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P97686 (NRCAM_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuronal cell adhesion molecule

Short name=Nr-CAM
Alternative name(s):
Ankyrin-binding cell adhesion molecule NrCAM
Neuronal surface protein Bravo
Short name=rBravo
NgCAM-related cell adhesion molecule
Short name=Ng-CAM-related
Gene names
Name:Nrcam
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1214 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier By similarity. Ref.2

Subunit structure

Probable constituent of a neurofascin/NRCAM/ankyrin-G complex. Interacts with GLDN/gliomedin. Interacts with MYOC By similarity. Ref.2

Subcellular location

Cell membrane; Single-pass type I membrane protein. Note: Localized to axonal membranes at the node of Ranvier of myelinated axons.

Sequence similarities

Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.

Contains 4 fibronectin type-III domains.

Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Sequence caution

The sequence AAB47755.1 differs from that shown. Reason: Frameshift at position 1.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P97686-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P97686-2)

Also known as: Nr-CAM 22;

The sequence of this isoform differs from the canonical sequence as follows:
     241-259: Missing.
Isoform 3 (identifier: P97686-3)

Also known as: Nr-CAM 12;

The sequence of this isoform differs from the canonical sequence as follows:
     36-41: Missing.
     635-644: Missing.
     1051-1065: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 12141185Neuronal cell adhesion molecule
PRO_0000015059

Regions

Topological domain30 – 10771048Extracellular Potential
Transmembrane1078 – 110023Helical; Potential
Topological domain1101 – 1214114Cytoplasmic Potential
Domain46 – 13489Ig-like C2-type 1
Domain141 – 23595Ig-like C2-type 2
Domain267 – 35690Ig-like C2-type 3
Domain361 – 44888Ig-like C2-type 4
Domain454 – 54188Ig-like C2-type 5
Domain545 – 62682Ig-like C2-type 6
Domain649 – 74496Fibronectin type-III 1
Domain746 – 84398Fibronectin type-III 2
Domain848 – 950103Fibronectin type-III 3
Domain954 – 105198Fibronectin type-III 4

Amino acid modifications

Modified residue11811Phosphoserine Ref.3
Glycosylation831N-linked (GlcNAc...) Potential
Glycosylation2231N-linked (GlcNAc...) Potential
Glycosylation2451N-linked (GlcNAc...) Potential
Glycosylation2511N-linked (GlcNAc...) Potential
Glycosylation2761N-linked (GlcNAc...) Potential
Glycosylation3141N-linked (GlcNAc...) Potential
Glycosylation3771N-linked (GlcNAc...) Potential
Glycosylation4331N-linked (GlcNAc...) Potential
Glycosylation5071N-linked (GlcNAc...) Potential
Glycosylation6191N-linked (GlcNAc...) Potential
Glycosylation7161N-linked (GlcNAc...) Potential
Glycosylation8021N-linked (GlcNAc...) Potential
Glycosylation8581N-linked (GlcNAc...) Potential
Glycosylation9931N-linked (GlcNAc...) Potential
Glycosylation10091N-linked (GlcNAc...) Potential
Glycosylation10191N-linked (GlcNAc...) Potential
Disulfide bond68 ↔ 123 Potential
Disulfide bond167 ↔ 218 Potential
Disulfide bond292 ↔ 340 Potential
Disulfide bond382 ↔ 432 Potential
Disulfide bond476 ↔ 525 Potential
Disulfide bond567 ↔ 616 Potential

Natural variations

Alternative sequence36 – 416Missing in isoform 3.
VSP_008931
Alternative sequence241 – 25919Missing in isoform 2.
VSP_008932
Alternative sequence635 – 64410Missing in isoform 3.
VSP_008933
Alternative sequence1051 – 106515Missing in isoform 3.
VSP_008934

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 14, 2003. Version 2.
Checksum: 2E5EFC09F040DE68

FASTA1,214133,912
        10         20         30         40         50         60 
MQLKTMPKKK PLSAGRAPLF LFLCQMISAL DVPLDPKLLD DLVQPPTITQ QSPKDYIIDP 

        70         80         90        100        110        120 
RENIVIQCEA KGKPPPSFSW TRNGTHFDID KDPLVTMKPG SGTLVINIMS EGKAETYEGV 

       130        140        150        160        170        180 
YQCTARNERG AAVSNNIVVR PSRSPLWTKE RLEPIILRSG QSLVLPCRPP IGLPPAIIFW 

       190        200        210        220        230        240 
MDNSFQRLPQ SERVSQGLNG DLYFSNVLPE DTREDYICYA RFNHTQTIQQ KQPISLKVIS 

       250        260        270        280        290        300 
VDELNDTIAA NLSDTEFYGA KSSKERPPTF LTPEGNESHK EELRGNVLSL ECIAEGLPTP 

       310        320        330        340        350        360 
VIYWIKEDGT LPVNRTFYRN FKKTLQIIHV SEADSGNYQC IAKNALGAVH HTISVTVKAA 

