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P97686

- NRCAM_RAT

UniProt

P97686 - NRCAM_RAT

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Protein
Neuronal cell adhesion molecule
Gene
Nrcam
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier By similarity.1 Publication

GO - Molecular functioni

  1. ankyrin binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. cell adhesion Source: RGD
  2. clustering of voltage-gated sodium channels Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal cell adhesion molecule
Short name:
Nr-CAM
Alternative name(s):
Ankyrin-binding cell adhesion molecule NrCAM
Neuronal surface protein Bravo
Short name:
rBravo
NgCAM-related cell adhesion molecule
Short name:
Ng-CAM-related
Gene namesi
Name:Nrcam
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3209. Nrcam.

Subcellular locationi

Cell membrane; Single-pass type I membrane protein
Note: Localized to axonal membranes at the node of Ranvier of myelinated axons.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 10771048Extracellular Reviewed prediction
Add
BLAST
Transmembranei1078 – 110023Helical; Reviewed prediction
Add
BLAST
Topological domaini1101 – 1214114Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. axon initial segment Source: BHF-UCL
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed prediction
Add
BLAST
Chaini30 – 12141185Neuronal cell adhesion molecule
PRO_0000015059Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi68 ↔ 123 Reviewed prediction
Glycosylationi83 – 831N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi167 ↔ 218 Reviewed prediction
Glycosylationi223 – 2231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi245 – 2451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi251 – 2511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi276 – 2761N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi292 ↔ 340 Reviewed prediction
Glycosylationi314 – 3141N-linked (GlcNAc...) Reviewed prediction
Glycosylationi377 – 3771N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi382 ↔ 432 Reviewed prediction
Glycosylationi433 – 4331N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi476 ↔ 525 Reviewed prediction
Glycosylationi507 – 5071N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi567 ↔ 616 Reviewed prediction
Glycosylationi619 – 6191N-linked (GlcNAc...) Reviewed prediction
Glycosylationi716 – 7161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi802 – 8021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi858 – 8581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi993 – 9931N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1009 – 10091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1019 – 10191N-linked (GlcNAc...) Reviewed prediction
Modified residuei1181 – 11811Phosphoserine1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP97686.
PRIDEiP97686.

PTM databases

PhosphoSiteiP97686.

Expressioni

Gene expression databases

GenevestigatoriP97686.

Interactioni

Subunit structurei

Probable constituent of a neurofascin/NRCAM/ankyrin-G complex. Interacts with GLDN/gliomedin. Interacts with MYOC By similarity.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP97686.
SMRiP97686. Positions 638-752, 842-957.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 13489Ig-like C2-type 1
Add
BLAST
Domaini141 – 23595Ig-like C2-type 2
Add
BLAST
Domaini267 – 35690Ig-like C2-type 3
Add
BLAST
Domaini361 – 44888Ig-like C2-type 4
Add
BLAST
Domaini454 – 54188Ig-like C2-type 5
Add
BLAST
Domaini545 – 62682Ig-like C2-type 6
Add
BLAST
Domaini649 – 74496Fibronectin type-III 1
Add
BLAST
Domaini746 – 84398Fibronectin type-III 2
Add
BLAST
Domaini848 – 950103Fibronectin type-III 3
Add
BLAST
Domaini954 – 105198Fibronectin type-III 4
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG293951.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
PhylomeDBiP97686.

