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P97678

- KCMB1_RAT

UniProt

P97678 - KCMB1_RAT

Protein

Calcium-activated potassium channel subunit beta-1

Gene

Kcnmb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases the apparent Ca2+/voltage sensitivity of the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and alters its pharmacological properties. It slows down the activation and the deactivation kinetics of the channel. Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1. Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2). Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate By similarity.By similarity

    GO - Molecular functioni

    1. calcium-activated potassium channel activity Source: RGD
    2. potassium channel regulator activity Source: RGD

    GO - Biological processi

    1. potassium ion transmembrane transport Source: GOC
    2. response to calcium ion Source: RGD

    Keywords - Molecular functioni

    Ion channel

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_199213. Ca2+ activated K+ channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium-activated potassium channel subunit beta-1
    Alternative name(s):
    BK channel subunit beta-1
    Short name:
    BKbeta
    Short name:
    BKbeta1
    Calcium-activated potassium channel, subfamily M subunit beta-1
    Short name:
    Calcium-activated potassium channel subunit beta
    Charybdotoxin receptor subunit beta-1
    K(VCA)beta-1
    Maxi K channel subunit beta-1
    Slo-beta-1
    Short name:
    Slo-beta
    Slowpoke-beta
    Gene namesi
    Name:Kcnmb1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi2961. Kcnmb1.

    Subcellular locationi

    GO - Cellular componenti

    1. voltage-gated potassium channel complex Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 191190Calcium-activated potassium channel subunit beta-1PRO_0000187049Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Weakly expressed. In brain, it is expressed in a few discrete populations of neurons that also express KCNMA1.1 Publication

    Gene expression databases

    GenevestigatoriP97678.

    Interactioni

    Subunit structurei

    Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB1 per KCNMA1 tetramer By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000007408.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 1817CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini40 – 155116ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini177 – 19115CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei19 – 3921Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei156 – 17621Helical; Name=2Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG40171.
    GeneTreeiENSGT00390000015997.
    HOGENOMiHOG000290180.
    HOVERGENiHBG052223.
    KOiK04937.
    OMAiIIAMVKI.
    OrthoDBiEOG77WWDC.
    PhylomeDBiP97678.
    TreeFamiTF328589.

    Family and domain databases

    InterProiIPR003930. K_chnl_Ca-activ_BK_bsu.
    [Graphical view]
    PANTHERiPTHR10258. PTHR10258. 1 hit.
    PfamiPF03185. CaKB. 1 hit.
    [Graphical view]
    PRINTSiPR01450. BKCHANNELB.

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P97678-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGKKLVMAQK RGETRALCLG VAMVVCAAIT YYILGTTVLP LYQKSVWTQE    50
    STCHLVETNI KDQEELEGRK VPQYPCLWVN VSAVGRWAML YHTEDTRDQN 100
    QQCSYIPRNL DNYQTALVDV KKVRANFYKH HNFYCFSAPQ VNETSVVYQR 150
    LYGPQILLFS FFWPTFLLTG GLLIIAMVKL NRSLSVLAAQ K 191
    Length:191
    Mass (Da):21,909
    Last modified:January 23, 2007 - v4
    Checksum:iD39F2FE4D69D7662
    GO
    Isoform 2 (identifier: P97678-2) [UniParc]FASTAAdd to Basket

    Also known as: 1b

    The sequence of this isoform differs from the canonical sequence as follows:
         123-146: VRANFYKHHNFYCFSAPQVNETSV → LRSRPISSAQQHGVTRNGRGPGQA
         147-191: Missing.

    Show »
    Length:146
    Mass (Da):16,470
    Checksum:i6EF4D5EA24D68E57
    GO
    Isoform 3 (identifier: P97678-3) [UniParc]FASTAAdd to Basket

    Also known as: 1c

    The sequence of this isoform differs from the canonical sequence as follows:
         103-122: Missing.
         123-146: VRANFYKHHNFYCFSAPQVNETSV → LRSRPISSAQQHGVTRNGRGPGQA
         147-191: Missing.

