Forkhead box protein M1 - Rattus norvegicus (Rat)

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P97691 (FOXM1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 111. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Forkhead box protein M1

Alternative name(s):
INS-1 winged helix
Winged-helix factor from INS-1 cells

Gene names

Name:	Foxm1
Synonyms:	Win

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Highly expressed in thymus and testis, but weakly in intestine and lung. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors.
Developmental stage	Expressed from E12 day to neonate in developing pancreas. Also expressed at E14 day in liver, thymus, testis and fat (pregnant mother).
Induction	Activated in S phase.
Post-translational modification	Phosphorylated in M (mitotic) phase. Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair. Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates FOXM1, leading to activation of transcriptional activity and subsequent enhanced expression of key mitotic regulators By similarity.
Sequence similarities	Contains 1 fork-head DNA-binding domain.

Ontologies

Keywords
Biological process	Cell cycle DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW G2/M transition of mitotic cell cycle Inferred from sequence or structural similarity. Source: UniProtKB embryo development Inferred from Biological aspect of Ancestor. Source: RefGenome liver development Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of stress-activated MAPK cascade Inferred from Biological aspect of Ancestor. Source: RefGenome pattern specification process Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of cell proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB regulation of Ras protein signal transduction Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of cell cycle arrest Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of sequence-specific DNA binding transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome response to cAMP Inferred from expression pattern PubMed 10919260. Source: RGD response to gonadotropin stimulus Inferred from expression pattern PubMed 10919260. Source: RGD tissue development Inferred from Biological aspect of Ancestor. Source: RefGenome vasculogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	transcription factor complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	DNA binding, bending Inferred from Biological aspect of Ancestor. Source: RefGenome RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome double-stranded DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome protein domain specific binding Inferred from Biological aspect of Ancestor. Source: RefGenome sequence-specific DNA binding Inferred from direct assay Ref.1. Source: RGD transcription factor binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P97691-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P97691-2) The sequence of this isoform differs from the canonical sequence as follows: 325-339: Missing.

Isoform 3 (identifier: P97691-3) The sequence of this isoform differs from the canonical sequence as follows: 281-281: K → KCWHQAYHKLGPQ

Isoform 4 (identifier: P97691-4) The sequence of this isoform is not available.
Note: Has been shown to exist only in human so far.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 759

759

Forkhead box protein M1

PRO_0000091865

Regions

DNA binding

234 – 326

Fork-head

Compositional bias

479 – 512

Glu/Pro/Ser/Thr-rich

Amino acid modifications

Modified residue

330

Phosphoserine By similarity

Modified residue

375

Phosphoserine; by CHEK2 By similarity

Modified residue

608

Phosphothreonine; by CDK1 By similarity

Modified residue

624

Phosphothreonine By similarity

Modified residue

726

Phosphoserine; by PLK1 By similarity

Modified residue

735

Phosphoserine; by PLK1 By similarity

Natural variations

Alternative sequence

281

K → KCWHQAYHKLGPQ in isoform 3.

VSP_001549

Alternative sequence

325 – 339

Missing in isoform 2.

VSP_001550

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 77231ED495840709
Show »

FASTA

759

83,672

        10         20         30         40         50         60 
MRTSPRRPLI LKRRRLPLPI QNAPSETSEE EAKRSPGQQE PTQAQASQDV AESSSCKFPA 

        70         80         90        100        110        120 
GIKIINHPTV PNTQVVAIPN NADIQSIITA LTAKGKESGS SGPNKFILIS SGGASSHPPD 

       130        140        150        160        170        180 
PQSQAQTSTD SKRTELITET LGPKPGAKGV PVPKPPGALP RQRQESCGGE AAGCTLDNSL 

       190        200        210        220        230        240 
TNIQWLGKMS SDGLGRCSIK QELEEKENCH LEQNRVKVEA PSRASVSWQD SVSERPPYSY 

       250        260        270        280        290        300 
MAMIQFAINS TERKRMTLKD IYTWIEDHFP YFKHIAKPGW KNSIRHNLSL HDMFVRETSA 

       310        320        330        340        350        360 
NGKVSFWTIH PSANRYLTLD QVFKPLEPGS PQSPEHLESQ QKRPNPELRR NVTIKTELPL 

       370        380        390        400        410        420 
GARRKMKPLL PRVSSYLVPI QFPVNQSLVL QPSVKVPLPL AASLMSSELA RHSKRVRIAP 

       430        440        450        460        470        480 
KVLLSNEGIA PLPATEPMKE EKPLLGEGLL PLLPIQSIKE EVIQPGEDIP HLERPIKVES 

       490        500        510        520        530        540 
PPLEEWPSPC ASVKEELSNS WEDSSCSPTP KPKKSYCGLK SPTRCVSEML VTKRREKREV 

       550        560        570        580        590        600 
SRSRRKQHLQ PPCLDEPELF FSEDSSTFRP AMEILAESSE PAPQLSCPQE EGGPFKTPIK 

       610        620        630        640        650        660 
ETLPVSSTPS KSVLSRDPES WRLTPPAKVG GLDFSPVRTP QGAFGPLPDS LGLMELNTTP 

       670        680        690        700        710        720 
LKSVPLFDSP RELLNSEAFD LASDPFSSSP PPHLEAKPGS PELQVPSLSA NRSLTEGLVL 

       730        740        750 
DTMNDSLSKI LLDISFPGLE EDPLGPDNIN WSQFIPELR

« Hide

Isoform 2 [UniParc].

Checksum: 0701DB6C6FD3A057
Show »

FASTA

744

82,087

Isoform 3 [UniParc].

Checksum: 98771524E7EB23D4
Show »

FASTA

771

85,122

Isoform 4 (Sequence not available).

–

References

[1]	"Molecular analysis of a novel winged helix protein, WIN. Expression pattern, DNA binding property, and alternative splicing within the DNA binding domain." Yao K.-M., Sha M., Lu Z., Wong G.G. J. Biol. Chem. 272:19827-19836(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4). Tissue: Insulinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U83112 mRNA. Translation: AAC63594.1.
IPI	IPI00206706. IPI00231447. IPI00231448.
UniGene	Rn.10665.
3D structure databases
ProteinModelPortal	P97691.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000008003.
Proteomic databases
PaxDb	P97691.
PRIDE	P97691.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:61807. rat.
Organism-specific databases
RGD	61807. Foxm1.
Phylogenomic databases
eggNOG	COG5025.
HOGENOM	HOG000112633.
HOVERGEN	HBG051652.
Gene expression databases
ArrayExpress	P97691.
Genevestigator	P97691.
GermOnline	ENSRNOG00000005936. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR001766. TF_fork_head. IPR018122. TF_fork_head_CS. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00250. Fork_head. 1 hit. [Graphical view]
PRINTS	PR00053. FORKHEAD.
SMART	SM00339. FH. 1 hit. [Graphical view]
PROSITE	PS00657. FORK_HEAD_1. 1 hit. PS00658. FORK_HEAD_2. 1 hit. PS50039. FORK_HEAD_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	611478.

Entry information

Entry name

FOXM1_RAT

Accession

Primary (citable) accession number: P97691

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents