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P97696

- CYH3_RAT

UniProt

P97696 - CYH3_RAT

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Protein

Cytohesin-3

Gene

Cyth3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF factors through replacement of GDP with GTP (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei285 – 2851Phosphatidylinositol 3,4,5-trisphosphateBy similarity
Binding sitei296 – 2961Phosphatidylinositol 3,4,5-trisphosphateBy similarity
Binding sitei306 – 3061Phosphatidylinositol 3,4,5-trisphosphateBy similarity
Binding sitei355 – 3551Phosphatidylinositol 3,4,5-trisphosphateBy similarity

GO - Molecular functioni

  1. ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
  2. phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB

GO - Biological processi

  1. establishment of epithelial cell polarity Source: Ensembl
  2. Golgi vesicle transport Source: UniProtKB
  3. positive regulation of cell adhesion Source: Ensembl
  4. positive regulation of GTPase activity Source: GOC
  5. regulation of ARF protein signal transduction Source: InterPro
  6. regulation of cell adhesion Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytohesin-3
Alternative name(s):
PH, SEC7 and coiled-coil domain-containing protein 3
SEC7 homolog C
Short name:
rSec7-3
Gene namesi
Name:Cyth3
Synonyms:Pscd3, Sec7c
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620399. Cyth3.

Subcellular locationi

Cytoplasmcytosol By similarity. Cell membrane; Peripheral membrane protein
Note: Translocates from the cytosol to membranes enriched in phosphatidylinositol 3,4,5-trisphosphate.By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  3. plasma membrane Source: UniProtKB
  4. ruffle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Cytohesin-3PRO_0000120202Add
BLAST

Proteomic databases

PaxDbiP97696.

Expressioni

Tissue specificityi

Present in all tissues tested, with highest protein levels in brain and adrenal.

Developmental stagei

On embryonic days 15 (E15) and E18, expressed in the cortical plate of the cerebrum. On postnatal days 0 (P0) and P7, a moderate expression is seen in the cerebral neocortex, hippocampal pyramidal and dentate granule cell layers. In the cerebellum, expressed in the cerebellar Purkinje cells. On P14, a decreased expression is seen throughout the brain. On P21, expression is seen in the cerebellar Purkinje cells, dentate granule cells and the hippocampal pyramidal cells.1 Publication

Gene expression databases

ExpressionAtlasiP97696. baseline and differential.
GenevestigatoriP97696.

Interactioni

Subunit structurei

Interacts with GRASP.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001412.

Structurei

3D structure databases

ProteinModelPortaliP97696.
SMRiP97696. Positions 63-397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini77 – 206130SEC7PROSITE-ProRule annotationAdd
BLAST
Domaini265 – 381117PHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni273 – 2819Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity
Regioni392 – 4009C-terminal autoinhibitory regionBy similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili14 – 6148Sequence AnalysisAdd
BLAST

Domaini

Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate.By similarity
Autoinhibited by its C-terminal basic region.By similarity

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SEC7 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5307.
HOGENOMiHOG000253023.
HOVERGENiHBG002647.
InParanoidiP97696.
PhylomeDBiP97696.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97696-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDEGGGGEGG SVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT
60 70 80 90 100
EIDNLTSVEE SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED
110 120 130 140 150
VAQFLYKGEG LNKTVIGDYL GERDDFNIKV LQAFVELHEF ADLNLVQALR
160 170 180 190 200
QFLWSFRLPG EAQKIDRMME AFASRYCLCN PGVFQSTDTC YVLSFAIIML
210 220 230 240 250
NTSLHNHNVR DKPTAERFIT MNRGINEGGD LPEELLRNLY ESIKNEPFKI
260 270 280 290 300
PEDDGNDLTH TFFNPDREGW LLKLGGGRVK TWKRRWFILT DNCLYYFEYT
310 320 330 340 350
TDKEPRGIIP LENLSIREVE DPRKPNCFEL YNPSHKGQVI KACKTEADGR
360 370 380 390 400
VVEGNHVVYR ISAPSPEEKE EWMKSIKASI SRDPFYDMLA TRKRRIANKK
Length:400
Mass (Da):46,337
Last modified:May 1, 1997 - v1
Checksum:iC8C19A2B611CB828
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U83897 mRNA. Translation: AAB41445.1.
UniGeneiRn.10673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U83897 mRNA. Translation: AAB41445.1 .
UniGenei Rn.10673.

3D structure databases

ProteinModelPortali P97696.
SMRi P97696. Positions 63-397.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000001412.

Chemistry

BindingDBi P97696.
ChEMBLi CHEMBL1914274.

Proteomic databases

PaxDbi P97696.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

RGDi 620399. Cyth3.

Phylogenomic databases

eggNOGi COG5307.
HOGENOMi HOG000253023.
HOVERGENi HBG002647.
InParanoidi P97696.
PhylomeDBi P97696.

Miscellaneous databases

PROi P97696.

Gene expression databases

ExpressionAtlasi P97696. baseline and differential.
Genevestigatori P97696.

Family and domain databases

Gene3Di 1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProi IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view ]
Pfami PF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view ]
SMARTi SM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view ]
SUPFAMi SSF48425. SSF48425. 1 hit.
PROSITEi PS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Rat homologues of yeast sec7p."
    Telemenakis I., Benseler F., Stenius K., Suedhof T.C., Brose N.
    Eur. J. Cell Biol. 74:143-149(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Localization of mRNAs for subfamily of guanine nucleotide-exchange proteins (GEP) for ARFs (ADP-ribosylation factors) in the brain of developing and mature rats under normal and postaxotomy conditions."
    Suzuki I., Owada Y., Suzuki R., Yoshimoto T., Kondo H.
    Brain Res. Mol. Brain Res. 98:41-50(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCYH3_RAT
AccessioniPrimary (citable) accession number: P97696
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3