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Protein

Cytohesin-3

Gene

Cyth3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF factors through replacement of GDP with GTP (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei285 – 2851Phosphatidylinositol 3,4,5-trisphosphateBy similarity
Binding sitei296 – 2961Phosphatidylinositol 3,4,5-trisphosphateBy similarity
Binding sitei306 – 3061Phosphatidylinositol 3,4,5-trisphosphateBy similarity
Binding sitei355 – 3551Phosphatidylinositol 3,4,5-trisphosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytohesin-3
Alternative name(s):
PH, SEC7 and coiled-coil domain-containing protein 3
SEC7 homolog C
Short name:
rSec7-3
Gene namesi
Name:Cyth3
Synonyms:Pscd3, Sec7c
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620399. Cyth3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Cytohesin-3PRO_0000120202Add
BLAST

Proteomic databases

PaxDbiP97696.

Expressioni

Tissue specificityi

Present in all tissues tested, with highest protein levels in brain and adrenal.

Developmental stagei

On embryonic days 15 (E15) and E18, expressed in the cortical plate of the cerebrum. On postnatal days 0 (P0) and P7, a moderate expression is seen in the cerebral neocortex, hippocampal pyramidal and dentate granule cell layers. In the cerebellum, expressed in the cerebellar Purkinje cells. On P14, a decreased expression is seen throughout the brain. On P21, expression is seen in the cerebellar Purkinje cells, dentate granule cells and the hippocampal pyramidal cells.1 Publication

Gene expression databases

ExpressionAtlasiP97696. baseline and differential.

Interactioni

Subunit structurei

Interacts with GRASP.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001412.

Structurei

3D structure databases

ProteinModelPortaliP97696.
SMRiP97696. Positions 63-397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini77 – 206130SEC7PROSITE-ProRule annotationAdd
BLAST
Domaini265 – 381117PHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni273 – 2819Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity
Regioni392 – 4009C-terminal autoinhibitory regionBy similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili14 – 6148Sequence AnalysisAdd
BLAST

Domaini

Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate.By similarity
Autoinhibited by its C-terminal basic region.By similarity

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SEC7 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5307.
HOGENOMiHOG000253023.
HOVERGENiHBG002647.
InParanoidiP97696.
PhylomeDBiP97696.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEGGGGEGG SVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT
60 70 80 90 100
EIDNLTSVEE SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED
110 120 130 140 150
VAQFLYKGEG LNKTVIGDYL GERDDFNIKV LQAFVELHEF ADLNLVQALR
160 170 180 190 200
QFLWSFRLPG EAQKIDRMME AFASRYCLCN PGVFQSTDTC YVLSFAIIML
210 220 230 240 250
NTSLHNHNVR DKPTAERFIT MNRGINEGGD LPEELLRNLY ESIKNEPFKI
260 270 280 290 300
PEDDGNDLTH TFFNPDREGW LLKLGGGRVK TWKRRWFILT DNCLYYFEYT
310 320 330 340 350
TDKEPRGIIP LENLSIREVE DPRKPNCFEL YNPSHKGQVI KACKTEADGR
360 370 380 390 400
VVEGNHVVYR ISAPSPEEKE EWMKSIKASI SRDPFYDMLA TRKRRIANKK
Length:400
Mass (Da):46,337
Last modified:May 1, 1997 - v1
Checksum:iC8C19A2B611CB828
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83897 mRNA. Translation: AAB41445.1.
UniGeneiRn.10673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83897 mRNA. Translation: AAB41445.1.
UniGeneiRn.10673.

3D structure databases

ProteinModelPortaliP97696.
SMRiP97696. Positions 63-397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001412.

Chemistry

BindingDBiP97696.
ChEMBLiCHEMBL1914274.

Proteomic databases

PaxDbiP97696.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi620399. Cyth3.

Phylogenomic databases

eggNOGiCOG5307.
HOGENOMiHOG000253023.
HOVERGENiHBG002647.
InParanoidiP97696.
PhylomeDBiP97696.

Miscellaneous databases

PROiP97696.

Gene expression databases

ExpressionAtlasiP97696. baseline and differential.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rat homologues of yeast sec7p."
    Telemenakis I., Benseler F., Stenius K., Suedhof T.C., Brose N.
    Eur. J. Cell Biol. 74:143-149(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Localization of mRNAs for subfamily of guanine nucleotide-exchange proteins (GEP) for ARFs (ADP-ribosylation factors) in the brain of developing and mature rats under normal and postaxotomy conditions."
    Suzuki I., Owada Y., Suzuki R., Yoshimoto T., Kondo H.
    Brain Res. Mol. Brain Res. 98:41-50(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCYH3_RAT
AccessioniPrimary (citable) accession number: P97696
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 1997
Last modified: June 24, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.