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Protein

Neuronal cell adhesion molecule

Gene

Nrcam

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons (By similarity). Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons (PubMed:16039564). Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: RGD
  • clustering of voltage-gated sodium channels Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-RNO-447038. NrCAM interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal cell adhesion molecule
Short name:
Nr-CAM
Alternative name(s):
Ankyrin-binding cell adhesion molecule NrCAM
Neuronal surface protein Bravo
Short name:
rBravo
NgCAM-related cell adhesion molecule
Short name:
Ng-CAM-related
Gene namesi
Name:Nrcam
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3209. Nrcam.

Subcellular locationi

  • Cell membrane 1 Publication; Single-pass type I membrane protein By similarity
  • Cell projectionaxon 1 Publication
  • Secreted By similarity

  • Note: Localized to axonal membranes at the node of Ranvier of myelinated axons (PubMed:8947556).1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 1077ExtracellularSequence analysisAdd BLAST1048
Transmembranei1078 – 1100HelicalSequence analysisAdd BLAST23
Topological domaini1101 – 1214CytoplasmicSequence analysisAdd BLAST114

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000001505930 – 1214Neuronal cell adhesion moleculeAdd BLAST1185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi68 ↔ 123PROSITE-ProRule annotation
Glycosylationi83N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi167 ↔ 218PROSITE-ProRule annotation
Glycosylationi223N-linked (GlcNAc...)Sequence analysis1
Glycosylationi245N-linked (GlcNAc...)Sequence analysis1
Glycosylationi251N-linked (GlcNAc...)Sequence analysis1
Glycosylationi276N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi292 ↔ 340PROSITE-ProRule annotation
Glycosylationi314N-linked (GlcNAc...)Sequence analysis1
Glycosylationi377N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi382 ↔ 432PROSITE-ProRule annotation
Glycosylationi433N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi476 ↔ 525PROSITE-ProRule annotation
Glycosylationi507N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi567 ↔ 616PROSITE-ProRule annotation
Glycosylationi619N-linked (GlcNAc...)Sequence analysis1
Glycosylationi716N-linked (GlcNAc...)Sequence analysis1
Glycosylationi802N-linked (GlcNAc...)Sequence analysis1
Glycosylationi858N-linked (GlcNAc...)Sequence analysis1
Glycosylationi993N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1009N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1019N-linked (GlcNAc...)Sequence analysis1
Modified residuei1131PhosphothreonineCombined sources1
Modified residuei1135PhosphotyrosineBy similarity1
Modified residuei1136PhosphoserineCombined sources1
Modified residuei1161PhosphoserineBy similarity1
Modified residuei1164PhosphoserineBy similarity1
Modified residuei1181PhosphoserineCombined sources1
Modified residuei1200PhosphoserineCombined sources1
Modified residuei1201PhosphoserineCombined sources1
Modified residuei1205PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP97686.
PRIDEiP97686.

PTM databases

iPTMnetiP97686.
PhosphoSitePlusiP97686.
UniCarbKBiP97686.

Expressioni

Tissue specificityi

Detected in cerebellum Purkinje cells. Detected on nodes of Ranvier and unmyelinated axons in sciatic nerve (at protein level).1 Publication

Interactioni

Subunit structurei

Constituent of a NFASC/NRCAM/ankyrin-G complex. Detected in a complex with CNTN1 and PTPRB. Interacts with MYOC (By similarity). Interacts with GLDN (PubMed:16039564).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044341.

Structurei

3D structure databases

ProteinModelPortaliP97686.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 134Ig-like C2-type 1Add BLAST89
Domaini141 – 235Ig-like C2-type 2Add BLAST95
Domaini267 – 356Ig-like C2-type 3Add BLAST90
Domaini361 – 448Ig-like C2-type 4Add BLAST88
Domaini454 – 541Ig-like C2-type 5Add BLAST88
Domaini545 – 626Ig-like C2-type 6Add BLAST82
Domaini649 – 744Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST96
Domaini746 – 843Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST98
Domaini848 – 950Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST103
Domaini954 – 1051Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST98

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3513. Eukaryota.
ENOG410XSVG. LUCA.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiP97686.
PhylomeDBiP97686.

