bondi494 ↔ 511PROSITE-ProRule annotation
Glycosylationi497 – 4971N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP97698.
PRIDEiP97698.

PTM databases

PhosphoSiteiP97698.

Expressioni

Tissue specificityi

In embryo, found in the early neural folds and neural tube, pre-somitic mesoderm and somites, developing limb bud, gut, eye, testes, cartilage, muscle, lung, epiglottis, thymus, tongue, jaw, taste buds, teeth, and skin. In adult, found in multiple tissues including heart, brain, liver, lung, skeletal muscle, kidney and testis.

Gene expression databases

GenevestigatoriP97698.

Interactioni

Subunit structurei

Homodimer. Interacts with ARRB2. Interacts with KIF7. Interacts with BBS5 and BBS7; the interactions are indicative for the association of SMO with the BBsome complex to facilitate ciliary localization of SMO (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011356.

Structurei

3D structure databases

ProteinModelPortaliP97698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 185117FZPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni542 – 57332Interaction with BBS5 and BBS7By similarityAdd
BLAST

Sequence similaritiesi

Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG312505.
HOGENOMiHOG000252964.
HOVERGENiHBG000352.
InParanoidiP97698.
KOiK06226.
PhylomeDBiP97698.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR017981. GPCR_2-like.
IPR026544. SMO.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF35. PTHR11309:SF35. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97698-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAGRPVRGP ELAPRRLLQL LLLVLLGGRG RGAALSGNVT GPGPRSAGGS
60 70 80 90 100
ARRNAPVTSP PPPLLSHCGR AAHCEPLRYN VCLGSALPYG ATTTLLAGDS
110 120 130 140 150
DSQEEAHSKL VLWSGLRNAP RCWAVIQPLL CAVYMPKCEN DRVELPSRTL
160 170 180 190 200
CQATRGPCAI VERERGWPDF LRCTPDHFPE GCPNEVQNIK FNSSGQCEAP
210 220 230 240 250
LVRTDNPKSW YEDVEGCGIQ CQNPLFTEAE HQDMHSYIAA FGAVTGLCTL
260 270 280 290 300
FTLATFVADW RNSNRYPAVI LFYVNACFFV GSIGWLAQFM DGARREIVCR
310 320 330 340 350
ADGTMRFGEP TSSETLSCVI IFVIVYYALM AGVVWFVVLT YAWHTSFKAL
360 370 380 390 400
GTTYQPLSGK TSYFHLLTWS LPFVLTVAIL AVAQVDGDSV SGICFVGYKN
410 420 430 440 450
YRYRAGFVLA PIGLVLIVGG YFLIRGVMTL FSIKSNHPGL LSEKAASKIN
460 470 480 490 500
ETMLRLGIFG FLAFGFVLIT FSCHFYDFFN QAEWERSFRD YVLCQANVTI
510 520 530 540 550
GLPTKKPIPD CEIKNRPSLL VEKINLFAMF GTGIAMSTWV WTKATLLIWR
560 570 580 590 600
RTWCRLTGHS DDEPKRIKKS KMIAKAFSKR RELLQNPGQE LSFSMHTVSH
610 620 630 640 650
DGPVAGLAFE LNEPSADVSS AWAQHVTKMV ARRGAILPQD VSVTPVATPV
660 670 680 690 700
PPEEQANLWL VEAEISPELE KRLGRKKKRR KRKKEVCPLG PAPELHHSAP
710 720 730 740 750
VPATSAVPRL PQLPRQKCLV AANAWGTGEP CRQGAWTVVS NPFCPEPSPH
760 770 780 790
QDPFLPGASA PRVWAQGRLQ GLGSIHSRTN LMEAELLDAD SDF
Length:793
Mass (Da):87,376
Last modified:May 1, 1997 - v1
Checksum:i27B88178B2565FB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84402 mRNA. Translation: AAB41789.1.
RefSeqiNP_036939.1. NM_012807.1.
UniGeneiRn.44429.

Genome annotation databases

GeneIDi25273.
KEGGirno:25273.
UCSCiRGD:3726. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84402 mRNA. Translation: AAB41789.1.
RefSeqiNP_036939.1. NM_012807.1.
UniGeneiRn.44429.

3D structure databases

ProteinModelPortaliP97698.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011356.

Chemistry

ChEMBLiCHEMBL1795175.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP97698.

Proteomic databases

PaxDbiP97698.
PRIDEiP97698.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25273.
KEGGirno:25273.
UCSCiRGD:3726. rat.

Organism-specific databases

CTDi6608.
RGDi3726. Smo.

Phylogenomic databases

eggNOGiNOG312505.
HOGENOMiHOG000252964.
HOVERGENiHBG000352.
InParanoidiP97698.
KOiK06226.
PhylomeDBiP97698.

Miscellaneous databases

NextBioi605965.
PROiP97698.

Gene expression databases

GenevestigatoriP97698.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR017981. GPCR_2-like.
IPR026544. SMO.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF35. PTHR11309:SF35. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiSMO_RAT
AccessioniPrimary (citable) accession number: P97698
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: February 4, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.