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P97685

- NFASC_RAT

UniProt

P97685 - NFASC_RAT

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Protein
Neurofascin
Gene
Nfasc
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions. Isoform 2/isoform 3 may be responsible for mediating and signaling axon-glial interaction during the early stages of myelination.3 Publications

GO - Molecular functioni

  1. protein domain specific binding Source: RGD
Complete GO annotation...

GO - Biological processi

  1. axon guidance Source: InterPro
  2. cell adhesion Source: UniProtKB-KW
  3. myelination Source: BHF-UCL
  4. peripheral nervous system development Source: BHF-UCL
  5. protein targeting to plasma membrane Source: BHF-UCL
  6. synapse organization Source: InterPro
  7. transmission of nerve impulse Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofascin
Gene namesi
Name:Nfasc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620911. Nfasc.

Subcellular locationi

Cell membrane; Single-pass type I membrane protein
Note: Isoform 1 colocalizes with ankyrin G at the nodes of Ranvier. Isoform 2/ isoform 3 is a glial component of the paranodal axo-glial junction.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 11101086Extracellular Reviewed prediction
Add
BLAST
Transmembranei1111 – 113121Helical; Reviewed prediction
Add
BLAST
Topological domaini1132 – 1240109Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Schwann cell microvillus Source: RGD
  2. axon initial segment Source: BHF-UCL
  3. integral component of membrane Source: UniProtKB-KW
  4. intracellular Source: BHF-UCL
  5. node of Ranvier Source: BHF-UCL
  6. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed prediction
Add
BLAST
Chaini25 – 12401216Neurofascin
PRO_0000015051Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi63 ↔ 118 By similarity
Disulfide bondi162 ↔ 213 By similarity
Disulfide bondi268 ↔ 316 By similarity
Glycosylationi305 – 3051N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi358 ↔ 408 By similarity
Glycosylationi409 – 4091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi446 – 4461N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi452 ↔ 501 Reviewed prediction
Modified residuei481 – 4811Phosphotyrosine By similarity
Glycosylationi483 – 4831N-linked (GlcNAc...) Reviewed prediction
Modified residuei485 – 4851Phosphoserine By similarity
Disulfide bondi543 ↔ 592 Reviewed prediction
Glycosylationi752 – 7521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi778 – 7781N-linked (GlcNAc...) Reviewed prediction
Glycosylationi866 – 8661N-linked (GlcNAc...) Reviewed prediction
Glycosylationi881 – 8811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1015 – 10151N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1026 – 10261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1047 – 10471N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1101 – 11011N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Isoform 2/isoform 3 is phosphorylated at P12. Dephosphorylation is required for ankyrin binding.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP97685.
PRIDEiP97685.

PTM databases

PhosphoSiteiP97685.

Expressioni

Tissue specificityi

Isoform 1 is expressed at Nodes of Ranvier while isoform 2/isoform 3 is expressed in unmyelinated axons.1 Publication

Developmental stagei

Strongly but transiently up-regulated in oligodendrocytes at the onset of myelinogenesis. Once these last have engaged their target exons, expression declines precipitously.

Gene expression databases

GenevestigatoriP97685.

Interactioni

Subunit structurei

Horseshoe-shaped homodimer By similarity. Probable constituent of a neurofascin/NRCAM/ankyrin G complex. Associates with the sodium channel beta-1 (SCN1B) and beta-3 (SCN3B) subunits. Associates to subunit beta-1 in developing axons as early as postanatal day 5, during the period that nodes of Ranvier are forming. Isoform 2/isoform 3 is likely to interact with axonal proteins in close association with CNTNAP1. Interacts with GLDN/gliomedin. Interacts with MYOC By similarity.3 Publications

Protein-protein interaction databases

BioGridi250572. 2 interactions.
MINTiMINT-205368.

Structurei

3D structure databases

ProteinModelPortaliP97685.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 13797Ig-like C2-type 1
Add
BLAST
Domaini143 – 23088Ig-like C2-type 2
Add
BLAST
Domaini244 – 33289Ig-like C2-type 3
Add
BLAST
Domaini337 – 42488Ig-like C2-type 4
Add
BLAST
Domaini429 – 51789Ig-like C2-type 5
Add
BLAST
Domaini521 – 60383Ig-like C2-type 6
Add
BLAST
Domaini630 – 72596Fibronectin type-III 1
Add
BLAST
Domaini727 – 82397Fibronectin type-III 2
Add
BLAST
Domaini827 – 92296Fibronectin type-III 3
Add
BLAST
Domaini1007 – 109993Fibronectin type-III 4
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi913 – 100694Thr-rich
Add
BLAST

Domaini

Homophilic adhesion is primarily mediated by the interaction of the second Ig-like domains By similarity.

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG293951.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiP97685.
KOiK06757.
PhylomeDBiP97685.
TreeFamiTF351098.

Family and domain databases

Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
IPR026965. NFASC.
[Graphical view]
PANTHERiPTHR10489:SF41. PTHR10489:SF41. 1 hit.
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 5 hits.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 4 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 3 hits.
PROSITEiPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P97685-1) [UniParc]FASTAAdd to Basket

Also known as: NF186, 186 kDa isoform

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MARQQAPPWV HVALILFLLS LGGAIEIPMD PSIQNELTQP PTITKQSVKD     50
HIVDPRDNIL IECEAKGNPA PSFHWTRNSR FFNIAKDPRV SMRRRSGTLV 100
IDFRSGGRPE EYEGEYQCFA RNKFGTALSN RIRLQVSKSP LWPKENLDPV 150
VVQEGAPLTL QCNPPPGLPS PVIFWMSSSM EPITQDKRVS QGHNGDLYFS 200
NVMLQDMQTD YSCNARFHFT HTIQQKNPFT LKVLTTRGVA ERTPSFMYPQ 250
GTSSSQMVLR GMDLLLECIA SGVPTPDIAW YKKGGDLPSD KAKFENFNKA 300
LRITNVSEED SGEYFCLASN KMGSIRHTIS VRVKAAPYWL DEPKNLILAP 350
GEDGRLVCRA NGNPKPTVQW LVNGDPLQSA PPNPNREVAG DTIIFRDTQI 400
SSRAVYQCNT SNEHGYLLAN AFVSVLDVPP RMLSPRNQLI RVILYNRTRL 450
DCPFFGSPIP TLRWFKNGQG SNLDGGNYHV YENGSLEIKM IRKEDQGIYT 500
CVATNILGKA ENQVRLEVKD PTRIYRMPED QVAKRGTTVQ LECRVKHDPS 550
LKLTVSWLKD DEPLYIGNRM KKEDDSLTIF GVAERDQGSY TCMASTELDQ 600
DLAKAYLTVL ADQATPTNRL AALPKGRPDR PRDLELTDLA ERSVRLTWIP 650
GDDNNSPITD YVVQFEEDQF QPGVWHDHSK FPGSVNSAVL HLSPYVNYQF 700
RVIAVNEVGS SHPSLPSERY RTSGAPPESN PSDVKGEGTR KNNMEITWTP 750
MNATSAFGPN LRYIVKWRRR ETRETWNNVT VWGSRYVVGQ TPVYVPYEIR 800
VQAENDFGKG PEPETVIGYS GEDLPSAPRR FRVRQPNLET INLEWDHPEH 850
PNGILIGYTL RYVPFNGTKL GKQMVENFSP NQTKFSVQRA DPVSRYRFSL 900
SARTQVGSGE AATEESPTPP NEATPTAAPP TLPPTTVGTT GLVSSTDATA 950
LAATSEATTV PIIPTVVPTT VATTIATTTT TTAAATTTTT TESPPTTTTG 1000
TKIHETAPDE QSIWNVTVLP NSKWANITWK HNFRPGTDFV VEYIDSNHTK 1050
KTVPVKAQAQ PIQLTDLFPG MTYTLRVYSR DNEGISSTVI TFMTSTAYTN 1100
NQTDIATQGW FIGLMCAIAL LVLILLIVCF IKRSRGGKYP VREKKDVPLG 1150
PEDPKEEDGS FDYSDEDNKP LQGSQTSLDG TIKQQESDDS LVDYGEGGEG 1200
QFNEDGSFIG QYTVRKDKEE TEGNESSEAT SPVNAIYSLA 1240
Length:1,240
Mass (Da):138,004
Last modified:November 14, 2003 - v2
Checksum:i636A187BC3772513
GO
Isoform 2 (identifier: P97685-2) [UniParc]FASTAAdd to Basket

Also known as: NF155, 155 kDa isoform

The sequence of this isoform differs from the canonical sequence as follows:
     31-36: Missing.
     236-236: T → NNPYNDSSLRNHPDIYSA
     824-824: L → YPRAAPTEVK...TKEFTTPEGV
     928-1096: Missing.

Show »
Length:1,189
Mass (Da):133,776
Checksum:iC63224BE3EE83B1B
GO
Isoform 3 (identifier: P97685-3) [UniParc]FASTAAdd to Basket

Also known as: NF155, 155 kDa isoform

The sequence of this isoform differs from the canonical sequence as follows:
     31-36: Missing.
     236-236: T → NNPYNDSSLRNHPDIYSA
     611-625: Missing.
     824-824: L → YPRAAPTEVK...TKEFTTPEGV
     928-1096: Missing.

