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P97686

- NRCAM_RAT

UniProt

P97686 - NRCAM_RAT

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Protein

Neuronal cell adhesion molecule

Gene

Nrcam

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier (By similarity).By similarity

GO - Molecular functioni

  1. ankyrin binding Source: UniProtKB

GO - Biological processi

  1. cell adhesion Source: RGD
  2. clustering of voltage-gated sodium channels Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal cell adhesion molecule
Short name:
Nr-CAM
Alternative name(s):
Ankyrin-binding cell adhesion molecule NrCAM
Neuronal surface protein Bravo
Short name:
rBravo
NgCAM-related cell adhesion molecule
Short name:
Ng-CAM-related
Gene namesi
Name:Nrcam
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3209. Nrcam.

Subcellular locationi

Cell membrane; Single-pass type I membrane protein
Note: Localized to axonal membranes at the node of Ranvier of myelinated axons.

GO - Cellular componenti

  1. axon initial segment Source: BHF-UCL
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Chaini30 – 12141185Neuronal cell adhesion moleculePRO_0000015059Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi68 ↔ 123PROSITE-ProRule annotation
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi167 ↔ 218PROSITE-ProRule annotation
Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi276 – 2761N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi292 ↔ 340PROSITE-ProRule annotation
Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi377 – 3771N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi382 ↔ 432PROSITE-ProRule annotation
Glycosylationi433 – 4331N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi476 ↔ 525PROSITE-ProRule annotation
Glycosylationi507 – 5071N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi567 ↔ 616PROSITE-ProRule annotation
Glycosylationi619 – 6191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi716 – 7161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi802 – 8021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi858 – 8581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi993 – 9931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1009 – 10091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1019 – 10191N-linked (GlcNAc...)Sequence Analysis
Modified residuei1181 – 11811Phosphoserine1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP97686.
PRIDEiP97686.

PTM databases

PhosphoSiteiP97686.

Expressioni

Gene expression databases

GenevestigatoriP97686.

Interactioni

Subunit structurei

Probable constituent of a neurofascin/NRCAM/ankyrin-G complex. Interacts with GLDN/gliomedin. Interacts with MYOC (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP97686.
SMRiP97686. Positions 638-752, 842-957.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 10771048ExtracellularSequence AnalysisAdd
BLAST
Topological domaini1101 – 1214114CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1078 – 110023HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 13489Ig-like C2-type 1Add
BLAST
Domaini141 – 23595Ig-like C2-type 2Add
BLAST
Domaini267 – 35690Ig-like C2-type 3Add
BLAST
Domaini361 – 44888Ig-like C2-type 4Add
BLAST
Domaini454 – 54188Ig-like C2-type 5Add
BLAST
Domaini545 – 62682Ig-like C2-type 6Add
BLAST
Domaini649 – 74496Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini746 – 84398Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini848 – 950103Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini954 – 105198Fibronectin type-III 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG293951.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiP97686.
PhylomeDBiP97686.

Family and domain databases

Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
[Graphical view]
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 4 hits.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
SM00409. IG. 1 hit.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P97686-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQLKTMPKKK PLSAGRAPLF LFLCQMISAL DVPLDPKLLD DLVQPPTITQ
60 70 80 90 100
QSPKDYIIDP RENIVIQCEA KGKPPPSFSW TRNGTHFDID KDPLVTMKPG
110 120 130 140 150
SGTLVINIMS EGKAETYEGV YQCTARNERG AAVSNNIVVR PSRSPLWTKE
160 170 180 190 200
RLEPIILRSG QSLVLPCRPP IGLPPAIIFW MDNSFQRLPQ SERVSQGLNG
210 220 230 240 250
DLYFSNVLPE DTREDYICYA RFNHTQTIQQ KQPISLKVIS VDELNDTIAA
260 270 280 290 300
NLSDTEFYGA KSSKERPPTF LTPEGNESHK EELRGNVLSL ECIAEGLPTP
310 320 330 340 350
VIYWIKEDGT LPVNRTFYRN FKKTLQIIHV SEADSGNYQC IAKNALGAVH
360 370 380 390 400
HTISVTVKAA PYWIVAPHNL VLSPGENGTL ICRANGNPKP RISWLTNGVP
410 420 430 440 450
VEIALDDPSR KIDGDTIMFS NVQESSSAVY QCNASNKYGY LLANAFVNVL
460 470 480 490 500
AEPPRILTSA NTLYQVIANR PALLDCAFFG SPMPTIEWFK GTKGSALHED
510 520 530 540 550
IYVLHDNGTL EIPVAQKDST GTYTCVARNK LGMAKNEVHL EIKDPTRFIK
560 570 580 590 600
QPGYAVVQRG SKVSFECKVK HDHTLIPTIL WLKDNGELPN DERFSVDKDH
610 620 630 640 650
LVVSDVKDED GGTYTCAANT TLDSVSASAV LRVVAPTPTP APIYDVPNPP
660 670 680 690 700
FDLELTNQLD KSVQLTWTPG DDNNSPITKF IIEYEDAMHE AGLWRHQAEV
710 720 730 740 750
SGTQTTAQLK LSPYVNYSFR VMAENSIGRS VPSEASEQYL TKAAEPDQNP
760 770 780 790 800
TAVEGLGTEP DNLVITWKPL NGFQSNGPGL QYKVSWRQKD GDDEWTSVVV
810 820 830 840 850
ANVSKYIVSG TPTFVPYLIK VQALNDVGFA PEPAAVMGHS GEDLPMVAPG
860 870 880 890 900
NVRVSVVNST LAEAHWDPVP PKSVRGHLQG YRIYYWKAQS SSKRNRRHIE
910 920 930 940 950
KKILTFQGSK THGMLPGLQP YSHYVLNVRV VNGKGEGPAS ADRGFHTPEG
960 970 980 990 1000
VPSAPSSLKI VNPTLDSLTL EWDPPSHPNG ILTEYILKYQ PINSTHELGP
1010 1020 1030 1040 1050
LVDLKIPANK TRWTLKNLNF STRYKFYFYA QTSVGSGSQI TEEAITTVDE
1060 1070 1080 1090 1100
GKKAGILPPD VGAGKAMASR QVDIATQGWF IGLMCAVALL ILILLIVCFI
1110 1120 1130 1140 1150
RRNKGGKYPV KEKEDAHADP EIQPMKEDDG TFGEYSDAED HKPLKKGSRT
1160 1170 1180 1190 1200
PSDRTVKKED SDDSLVDYGE GVNGQFNEDG SFIGQYSGKK EKEPAEGNES
1210
SEAPSPVNAM NSFV
Length:1,214
Mass (Da):133,912
Last modified:November 14, 2003 - v2
Checksum:i2E5EFC09F040DE68
GO
Isoform 2 (identifier: P97686-2) [UniParc]FASTAAdd to Basket

Also known as: Nr-CAM 22

The sequence of this isoform differs from the canonical sequence as follows:
     241-259: Missing.

Show »
Length:1,195
Mass (Da):131,842
Checksum:i7CE3EA89E0E645AA
GO
Isoform 3 (identifier: P97686-3) [UniParc]FASTAAdd to Basket

Also known as: Nr-CAM 12

The sequence of this isoform differs from the canonical sequence as follows:
     36-41: Missing.
     635-644: Missing.
     1051-1065: Missing.

Show »
Length:1,183
Mass (Da):130,831
Checksum:iC06E4568EABC5B08
GO

Sequence cautioni

The sequence AAB47755.1 differs from that shown. Reason: Frameshift at position 1.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei36 – 416Missing in isoform 3. CuratedVSP_008931
Alternative sequencei241 – 25919Missing in isoform 2. CuratedVSP_008932Add
BLAST
Alternative sequencei635 – 64410Missing in isoform 3. CuratedVSP_008933
Alternative sequencei1051 – 106515Missing in isoform 3. CuratedVSP_008934Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U81037 mRNA. Translation: AAB47755.1. Frameshift.
UniGeneiRn.10691.

Genome annotation databases

UCSCiRGD:3209. rat. [P97686-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U81037 mRNA. Translation: AAB47755.1 . Frameshift.
UniGenei Rn.10691.

3D structure databases

ProteinModelPortali P97686.
SMRi P97686. Positions 638-752, 842-957.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P97686.

Proteomic databases

PaxDbi P97686.
PRIDEi P97686.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:3209. rat. [P97686-1 ]

Organism-specific databases

RGDi 3209. Nrcam.

Phylogenomic databases

eggNOGi NOG293951.
HOGENOMi HOG000231380.
HOVERGENi HBG000144.
InParanoidi P97686.
PhylomeDBi P97686.

Miscellaneous databases

PROi P97686.

Gene expression databases

Genevestigatori P97686.

Family and domain databases

Gene3Di 2.60.40.10. 10 hits.
InterProi IPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
[Graphical view ]
Pfami PF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 4 hits.
[Graphical view ]
SMARTi SM00060. FN3. 4 hits.
SM00409. IG. 1 hit.
SM00408. IGc2. 5 hits.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 2 hits.
PROSITEi PS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular composition of the node of Ranvier: identification of ankyrin-binding cell adhesion molecules neurofascin (mucin+/third FNIII domain-) and NrCAM at nodal axon segments."
    Davis J.Q., Lambert S., Bennett V.
    J. Cell Biol. 135:1355-1367(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE OF 30-1214 (ISOFORMS 2 AND 3), CHARACTERIZATION.
    Tissue: Brain.
  2. "Gliomedin mediates Schwann cell-axon interaction and the molecular assembly of the nodes of Ranvier."
    Eshed Y., Feinberg K., Poliak S., Sabanay H., Sarig-Nadir O., Spiegel I., Bermingham J.R. Jr., Peles E.
    Neuron 47:215-229(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GLDN.
  3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1181, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNRCAM_RAT
AccessioniPrimary (citable) accession number: P97686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: October 29, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3