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Protein

Inositol monophosphatase 1

Gene

Impa1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Has broad substrate specificity and can use myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates (By similarity). Is equally active with myo-inositol monophosphate and D-galactose 1-phosphate.By similarity1 Publication

Catalytic activityi

Myo-inositol phosphate + H2O = myo-inositol + phosphate.1 Publication
Alpha-D-galactose 1-phosphate + H2O = D-galactose + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activity with myo-inositol monophosphate and D-galactose 1-phosphate is inhibited by Li+, Ca2+ and Mn2+, but also by Mg2+ at concentrations above 3 mM.1 Publication

Pathwayi: myo-inositol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Inositol-3-phosphate synthase 1 (Isyna1)
  2. Inositol monophosphatase 3 (Impad1), Inositol monophosphatase 1 (Impa1), Inositol monophosphatase 2 (Impa2)
This subpathway is part of the pathway myo-inositol biosynthesis, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate, the pathway myo-inositol biosynthesis and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70Magnesium 1; catalyticBy similarity1
Binding sitei70SubstrateBy similarity1
Metal bindingi90Magnesium 1; catalyticBy similarity1
Metal bindingi90Magnesium 2By similarity1
Metal bindingi92Magnesium 1; via carbonyl oxygen; catalyticBy similarity1
Metal bindingi93Magnesium 2By similarity1
Binding sitei213SubstrateBy similarity1
Metal bindingi220Magnesium 2By similarity1
Binding sitei220SubstrateBy similarity1

GO - Molecular functioni

  • inositol monophosphate 1-phosphatase activity Source: RGD
  • inositol monophosphate 3-phosphatase activity Source: UniProtKB-EC
  • inositol monophosphate 4-phosphatase activity Source: UniProtKB-EC
  • inositol monophosphate phosphatase activity Source: UniProtKB
  • lithium ion binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • manganese ion binding Source: UniProtKB

GO - Biological processi

  • inositol biosynthetic process Source: UniProtKB-UniPathway
  • inositol metabolic process Source: GO_Central
  • inositol phosphate dephosphorylation Source: UniProtKB
  • phosphatidylinositol biosynthetic process Source: RGD
  • phosphatidylinositol phosphorylation Source: InterPro
  • response to lithium ion Source: RGD
  • signal transduction Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Lithium, Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.3.25. 5301.
SABIO-RKP97697.
UniPathwayiUPA00823; UER00788.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol monophosphatase 1 (EC:3.1.3.251 Publication)
Short name:
IMP 1
Short name:
IMPase 1
Alternative name(s):
D-galactose 1-phosphate phosphatase1 Publication (EC:3.1.3.941 Publication)
Inositol-1(or 4)-monophosphatase 1
Lithium-sensitive myo-inositol monophosphatase A1
Gene namesi
Name:Impa1
Synonyms:Imp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69254. Impa1.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • axon Source: BHF-UCL
  • cytoplasm Source: GO_Central
  • neuronal cell body Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1293238.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001425171 – 277Inositol monophosphatase 1Add BLAST277

Proteomic databases

PaxDbiP97697.
PRIDEiP97697.

PTM databases

iPTMnetiP97697.
PhosphoSitePlusiP97697.
SwissPalmiP97697.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi249742. 1 interactor.
MINTiMINT-1511588.
STRINGi10116.ENSRNOP00000014274.

Structurei

3D structure databases

ProteinModelPortaliP97697.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni92 – 95Substrate bindingBy similarity4
Regioni194 – 196Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the inositol monophosphatase family.Curated

Phylogenomic databases

eggNOGiKOG2951. Eukaryota.
COG0483. LUCA.
HOGENOMiHOG000282238.
HOVERGENiHBG052123.
InParanoidiP97697.
KOiK01092.
PhylomeDBiP97697.

Family and domain databases

CDDicd01639. IMPase. 1 hit.
InterProiIPR033942. IMPase.
IPR020583. Inositol_monoP_metal-BS.
IPR020552. Inositol_monoPase_Li-sen.
IPR000760. Inositol_monophosphatase-like.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PRINTSiPR00377. IMPHPHTASES.
PR00378. LIIMPHPHTASE.
PROSITEiPS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPWQECMD YAVILARQAG EMIREALKNK MDVMIKSSPA DLVTVTDQKV
60 70 80 90 100
EKMLMSSIKE KYPYHSFIGE ESVASGEKTV FTEQPTWIID PIDGTTNFVH
110 120 130 140 150
RFPFVAVSIG FVVNKEMEFG VVYSCVEDKM YTGRKGKGAF CNGQKLRVSQ
160 170 180 190 200
QEDITKSLLV TELGSSRKPE TLRIVLSNME RLCSIPIHGI RSVGTAAVNM
210 220 230 240 250
CLVATGGADA YYEMGIHCWD MAGAGIIVIE AGGVLLDVTG GPFDLMSRRI
260 270
IAASNIALAE RIAKELEIIP LQRDDES
Length:277
Mass (Da):30,511
Last modified:March 1, 2004 - v2
Checksum:i01A7FFB795D2AAED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84038 mRNA. Translation: AAB63338.2.
RefSeqiNP_114446.1. NM_032057.1.
UniGeneiRn.3975.

Genome annotation databases

GeneIDi83523.
KEGGirno:83523.
UCSCiRGD:69254. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84038 mRNA. Translation: AAB63338.2.
RefSeqiNP_114446.1. NM_032057.1.
UniGeneiRn.3975.

3D structure databases

ProteinModelPortaliP97697.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249742. 1 interactor.
MINTiMINT-1511588.
STRINGi10116.ENSRNOP00000014274.

Chemistry databases

ChEMBLiCHEMBL1293238.

PTM databases

iPTMnetiP97697.
PhosphoSitePlusiP97697.
SwissPalmiP97697.

Proteomic databases

PaxDbiP97697.
PRIDEiP97697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83523.
KEGGirno:83523.
UCSCiRGD:69254. rat.

Organism-specific databases

CTDi3612.
RGDi69254. Impa1.

Phylogenomic databases

eggNOGiKOG2951. Eukaryota.
COG0483. LUCA.
HOGENOMiHOG000282238.
HOVERGENiHBG052123.
InParanoidiP97697.
KOiK01092.
PhylomeDBiP97697.

Enzyme and pathway databases

UniPathwayiUPA00823; UER00788.
BRENDAi3.1.3.25. 5301.
SABIO-RKP97697.

Miscellaneous databases

PROiP97697.

Family and domain databases

CDDicd01639. IMPase. 1 hit.
InterProiIPR033942. IMPase.
IPR020583. Inositol_monoP_metal-BS.
IPR020552. Inositol_monoPase_Li-sen.
IPR000760. Inositol_monophosphatase-like.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PRINTSiPR00377. IMPHPHTASES.
PR00378. LIIMPHPHTASE.
PROSITEiPS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMPA1_RAT
AccessioniPrimary (citable) accession number: P97697
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: March 1, 2004
Last modified: November 30, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.