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Protein

Calcium-activated potassium channel subunit beta-1

Gene

Kcnmb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases the apparent Ca2+/voltage sensitivity of the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and alters its pharmacological properties. It slows down the activation and the deactivation kinetics of the channel. Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1. Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2). Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate (By similarity).By similarity

GO - Molecular functioni

  1. calcium-activated potassium channel activity Source: RGD
  2. potassium channel regulator activity Source: RGD

GO - Biological processi

  1. detection of calcium ion Source: GO_Central
  2. potassium ion transmembrane transport Source: GOC
  3. response to calcium ion Source: RGD
  4. synaptic transmission Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_291627. Ca2+ activated K+ channels.
REACT_349609. cGMP effects.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-activated potassium channel subunit beta-1
Alternative name(s):
BK channel subunit beta-1
Short name:
BKbeta
Short name:
BKbeta1
Calcium-activated potassium channel, subfamily M subunit beta-1
Short name:
Calcium-activated potassium channel subunit beta
Charybdotoxin receptor subunit beta-1
K(VCA)beta-1
Maxi K channel subunit beta-1
Slo-beta-1
Short name:
Slo-beta
Slowpoke-beta
Gene namesi
Name:Kcnmb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2961. Kcnmb1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1817CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei19 – 3921Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini40 – 155116ExtracellularSequence AnalysisAdd
BLAST
Transmembranei156 – 17621Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini177 – 19115CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. voltage-gated potassium channel complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 191190Calcium-activated potassium channel subunit beta-1PRO_0000187049Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Weakly expressed. In brain, it is expressed in a few discrete populations of neurons that also express KCNMA1.1 Publication

Gene expression databases

GenevestigatoriP97678.

Interactioni

Subunit structurei

Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB1 per KCNMA1 tetramer (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007408.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG40171.
GeneTreeiENSGT00390000015997.
HOGENOMiHOG000290180.
HOVERGENiHBG052223.
InParanoidiP97678.
KOiK04937.
OMAiDPGREET.
OrthoDBiEOG77WWDC.
PhylomeDBiP97678.
TreeFamiTF328589.

Family and domain databases

InterProiIPR003930. K_chnl_Ca-activ_BK_bsu.
[Graphical view]
PANTHERiPTHR10258. PTHR10258. 1 hit.
PfamiPF03185. CaKB. 1 hit.
[Graphical view]
PRINTSiPR01450. BKCHANNELB.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97678-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKKLVMAQK RGETRALCLG VAMVVCAAIT YYILGTTVLP LYQKSVWTQE
60 70 80 90 100
STCHLVETNI KDQEELEGRK VPQYPCLWVN VSAVGRWAML YHTEDTRDQN
110 120 130 140 150
QQCSYIPRNL DNYQTALVDV KKVRANFYKH HNFYCFSAPQ VNETSVVYQR
160 170 180 190
LYGPQILLFS FFWPTFLLTG GLLIIAMVKL NRSLSVLAAQ K
Length:191
Mass (Da):21,909
Last modified:January 23, 2007 - v4
Checksum:iD39F2FE4D69D7662
GO
Isoform 2 (identifier: P97678-2) [UniParc]FASTAAdd to basket

Also known as: 1b

The sequence of this isoform differs from the canonical sequence as follows:
     123-146: VRANFYKHHNFYCFSAPQVNETSV → LRSRPISSAQQHGVTRNGRGPGQA
     147-191: Missing.

Show »
Length:146
Mass (Da):16,470
Checksum:i6EF4D5EA24D68E57
GO
Isoform 3 (identifier: P97678-3) [UniParc]FASTAAdd to basket

Also known as: 1c

The sequence of this isoform differs from the canonical sequence as follows:
     103-122: Missing.
     123-146: VRANFYKHHNFYCFSAPQVNETSV → LRSRPISSAQQHGVTRNGRGPGQA
     147-191: Missing.