    Show »
    Length:126
    Mass (Da):14,147
    Checksum:i8B319BB95CE4E8CC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141T → A in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti28 – 303AIT → VVA in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti38 – 381V → M in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti56 – 616VETNIK → IESNIR in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti69 – 691R → K in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti89 – 891M → V in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti105 – 1051Y → H in AAD11548. (PubMed:9105668)Curated
    Sequence conflicti109 – 1113NLD → SLE in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti115 – 1217TALVDVK → VARADVE in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti125 – 1251A → T in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti128 – 1325YKHHN → HEHRI in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti138 – 1414APQV → TTRE in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti145 – 1495SVVYQ → TVLYR in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti156 – 1561I → T in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti161 – 1611F → L in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti177 – 1771M → I in AAD11548. (PubMed:9105668)Curated
    Sequence conflicti180 – 1823LNR → INQ in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti186 – 1872VL → IP in AAD11548. (PubMed:9105668)Curated
    Sequence conflicti186 – 1861V → I in AAB38413. (PubMed:9888999)Curated
    Sequence conflicti191 – 1911K → R(PubMed:9105668)Curated
    Sequence conflicti191 – 1911K → R(PubMed:9888999)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei103 – 12220Missing in isoform 3. 1 PublicationVSP_009824Add
    BLAST
    Alternative sequencei123 – 14624VRANF…NETSV → LRSRPISSAQQHGVTRNGRG PGQA in isoform 2 and isoform 3. 1 PublicationVSP_009825Add
    BLAST
    Alternative sequencei147 – 19145Missing in isoform 2 and isoform 3. 1 PublicationVSP_009826Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U54498 mRNA. Translation: AAD11548.1.
    AF020712 mRNA. Translation: AAD11858.1.
    U79661 mRNA. Translation: AAB38413.1.
    U40602 mRNA. Translation: AAB96355.1.
    AB010963 mRNA. Translation: BAA33448.1.
    AB050745 mRNA. Translation: BAB17678.1.
    RefSeqiNP_062146.1. NM_019273.1. [P97678-1]
    NP_071539.1. NM_022203.1.
    XP_006246151.1. XM_006246089.1. [P97678-1]
    XP_006246152.1. XM_006246090.1. [P97678-1]
    XP_006246153.1. XM_006246091.1. [P97678-2]
    UniGeneiRn.10820.
    Rn.127785.

    Genome annotation databases

    EnsembliENSRNOT00000007393; ENSRNOP00000007393; ENSRNOG00000005465. [P97678-2]
    ENSRNOT00000007408; ENSRNOP00000007408; ENSRNOG00000005465. [P97678-1]
    GeneIDi29747.
    60591.
    KEGGirno:29747.
    rno:60591.
    UCSCiRGD:2961. rat. [P97678-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U54498 mRNA. Translation: AAD11548.1 .
    AF020712 mRNA. Translation: AAD11858.1 .
    U79661 mRNA. Translation: AAB38413.1 .
    U40602 mRNA. Translation: AAB96355.1 .
    AB010963 mRNA. Translation: BAA33448.1 .
    AB050745 mRNA. Translation: BAB17678.1 .
    RefSeqi NP_062146.1. NM_019273.1. [P97678-1 ]
    NP_071539.1. NM_022203.1.
    XP_006246151.1. XM_006246089.1. [P97678-1 ]
    XP_006246152.1. XM_006246090.1. [P97678-1 ]
    XP_006246153.1. XM_006246091.1. [P97678-2 ]
    UniGenei Rn.10820.
    Rn.127785.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000007408.

    Chemistry

    BindingDBi P97678.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000007393 ; ENSRNOP00000007393 ; ENSRNOG00000005465 . [P97678-2 ]
    ENSRNOT00000007408 ; ENSRNOP00000007408 ; ENSRNOG00000005465 . [P97678-1 ]
    GeneIDi 29747.
    60591.
    KEGGi rno:29747.
    rno:60591.
    UCSCi RGD:2961. rat. [P97678-1 ]

    Organism-specific databases

    CTDi 3779.
    RGDi 2961. Kcnmb1.

    Phylogenomic databases

    eggNOGi NOG40171.
    GeneTreei ENSGT00390000015997.
    HOGENOMi HOG000290180.
    HOVERGENi HBG052223.
    KOi K04937.
    OMAi IIAMVKI.
    OrthoDBi EOG77WWDC.
    PhylomeDBi P97678.
    TreeFami TF328589.

    Enzyme and pathway databases

    Reactomei REACT_199213. Ca2+ activated K+ channels.

    Miscellaneous databases

    NextBioi 610265.
    PROi P97678.

    Gene expression databases

    Genevestigatori P97678.

    Family and domain databases

    InterProi IPR003930. K_chnl_Ca-activ_BK_bsu.
    [Graphical view ]
    PANTHERi PTHR10258. PTHR10258. 1 hit.
    Pfami PF03185. CaKB. 1 hit.
    [Graphical view ]
    PRINTSi PR01450. BKCHANNELB.
    ProtoNeti Search...

    Publicationsi

    1. "Differential expression of the alpha and beta subunits of the large-conductance calcium-activated potassium channel: implication for channel diversity."
      Chang C.-P., Dworetzky S.I., Wang J., Goldstein M.E.
      Brain Res. Mol. Brain Res. 45:33-40(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
    2. "Human and rodent MaxiK channel beta-subunit genes: cloning and characterization."
      Jiang Z., Wallner M., Meera P., Toro L.
      Genomics 55:57-67(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: Sprague-Dawley.
      Tissue: Uterus.
    3. Lange A.R., Gebremedhin D., Aebly M., Harder D.R.
      Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: Sprague-Dawley.
      Tissue: Vascular smooth muscle.
    4. Reimann F.
      Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: Sprague-Dawley.
      Tissue: Uterus.
    5. "Molecular cloning of a novel spliced variant of calcium activated potassium channel beta subunit in rat smooth muscle."
      Ohya S., Watanabe M., Imaizumi Y.
      Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
      Tissue: Aorta.

    Entry informationi

    Entry nameiKCMB1_RAT
    AccessioniPrimary (citable) accession number: P97678
    Secondary accession number(s): O35337
    , O88805, Q9ESK7, Q9QWI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 94 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3