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P97678

- KCMB1_RAT

UniProt

P97678 - KCMB1_RAT

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Protein
Calcium-activated potassium channel subunit beta-1
Gene
Kcnmb1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases the apparent Ca2+/voltage sensitivity of the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and alters its pharmacological properties. It slows down the activation and the deactivation kinetics of the channel. Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1. Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2). Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate By similarity.

GO - Molecular functioni

  1. calcium-activated potassium channel activity Source: RGD
  2. potassium channel regulator activity Source: RGD
Complete GO annotation...

GO - Biological processi

  1. potassium ion transmembrane transport Source: GOC
  2. response to calcium ion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_199213. Ca2+ activated K+ channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-activated potassium channel subunit beta-1
Alternative name(s):
BK channel subunit beta-1
Short name:
BKbeta
Short name:
BKbeta1
Calcium-activated potassium channel, subfamily M subunit beta-1
Short name:
Calcium-activated potassium channel subunit beta
Charybdotoxin receptor subunit beta-1
K(VCA)beta-1
Maxi K channel subunit beta-1
Slo-beta-1
Short name:
Slo-beta
Slowpoke-beta
Gene namesi
Name:Kcnmb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi2961. Kcnmb1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1817Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei19 – 3921Helical; Name=1; Reviewed prediction
Add
BLAST
Topological domaini40 – 155116Extracellular Reviewed prediction
Add
BLAST
Transmembranei156 – 17621Helical; Name=2; Reviewed prediction
Add
BLAST
Topological domaini177 – 19115Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. voltage-gated potassium channel complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 191190Calcium-activated potassium channel subunit beta-1
PRO_0000187049Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi80 – 801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi142 – 1421N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated By similarity.

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Weakly expressed. In brain, it is expressed in a few discrete populations of neurons that also express KCNMA1.1 Publication

Gene expression databases

GenevestigatoriP97678.

Interactioni

Subunit structurei

Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB1 per KCNMA1 tetramer By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007408.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG40171.
GeneTreeiENSGT00390000015997.
HOGENOMiHOG000290180.
HOVERGENiHBG052223.
KOiK04937.
OMAiIIAMVKI.
OrthoDBiEOG77WWDC.
PhylomeDBiP97678.
TreeFamiTF328589.

Family and domain databases

InterProiIPR003930. K_chnl_Ca-activ_BK_bsu.
[Graphical view]
PANTHERiPTHR10258. PTHR10258. 1 hit.
PfamiPF03185. CaKB. 1 hit.
[Graphical view]
PRINTSiPR01450. BKCHANNELB.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P97678-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGKKLVMAQK RGETRALCLG VAMVVCAAIT YYILGTTVLP LYQKSVWTQE    50
STCHLVETNI KDQEELEGRK VPQYPCLWVN VSAVGRWAML YHTEDTRDQN 100
QQCSYIPRNL DNYQTALVDV KKVRANFYKH HNFYCFSAPQ VNETSVVYQR 150
LYGPQILLFS FFWPTFLLTG GLLIIAMVKL NRSLSVLAAQ K 191
Length:191
Mass (Da):21,909
Last modified:January 23, 2007 - v4
Checksum:iD39F2FE4D69D7662
GO
Isoform 2 (identifier: P97678-2) [UniParc]FASTAAdd to Basket

Also known as: 1b

The sequence of this isoform differs from the canonical sequence as follows:
     123-146: VRANFYKHHNFYCFSAPQVNETSV → LRSRPISSAQQHGVTRNGRGPGQA
     147-191: Missing.

Show »
Length:146
Mass (Da):16,470
Checksum:i6EF4D5EA24D68E57
GO
Isoform 3 (identifier: P97678-3) [UniParc]FASTAAdd to Basket

Also known as: 1c

The sequence of this isoform differs from the canonical sequence as follows:
     103-122: Missing.
     123-146: VRANFYKHHNFYCFSAPQVNETSV → LRSRPISSAQQHGVTRNGRGPGQA
     147-191: Missing.

