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P97691 (FOXM1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Forkhead box protein M1
Alternative name(s):
INS-1 winged helix
Winged-helix factor from INS-1 cells
Gene names
Name:Foxm1
Synonyms:Win
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length759 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response By similarity.

Subcellular location

Nucleus By similarity.

Tissue specificity

Highly expressed in thymus and testis, but weakly in intestine and lung. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors.

Developmental stage

Expressed from E12 day to neonate in developing pancreas. Also expressed at E14 day in liver, thymus, testis and fat (pregnant mother).

Induction

Activated in S phase.

Post-translational modification

Phosphorylated in M (mitotic) phase. Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair. Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates FOXM1, leading to activation of transcriptional activity and subsequent enhanced expression of key mitotic regulators By similarity.

Sequence similarities

Contains 1 fork-head DNA-binding domain.

Ontologies

Keywords
   Biological processCell cycle
DNA damage
DNA repair
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Molecular functionActivator
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

G2/M transition of mitotic cell cycle

Inferred from sequence or structural similarity. Source: UniProtKB

liver development

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of stress-activated MAPK cascade

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of transcription from RNA polymerase II promoter

Inferred from Biological aspect of Ancestor. Source: RefGenome

pattern specification process

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of cell proliferation

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of Ras protein signal transduction

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of cell cycle arrest

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of sequence-specific DNA binding transcription factor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

response to cAMP

Inferred from expression pattern PubMed 10919260. Source: RGD

response to gonadotropin

Inferred from expression pattern PubMed 10919260. Source: RGD

tissue development

Inferred from Biological aspect of Ancestor. Source: RefGenome

transcription from RNA polymerase II promoter

Inferred from Biological aspect of Ancestor. Source: GOC

vasculogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

transcription factor complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionDNA binding

Inferred from direct assay Ref.1. Source: UniProtKB

DNA binding, bending

Inferred from Biological aspect of Ancestor. Source: RefGenome

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

double-stranded DNA binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

sequence-specific DNA binding

Inferred from direct assay Ref.1. Source: RGD

sequence-specific DNA binding transcription factor activity

Inferred from sequence or structural similarity. Source: UniProtKB

transcription factor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P97691-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P97691-2)

The sequence of this isoform differs from the canonical sequence as follows:
     325-339: Missing.
Isoform 3 (identifier: P97691-3)

The sequence of this isoform differs from the canonical sequence as follows:
     281-281: K → KCWHQAYHKLGPQ
Isoform 4 (identifier: P97691-4)

The sequence of this isoform is not available.
Note: Has been shown to exist only in human so far.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 759759Forkhead box protein M1
PRO_0000091865

Regions

DNA binding234 – 32693Fork-head
Compositional bias479 – 51234Glu/Pro/Ser/Thr-rich

Amino acid modifications

Modified residue3301Phosphoserine By similarity
Modified residue3751Phosphoserine; by CHEK2 By similarity
Modified residue6081Phosphothreonine; by CDK1 By similarity
Modified residue6241Phosphothreonine By similarity
Modified residue7261Phosphoserine; by PLK1 By similarity
Modified residue7351Phosphoserine; by PLK1 By similarity

Natural variations

Alternative sequence2811K → KCWHQAYHKLGPQ in isoform 3.
VSP_001549
Alternative sequence325 – 33915Missing in isoform 2.
VSP_001550

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 77231ED495840709

FASTA75983,672
        10         20         30         40         50         60 
MRTSPRRPLI LKRRRLPLPI QNAPSETSEE EAKRSPGQQE PTQAQASQDV AESSSCKFPA 

        70         80         90        100        110        120 
GIKIINHPTV PNTQVVAIPN NADIQSIITA LTAKGKESGS SGPNKFILIS SGGASSHPPD 

       130        140        150        160        170        180 
PQSQAQTSTD SKRTELITET LGPKPGAKGV PVPKPPGALP RQRQESCGGE AAGCTLDNSL 

       190        200        210        220        230        240 
TNIQWLGKMS SDGLGRCSIK QELEEKENCH LEQNRVKVEA PSRASVSWQD SVSERPPYSY 

       250        260        270        280        290        300 
MAMIQFAINS TERKRMTLKD IYTWIEDHFP YFKHIAKPGW KNSIRHNLSL HDMFVRETSA 

       310        320        330        340        350        360 
NGKVSFWTIH PSANRYLTLD QVFKPLEPGS PQSPEHLESQ QKRPNPELRR NVTIKTELPL 

       370        380        390        400        410        420 
GARRKMKPLL PRVSSYLVPI QFPVNQSLVL QPSVKVPLPL AASLMSSELA RHSKRVRIAP 

       430        440        450        460        470        480 
KVLLSNEGIA PLPATEPMKE EKPLLGEGLL PLLPIQSIKE EVIQPGEDIP HLERPIKVES 

       490        500        510        520        530        540 
PPLEEWPSPC ASVKEELSNS WEDSSCSPTP KPKKSYCGLK SPTRCVSEML VTKRREKREV 

       550        560        570        580        590        600 
SRSRRKQHLQ PPCLDEPELF FSEDSSTFRP AMEILAESSE PAPQLSCPQE EGGPFKTPIK 

       610        620        630        640        650        660 
ETLPVSSTPS KSVLSRDPES WRLTPPAKVG GLDFSPVRTP QGAFGPLPDS LGLMELNTTP 

       670        680        690        700        710        720 
LKSVPLFDSP RELLNSEAFD LASDPFSSSP PPHLEAKPGS PELQVPSLSA NRSLTEGLVL 

       730        740        750 
DTMNDSLSKI LLDISFPGLE EDPLGPDNIN WSQFIPELR 

« Hide

Isoform 2 [UniParc].

Checksum: 0701DB6C6FD3A057
Show »

FASTA74482,087
Isoform 3 [UniParc].

Checksum: 98771524E7EB23D4
Show »

FASTA77185,122
Isoform 4 (Sequence not available).

References

[1]"Molecular analysis of a novel winged helix protein, WIN. Expression pattern, DNA binding property, and alternative splicing within the DNA binding domain."
Yao K.-M., Sha M., Lu Z., Wong G.G.
J. Biol. Chem. 272:19827-19836(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
Tissue: Insulinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U83112 mRNA. Translation: AAC63594.1.
UniGeneRn.10665.

3D structure databases

ProteinModelPortalP97691.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248662. 1 interaction.
STRING10116.ENSRNOP00000008003.

Proteomic databases

PaxDbP97691.
PRIDEP97691.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:61807. rat. [P97691-1]

Organism-specific databases

RGD61807. Foxm1.

Phylogenomic databases

eggNOGCOG5025.
HOGENOMHOG000112633.
HOVERGENHBG051652.

Gene expression databases

GenevestigatorP97691.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSPR00053. FORKHEAD.
SMARTSM00339. FH. 1 hit.
[Graphical view]
PROSITEPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio611478.

Entry information

Entry nameFOXM1_RAT
AccessionPrimary (citable) accession number: P97691
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families