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P97686

- NRCAM_RAT

UniProt

P97686 - NRCAM_RAT

Protein

Neuronal cell adhesion molecule

Gene

Nrcam

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (14 Nov 2003)
      Previous versions | rss
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    Functioni

    Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier By similarity.By similarity

    GO - Molecular functioni

    1. ankyrin binding Source: UniProtKB

    GO - Biological processi

    1. cell adhesion Source: RGD
    2. clustering of voltage-gated sodium channels Source: UniProtKB

    Keywords - Biological processi

    Cell adhesion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuronal cell adhesion molecule
    Short name:
    Nr-CAM
    Alternative name(s):
    Ankyrin-binding cell adhesion molecule NrCAM
    Neuronal surface protein Bravo
    Short name:
    rBravo
    NgCAM-related cell adhesion molecule
    Short name:
    Ng-CAM-related
    Gene namesi
    Name:Nrcam
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3209. Nrcam.

    Subcellular locationi

    Cell membrane; Single-pass type I membrane protein
    Note: Localized to axonal membranes at the node of Ranvier of myelinated axons.

    GO - Cellular componenti

    1. axon initial segment Source: BHF-UCL
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 12141185Neuronal cell adhesion moleculePRO_0000015059Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi68 ↔ 123PROSITE-ProRule annotation
    Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi167 ↔ 218PROSITE-ProRule annotation
    Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi276 – 2761N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi292 ↔ 340PROSITE-ProRule annotation
    Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi377 – 3771N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi382 ↔ 432PROSITE-ProRule annotation
    Glycosylationi433 – 4331N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi476 ↔ 525PROSITE-ProRule annotation
    Glycosylationi507 – 5071N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi567 ↔ 616PROSITE-ProRule annotation
    Glycosylationi619 – 6191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi716 – 7161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi802 – 8021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi858 – 8581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi993 – 9931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1009 – 10091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1019 – 10191N-linked (GlcNAc...)Sequence Analysis
    Modified residuei1181 – 11811Phosphoserine1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP97686.
    PRIDEiP97686.

    PTM databases

    PhosphoSiteiP97686.

    Expressioni

    Gene expression databases

    GenevestigatoriP97686.

    Interactioni

    Subunit structurei

    Probable constituent of a neurofascin/NRCAM/ankyrin-G complex. Interacts with GLDN/gliomedin. Interacts with MYOC By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP97686.
    SMRiP97686. Positions 638-752, 842-957.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini30 – 10771048ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1101 – 1214114CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1078 – 110023HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini46 – 13489Ig-like C2-type 1Add
    BLAST
    Domaini141 – 23595Ig-like C2-type 2Add
    BLAST
    Domaini267 – 35690Ig-like C2-type 3Add
    BLAST
    Domaini361 – 44888Ig-like C2-type 4Add
    BLAST
    Domaini454 – 54188Ig-like C2-type 5Add
    BLAST
    Domaini545 – 62682Ig-like C2-type 6Add
    BLAST
    Domaini649 – 74496Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini746 – 84398Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini848 – 950103Fibronectin type-III 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini954 – 105198Fibronectin type-III 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG293951.
    HOGENOMiHOG000231380.
    HOVERGENiHBG000144.
    PhylomeDBiP97686.

    Family and domain databases

    Gene3Di2.60.40.10. 10 hits.
    InterProiIPR003961. Fibronectin_type3.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003006. Ig/MHC_CS.
    IPR013098. Ig_I-set.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    IPR026966. Neurofascin/L1/NrCAM_C.
    [Graphical view]
    PfamiPF13882. Bravo_FIGEY. 1 hit.
    PF00041. fn3. 4 hits.
    PF07679. I-set. 4 hits.
    [Graphical view]
    SMARTiSM00060. FN3. 4 hits.
    SM00409. IG. 1 hit.
    SM00408. IGc2. 5 hits.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 2 hits.
    PROSITEiPS50853. FN3. 4 hits.
    PS50835. IG_LIKE. 6 hits.
    PS00290. IG_MHC. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P97686-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQLKTMPKKK PLSAGRAPLF LFLCQMISAL DVPLDPKLLD DLVQPPTITQ     50
    QSPKDYIIDP RENIVIQCEA KGKPPPSFSW TRNGTHFDID KDPLVTMKPG 100
    SGTLVINIMS EGKAETYEGV YQCTARNERG AAVSNNIVVR PSRSPLWTKE 150
    RLEPIILRSG QSLVLPCRPP IGLPPAIIFW MDNSFQRLPQ SERVSQGLNG 200
    DLYFSNVLPE DTREDYICYA RFNHTQTIQQ KQPISLKVIS VDELNDTIAA 250
    NLSDTEFYGA KSSKERPPTF LTPEGNESHK EELRGNVLSL ECIAEGLPTP 300
    VIYWIKEDGT LPVNRTFYRN FKKTLQIIHV SEADSGNYQC IAKNALGAVH 350
    HTISVTVKAA PYWIVAPHNL VLSPGENGTL ICRANGNPKP RISWLTNGVP 400
    VEIALDDPSR KIDGDTIMFS NVQESSSAVY QCNASNKYGY LLANAFVNVL 450
    AEPPRILTSA NTLYQVIANR PALLDCAFFG SPMPTIEWFK GTKGSALHED 500
    IYVLHDNGTL EIPVAQKDST GTYTCVARNK LGMAKNEVHL EIKDPTRFIK 550
    QPGYAVVQRG SKVSFECKVK HDHTLIPTIL WLKDNGELPN DERFSVDKDH 600
    LVVSDVKDED GGTYTCAANT TLDSVSASAV LRVVAPTPTP APIYDVPNPP 650
    FDLELTNQLD KSVQLTWTPG DDNNSPITKF IIEYEDAMHE AGLWRHQAEV 700
    SGTQTTAQLK LSPYVNYSFR VMAENSIGRS VPSEASEQYL TKAAEPDQNP 750
    TAVEGLGTEP DNLVITWKPL NGFQSNGPGL QYKVSWRQKD GDDEWTSVVV 800
    ANVSKYIVSG TPTFVPYLIK VQALNDVGFA PEPAAVMGHS GEDLPMVAPG 850
    NVRVSVVNST LAEAHWDPVP PKSVRGHLQG YRIYYWKAQS SSKRNRRHIE 900
    KKILTFQGSK THGMLPGLQP YSHYVLNVRV VNGKGEGPAS ADRGFHTPEG 950
    VPSAPSSLKI VNPTLDSLTL EWDPPSHPNG ILTEYILKYQ PINSTHELGP 1000
    LVDLKIPANK TRWTLKNLNF STRYKFYFYA QTSVGSGSQI TEEAITTVDE 1050
    GKKAGILPPD VGAGKAMASR QVDIATQGWF IGLMCAVALL ILILLIVCFI 1100
    RRNKGGKYPV KEKEDAHADP EIQPMKEDDG TFGEYSDAED HKPLKKGSRT 1150
    PSDRTVKKED SDDSLVDYGE GVNGQFNEDG SFIGQYSGKK EKEPAEGNES 1200
    SEAPSPVNAM NSFV 1214
    Length:1,214
    Mass (Da):133,912
    Last modified:November 14, 2003 - v2
    Checksum:i2E5EFC09F040DE68
    GO
    Isoform 2 (identifier: P97686-2) [UniParc]FASTAAdd to Basket

