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P97696 (CYH3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytohesin-3
Alternative name(s):
PH, SEC7 and coiled-coil domain-containing protein 3
SEC7 homolog C
Short name=rSec7-3
Gene names
Name:Cyth3
Synonyms:Pscd3, Sec7c
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF factors through replacement of GDP with GTP By similarity.

Subunit structure

Interacts with GRASP By similarity.

Subcellular location

Cytoplasmcytosol By similarity. Cell membrane; Peripheral membrane protein. Note: Translocates from the cytosol to membranes enriched in phosphatidylinositol 3,4,5-trisphosphate By similarity.

Tissue specificity

Present in all tissues tested, with highest protein levels in brain and adrenal.

Developmental stage

On embryonic days 15 (E15) and E18, expressed in the cortical plate of the cerebrum. On postnatal days 0 (P0) and P7, a moderate expression is seen in the cerebral neocortex, hippocampal pyramidal and dentate granule cell layers. In the cerebellum, expressed in the cerebellar Purkinje cells. On P14, a decreased expression is seen throughout the brain. On P21, expression is seen in the cerebellar Purkinje cells, dentate granule cells and the hippocampal pyramidal cells. Ref.2

Domain

Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate By similarity.

Autoinhibited by its C-terminal basic region By similarity.

Sequence similarities

Contains 1 PH domain.

Contains 1 SEC7 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400Cytohesin-3
PRO_0000120202

Regions

Domain77 – 206130SEC7
Domain265 – 381117PH
Region273 – 2819Phosphatidylinositol 3,4,5-trisphosphate binding By similarity
Region392 – 4009C-terminal autoinhibitory region By similarity
Coiled coil14 – 6148 Potential

Sites

Binding site2851Phosphatidylinositol 3,4,5-trisphosphate By similarity
Binding site2961Phosphatidylinositol 3,4,5-trisphosphate By similarity
Binding site3061Phosphatidylinositol 3,4,5-trisphosphate By similarity
Binding site3551Phosphatidylinositol 3,4,5-trisphosphate By similarity

Sequences

Sequence LengthMass (Da)Tools
P97696 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: C8C19A2B611CB828

FASTA40046,337
        10         20         30         40         50         60 
MDEGGGGEGG SVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT EIDNLTSVEE 

        70         80         90        100        110        120 
SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED VAQFLYKGEG LNKTVIGDYL 

       130        140        150        160        170        180 
GERDDFNIKV LQAFVELHEF ADLNLVQALR QFLWSFRLPG EAQKIDRMME AFASRYCLCN 

       190        200        210        220        230        240 
PGVFQSTDTC YVLSFAIIML NTSLHNHNVR DKPTAERFIT MNRGINEGGD LPEELLRNLY 

       250        260        270        280        290        300 
ESIKNEPFKI PEDDGNDLTH TFFNPDREGW LLKLGGGRVK TWKRRWFILT DNCLYYFEYT 

       310        320        330        340        350        360 
TDKEPRGIIP LENLSIREVE DPRKPNCFEL YNPSHKGQVI KACKTEADGR VVEGNHVVYR 

       370        380        390        400 
ISAPSPEEKE EWMKSIKASI SRDPFYDMLA TRKRRIANKK 

« Hide

References

[1]"Rat homologues of yeast sec7p."
Telemenakis I., Benseler F., Stenius K., Suedhof T.C., Brose N.
Eur. J. Cell Biol. 74:143-149(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Localization of mRNAs for subfamily of guanine nucleotide-exchange proteins (GEP) for ARFs (ADP-ribosylation factors) in the brain of developing and mature rats under normal and postaxotomy conditions."
Suzuki I., Owada Y., Suzuki R., Yoshimoto T., Kondo H.
Brain Res. Mol. Brain Res. 98:41-50(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U83897 mRNA. Translation: AAB41445.1.
UniGeneRn.10673.

3D structure databases

ProteinModelPortalP97696.
SMRP97696. Positions 63-397.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000001412.

Chemistry

BindingDBP97696.
ChEMBLCHEMBL1914274.

Proteomic databases

PaxDbP97696.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

RGD620399. Cyth3.

Phylogenomic databases

eggNOGCOG5307.
HOGENOMHOG000253023.
HOVERGENHBG002647.
InParanoidP97696.
PhylomeDBP97696.

Gene expression databases

GenevestigatorP97696.

Family and domain databases

Gene3D1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamPF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMSSF48425. SSF48425. 1 hit.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP97696.

Entry information

Entry nameCYH3_RAT
AccessionPrimary (citable) accession number: P97696
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families