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Protein

Neurofascin

Gene

Nfasc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions. Isoform 2/isoform 3 may be responsible for mediating and signaling axon-glial interaction during the early stages of myelination.3 Publications

GO - Molecular functioni

  • protein domain specific binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-RNO-445095. Interaction between L1 and Ankyrins.
R-RNO-447043. Neurofascin interactions.
R-RNO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofascin
Gene namesi
Name:Nfasc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi620911. Nfasc.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 1110ExtracellularSequence analysisAdd BLAST1086
Transmembranei1111 – 1131HelicalSequence analysisAdd BLAST21
Topological domaini1132 – 1240CytoplasmicSequence analysisAdd BLAST109

GO - Cellular componenti

  • axon initial segment Source: BHF-UCL
  • extracellular exosome Source: Ensembl
  • focal adhesion Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: BHF-UCL
  • myelin sheath Source: Ensembl
  • node of Ranvier Source: BHF-UCL
  • paranodal junction Source: Ensembl
  • paranode region of axon Source: Ensembl
  • plasma membrane Source: Reactome
  • Schwann cell microvillus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000001505125 – 1240NeurofascinAdd BLAST1216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi63 ↔ 118PROSITE-ProRule annotation
Disulfide bondi162 ↔ 213PROSITE-ProRule annotation
Disulfide bondi268 ↔ 316PROSITE-ProRule annotation
Glycosylationi305N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi358 ↔ 408PROSITE-ProRule annotation
Glycosylationi409N-linked (GlcNAc...)Sequence analysis1
Glycosylationi446N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi452 ↔ 501PROSITE-ProRule annotation
Modified residuei481PhosphotyrosineBy similarity1
Glycosylationi483N-linked (GlcNAc...)Sequence analysis1
Modified residuei485PhosphoserineBy similarity1
Disulfide bondi543 ↔ 592PROSITE-ProRule annotation
Glycosylationi752N-linked (GlcNAc...)Sequence analysis1
Glycosylationi778N-linked (GlcNAc...)Sequence analysis1
Glycosylationi866N-linked (GlcNAc...)Sequence analysis1
Glycosylationi881N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1015N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1026N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1047N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1101N-linked (GlcNAc...)Sequence analysis1
Modified residuei1160PhosphoserineBy similarity1
Modified residuei1174PhosphoserineCombined sources1
Modified residuei1187PhosphoserineCombined sources1
Modified residuei1190PhosphoserineCombined sources1
Modified residuei1226PhosphoserineBy similarity1
Modified residuei1227PhosphoserineBy similarity1
Modified residuei1231PhosphoserineBy similarity1

Post-translational modificationi

Isoform 2/isoform 3 is phosphorylated at P12. Dephosphorylation is required for ankyrin binding.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP97685.
PRIDEiP97685.

PTM databases

iPTMnetiP97685.
PhosphoSitePlusiP97685.

Expressioni

Tissue specificityi

Isoform 1 is expressed at Nodes of Ranvier while isoform 2/isoform 3 is expressed in unmyelinated axons.1 Publication

Developmental stagei

Strongly but transiently up-regulated in oligodendrocytes at the onset of myelinogenesis. Once these last have engaged their target exons, expression declines precipitously.

Gene expression databases

ExpressionAtlasiP97685. baseline and differential.
GenevisibleiP97685. RN.

Interactioni

Subunit structurei

Horseshoe-shaped homodimer (By similarity). Probable constituent of a neurofascin/NRCAM/ankyrin G complex. Associates with the sodium channel beta-1 (SCN1B) and beta-3 (SCN3B) subunits. Associates to subunit beta-1 in developing axons as early as postanatal day 5, during the period that nodes of Ranvier are forming. Isoform 2/isoform 3 is likely to interact with axonal proteins in close association with CNTNAP1. Interacts with GLDN/gliomedin. Interacts with MYOC (By similarity).By similarity

GO - Molecular functioni

  • protein domain specific binding Source: RGD

Protein-protein interaction databases

BioGridi250572. 2 interactors.
MINTiMINT-205368.
STRINGi10116.ENSRNOP00000057594.

Structurei

3D structure databases

ProteinModelPortaliP97685.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 137Ig-like C2-type 1Add BLAST97
Domaini143 – 230Ig-like C2-type 2Add BLAST88
Domaini244 – 332Ig-like C2-type 3Add BLAST89
Domaini337 – 424Ig-like C2-type 4Add BLAST88
Domaini429 – 517Ig-like C2-type 5Add BLAST89
Domaini521 – 603Ig-like C2-type 6Add BLAST83
Domaini630 – 725Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST96
Domaini727 – 823Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST97
Domaini827 – 922Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST96
Domaini1007 – 1099Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST93

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi913 – 1006Thr-richAdd BLAST94

Domaini

Homophilic adhesion is primarily mediated by the interaction of the second Ig-like domains.By similarity

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3513. Eukaryota.
ENOG410XSVG. LUCA.
GeneTreeiENSGT00760000118840.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiP97685.
KOiK06757.
OrthoDBiEOG091G00LY.
PhylomeDBiP97685.
TreeFamiTF351098.

Family and domain databases

CDDicd00063. FN3. 4 hits.
Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013151. Immunoglobulin.
IPR026966. Neurofascin/L1/NrCAM_C.
IPR026965. NFASC.
[Graphical view]
PANTHERiPTHR10489:SF715. PTHR10489:SF715. 3 hits.
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 2 hits.
PF00047. ig. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
SM00409. IG. 6 hits.
SM00408. IGc2. 6 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 6 hits.
SSF49265. SSF49265. 3 hits.
PROSITEiPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97685-1) [UniParc]FASTAAdd to basket
Also known as: NF186, 186 kDa isoform

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARQQAPPWV HVALILFLLS LGGAIEIPMD PSIQNELTQP PTITKQSVKD
60 70 80 90 100
HIVDPRDNIL IECEAKGNPA PSFHWTRNSR FFNIAKDPRV SMRRRSGTLV
110 120 130 140 150
IDFRSGGRPE EYEGEYQCFA RNKFGTALSN RIRLQVSKSP LWPKENLDPV
160 170 180 190 200
VVQEGAPLTL QCNPPPGLPS PVIFWMSSSM EPITQDKRVS QGHNGDLYFS
210 220 230 240 250
NVMLQDMQTD YSCNARFHFT HTIQQKNPFT LKVLTTRGVA ERTPSFMYPQ
260 270 280 290 300
GTSSSQMVLR GMDLLLECIA SGVPTPDIAW YKKGGDLPSD KAKFENFNKA
310 320 330 340 350
LRITNVSEED SGEYFCLASN KMGSIRHTIS VRVKAAPYWL DEPKNLILAP
360 370 380 390 400
GEDGRLVCRA NGNPKPTVQW LVNGDPLQSA PPNPNREVAG DTIIFRDTQI
410 420 430 440 450
SSRAVYQCNT SNEHGYLLAN AFVSVLDVPP RMLSPRNQLI RVILYNRTRL
460 470 480 490 500
DCPFFGSPIP TLRWFKNGQG SNLDGGNYHV YENGSLEIKM IRKEDQGIYT
510 520 530 540 550
CVATNILGKA ENQVRLEVKD PTRIYRMPED QVAKRGTTVQ LECRVKHDPS
560 570 580 590 600
LKLTVSWLKD DEPLYIGNRM KKEDDSLTIF GVAERDQGSY TCMASTELDQ
610 620 630 640 650
DLAKAYLTVL ADQATPTNRL AALPKGRPDR PRDLELTDLA ERSVRLTWIP
660 670 680 690 700
GDDNNSPITD YVVQFEEDQF QPGVWHDHSK FPGSVNSAVL HLSPYVNYQF
710 720 730 740 750
RVIAVNEVGS SHPSLPSERY RTSGAPPESN PSDVKGEGTR KNNMEITWTP
760 770 780 790 800
MNATSAFGPN LRYIVKWRRR ETRETWNNVT VWGSRYVVGQ TPVYVPYEIR
810 820 830 840 850
VQAENDFGKG PEPETVIGYS GEDLPSAPRR FRVRQPNLET INLEWDHPEH
860 870 880 890 900
PNGILIGYTL RYVPFNGTKL GKQMVENFSP NQTKFSVQRA DPVSRYRFSL
910 920 930 940 950
SARTQVGSGE AATEESPTPP NEATPTAAPP TLPPTTVGTT GLVSSTDATA
960 970 980 990 1000
LAATSEATTV PIIPTVVPTT VATTIATTTT TTAAATTTTT TESPPTTTTG
1010 1020 1030 1040 1050
TKIHETAPDE QSIWNVTVLP NSKWANITWK HNFRPGTDFV VEYIDSNHTK
1060 1070 1080 1090 1100
KTVPVKAQAQ PIQLTDLFPG MTYTLRVYSR DNEGISSTVI TFMTSTAYTN
1110 1120 1130 1140 1150
NQTDIATQGW FIGLMCAIAL LVLILLIVCF IKRSRGGKYP VREKKDVPLG
1160 1170 1180 1190 1200
PEDPKEEDGS FDYSDEDNKP LQGSQTSLDG TIKQQESDDS LVDYGEGGEG
1210 1220 1230 1240
QFNEDGSFIG QYTVRKDKEE TEGNESSEAT SPVNAIYSLA
Length:1,240
Mass (Da):138,004
Last modified:November 14, 2003 - v2
Checksum:i636A187BC3772513
GO
Isoform 2 (identifier: P97685-2) [UniParc]FASTAAdd to basket
Also known as: NF155, 155 kDa isoform

The sequence of this isoform differs from the canonical sequence as follows:
     31-36: Missing.
     236-236: T → NNPYNDSSLRNHPDIYSA
     824-824: L → YPRAAPTEVK...TKEFTTPEGV
     928-1096: Missing.

Show »
Length:1,189
Mass (Da):133,776
Checksum:iC63224BE3EE83B1B
GO
Isoform 3 (identifier: P97685-3) [UniParc]FASTAAdd to basket
Also known as: NF155, 155 kDa isoform

The sequence of this isoform differs from the canonical sequence as follows:
     31-36: Missing.
     236-236: T → NNPYNDSSLRNHPDIYSA
     611-625: Missing.
     824-824: L → YPRAAPTEVK...TKEFTTPEGV
     928-1096: Missing.

Show »
Length:1,174
Mass (Da):132,228
Checksum:iB9FF83D9A2C20F30
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti482E → Q in AAB47753 (PubMed:8947556).Curated1
Sequence conflicti482E → Q in AAB47754 (PubMed:8947556).Curated1
Sequence conflicti675W → L in AAB47753 (PubMed:8947556).Curated1
Sequence conflicti675W → L in AAB47754 (PubMed:8947556).Curated1
Sequence conflicti699Q → D in AAB47753 (PubMed:8947556).Curated1
Sequence conflicti699Q → D in AAB47754 (PubMed:8947556).Curated1
Sequence conflicti763Y → YY in AAB47753 (PubMed:8947556).Curated1
Sequence conflicti763Y → YY in AAB47754 (PubMed:8947556).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05041631 – 36Missing in isoform 2 and isoform 3. 2 Publications6
Alternative sequenceiVSP_050417236T → NNPYNDSSLRNHPDIYSA in isoform 2 and isoform 3. 2 Publications1
Alternative sequenceiVSP_008941611 – 625Missing in isoform 3. 2 PublicationsAdd BLAST15
Alternative sequenceiVSP_050418824L → YPRAAPTEVKIRVLNSTAIS LQWNRVYPDTVQGQLREYRA YYWRESSLLKNLWVSQKRQQ ASFPGDRPRGVVGRLFPYSN YKLEMVVVNGRGDGPRSETK EFTTPEGV in isoform 2 and isoform 3. 2 Publications1
Alternative sequenceiVSP_050419928 – 1096Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST169

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061639 mRNA. Translation: AAL27854.1.
U81035 mRNA. Translation: AAB47753.1.
U81036 mRNA. Translation: AAB47754.1.
RefSeqiNP_001153785.1. NM_001160313.1. [P97685-2]
NP_001153786.1. NM_001160314.1. [P97685-1]
NP_446361.1. NM_053909.2. [P97685-3]
UniGeneiRn.3048.

Genome annotation databases

EnsembliENSRNOT00000060871; ENSRNOP00000057594; ENSRNOG00000030515. [P97685-1]
GeneIDi116690.
KEGGirno:116690.
UCSCiRGD:620911. rat. [P97685-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061639 mRNA. Translation: AAL27854.1.
U81035 mRNA. Translation: AAB47753.1.
U81036 mRNA. Translation: AAB47754.1.
RefSeqiNP_001153785.1. NM_001160313.1. [P97685-2]
NP_001153786.1. NM_001160314.1. [P97685-1]
NP_446361.1. NM_053909.2. [P97685-3]
UniGeneiRn.3048.

3D structure databases

ProteinModelPortaliP97685.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250572. 2 interactors.
MINTiMINT-205368.
STRINGi10116.ENSRNOP00000057594.

PTM databases

iPTMnetiP97685.
PhosphoSitePlusiP97685.

Proteomic databases

PaxDbiP97685.
PRIDEiP97685.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000060871; ENSRNOP00000057594; ENSRNOG00000030515. [P97685-1]
GeneIDi116690.
KEGGirno:116690.
UCSCiRGD:620911. rat. [P97685-1]

Organism-specific databases

CTDi23114.
RGDi620911. Nfasc.

Phylogenomic databases

eggNOGiKOG3513. Eukaryota.
ENOG410XSVG. LUCA.
GeneTreeiENSGT00760000118840.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiP97685.
KOiK06757.
OrthoDBiEOG091G00LY.
PhylomeDBiP97685.
TreeFamiTF351098.

Enzyme and pathway databases

ReactomeiR-RNO-445095. Interaction between L1 and Ankyrins.
R-RNO-447043. Neurofascin interactions.
R-RNO-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP97685.

Gene expression databases

ExpressionAtlasiP97685. baseline and differential.
GenevisibleiP97685. RN.

Family and domain databases

CDDicd00063. FN3. 4 hits.
Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013151. Immunoglobulin.
IPR026966. Neurofascin/L1/NrCAM_C.
IPR026965. NFASC.
[Graphical view]
PANTHERiPTHR10489:SF715. PTHR10489:SF715. 3 hits.
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 2 hits.
PF00047. ig. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
SM00409. IG. 6 hits.
SM00408. IGc2. 6 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 6 hits.
SSF49265. SSF49265. 3 hits.
PROSITEiPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNFASC_RAT
AccessioniPrimary (citable) accession number: P97685
Secondary accession number(s): P97684, Q91Z60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.