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P97685

- NFASC_RAT

UniProt

P97685 - NFASC_RAT

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Protein

Neurofascin

Gene

Nfasc

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions. Isoform 2/isoform 3 may be responsible for mediating and signaling axon-glial interaction during the early stages of myelination.3 Publications

GO - Molecular functioni

  1. protein domain specific binding Source: RGD

GO - Biological processi

  1. axon guidance Source: InterPro
  2. cell adhesion Source: UniProtKB-KW
  3. myelination Source: BHF-UCL
  4. peripheral nervous system development Source: BHF-UCL
  5. protein targeting to plasma membrane Source: BHF-UCL
  6. synapse organization Source: InterPro
  7. transmission of nerve impulse Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_234004. Interaction between L1 and Ankyrins.
REACT_262632. Neurofascin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofascin
Gene namesi
Name:Nfasc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620911. Nfasc.

Subcellular locationi

Cell membrane; Single-pass type I membrane protein
Note: Isoform 1 colocalizes with ankyrin G at the nodes of Ranvier. Isoform 2/ isoform 3 is a glial component of the paranodal axo-glial junction.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 11101086ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1111 – 113121HelicalSequence AnalysisAdd
BLAST
Topological domaini1132 – 1240109CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. axon initial segment Source: BHF-UCL
  2. integral component of membrane Source: UniProtKB-KW
  3. intracellular Source: BHF-UCL
  4. node of Ranvier Source: BHF-UCL
  5. plasma membrane Source: Reactome
  6. Schwann cell microvillus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 12401216NeurofascinPRO_0000015051Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi63 ↔ 118PROSITE-ProRule annotation
Disulfide bondi162 ↔ 213PROSITE-ProRule annotation
Disulfide bondi268 ↔ 316PROSITE-ProRule annotation
Glycosylationi305 – 3051N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi358 ↔ 408PROSITE-ProRule annotation
Glycosylationi409 – 4091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi446 – 4461N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi452 ↔ 501PROSITE-ProRule annotation
Modified residuei481 – 4811PhosphotyrosineBy similarity
Glycosylationi483 – 4831N-linked (GlcNAc...)Sequence Analysis
Modified residuei485 – 4851PhosphoserineBy similarity
Disulfide bondi543 ↔ 592PROSITE-ProRule annotation
Glycosylationi752 – 7521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi778 – 7781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi866 – 8661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi881 – 8811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1015 – 10151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1026 – 10261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1047 – 10471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1101 – 11011N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Isoform 2/isoform 3 is phosphorylated at P12. Dephosphorylation is required for ankyrin binding.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP97685.
PRIDEiP97685.

PTM databases

PhosphoSiteiP97685.

Expressioni

Tissue specificityi

Isoform 1 is expressed at Nodes of Ranvier while isoform 2/isoform 3 is expressed in unmyelinated axons.1 Publication

Developmental stagei

Strongly but transiently up-regulated in oligodendrocytes at the onset of myelinogenesis. Once these last have engaged their target exons, expression declines precipitously.

Gene expression databases

GenevestigatoriP97685.

Interactioni

Subunit structurei

Horseshoe-shaped homodimer (By similarity). Probable constituent of a neurofascin/NRCAM/ankyrin G complex. Associates with the sodium channel beta-1 (SCN1B) and beta-3 (SCN3B) subunits. Associates to subunit beta-1 in developing axons as early as postanatal day 5, during the period that nodes of Ranvier are forming. Isoform 2/isoform 3 is likely to interact with axonal proteins in close association with CNTNAP1. Interacts with GLDN/gliomedin. Interacts with MYOC (By similarity).By similarity

Protein-protein interaction databases

BioGridi250572. 2 interactions.
MINTiMINT-205368.

Structurei

3D structure databases

ProteinModelPortaliP97685.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 13797Ig-like C2-type 1Add
BLAST
Domaini143 – 23088Ig-like C2-type 2Add
BLAST
Domaini244 – 33289Ig-like C2-type 3Add
BLAST
Domaini337 – 42488Ig-like C2-type 4Add
BLAST
Domaini429 – 51789Ig-like C2-type 5Add
BLAST
Domaini521 – 60383Ig-like C2-type 6Add
BLAST
Domaini630 – 72596Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini727 – 82397Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini827 – 92296Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini1007 – 109993Fibronectin type-III 4PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi913 – 100694Thr-richAdd
BLAST

Domaini

Homophilic adhesion is primarily mediated by the interaction of the second Ig-like domains.By similarity

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG293951.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiP97685.
KOiK06757.
PhylomeDBiP97685.
TreeFamiTF351098.

Family and domain databases

Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
IPR026965. NFASC.
[Graphical view]
PANTHERiPTHR10489:SF41. PTHR10489:SF41. 1 hit.
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 5 hits.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 4 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 3 hits.
PROSITEiPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P97685-1) [UniParc]FASTAAdd to Basket

Also known as: NF186, 186 kDa isoform

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARQQAPPWV HVALILFLLS LGGAIEIPMD PSIQNELTQP PTITKQSVKD
60 70 80 90 100
HIVDPRDNIL IECEAKGNPA PSFHWTRNSR FFNIAKDPRV SMRRRSGTLV
110 120 130 140 150
IDFRSGGRPE EYEGEYQCFA RNKFGTALSN RIRLQVSKSP LWPKENLDPV
160 170 180 190 200
VVQEGAPLTL QCNPPPGLPS PVIFWMSSSM EPITQDKRVS QGHNGDLYFS
210 220 230 240 250
NVMLQDMQTD YSCNARFHFT HTIQQKNPFT LKVLTTRGVA ERTPSFMYPQ
260 270 280 290 300
GTSSSQMVLR GMDLLLECIA SGVPTPDIAW YKKGGDLPSD KAKFENFNKA
310 320 330 340 350
LRITNVSEED SGEYFCLASN KMGSIRHTIS VRVKAAPYWL DEPKNLILAP
360 370 380 390 400
GEDGRLVCRA NGNPKPTVQW LVNGDPLQSA PPNPNREVAG DTIIFRDTQI
410 420 430 440 450
SSRAVYQCNT SNEHGYLLAN AFVSVLDVPP RMLSPRNQLI RVILYNRTRL
460 470 480 490 500
DCPFFGSPIP TLRWFKNGQG SNLDGGNYHV YENGSLEIKM IRKEDQGIYT
510 520 530 540 550
CVATNILGKA ENQVRLEVKD PTRIYRMPED QVAKRGTTVQ LECRVKHDPS
560 570 580 590 600
LKLTVSWLKD DEPLYIGNRM KKEDDSLTIF GVAERDQGSY TCMASTELDQ
610 620 630 640 650
DLAKAYLTVL ADQATPTNRL AALPKGRPDR PRDLELTDLA ERSVRLTWIP
660 670 680 690 700
GDDNNSPITD YVVQFEEDQF QPGVWHDHSK FPGSVNSAVL HLSPYVNYQF
710 720 730 740 750
RVIAVNEVGS SHPSLPSERY RTSGAPPESN PSDVKGEGTR KNNMEITWTP
760 770 780 790 800
MNATSAFGPN LRYIVKWRRR ETRETWNNVT VWGSRYVVGQ TPVYVPYEIR
810 820 830 840 850
VQAENDFGKG PEPETVIGYS GEDLPSAPRR FRVRQPNLET INLEWDHPEH
860 870 880 890 900
PNGILIGYTL RYVPFNGTKL GKQMVENFSP NQTKFSVQRA DPVSRYRFSL
910 920 930 940 950
SARTQVGSGE AATEESPTPP NEATPTAAPP TLPPTTVGTT GLVSSTDATA
960 970 980 990 1000
LAATSEATTV PIIPTVVPTT VATTIATTTT TTAAATTTTT TESPPTTTTG
1010 1020 1030 1040 1050
TKIHETAPDE QSIWNVTVLP NSKWANITWK HNFRPGTDFV VEYIDSNHTK
1060 1070 1080 1090 1100
KTVPVKAQAQ PIQLTDLFPG MTYTLRVYSR DNEGISSTVI TFMTSTAYTN
1110 1120 1130 1140 1150
NQTDIATQGW FIGLMCAIAL LVLILLIVCF IKRSRGGKYP VREKKDVPLG
1160 1170 1180 1190 1200
PEDPKEEDGS FDYSDEDNKP LQGSQTSLDG TIKQQESDDS LVDYGEGGEG
1210 1220 1230 1240
QFNEDGSFIG QYTVRKDKEE TEGNESSEAT SPVNAIYSLA
Length:1,240
Mass (Da):138,004
Last modified:November 14, 2003 - v2
Checksum:i636A187BC3772513
GO
Isoform 2 (identifier: P97685-2) [UniParc]FASTAAdd to Basket

Also known as: NF155, 155 kDa isoform

The sequence of this isoform differs from the canonical sequence as follows:
     31-36: Missing.
     236-236: T → NNPYNDSSLRNHPDIYSA
     824-824: L → YPRAAPTEVK...TKEFTTPEGV
     928-1096: Missing.

Show »
Length:1,189
Mass (Da):133,776
Checksum:iC63224BE3EE83B1B
GO
Isoform 3 (identifier: P97685-3) [UniParc]FASTAAdd to Basket

Also known as: NF155, 155 kDa isoform

The sequence of this isoform differs from the canonical sequence as follows:
     31-36: Missing.
     236-236: T → NNPYNDSSLRNHPDIYSA
     611-625: Missing.
     824-824: L → YPRAAPTEVK...TKEFTTPEGV
     928-1096: Missing.

Show »
Length:1,174
Mass (Da):132,228
Checksum:iB9FF83D9A2C20F30
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti482 – 4821E → Q in AAB47753. (PubMed:8947556)Curated
Sequence conflicti482 – 4821E → Q in AAB47754. (PubMed:8947556)Curated
Sequence conflicti675 – 6751W → L in AAB47753. (PubMed:8947556)Curated
Sequence conflicti675 – 6751W → L in AAB47754. (PubMed:8947556)Curated
Sequence conflicti699 – 6991Q → D in AAB47753. (PubMed:8947556)Curated
Sequence conflicti699 – 6991Q → D in AAB47754. (PubMed:8947556)Curated
Sequence conflicti763 – 7631Y → YY in AAB47753. (PubMed:8947556)Curated
Sequence conflicti763 – 7631Y → YY in AAB47754. (PubMed:8947556)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei31 – 366Missing in isoform 2 and isoform 3. 2 PublicationsVSP_050416
Alternative sequencei236 – 2361T → NNPYNDSSLRNHPDIYSA in isoform 2 and isoform 3. 2 PublicationsVSP_050417
Alternative sequencei611 – 62515Missing in isoform 3. 2 PublicationsVSP_008941Add
BLAST
Alternative sequencei824 – 8241L → YPRAAPTEVKIRVLNSTAIS LQWNRVYPDTVQGQLREYRA YYWRESSLLKNLWVSQKRQQ ASFPGDRPRGVVGRLFPYSN YKLEMVVVNGRGDGPRSETK EFTTPEGV in isoform 2 and isoform 3. 2 PublicationsVSP_050418
Alternative sequencei928 – 1096169Missing in isoform 2 and isoform 3. 2 PublicationsVSP_050419Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061639 mRNA. Translation: AAL27854.1.
U81035 mRNA. Translation: AAB47753.1.
U81036 mRNA. Translation: AAB47754.1.
RefSeqiNP_001153785.1. NM_001160313.1. [P97685-2]
NP_001153786.1. NM_001160314.1. [P97685-1]
NP_446361.1. NM_053909.2. [P97685-3]
UniGeneiRn.3048.

Genome annotation databases

GeneIDi116690.
KEGGirno:116690.
UCSCiRGD:620911. rat. [P97685-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061639 mRNA. Translation: AAL27854.1 .
U81035 mRNA. Translation: AAB47753.1 .
U81036 mRNA. Translation: AAB47754.1 .
RefSeqi NP_001153785.1. NM_001160313.1. [P97685-2 ]
NP_001153786.1. NM_001160314.1. [P97685-1 ]
NP_446361.1. NM_053909.2. [P97685-3 ]
UniGenei Rn.3048.

3D structure databases

ProteinModelPortali P97685.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250572. 2 interactions.
MINTi MINT-205368.

PTM databases

PhosphoSitei P97685.

Proteomic databases

PaxDbi P97685.
PRIDEi P97685.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 116690.
KEGGi rno:116690.
UCSCi RGD:620911. rat. [P97685-1 ]

Organism-specific databases

CTDi 23114.
RGDi 620911. Nfasc.

Phylogenomic databases

eggNOGi NOG293951.
HOGENOMi HOG000231380.
HOVERGENi HBG000144.
InParanoidi P97685.
KOi K06757.
PhylomeDBi P97685.
TreeFami TF351098.

Enzyme and pathway databases

Reactomei REACT_234004. Interaction between L1 and Ankyrins.
REACT_262632. Neurofascin interactions.

Miscellaneous databases

NextBioi 619556.
PROi P97685.

Gene expression databases

Genevestigatori P97685.

Family and domain databases

Gene3Di 2.60.40.10. 10 hits.
InterProi IPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
IPR026965. NFASC.
[Graphical view ]
PANTHERi PTHR10489:SF41. PTHR10489:SF41. 1 hit.
Pfami PF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 5 hits.
[Graphical view ]
SMARTi SM00060. FN3. 4 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 4 hits.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 3 hits.
PROSITEi PS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Tait S., Collinson J.M., Brophy P.J.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Strain: Wistar.
  2. "Molecular composition of the node of Ranvier: identification of ankyrin-binding cell adhesion molecules neurofascin (mucin+/third FNIII domain-) and NrCAM at nodal axon segments."
    Davis J.Q., Lambert S., Bennett V.
    J. Cell Biol. 135:1355-1367(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-1240 (ISOFORMS 1; 2 AND 3), SUBUNIT.
    Tissue: Brain.
  3. "Transient expression of neurofascin by oligodendrocytes at the onset of myelinogenesis: implications for mechanisms of axon-glial interaction."
    Collinson J.M., Marshall D., Gillespie C.S., Brophy P.J.
    Glia 23:11-23(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF ISOFORMS 2/3.
  4. "An oligodendrocyte cell adhesion molecule at the site of assembly of the paranodal axo-glial junction."
    Tait S., Gunn-Moore F., Collinson J.M., Huang J., Lubetzki C., Pedraza L., Sherman D.L., Colman D.R., Brophy P.J.
    J. Cell Biol. 150:657-666(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF ISOFORMS 1 AND 2/3, INDIRECT ASSOCIATION WITH CNTNAP1, PHOSPHORYLATION, TISSUE SPECIFICITY.
  5. "Sodium channel beta1 and beta3 subunits associate with neurofascin through their extracellular immunoglobulin-like domain."
    Ratcliffe C.F., Westenbroek R.E., Curtis R., Catterall W.A.
    J. Cell Biol. 154:427-434(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SCN1B AND SCN3B.
  6. "Gliomedin mediates Schwann cell-axon interaction and the molecular assembly of the nodes of Ranvier."
    Eshed Y., Feinberg K., Poliak S., Sabanay H., Sarig-Nadir O., Spiegel I., Bermingham J.R. Jr., Peles E.
    Neuron 47:215-229(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GLDN.

Entry informationi

Entry nameiNFASC_RAT
AccessioniPrimary (citable) accession number: P97685
Secondary accession number(s): P97684, Q91Z60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: November 26, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3