Show »
Length:126
Mass (Da):14,147
Checksum:i8B319BB95CE4E8CC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei103 – 12220Missing in isoform 3.
VSP_009824Add
BLAST
Alternative sequencei123 – 14624VRANF…NETSV → LRSRPISSAQQHGVTRNGRG PGQA in isoform 2 and isoform 3.
VSP_009825Add
BLAST
Alternative sequencei147 – 19145Missing in isoform 2 and isoform 3.
VSP_009826Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141T → A in AAB38413. 1 Publication
Sequence conflicti28 – 303AIT → VVA in AAB38413. 1 Publication
Sequence conflicti38 – 381V → M in AAB38413. 1 Publication
Sequence conflicti56 – 616VETNIK → IESNIR in AAB38413. 1 Publication
Sequence conflicti69 – 691R → K in AAB38413. 1 Publication
Sequence conflicti89 – 891M → V in AAB38413. 1 Publication
Sequence conflicti105 – 1051Y → H in AAD11548. 1 Publication
Sequence conflicti109 – 1113NLD → SLE in AAB38413. 1 Publication
Sequence conflicti115 – 1217TALVDVK → VARADVE in AAB38413. 1 Publication
Sequence conflicti125 – 1251A → T in AAB38413. 1 Publication
Sequence conflicti128 – 1325YKHHN → HEHRI in AAB38413. 1 Publication
Sequence conflicti138 – 1414APQV → TTRE in AAB38413. 1 Publication
Sequence conflicti145 – 1495SVVYQ → TVLYR in AAB38413. 1 Publication
Sequence conflicti156 – 1561I → T in AAB38413. 1 Publication
Sequence conflicti161 – 1611F → L in AAB38413. 1 Publication
Sequence conflicti177 – 1771M → I in AAD11548. 1 Publication
Sequence conflicti180 – 1823LNR → INQ in AAB38413. 1 Publication
Sequence conflicti186 – 1872VL → IP in AAD11548. 1 Publication
Sequence conflicti186 – 1861V → I in AAB38413. 1 Publication
Sequence conflicti191 – 1911K → R1 Publication
Sequence conflicti191 – 1911K → R1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U54498 mRNA. Translation: AAD11548.1.
AF020712 mRNA. Translation: AAD11858.1.
U79661 mRNA. Translation: AAB38413.1.
U40602 mRNA. Translation: AAB96355.1.
AB010963 mRNA. Translation: BAA33448.1.
AB050745 mRNA. Translation: BAB17678.1.
RefSeqiNP_062146.1. NM_019273.1. [P97678-1]
NP_071539.1. NM_022203.1.
XP_006246151.1. XM_006246089.1. [P97678-1]
XP_006246152.1. XM_006246090.1. [P97678-1]
XP_006246153.1. XM_006246091.1. [P97678-2]
UniGeneiRn.10820.
Rn.127785.

Genome annotation databases

EnsembliENSRNOT00000007393; ENSRNOP00000007393; ENSRNOG00000005465. [P97678-2]
ENSRNOT00000007408; ENSRNOP00000007408; ENSRNOG00000005465. [P97678-1]
GeneIDi29747.
60591.
KEGGirno:29747.
rno:60591.
UCSCiRGD:2961. rat. [P97678-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U54498 mRNA. Translation: AAD11548.1 .
AF020712 mRNA. Translation: AAD11858.1 .
U79661 mRNA. Translation: AAB38413.1 .
U40602 mRNA. Translation: AAB96355.1 .
AB010963 mRNA. Translation: BAA33448.1 .
AB050745 mRNA. Translation: BAB17678.1 .
RefSeqi NP_062146.1. NM_019273.1. [P97678-1 ]
NP_071539.1. NM_022203.1.
XP_006246151.1. XM_006246089.1. [P97678-1 ]
XP_006246152.1. XM_006246090.1. [P97678-1 ]
XP_006246153.1. XM_006246091.1. [P97678-2 ]
UniGenei Rn.10820.
Rn.127785.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000007408.

Chemistry

BindingDBi P97678.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000007393 ; ENSRNOP00000007393 ; ENSRNOG00000005465 . [P97678-2 ]
ENSRNOT00000007408 ; ENSRNOP00000007408 ; ENSRNOG00000005465 . [P97678-1 ]
GeneIDi 29747.
60591.
KEGGi rno:29747.
rno:60591.
UCSCi RGD:2961. rat. [P97678-1 ]

Organism-specific databases

CTDi 3779.
RGDi 2961. Kcnmb1.

Phylogenomic databases

eggNOGi NOG40171.
GeneTreei ENSGT00390000015997.
HOGENOMi HOG000290180.
HOVERGENi HBG052223.
KOi K04937.
OMAi IIAMVKI.
OrthoDBi EOG77WWDC.
PhylomeDBi P97678.
TreeFami TF328589.

Enzyme and pathway databases

Reactomei REACT_199213. Ca2+ activated K+ channels.

Miscellaneous databases

NextBioi 610265.
PROi P97678.

Gene expression databases

Genevestigatori P97678.

Family and domain databases

InterProi IPR003930. K_chnl_Ca-activ_BK_bsu.
[Graphical view ]
PANTHERi PTHR10258. PTHR10258. 1 hit.
Pfami PF03185. CaKB. 1 hit.
[Graphical view ]
PRINTSi PR01450. BKCHANNELB.
ProtoNeti Search...

Publicationsi

  1. "Differential expression of the alpha and beta subunits of the large-conductance calcium-activated potassium channel: implication for channel diversity."
    Chang C.-P., Dworetzky S.I., Wang J., Goldstein M.E.
    Brain Res. Mol. Brain Res. 45:33-40(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
  2. "Human and rodent MaxiK channel beta-subunit genes: cloning and characterization."
    Jiang Z., Wallner M., Meera P., Toro L.
    Genomics 55:57-67(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Uterus.
  3. Lange A.R., Gebremedhin D., Aebly M., Harder D.R.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Vascular smooth muscle.
  4. Reimann F.
    Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Uterus.
  5. "Molecular cloning of a novel spliced variant of calcium activated potassium channel beta subunit in rat smooth muscle."
    Ohya S., Watanabe M., Imaizumi Y.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
    Tissue: Aorta.

Entry informationi

Entry nameiKCMB1_RAT
AccessioniPrimary (citable) accession number: P97678
Secondary accession number(s): O35337
, O88805, Q9ESK7, Q9QWI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 93 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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