       370        380        390        400        410        420 
PYWIVAPHNL VLSPGENGTL ICRANGNPKP RISWLTNGVP VEIALDDPSR KIDGDTIMFS 

       430        440        450        460        470        480 
NVQESSSAVY QCNASNKYGY LLANAFVNVL AEPPRILTSA NTLYQVIANR PALLDCAFFG 

       490        500        510        520        530        540 
SPMPTIEWFK GTKGSALHED IYVLHDNGTL EIPVAQKDST GTYTCVARNK LGMAKNEVHL 

       550        560        570        580        590        600 
EIKDPTRFIK QPGYAVVQRG SKVSFECKVK HDHTLIPTIL WLKDNGELPN DERFSVDKDH 

       610        620        630        640        650        660 
LVVSDVKDED GGTYTCAANT TLDSVSASAV LRVVAPTPTP APIYDVPNPP FDLELTNQLD 

       670        680        690        700        710        720 
KSVQLTWTPG DDNNSPITKF IIEYEDAMHE AGLWRHQAEV SGTQTTAQLK LSPYVNYSFR 

       730        740        750        760        770        780 
VMAENSIGRS VPSEASEQYL TKAAEPDQNP TAVEGLGTEP DNLVITWKPL NGFQSNGPGL 

       790        800        810        820        830        840 
QYKVSWRQKD GDDEWTSVVV ANVSKYIVSG TPTFVPYLIK VQALNDVGFA PEPAAVMGHS 

       850        860        870        880        890        900 
GEDLPMVAPG NVRVSVVNST LAEAHWDPVP PKSVRGHLQG YRIYYWKAQS SSKRNRRHIE 

       910        920        930        940        950        960 
KKILTFQGSK THGMLPGLQP YSHYVLNVRV VNGKGEGPAS ADRGFHTPEG VPSAPSSLKI 

       970        980        990       1000       1010       1020 
VNPTLDSLTL EWDPPSHPNG ILTEYILKYQ PINSTHELGP LVDLKIPANK TRWTLKNLNF 

      1030       1040       1050       1060       1070       1080 
STRYKFYFYA QTSVGSGSQI TEEAITTVDE GKKAGILPPD VGAGKAMASR QVDIATQGWF 

      1090       1100       1110       1120       1130       1140 
IGLMCAVALL ILILLIVCFI RRNKGGKYPV KEKEDAHADP EIQPMKEDDG TFGEYSDAED 

      1150       1160       1170       1180       1190       1200 
HKPLKKGSRT PSDRTVKKED SDDSLVDYGE GVNGQFNEDG SFIGQYSGKK EKEPAEGNES 

      1210 
SEAPSPVNAM NSFV 

« Hide

Isoform 2 (Nr-CAM 22) [UniParc].

Checksum: 7CE3EA89E0E645AA
Show »

FASTA1,195131,842
Isoform 3 (Nr-CAM 12) [UniParc].

Checksum: C06E4568EABC5B08
Show »

FASTA1,183130,831

References

« Hide 'large scale' references
[1]"Molecular composition of the node of Ranvier: identification of ankyrin-binding cell adhesion molecules neurofascin (mucin+/third FNIII domain-) and NrCAM at nodal axon segments."
Davis J.Q., Lambert S., Bennett V.
J. Cell Biol. 135:1355-1367(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE OF 30-1214 (ISOFORMS 2 AND 3), CHARACTERIZATION.
Tissue: Brain.
[2]"Gliomedin mediates Schwann cell-axon interaction and the molecular assembly of the nodes of Ranvier."
Eshed Y., Feinberg K., Poliak S., Sabanay H., Sarig-Nadir O., Spiegel I., Bermingham J.R. Jr., Peles E.
Neuron 47:215-229(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GLDN.
[3]"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1181, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U81037 mRNA. Translation: AAB47755.1. Frameshift.
UniGeneRn.10691.

3D structure databases

ProteinModelPortalP97686.
SMRP97686. Positions 638-752, 842-957.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP97686.

Proteomic databases

PaxDbP97686.
PRIDEP97686.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:3209. rat. [P97686-1]

Organism-specific databases

RGD3209. Nrcam.

Phylogenomic databases

eggNOGNOG293951.
HOGENOMHOG000231380.
HOVERGENHBG000144.
PhylomeDBP97686.

Enzyme and pathway databases

ReactomeREACT_195021. Developmental Biology.

Gene expression databases

GenevestigatorP97686.

Family and domain databases

Gene3D2.60.40.10. 10 hits.
InterProIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
[Graphical view]
PfamPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 4 hits.
[Graphical view]
SMARTSM00060. FN3. 4 hits.
SM00409. IG. 1 hit.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 2 hits.
PROSITEPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP97686.

Entry information

Entry nameNRCAM_RAT
AccessionPrimary (citable) accession number: P97686
Entry history
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families