Family and domain databases

Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
[Graphical view]
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 4 hits.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
SM00409. IG. 1 hit.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P97686-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MQLKTMPKKK PLSAGRAPLF LFLCQMISAL DVPLDPKLLD DLVQPPTITQ     50
QSPKDYIIDP RENIVIQCEA KGKPPPSFSW TRNGTHFDID KDPLVTMKPG 100
SGTLVINIMS EGKAETYEGV YQCTARNERG AAVSNNIVVR PSRSPLWTKE 150
RLEPIILRSG QSLVLPCRPP IGLPPAIIFW MDNSFQRLPQ SERVSQGLNG 200
DLYFSNVLPE DTREDYICYA RFNHTQTIQQ KQPISLKVIS VDELNDTIAA 250
NLSDTEFYGA KSSKERPPTF LTPEGNESHK EELRGNVLSL ECIAEGLPTP 300
VIYWIKEDGT LPVNRTFYRN FKKTLQIIHV SEADSGNYQC IAKNALGAVH 350
HTISVTVKAA PYWIVAPHNL VLSPGENGTL ICRANGNPKP RISWLTNGVP 400
VEIALDDPSR KIDGDTIMFS NVQESSSAVY QCNASNKYGY LLANAFVNVL 450
AEPPRILTSA NTLYQVIANR PALLDCAFFG SPMPTIEWFK GTKGSALHED 500
IYVLHDNGTL EIPVAQKDST GTYTCVARNK LGMAKNEVHL EIKDPTRFIK 550
QPGYAVVQRG SKVSFECKVK HDHTLIPTIL WLKDNGELPN DERFSVDKDH 600
LVVSDVKDED GGTYTCAANT TLDSVSASAV LRVVAPTPTP APIYDVPNPP 650
FDLELTNQLD KSVQLTWTPG DDNNSPITKF IIEYEDAMHE AGLWRHQAEV 700
SGTQTTAQLK LSPYVNYSFR VMAENSIGRS VPSEASEQYL TKAAEPDQNP 750
TAVEGLGTEP DNLVITWKPL NGFQSNGPGL QYKVSWRQKD GDDEWTSVVV 800
ANVSKYIVSG TPTFVPYLIK VQALNDVGFA PEPAAVMGHS GEDLPMVAPG 850
NVRVSVVNST LAEAHWDPVP PKSVRGHLQG YRIYYWKAQS SSKRNRRHIE 900
KKILTFQGSK THGMLPGLQP YSHYVLNVRV VNGKGEGPAS ADRGFHTPEG 950
VPSAPSSLKI VNPTLDSLTL EWDPPSHPNG ILTEYILKYQ PINSTHELGP 1000
LVDLKIPANK TRWTLKNLNF STRYKFYFYA QTSVGSGSQI TEEAITTVDE 1050
GKKAGILPPD VGAGKAMASR QVDIATQGWF IGLMCAVALL ILILLIVCFI 1100
RRNKGGKYPV KEKEDAHADP EIQPMKEDDG TFGEYSDAED HKPLKKGSRT 1150
PSDRTVKKED SDDSLVDYGE GVNGQFNEDG SFIGQYSGKK EKEPAEGNES 1200
SEAPSPVNAM NSFV 1214
Length:1,214
Mass (Da):133,912
Last modified:November 14, 2003 - v2
Checksum:i2E5EFC09F040DE68
GO
Isoform 2 (identifier: P97686-2) [UniParc]FASTAAdd to Basket

Also known as: Nr-CAM 22

The sequence of this isoform differs from the canonical sequence as follows:
     241-259: Missing.

Show »
Length:1,195
Mass (Da):131,842
Checksum:i7CE3EA89E0E645AA
GO
Isoform 3 (identifier: P97686-3) [UniParc]FASTAAdd to Basket

Also known as: Nr-CAM 12

The sequence of this isoform differs from the canonical sequence as follows:
     36-41: Missing.
     635-644: Missing.
     1051-1065: Missing.

Show »
Length:1,183
Mass (Da):130,831
Checksum:iC06E4568EABC5B08
GO

Sequence cautioni

The sequence AAB47755.1 differs from that shown. Reason: Frameshift at position 1.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei36 – 416Missing in isoform 3.
VSP_008931
Alternative sequencei241 – 25919Missing in isoform 2.
VSP_008932Add
BLAST
Alternative sequencei635 – 64410Missing in isoform 3.
VSP_008933
Alternative sequencei1051 – 106515Missing in isoform 3.
VSP_008934Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U81037 mRNA. Translation: AAB47755.1. Frameshift.
UniGeneiRn.10691.

Genome annotation databases

UCSCiRGD:3209. rat. [P97686-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U81037 mRNA. Translation: AAB47755.1 . Frameshift.
UniGenei Rn.10691.

3D structure databases

ProteinModelPortali P97686.
SMRi P97686. Positions 638-752, 842-957.
ModBasei Search...

PTM databases

PhosphoSitei P97686.

Proteomic databases

PaxDbi P97686.
PRIDEi P97686.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:3209. rat. [P97686-1 ]

Organism-specific databases

RGDi 3209. Nrcam.

Phylogenomic databases

eggNOGi NOG293951.
HOGENOMi HOG000231380.
HOVERGENi HBG000144.
PhylomeDBi P97686.

Miscellaneous databases

PROi P97686.

Gene expression databases

Genevestigatori P97686.

Family and domain databases

Gene3Di 2.60.40.10. 10 hits.
InterProi IPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
[Graphical view ]
Pfami PF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 4 hits.
[Graphical view ]
SMARTi SM00060. FN3. 4 hits.
SM00409. IG. 1 hit.
SM00408. IGc2. 5 hits.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 2 hits.
PROSITEi PS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular composition of the node of Ranvier: identification of ankyrin-binding cell adhesion molecules neurofascin (mucin+/third FNIII domain-) and NrCAM at nodal axon segments."
    Davis J.Q., Lambert S., Bennett V.
    J. Cell Biol. 135:1355-1367(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE OF 30-1214 (ISOFORMS 2 AND 3), CHARACTERIZATION.
    Tissue: Brain.
  2. "Gliomedin mediates Schwann cell-axon interaction and the molecular assembly of the nodes of Ranvier."
    Eshed Y., Feinberg K., Poliak S., Sabanay H., Sarig-Nadir O., Spiegel I., Bermingham J.R. Jr., Peles E.
    Neuron 47:215-229(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GLDN.
  3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1181, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNRCAM_RAT
AccessioniPrimary (citable) accession number: P97686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: September 3, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi