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P97696

- CYH3_RAT

UniProt

P97696 - CYH3_RAT

Protein

Cytohesin-3

Gene

Cyth3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF factors through replacement of GDP with GTP By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei285 – 2851Phosphatidylinositol 3,4,5-trisphosphateBy similarity
    Binding sitei296 – 2961Phosphatidylinositol 3,4,5-trisphosphateBy similarity
    Binding sitei306 – 3061Phosphatidylinositol 3,4,5-trisphosphateBy similarity
    Binding sitei355 – 3551Phosphatidylinositol 3,4,5-trisphosphateBy similarity

    GO - Molecular functioni

    1. ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
    2. phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB

    GO - Biological processi

    1. establishment of epithelial cell polarity Source: Ensembl
    2. Golgi vesicle transport Source: UniProtKB
    3. positive regulation of cell adhesion Source: Ensembl
    4. positive regulation of GTPase activity Source: GOC
    5. regulation of ARF protein signal transduction Source: InterPro
    6. regulation of cell adhesion Source: RefGenome

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytohesin-3
    Alternative name(s):
    PH, SEC7 and coiled-coil domain-containing protein 3
    SEC7 homolog C
    Short name:
    rSec7-3
    Gene namesi
    Name:Cyth3
    Synonyms:Pscd3, Sec7c
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620399. Cyth3.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Cell membrane; Peripheral membrane protein
    Note: Translocates from the cytosol to membranes enriched in phosphatidylinositol 3,4,5-trisphosphate.By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
    3. plasma membrane Source: UniProtKB
    4. ruffle Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 400400Cytohesin-3PRO_0000120202Add
    BLAST

    Proteomic databases

    PaxDbiP97696.

    Expressioni

    Tissue specificityi

    Present in all tissues tested, with highest protein levels in brain and adrenal.

    Developmental stagei

    On embryonic days 15 (E15) and E18, expressed in the cortical plate of the cerebrum. On postnatal days 0 (P0) and P7, a moderate expression is seen in the cerebral neocortex, hippocampal pyramidal and dentate granule cell layers. In the cerebellum, expressed in the cerebellar Purkinje cells. On P14, a decreased expression is seen throughout the brain. On P21, expression is seen in the cerebellar Purkinje cells, dentate granule cells and the hippocampal pyramidal cells.1 Publication

    Gene expression databases

    GenevestigatoriP97696.

    Interactioni

    Subunit structurei

    Interacts with GRASP.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000001412.

    Structurei

    3D structure databases

    ProteinModelPortaliP97696.
    SMRiP97696. Positions 63-397.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini77 – 206130SEC7PROSITE-ProRule annotationAdd
    BLAST
    Domaini265 – 381117PHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni273 – 2819Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity
    Regioni392 – 4009C-terminal autoinhibitory regionBy similarity

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili14 – 6148Sequence AnalysisAdd
    BLAST

    Domaini

    Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate.By similarity
    Autoinhibited by its C-terminal basic region.By similarity

    Sequence similaritiesi

    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 SEC7 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5307.
    HOGENOMiHOG000253023.
    HOVERGENiHBG002647.
    InParanoidiP97696.
    PhylomeDBiP97696.

    Family and domain databases

    Gene3Di1.10.1000.11. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR023394. Sec7_alpha_orthog.
    IPR000904. Sec7_dom.
    [Graphical view]
    PfamiPF00169. PH. 1 hit.
    PF01369. Sec7. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    SM00222. Sec7. 1 hit.
    [Graphical view]
    SUPFAMiSSF48425. SSF48425. 1 hit.
    PROSITEiPS50003. PH_DOMAIN. 1 hit.
    PS50190. SEC7. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P97696-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDEGGGGEGG SVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT    50
    EIDNLTSVEE SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED 100
    VAQFLYKGEG LNKTVIGDYL GERDDFNIKV LQAFVELHEF ADLNLVQALR 150
    QFLWSFRLPG EAQKIDRMME AFASRYCLCN PGVFQSTDTC YVLSFAIIML 200
    NTSLHNHNVR DKPTAERFIT MNRGINEGGD LPEELLRNLY ESIKNEPFKI 250
    PEDDGNDLTH TFFNPDREGW LLKLGGGRVK TWKRRWFILT DNCLYYFEYT 300
    TDKEPRGIIP LENLSIREVE DPRKPNCFEL YNPSHKGQVI KACKTEADGR 350
    VVEGNHVVYR ISAPSPEEKE EWMKSIKASI SRDPFYDMLA TRKRRIANKK 400
    Length:400
    Mass (Da):46,337
    Last modified:May 1, 1997 - v1
    Checksum:iC8C19A2B611CB828
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U83897 mRNA. Translation: AAB41445.1.
    UniGeneiRn.10673.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U83897 mRNA. Translation: AAB41445.1 .
    UniGenei Rn.10673.

    3D structure databases

    ProteinModelPortali P97696.
    SMRi P97696. Positions 63-397.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000001412.

    Chemistry

    BindingDBi P97696.
    ChEMBLi CHEMBL1914274.

    Proteomic databases

    PaxDbi P97696.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    RGDi 620399. Cyth3.

    Phylogenomic databases

    eggNOGi COG5307.
    HOGENOMi HOG000253023.
    HOVERGENi HBG002647.
    InParanoidi P97696.
    PhylomeDBi P97696.

    Miscellaneous databases

    PROi P97696.

    Gene expression databases

    Genevestigatori P97696.

    Family and domain databases

    Gene3Di 1.10.1000.11. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR023394. Sec7_alpha_orthog.
    IPR000904. Sec7_dom.
    [Graphical view ]
    Pfami PF00169. PH. 1 hit.
    PF01369. Sec7. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    SM00222. Sec7. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48425. SSF48425. 1 hit.
    PROSITEi PS50003. PH_DOMAIN. 1 hit.
    PS50190. SEC7. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rat homologues of yeast sec7p."
      Telemenakis I., Benseler F., Stenius K., Suedhof T.C., Brose N.
      Eur. J. Cell Biol. 74:143-149(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Localization of mRNAs for subfamily of guanine nucleotide-exchange proteins (GEP) for ARFs (ADP-ribosylation factors) in the brain of developing and mature rats under normal and postaxotomy conditions."
      Suzuki I., Owada Y., Suzuki R., Yoshimoto T., Kondo H.
      Brain Res. Mol. Brain Res. 98:41-50(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiCYH3_RAT
    AccessioniPrimary (citable) accession number: P97696
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3