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Protein

Forkhead box protein M1

Gene

Foxm1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi234 – 32693Fork-headPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead box protein M1
Alternative name(s):
INS-1 winged helix
Winged-helix factor from INS-1 cells
Gene namesi
Name:Foxm1
Synonyms:Win
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61807. Foxm1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 759759Forkhead box protein M1PRO_0000091865Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei330 – 3301PhosphoserineBy similarity
Modified residuei375 – 3751Phosphoserine; by CHEK2By similarity
Modified residuei608 – 6081Phosphothreonine; by CDK1By similarity
Modified residuei624 – 6241PhosphothreonineBy similarity
Modified residuei726 – 7261Phosphoserine; by PLK1By similarity
Modified residuei735 – 7351Phosphoserine; by PLK1By similarity

Post-translational modificationi

Phosphorylated in M (mitotic) phase. Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair. Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates FOXM1, leading to activation of transcriptional activity and subsequent enhanced expression of key mitotic regulators (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP97691.
PRIDEiP97691.

Expressioni

Tissue specificityi

Highly expressed in thymus and testis, but weakly in intestine and lung. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors.

Developmental stagei

Expressed from E12 day to neonate in developing pancreas. Also expressed at E14 day in liver, thymus, testis and fat (pregnant mother).

Inductioni

Activated in S phase.

Gene expression databases

GenevisibleiP97691. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008003.

Structurei

3D structure databases

ProteinModelPortaliP97691.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi479 – 51234Glu/Pro/Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5025.
HOGENOMiHOG000112633.
HOVERGENiHBG051652.
InParanoidiP97691.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS_1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97691-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRTSPRRPLI LKRRRLPLPI QNAPSETSEE EAKRSPGQQE PTQAQASQDV
60 70 80 90 100
AESSSCKFPA GIKIINHPTV PNTQVVAIPN NADIQSIITA LTAKGKESGS
110 120 130 140 150
SGPNKFILIS SGGASSHPPD PQSQAQTSTD SKRTELITET LGPKPGAKGV
160 170 180 190 200
PVPKPPGALP RQRQESCGGE AAGCTLDNSL TNIQWLGKMS SDGLGRCSIK
210 220 230 240 250
QELEEKENCH LEQNRVKVEA PSRASVSWQD SVSERPPYSY MAMIQFAINS
260 270 280 290 300
TERKRMTLKD IYTWIEDHFP YFKHIAKPGW KNSIRHNLSL HDMFVRETSA
310 320 330 340 350
NGKVSFWTIH PSANRYLTLD QVFKPLEPGS PQSPEHLESQ QKRPNPELRR
360 370 380 390 400
NVTIKTELPL GARRKMKPLL PRVSSYLVPI QFPVNQSLVL QPSVKVPLPL
410 420 430 440 450
AASLMSSELA RHSKRVRIAP KVLLSNEGIA PLPATEPMKE EKPLLGEGLL
460 470 480 490 500
PLLPIQSIKE EVIQPGEDIP HLERPIKVES PPLEEWPSPC ASVKEELSNS
510 520 530 540 550
WEDSSCSPTP KPKKSYCGLK SPTRCVSEML VTKRREKREV SRSRRKQHLQ
560 570 580 590 600
PPCLDEPELF FSEDSSTFRP AMEILAESSE PAPQLSCPQE EGGPFKTPIK
610 620 630 640 650
ETLPVSSTPS KSVLSRDPES WRLTPPAKVG GLDFSPVRTP QGAFGPLPDS
660 670 680 690 700
LGLMELNTTP LKSVPLFDSP RELLNSEAFD LASDPFSSSP PPHLEAKPGS
710 720 730 740 750
PELQVPSLSA NRSLTEGLVL DTMNDSLSKI LLDISFPGLE EDPLGPDNIN

WSQFIPELR
Length:759
Mass (Da):83,672
Last modified:December 1, 2000 - v2
Checksum:i77231ED495840709
GO
Isoform 2 (identifier: P97691-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     325-339: Missing.

Show »
Length:744
Mass (Da):82,087
Checksum:i0701DB6C6FD3A057
GO
Isoform 3 (identifier: P97691-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     281-281: K → KCWHQAYHKLGPQ

Show »
Length:771
Mass (Da):85,122
Checksum:i98771524E7EB23D4
GO
Isoform 4 (identifier: P97691-4)

Sequence is not available
Note: Has been shown to exist only in human so far.
Length:
Mass (Da):

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei281 – 2811K → KCWHQAYHKLGPQ in isoform 3. 1 PublicationVSP_001549
Alternative sequencei325 – 33915Missing in isoform 2. 1 PublicationVSP_001550Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83112 mRNA. Translation: AAC63594.1.
UniGeneiRn.10665.

Genome annotation databases

UCSCiRGD:61807. rat. [P97691-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83112 mRNA. Translation: AAC63594.1.
UniGeneiRn.10665.

3D structure databases

ProteinModelPortaliP97691.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008003.

Proteomic databases

PaxDbiP97691.
PRIDEiP97691.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:61807. rat. [P97691-1]

Organism-specific databases

RGDi61807. Foxm1.

Phylogenomic databases

eggNOGiCOG5025.
HOGENOMiHOG000112633.
HOVERGENiHBG051652.
InParanoidiP97691.

Miscellaneous databases

NextBioi611478.
PROiP97691.

Gene expression databases

GenevisibleiP97691. RN.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS_1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular analysis of a novel winged helix protein, WIN. Expression pattern, DNA binding property, and alternative splicing within the DNA binding domain."
    Yao K.-M., Sha M., Lu Z., Wong G.G.
    J. Biol. Chem. 272:19827-19836(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
    Tissue: Insulinoma.

Entry informationi

Entry nameiFOXM1_RAT
AccessioniPrimary (citable) accession number: P97691
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 24, 2015
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.