P97697 (IMPA1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inositol monophosphatase 1
Short name=IMP 1
Short name=IMPase 1
EC=3.1.3.25

Alternative name(s):
Inositol-1(or 4)-monophosphatase 1
Lithium-sensitive myo-inositol monophosphatase A1

Gene names

Name:	Impa1
Synonyms:	Imp

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Can use myo-inositol monophosphates, myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates By similarity.
Catalytic activity	Myo-inositol phosphate + H2O = myo-inositol + phosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Inhibited by Li+ By similarity.
Pathway	Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Lithium Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	phosphatidylinositol biosynthetic process Inferred from mutant phenotype. Source: RGD response to lithium ion Inferred from expression pattern. Source: RGD
Cellular component	axon Inferred from direct assay. Source: BHF-UCL cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell neuronal cell body Inferred from direct assay. Source: BHF-UCL
Molecular function	inositol monophosphate 1-phosphatase activity Inferred from direct assay. Source: RGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Inositol monophosphatase 1		PRO_0000142517
Regions
	Region	92 – 95	4	Substrate binding By similarity
	Region	194 – 196	3	Substrate binding By similarity
Sites
	Metal binding	70	1	Magnesium 1 By similarity
	Metal binding	90	1	Magnesium 1 By similarity
	Metal binding	90	1	Magnesium 2 By similarity
	Metal binding	92	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	93	1	Magnesium 2 By similarity
	Metal binding	220	1	Magnesium 2 By similarity
	Binding site	70	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	220	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97697 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: 01A7FFB795D2AAED	FASTA	277	30,511
	10 20 30 40 50 60 MADPWQECMD YAVILARQAG EMIREALKNK MDVMIKSSPA DLVTVTDQKV EKMLMSSIKE 70 80 90 100 110 120 KYPYHSFIGE ESVASGEKTV FTEQPTWIID PIDGTTNFVH RFPFVAVSIG FVVNKEMEFG 130 140 150 160 170 180 VVYSCVEDKM YTGRKGKGAF CNGQKLRVSQ QEDITKSLLV TELGSSRKPE TLRIVLSNME 190 200 210 220 230 240 RLCSIPIHGI RSVGTAAVNM CLVATGGADA YYEMGIHCWD MAGAGIIVIE AGGVLLDVTG 250 260 270 GPFDLMSRRI IAASNIALAE RIAKELEIIP LQRDDES

References

[1]	"Molecular characterization of coding and untranslated regions of rat cortex lithium-sensitive myo-inositol monophosphatase cDNA." Parthasarathy L., Parthasarathy R., Vadnal R. Gene 191:81-87(1997) [PubMed: 9210592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	Parthasarathy L., Parthasarathy R., Vadnal R. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	Lubec G., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 157-167, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U84038 mRNA. Translation: AAB63338.2.
IPI	IPI00206712.
RefSeq	NP_114446.1. NM_032057.1.
UniGene	Rn.3975.
3D structure databases
ProteinModelPortal	P97697.
SMR	P97697. Positions 3-276.
ModBase	Search...
Protein-protein interaction databases
STRING	P97697.
Proteomic databases
PRIDE	P97697.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	83523.
KEGG	rno:83523.
UCSC	NM_032057. rat.
Organism-specific databases
CTD	3612.
RGD	69254. Impa1.
Phylogenomic databases
GeneTree	ENSGT00390000014699.
HOVERGEN	HBG052123.
InParanoid	P97697.
OrthoDB	EOG42FSJ2.
PhylomeDB	P97697.
Gene expression databases
ArrayExpress	P97697.
Genevestigator	P97697.
GermOnline	ENSRNOG00000010482. Rattus norvegicus.
Family and domain databases
InterPro	IPR020583. Inositol_monoP_metal-BS. IPR020552. Inositol_monoPase_Li-sen. IPR000760. Inositol_monophosphatase. IPR020550. Inositol_monophosphatase_CS. [Graphical view]
KO	K01092.
PANTHER	PTHR20854. Inositol_P. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
PRINTS	PR00377. IMPHPHTASES. PR00378. LIIMPHPHTASE.
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	616003.

Entry information

Entry name

IMPA1_RAT

Accession

Primary (citable) accession number: P97697

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	March 1, 2004
Last modified:	November 16, 2011
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program