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P97698 (SMO_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Smoothened homolog

Short name=SMO
Gene names
Name:Smo
Synonyms:Smoh
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length793 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia By similarity.

Subunit structure

Homodimer. Interacts with ARRB2. Interacts with KIF7. Interacts with BBS5 and BBS7; the interactions are indicative for the association of SMO with the BBsome complex to facilitate ciliary localization of SMO By similarity.

Subcellular location

Membrane; Multi-pass membrane protein. Cell projectioncilium By similarity.

Tissue specificity

In embryo, found in the early neural folds and neural tube, pre-somitic mesoderm and somites, developing limb bud, gut, eye, testes, cartilage, muscle, lung, epiglottis, thymus, tongue, jaw, taste buds, teeth, and skin. In adult, found in multiple tissues including heart, brain, liver, lung, skeletal muscle, kidney and testis.

Sequence similarities

Belongs to the G-protein coupled receptor Fz/Smo family.

Contains 1 FZ (frizzled) domain.

Ontologies

Keywords
   Cellular componentCell projection
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionDevelopmental protein
G-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadenohypophysis development

Inferred from Biological aspect of Ancestor. Source: RefGenome

axon extension involved in axon guidance

Inferred from mutant phenotype PubMed 19447091. Source: RGD

canonical Wnt signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

cardioblast differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

central nervous system neuron differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

cerebellar cortex morphogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

ciliary receptor clustering involved in smoothened signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

determination of left/right asymmetry in lateral mesoderm

Inferred from sequence or structural similarity. Source: UniProtKB

determination of left/right symmetry

Inferred from Biological aspect of Ancestor. Source: RefGenome

dorsal/ventral neural tube patterning

Inferred from Biological aspect of Ancestor. Source: RefGenome

embryonic camera-type eye development

Inferred from Biological aspect of Ancestor. Source: RefGenome

embryonic digestive tract morphogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

embryonic neurocranium morphogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

embryonic viscerocranium morphogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

exocrine pancreas development

Inferred from Biological aspect of Ancestor. Source: RefGenome

facial nerve development

Inferred from expression pattern PubMed 15356205. Source: RGD

floor plate formation

Inferred from Biological aspect of Ancestor. Source: RefGenome

forebrain morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

gonad development

Inferred from Biological aspect of Ancestor. Source: RefGenome

heart looping

Inferred from sequence or structural similarity. Source: UniProtKB

heart morphogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

mesenchymal to epithelial transition involved in metanephric renal vesicle formation

Inferred from sequence or structural similarity. Source: UniProtKB

midgut development

Inferred from sequence or structural similarity. Source: UniProtKB

muscle cell fate commitment

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of apoptotic process

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of gene expression

Inferred from sequence or structural similarity. Source: UniProtKB

neural crest cell migration

Inferred from Biological aspect of Ancestor. Source: RefGenome

neuron fate commitment

Inferred from Biological aspect of Ancestor. Source: RefGenome

neuron projection regeneration

Inferred from expression pattern PubMed 15356205. Source: RGD

odontogenesis of dentin-containing tooth

Inferred from Biological aspect of Ancestor. Source: RefGenome

osteoblast differentiation

Inferred from expression pattern PubMed 19649528. Source: RGD

osteoblast proliferation

Inferred from expression pattern PubMed 19649528. Source: RGD

otolith morphogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of branching involved in ureteric bud morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of epithelial cell proliferation

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of gene expression

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of hh target transcription factor activity

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of mesenchymal cell proliferation

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of neuroblast proliferation

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of smoothened signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

response to organic substance

Inferred from expression pattern PubMed 19429033. Source: RGD

response to wounding

Inferred from expression pattern PubMed 15356205. Source: RGD

semicircular canal morphogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

smoothened signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation

Inferred from Biological aspect of Ancestor. Source: RefGenome

smoothened signaling pathway involved in ventral spinal cord patterning

Inferred from Biological aspect of Ancestor. Source: RefGenome

somite development

Inferred from sequence or structural similarity. Source: UniProtKB

spermatogenesis

Inferred from expression pattern PubMed 19484749. Source: RGD

vasculogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentaxonal growth cone

Inferred from direct assay PubMed 21618238. Source: RGD

cilium

Inferred from Biological aspect of Ancestor. Source: RefGenome

cytoplasm

Inferred from direct assay PubMed 19484749. Source: RGD

dendrite

Inferred from direct assay PubMed 21618238. Source: RGD

dendritic growth cone

Inferred from direct assay PubMed 21618238. Source: RGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuronal cell body

Inferred from direct assay PubMed 15356205. Source: RGD

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

postsynaptic density

Inferred from direct assay PubMed 21618238. Source: RGD

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

PDZ domain binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-activated receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-protein binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

drug binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 793763Smoothened homolog
PRO_0000013017