Family and domain databases

CDDicd00063. FN3. 4 hits.
Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
[Graphical view]
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
SM00409. IG. 6 hits.
SM00408. IGc2. 6 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 6 hits.
SSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97686-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQLKTMPKKK PLSAGRAPLF LFLCQMISAL DVPLDPKLLD DLVQPPTITQ
60 70 80 90 100
QSPKDYIIDP RENIVIQCEA KGKPPPSFSW TRNGTHFDID KDPLVTMKPG
110 120 130 140 150
SGTLVINIMS EGKAETYEGV YQCTARNERG AAVSNNIVVR PSRSPLWTKE
160 170 180 190 200
RLEPIILRSG QSLVLPCRPP IGLPPAIIFW MDNSFQRLPQ SERVSQGLNG
210 220 230 240 250
DLYFSNVLPE DTREDYICYA RFNHTQTIQQ KQPISLKVIS VDELNDTIAA
260 270 280 290 300
NLSDTEFYGA KSSKERPPTF LTPEGNESHK EELRGNVLSL ECIAEGLPTP
310 320 330 340 350
VIYWIKEDGT LPVNRTFYRN FKKTLQIIHV SEADSGNYQC IAKNALGAVH
360 370 380 390 400
HTISVTVKAA PYWIVAPHNL VLSPGENGTL ICRANGNPKP RISWLTNGVP
410 420 430 440 450
VEIALDDPSR KIDGDTIMFS NVQESSSAVY QCNASNKYGY LLANAFVNVL
460 470 480 490 500
AEPPRILTSA NTLYQVIANR PALLDCAFFG SPMPTIEWFK GTKGSALHED
510 520 530 540 550
IYVLHDNGTL EIPVAQKDST GTYTCVARNK LGMAKNEVHL EIKDPTRFIK
560 570 580 590 600
QPGYAVVQRG SKVSFECKVK HDHTLIPTIL WLKDNGELPN DERFSVDKDH
610 620 630 640 650
LVVSDVKDED GGTYTCAANT TLDSVSASAV LRVVAPTPTP APIYDVPNPP
660 670 680 690 700
FDLELTNQLD KSVQLTWTPG DDNNSPITKF IIEYEDAMHE AGLWRHQAEV
710 720 730 740 750
SGTQTTAQLK LSPYVNYSFR VMAENSIGRS VPSEASEQYL TKAAEPDQNP
760 770 780 790 800
TAVEGLGTEP DNLVITWKPL NGFQSNGPGL QYKVSWRQKD GDDEWTSVVV
810 820 830 840 850
ANVSKYIVSG TPTFVPYLIK VQALNDVGFA PEPAAVMGHS GEDLPMVAPG
860 870 880 890 900
NVRVSVVNST LAEAHWDPVP PKSVRGHLQG YRIYYWKAQS SSKRNRRHIE
910 920 930 940 950
KKILTFQGSK THGMLPGLQP YSHYVLNVRV VNGKGEGPAS ADRGFHTPEG
960 970 980 990 1000
VPSAPSSLKI VNPTLDSLTL EWDPPSHPNG ILTEYILKYQ PINSTHELGP
1010 1020 1030 1040 1050
LVDLKIPANK TRWTLKNLNF STRYKFYFYA QTSVGSGSQI TEEAITTVDE
1060 1070 1080 1090 1100
GKKAGILPPD VGAGKAMASR QVDIATQGWF IGLMCAVALL ILILLIVCFI
1110 1120 1130 1140 1150
RRNKGGKYPV KEKEDAHADP EIQPMKEDDG TFGEYSDAED HKPLKKGSRT
1160 1170 1180 1190 1200
PSDRTVKKED SDDSLVDYGE GVNGQFNEDG SFIGQYSGKK EKEPAEGNES
1210
SEAPSPVNAM NSFV
Length:1,214
Mass (Da):133,912
Last modified:November 14, 2003 - v2
Checksum:i2E5EFC09F040DE68
GO
Isoform 2 (identifier: P97686-2) [UniParc]FASTAAdd to basket
Also known as: Nr-CAM 22

The sequence of this isoform differs from the canonical sequence as follows:
     241-259: Missing.

Show »
Length:1,195
Mass (Da):131,842
Checksum:i7CE3EA89E0E645AA
GO
Isoform 3 (identifier: P97686-3) [UniParc]FASTAAdd to basket
Also known as: Nr-CAM 12

The sequence of this isoform differs from the canonical sequence as follows:
     36-41: Missing.
     635-644: Missing.
     1051-1065: Missing.

Show »
Length:1,183
Mass (Da):130,831
Checksum:iC06E4568EABC5B08
GO

Sequence cautioni

The sequence AAB47755 differs from that shown. Reason: Frameshift at position 1.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00893136 – 41Missing in isoform 3. Curated6
Alternative sequenceiVSP_008932241 – 259Missing in isoform 2. CuratedAdd BLAST19
Alternative sequenceiVSP_008933635 – 644Missing in isoform 3. Curated10
Alternative sequenceiVSP_0089341051 – 1065Missing in isoform 3. CuratedAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81037 mRNA. Translation: AAB47755.1. Frameshift.
UniGeneiRn.10691.

Genome annotation databases

UCSCiRGD:3209. rat. [P97686-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81037 mRNA. Translation: AAB47755.1. Frameshift.
UniGeneiRn.10691.

3D structure databases

ProteinModelPortaliP97686.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044341.

PTM databases

iPTMnetiP97686.
PhosphoSitePlusiP97686.
UniCarbKBiP97686.

Proteomic databases

PaxDbiP97686.
PRIDEiP97686.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:3209. rat. [P97686-1]

Organism-specific databases

RGDi3209. Nrcam.

Phylogenomic databases

eggNOGiKOG3513. Eukaryota.
ENOG410XSVG. LUCA.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiP97686.
PhylomeDBiP97686.

Enzyme and pathway databases

ReactomeiR-RNO-447038. NrCAM interactions.

Miscellaneous databases

PROiP97686.

Family and domain databases

CDDicd00063. FN3. 4 hits.
Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
[Graphical view]
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
SM00409. IG. 6 hits.
SM00408. IGc2. 6 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 6 hits.
SSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNRCAM_RAT
AccessioniPrimary (citable) accession number: P97686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.