Show »
Length:1,174
Mass (Da):132,228
Checksum:iB9FF83D9A2C20F30
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei31 – 366Missing in isoform 2 and isoform 3.
VSP_050416
Alternative sequencei236 – 2361T → NNPYNDSSLRNHPDIYSA in isoform 2 and isoform 3.
VSP_050417
Alternative sequencei611 – 62515Missing in isoform 3.
VSP_008941Add
BLAST
Alternative sequencei824 – 8241L → YPRAAPTEVKIRVLNSTAIS LQWNRVYPDTVQGQLREYRA YYWRESSLLKNLWVSQKRQQ ASFPGDRPRGVVGRLFPYSN YKLEMVVVNGRGDGPRSETK EFTTPEGV in isoform 2 and isoform 3.
VSP_050418
Alternative sequencei928 – 1096169Missing in isoform 2 and isoform 3.
VSP_050419Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti482 – 4821E → Q in AAB47753. 1 Publication
Sequence conflicti482 – 4821E → Q in AAB47754. 1 Publication
Sequence conflicti675 – 6751W → L in AAB47753. 1 Publication
Sequence conflicti675 – 6751W → L in AAB47754. 1 Publication
Sequence conflicti699 – 6991Q → D in AAB47753. 1 Publication
Sequence conflicti699 – 6991Q → D in AAB47754. 1 Publication
Sequence conflicti763 – 7631Y → YY in AAB47753. 1 Publication
Sequence conflicti763 – 7631Y → YY in AAB47754. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY061639 mRNA. Translation: AAL27854.1.
U81035 mRNA. Translation: AAB47753.1.
U81036 mRNA. Translation: AAB47754.1.
RefSeqiNP_001153785.1. NM_001160313.1. [P97685-2]
NP_001153786.1. NM_001160314.1. [P97685-1]
NP_446361.1. NM_053909.2. [P97685-3]
UniGeneiRn.3048.

Genome annotation databases

GeneIDi116690.
KEGGirno:116690.
UCSCiRGD:620911. rat. [P97685-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY061639 mRNA. Translation: AAL27854.1 .
U81035 mRNA. Translation: AAB47753.1 .
U81036 mRNA. Translation: AAB47754.1 .
RefSeqi NP_001153785.1. NM_001160313.1. [P97685-2 ]
NP_001153786.1. NM_001160314.1. [P97685-1 ]
NP_446361.1. NM_053909.2. [P97685-3 ]
UniGenei Rn.3048.

3D structure databases

ProteinModelPortali P97685.
ModBasei Search...

Protein-protein interaction databases

BioGridi 250572. 2 interactions.
MINTi MINT-205368.

PTM databases

PhosphoSitei P97685.

Proteomic databases

PaxDbi P97685.
PRIDEi P97685.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 116690.
KEGGi rno:116690.
UCSCi RGD:620911. rat. [P97685-1 ]

Organism-specific databases

CTDi 23114.
RGDi 620911. Nfasc.

Phylogenomic databases

eggNOGi NOG293951.
HOGENOMi HOG000231380.
HOVERGENi HBG000144.
InParanoidi P97685.
KOi K06757.
PhylomeDBi P97685.
TreeFami TF351098.

Miscellaneous databases

NextBioi 619556.
PROi P97685.

Gene expression databases

Genevestigatori P97685.

Family and domain databases

Gene3Di 2.60.40.10. 10 hits.
InterProi IPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
IPR026965. NFASC.
[Graphical view ]
PANTHERi PTHR10489:SF41. PTHR10489:SF41. 1 hit.
Pfami PF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 5 hits.
[Graphical view ]
SMARTi SM00060. FN3. 4 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 4 hits.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 3 hits.
PROSITEi PS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Tait S., Collinson J.M., Brophy P.J.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Strain: Wistar.
  2. "Molecular composition of the node of Ranvier: identification of ankyrin-binding cell adhesion molecules neurofascin (mucin+/third FNIII domain-) and NrCAM at nodal axon segments."
    Davis J.Q., Lambert S., Bennett V.
    J. Cell Biol. 135:1355-1367(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-1240 (ISOFORMS 1; 2 AND 3), SUBUNIT.
    Tissue: Brain.
  3. "Transient expression of neurofascin by oligodendrocytes at the onset of myelinogenesis: implications for mechanisms of axon-glial interaction."
    Collinson J.M., Marshall D., Gillespie C.S., Brophy P.J.
    Glia 23:11-23(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF ISOFORMS 2/3.
  4. "An oligodendrocyte cell adhesion molecule at the site of assembly of the paranodal axo-glial junction."
    Tait S., Gunn-Moore F., Collinson J.M., Huang J., Lubetzki C., Pedraza L., Sherman D.L., Colman D.R., Brophy P.J.
    J. Cell Biol. 150:657-666(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF ISOFORMS 1 AND 2/3, INDIRECT ASSOCIATION WITH CNTNAP1, PHOSPHORYLATION, TISSUE SPECIFICITY.
  5. "Sodium channel beta1 and beta3 subunits associate with neurofascin through their extracellular immunoglobulin-like domain."
    Ratcliffe C.F., Westenbroek R.E., Curtis R., Catterall W.A.
    J. Cell Biol. 154:427-434(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SCN1B AND SCN3B.
  6. "Gliomedin mediates Schwann cell-axon interaction and the molecular assembly of the nodes of Ranvier."
    Eshed Y., Feinberg K., Poliak S., Sabanay H., Sarig-Nadir O., Spiegel I., Bermingham J.R. Jr., Peles E.
    Neuron 47:215-229(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GLDN.

Entry informationi

Entry nameiNFASC_RAT
AccessioniPrimary (citable) accession number: P97685
Secondary accession number(s): P97684, Q91Z60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: September 3, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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