    Also known as: Nr-CAM 22

    The sequence of this isoform differs from the canonical sequence as follows:
         241-259: Missing.

    Show »
    Length:1,195
    Mass (Da):131,842
    Checksum:i7CE3EA89E0E645AA
    GO
    Isoform 3 (identifier: P97686-3) [UniParc]FASTAAdd to Basket

    Also known as: Nr-CAM 12

    The sequence of this isoform differs from the canonical sequence as follows:
         36-41: Missing.
         635-644: Missing.
         1051-1065: Missing.

    Show »
    Length:1,183
    Mass (Da):130,831
    Checksum:iC06E4568EABC5B08
    GO

    Sequence cautioni

    The sequence AAB47755.1 differs from that shown. Reason: Frameshift at position 1.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei36 – 416Missing in isoform 3. CuratedVSP_008931
    Alternative sequencei241 – 25919Missing in isoform 2. CuratedVSP_008932Add
    BLAST
    Alternative sequencei635 – 64410Missing in isoform 3. CuratedVSP_008933
    Alternative sequencei1051 – 106515Missing in isoform 3. CuratedVSP_008934Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U81037 mRNA. Translation: AAB47755.1. Frameshift.
    UniGeneiRn.10691.

    Genome annotation databases

    UCSCiRGD:3209. rat. [P97686-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U81037 mRNA. Translation: AAB47755.1 . Frameshift.
    UniGenei Rn.10691.

    3D structure databases

    ProteinModelPortali P97686.
    SMRi P97686. Positions 638-752, 842-957.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P97686.

    Proteomic databases

    PaxDbi P97686.
    PRIDEi P97686.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:3209. rat. [P97686-1 ]

    Organism-specific databases

    RGDi 3209. Nrcam.

    Phylogenomic databases

    eggNOGi NOG293951.
    HOGENOMi HOG000231380.
    HOVERGENi HBG000144.
    PhylomeDBi P97686.

    Miscellaneous databases

    PROi P97686.

    Gene expression databases

    Genevestigatori P97686.

    Family and domain databases

    Gene3Di 2.60.40.10. 10 hits.
    InterProi IPR003961. Fibronectin_type3.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003006. Ig/MHC_CS.
    IPR013098. Ig_I-set.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    IPR026966. Neurofascin/L1/NrCAM_C.
    [Graphical view ]
    Pfami PF13882. Bravo_FIGEY. 1 hit.
    PF00041. fn3. 4 hits.
    PF07679. I-set. 4 hits.
    [Graphical view ]
    SMARTi SM00060. FN3. 4 hits.
    SM00409. IG. 1 hit.
    SM00408. IGc2. 5 hits.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 2 hits.
    PROSITEi PS50853. FN3. 4 hits.
    PS50835. IG_LIKE. 6 hits.
    PS00290. IG_MHC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular composition of the node of Ranvier: identification of ankyrin-binding cell adhesion molecules neurofascin (mucin+/third FNIII domain-) and NrCAM at nodal axon segments."
      Davis J.Q., Lambert S., Bennett V.
      J. Cell Biol. 135:1355-1367(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE OF 30-1214 (ISOFORMS 2 AND 3), CHARACTERIZATION.
      Tissue: Brain.
    2. "Gliomedin mediates Schwann cell-axon interaction and the molecular assembly of the nodes of Ranvier."
      Eshed Y., Feinberg K., Poliak S., Sabanay H., Sarig-Nadir O., Spiegel I., Bermingham J.R. Jr., Peles E.
      Neuron 47:215-229(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH GLDN.
    3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
      Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
      Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1181, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNRCAM_RAT
    AccessioniPrimary (citable) accession number: P97686
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 14, 2003
    Last sequence update: November 14, 2003
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3