Show »
Length:126
Mass (Da):14,147
Checksum:i8B319BB95CE4E8CC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141T → A in AAB38413 (PubMed:9888999).Curated
Sequence conflicti28 – 303AIT → VVA in AAB38413 (PubMed:9888999).Curated
Sequence conflicti38 – 381V → M in AAB38413 (PubMed:9888999).Curated
Sequence conflicti56 – 616VETNIK → IESNIR in AAB38413 (PubMed:9888999).Curated
Sequence conflicti69 – 691R → K in AAB38413 (PubMed:9888999).Curated
Sequence conflicti89 – 891M → V in AAB38413 (PubMed:9888999).Curated
Sequence conflicti105 – 1051Y → H in AAD11548 (PubMed:9105668).Curated
Sequence conflicti109 – 1113NLD → SLE in AAB38413 (PubMed:9888999).Curated
Sequence conflicti115 – 1217TALVDVK → VARADVE in AAB38413 (PubMed:9888999).Curated
Sequence conflicti125 – 1251A → T in AAB38413 (PubMed:9888999).Curated
Sequence conflicti128 – 1325YKHHN → HEHRI in AAB38413 (PubMed:9888999).Curated
Sequence conflicti138 – 1414APQV → TTRE in AAB38413 (PubMed:9888999).Curated
Sequence conflicti145 – 1495SVVYQ → TVLYR in AAB38413 (PubMed:9888999).Curated
Sequence conflicti156 – 1561I → T in AAB38413 (PubMed:9888999).Curated
Sequence conflicti161 – 1611F → L in AAB38413 (PubMed:9888999).Curated
Sequence conflicti177 – 1771M → I in AAD11548 (PubMed:9105668).Curated
Sequence conflicti180 – 1823LNR → INQ in AAB38413 (PubMed:9888999).Curated
Sequence conflicti186 – 1872VL → IP in AAD11548 (PubMed:9105668).Curated
Sequence conflicti186 – 1861V → I in AAB38413 (PubMed:9888999).Curated
Sequence conflicti191 – 1911K → R (PubMed:9105668).Curated
Sequence conflicti191 – 1911K → R (PubMed:9888999).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei103 – 12220Missing in isoform 3. 1 PublicationVSP_009824Add
BLAST
Alternative sequencei123 – 14624VRANF…NETSV → LRSRPISSAQQHGVTRNGRG PGQA in isoform 2 and isoform 3. 1 PublicationVSP_009825Add
BLAST
Alternative sequencei147 – 19145Missing in isoform 2 and isoform 3. 1 PublicationVSP_009826Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54498 mRNA. Translation: AAD11548.1.
AF020712 mRNA. Translation: AAD11858.1.
U79661 mRNA. Translation: AAB38413.1.
U40602 mRNA. Translation: AAB96355.1.
AB010963 mRNA. Translation: BAA33448.1.
AB050745 mRNA. Translation: BAB17678.1.
RefSeqiNP_062146.1. NM_019273.1. [P97678-1]
XP_006246151.1. XM_006246089.2. [P97678-1]
XP_008765784.1. XM_008767562.1. [P97678-1]
XP_008765785.1. XM_008767563.1. [P97678-1]
XP_008765786.1. XM_008767564.1. [P97678-2]
UniGeneiRn.10820.
Rn.127785.

Genome annotation databases

EnsembliENSRNOT00000007393; ENSRNOP00000007393; ENSRNOG00000005465. [P97678-2]
ENSRNOT00000007408; ENSRNOP00000007408; ENSRNOG00000005465. [P97678-1]
GeneIDi29747.
KEGGirno:29747.
rno:60591.
UCSCiRGD:2961. rat. [P97678-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54498 mRNA. Translation: AAD11548.1.
AF020712 mRNA. Translation: AAD11858.1.
U79661 mRNA. Translation: AAB38413.1.
U40602 mRNA. Translation: AAB96355.1.
AB010963 mRNA. Translation: BAA33448.1.
AB050745 mRNA. Translation: BAB17678.1.
RefSeqiNP_062146.1. NM_019273.1. [P97678-1]
XP_006246151.1. XM_006246089.2. [P97678-1]
XP_008765784.1. XM_008767562.1. [P97678-1]
XP_008765785.1. XM_008767563.1. [P97678-1]
XP_008765786.1. XM_008767564.1. [P97678-2]
UniGeneiRn.10820.
Rn.127785.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007408.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007393; ENSRNOP00000007393; ENSRNOG00000005465. [P97678-2]
ENSRNOT00000007408; ENSRNOP00000007408; ENSRNOG00000005465. [P97678-1]
GeneIDi29747.
KEGGirno:29747.
rno:60591.
UCSCiRGD:2961. rat. [P97678-1]

Organism-specific databases

CTDi3779.
RGDi2961. Kcnmb1.

Phylogenomic databases

eggNOGiNOG40171.
GeneTreeiENSGT00390000015997.
HOGENOMiHOG000290180.
HOVERGENiHBG052223.
InParanoidiP97678.
KOiK04937.
OMAiDPGREET.
OrthoDBiEOG77WWDC.
PhylomeDBiP97678.
TreeFamiTF328589.

Enzyme and pathway databases

ReactomeiREACT_291627. Ca2+ activated K+ channels.
REACT_349609. cGMP effects.

Miscellaneous databases

NextBioi610265.
PROiP97678.

Gene expression databases

GenevestigatoriP97678.

Family and domain databases

InterProiIPR003930. K_chnl_Ca-activ_BK_bsu.
[Graphical view]
PANTHERiPTHR10258. PTHR10258. 1 hit.
PfamiPF03185. CaKB. 1 hit.
[Graphical view]
PRINTSiPR01450. BKCHANNELB.
ProtoNetiSearch...

Publicationsi

  1. "Differential expression of the alpha and beta subunits of the large-conductance calcium-activated potassium channel: implication for channel diversity."
    Chang C.-P., Dworetzky S.I., Wang J., Goldstein M.E.
    Brain Res. Mol. Brain Res. 45:33-40(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
  2. "Human and rodent MaxiK channel beta-subunit genes: cloning and characterization."
    Jiang Z., Wallner M., Meera P., Toro L.
    Genomics 55:57-67(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Uterus.
  3. Lange A.R., Gebremedhin D., Aebly M., Harder D.R.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Vascular smooth muscle.
  4. Reimann F.
    Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Uterus.
  5. "Molecular cloning of a novel spliced variant of calcium activated potassium channel beta subunit in rat smooth muscle."
    Ohya S., Watanabe M., Imaizumi Y.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
    Tissue: Aorta.

Entry informationi

Entry nameiKCMB1_RAT
AccessioniPrimary (citable) accession number: P97678
Secondary accession number(s): O35337
, O88805, Q9ESK7, Q9QWI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: April 29, 2015
This is version 101 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.