Regions

Topological domain31 – 237207Extracellular Potential
Transmembrane238 – 25821Helical; Name=1; Potential
Topological domain259 – 2668Cytoplasmic Potential
Transmembrane267 – 28721Helical; Name=2; Potential
Topological domain288 – 31831Extracellular Potential
Transmembrane319 – 33921Helical; Name=3; Potential
Topological domain340 – 36223Cytoplasmic Potential
Transmembrane363 – 38321Helical; Name=4; Potential
Topological domain384 – 40623Extracellular Potential
Transmembrane407 – 42721Helical; Name=5; Potential
Topological domain428 – 45528Cytoplasmic Potential
Transmembrane456 – 47621Helical; Name=6; Potential
Topological domain477 – 52852Extracellular Potential
Transmembrane529 – 54921Helical; Name=7; Potential
Topological domain550 – 793244Cytoplasmic Potential
Domain69 – 185117FZ
Region542 – 57332Interaction with BBS5 and BBS7 By similarity

Amino acid modifications

Glycosylation381N-linked (GlcNAc...) Potential
Glycosylation1921N-linked (GlcNAc...) Potential
Glycosylation4971N-linked (GlcNAc...) Potential
Disulfide bond74 ↔ 138 By similarity
Disulfide bond82 ↔ 131 By similarity
Disulfide bond122 ↔ 158 By similarity
Disulfide bond151 ↔ 173 By similarity
Disulfide bond197 ↔ 217 By similarity
Disulfide bond221 ↔ 299 By similarity
Disulfide bond318 ↔ 394 By similarity
Disulfide bond494 ↔ 511 By similarity

Sequences

Sequence LengthMass (Da)Tools
P97698 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 27B88178B2565FB9

FASTA79387,376
        10         20         30         40         50         60 
MAAGRPVRGP ELAPRRLLQL LLLVLLGGRG RGAALSGNVT GPGPRSAGGS ARRNAPVTSP 

        70         80         90        100        110        120 
PPPLLSHCGR AAHCEPLRYN VCLGSALPYG ATTTLLAGDS DSQEEAHSKL VLWSGLRNAP 

       130        140        150        160        170        180 
RCWAVIQPLL CAVYMPKCEN DRVELPSRTL CQATRGPCAI VERERGWPDF LRCTPDHFPE 

       190        200        210        220        230        240 
GCPNEVQNIK FNSSGQCEAP LVRTDNPKSW YEDVEGCGIQ CQNPLFTEAE HQDMHSYIAA 

       250        260        270        280        290        300 
FGAVTGLCTL FTLATFVADW RNSNRYPAVI LFYVNACFFV GSIGWLAQFM DGARREIVCR 

       310        320        330        340        350        360 
ADGTMRFGEP TSSETLSCVI IFVIVYYALM AGVVWFVVLT YAWHTSFKAL GTTYQPLSGK 

       370        380        390        400        410        420 
TSYFHLLTWS LPFVLTVAIL AVAQVDGDSV SGICFVGYKN YRYRAGFVLA PIGLVLIVGG 

       430        440        450        460        470        480 
YFLIRGVMTL FSIKSNHPGL LSEKAASKIN ETMLRLGIFG FLAFGFVLIT FSCHFYDFFN 

       490        500        510        520        530        540 
QAEWERSFRD YVLCQANVTI GLPTKKPIPD CEIKNRPSLL VEKINLFAMF GTGIAMSTWV 

       550        560        570        580        590        600 
WTKATLLIWR RTWCRLTGHS DDEPKRIKKS KMIAKAFSKR RELLQNPGQE LSFSMHTVSH 

       610        620        630        640        650        660 
DGPVAGLAFE LNEPSADVSS AWAQHVTKMV ARRGAILPQD VSVTPVATPV PPEEQANLWL 

       670        680        690        700        710        720 
VEAEISPELE KRLGRKKKRR KRKKEVCPLG PAPELHHSAP VPATSAVPRL PQLPRQKCLV 

       730        740        750        760        770        780 
AANAWGTGEP CRQGAWTVVS NPFCPEPSPH QDPFLPGASA PRVWAQGRLQ GLGSIHSRTN 

       790 
LMEAELLDAD SDF 

« Hide

References

[1]"The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog."
Stone D.M., Hynes M., Armanini M., Swanson T.A., Gu Q., Johnson R.L., Scott M.P., Pennica D., Goddard A., Phillips H., Noll M., Hooper J.E., de Sauvage F., Rosenthal A.
Nature 384:129-134(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U84402 mRNA. Translation: AAB41789.1.
RefSeqNP_036939.1. NM_012807.1.
UniGeneRn.44429.

3D structure databases

ProteinModelPortalP97698.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000011356.

Chemistry

ChEMBLCHEMBL1795175.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP97698.

Proteomic databases

PaxDbP97698.
PRIDEP97698.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25273.
KEGGrno:25273.
UCSCRGD:3726. rat.

Organism-specific databases

CTD6608.
RGD3726. Smo.

Phylogenomic databases

eggNOGNOG312505.
HOGENOMHOG000252964.
HOVERGENHBG000352.
InParanoidP97698.
KOK06226.
PhylomeDBP97698.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.

Gene expression databases

GenevestigatorP97698.

Family and domain databases

Gene3D1.10.2000.10. 1 hit.
InterProIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR017981. GPCR_2-like.
IPR026544. SMO.
[Graphical view]
PANTHERPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF35. PTHR11309:SF35. 1 hit.
PfamPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSPR00489. FRIZZLED.
SMARTSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMSSF63501. SSF63501. 1 hit.
PROSITEPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio605965.
PROP97698.

Entry information

Entry nameSMO_RAT
AccessionPrimary (citable) accession number: P97698
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: June 11, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries