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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase family member 3

Gene

Enpp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.By similarity

Catalytic activityi

Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.By similarity
A nucleoside triphosphate + H2O = a nucleotide + diphosphate.By similarity

Cofactori

a divalent metal cationCuratedNote: Binds 2 divalent metal cations per subunit.Curated

Enzyme regulationi

At low concentrations of ATP, a phosphorylated active site intermediate can be formed which inhibits further hydrolysis.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi168Zinc 1; catalyticBy similarity1
Active sitei206AMP-threonine intermediateBy similarity1
Metal bindingi206Zinc 1; catalyticBy similarity1
Binding sitei227SubstrateBy similarity1
Binding sitei321SubstrateBy similarity1
Metal bindingi326Zinc 2; catalyticBy similarity1
Metal bindingi330Zinc 2; via tele nitrogen; catalyticBy similarity1
Metal bindingi373Zinc 1; catalyticBy similarity1
Metal bindingi374Zinc 1; via tele nitrogen; catalyticBy similarity1
Metal bindingi483Zinc 2; via tele nitrogen; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.6.1.9 5301
SABIO-RKP97675

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
Short name:
E-NPP 3
Alternative name(s):
B10
Phosphodiesterase I beta
Short name:
PD-Ibeta
Phosphodiesterase I/nucleotide pyrophosphatase 3
RB13-6 antigen
CD_antigen: CD203c
Including the following 2 domains:
Alkaline phosphodiesterase I (EC:3.1.4.1By similarity)
Nucleotide pyrophosphatase (EC:3.6.1.9By similarity)
Short name:
NPPase
Alternative name(s):
Nucleotide diphosphataseCurated
Gene namesi
Name:Enpp3
Synonyms:Pdnp3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi708511 Enpp3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST19
Topological domaini31 – 875ExtracellularSequence analysisAdd BLAST845

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001885711 – 875Ectonucleotide pyrophosphatase/phosphodiesterase family member 3Add BLAST875

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi55 ↔ 72PROSITE-ProRule annotation
Disulfide bondi59 ↔ 90PROSITE-ProRule annotation
Disulfide bondi70 ↔ 83PROSITE-ProRule annotation
Disulfide bondi76 ↔ 82PROSITE-ProRule annotation
Disulfide bondi99 ↔ 116PROSITE-ProRule annotation
Disulfide bondi104 ↔ 134PROSITE-ProRule annotation
Disulfide bondi114 ↔ 127PROSITE-ProRule annotation
Disulfide bondi120 ↔ 126PROSITE-ProRule annotation
Disulfide bondi145 ↔ 191PROSITE-ProRule annotation
Disulfide bondi153 ↔ 365PROSITE-ProRule annotation
Glycosylationi237N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi280N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi289N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi381 ↔ 478PROSITE-ProRule annotation
Disulfide bondi429 ↔ 818PROSITE-ProRule annotation
Glycosylationi533N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi574N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi575 ↔ 679PROSITE-ProRule annotation
Disulfide bondi577 ↔ 664PROSITE-ProRule annotation
Glycosylationi594N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi702N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi787 ↔ 797PROSITE-ProRule annotation
Glycosylationi789N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The N-terminal is blocked.
N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal. No O-glycosylation.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP97675
PRIDEiP97675

PTM databases

iPTMnetiP97675
PhosphoSitePlusiP97675

Expressioni

Tissue specificityi

Highly expressed in intestinal epithelium. Also expressed in liver.1 Publication

Gene expression databases

BgeeiENSRNOG00000013791
GenevisibleiP97675 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018695

Structurei

3D structure databases

ProteinModelPortaliP97675
SMRiP97675
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 94SMB 1PROSITE-ProRule annotationAdd BLAST44
Domaini95 – 139SMB 2PROSITE-ProRule annotationAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni141 – 510PhosphodiesteraseAdd BLAST370
Regioni605 – 875NucleaseAdd BLAST271

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi79 – 81Cell attachment siteSequence analysis3

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2645 Eukaryota
COG1524 LUCA
GeneTreeiENSGT00760000119157
HOGENOMiHOG000037439
HOVERGENiHBG051484
InParanoidiP97675
KOiK01513
OMAiWDYFHSV
OrthoDBiEOG091G017X
PhylomeDBiP97675
TreeFamiTF330032

Family and domain databases

Gene3Di3.40.720.10, 3 hits
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR017850 Alkaline_phosphatase_core_sf
IPR001604 DNA/RNA_non-sp_Endonuclease
IPR020821 Extracellular_endonuc_su_A
IPR002591 Phosphodiest/P_Trfase
IPR036024 Somatomedin_B-like_dom_sf
IPR001212 Somatomedin_B_dom
PfamiView protein in Pfam
PF01223 Endonuclease_NS, 1 hit
PF01663 Phosphodiest, 1 hit
PF01033 Somatomedin_B, 2 hits
SMARTiView protein in SMART
SM00892 Endonuclease_NS, 1 hit
SM00477 NUC, 1 hit
SM00201 SO, 2 hits
SUPFAMiSSF53649 SSF53649, 1 hit
SSF90188 SSF90188, 2 hits
PROSITEiView protein in PROSITE
PS00524 SMB_1, 2 hits
PS50958 SMB_2, 2 hits

Sequencei

Sequence statusi: Complete.

P97675-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSRLALATE EPIKKDSLKR YKILCAVLLA LLVIVSLGLG LGLGLRKPEE
60 70 80 90 100
HIGSCRKKCF DSSHRGLEGC RCDSGCTDRG DCCWDFEDTC VKSTQIWTCN
110 120 130 140 150
SFRCGETRLE AALCSCADDC LQRKDCCTDY KAVCQGEVPW VTEACASSQE
160 170 180 190 200
PQCPEGFDQP PVILFSMDGF RAEYLQTWST LLPNINKLKT CGLHSKYMRA
210 220 230 240 250
MYPTKTFPNH YTIVTGLYPE SHGIIDNNMY DVYLNKNFSL SSVEKSNPAW
260 270 280 290 300
WSGQPIWLTA MYQGLKAASY YWPGSDVAVN GSFPNIYRNY SNSVPYESRI
310 320 330 340 350
ATLLQWLDLP KAERPSFYTI YVEEPDSAGH KSGPVSAGVI KALQLVDDAF
360 370 380 390 400
GMLMEGLKQR NLHNCVNIIV LADHGMDQTS CDRVEYMTDY FPEINFYMYQ
410 420 430 440 450
GPAPRIRTRN IPQDFFTFNS EEIVRDLSCR KSDQHFKPYL TPDLPKRLHY
460 470 480 490 500
AKNVRIDKVH LMVDRQWLAY RNKGSSNCEG GTHGYNNEFK SMEAIFLAHG
510 520 530 540 550
PSFKEKTVIE PFENIEVYNL LCDLLHIQPA PNNGSHGSLN HLLKAPFYQP
560 570 580 590 600
SHAEELSKSA GCGFTTPLPK DSLNCSCLAL QTSGQEEQVN QRLNLSGGEV
610 620 630 640 650
SATEKTNLPF GRPRVIQKNK DHCLLYHREY VSGFGKAMKM PMWSSYTVPK
660 670 680 690 700
PGDTSSLPPT VPDCLRADVR VDPSESQKCS FYLADQNIDH GFLYPPAIKG
710 720 730 740 750
NNESQYDALI TSNLVPMYKE FKKMWDYFHK VLLIKYAIER NGVNVVSGPI
760 770 780 790 800
FDYNYDGHFD APDEITNYVA GTDVPVPTHY FVVLTSCKNK THTPDSCPGW
810 820 830 840 850
LDVLPFVVPH RPTNVESCPE NKAEDLWVEE RFKAHIARVR DVELLTGLDF
860 870
YQEKTQPVSE ILQLKTYLPT FETII
Length:875
Mass (Da):99,072
Last modified:September 19, 2002 - v2
Checksum:i4205F263E8A933EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111A → T in BAA06333 (Ref. 3) Curated1
Sequence conflicti273P → L in CAA88029 (PubMed:7730366).Curated1
Sequence conflicti475 – 476SS → VP in BAA06333 (Ref. 3) Curated2
Sequence conflicti814N → KP in BAA06333 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti124K → E. 1
Natural varianti201M → V. 1
Natural varianti596 – 597SG → NR. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47987 mRNA Translation: CAA88029.1
U78787 mRNA Translation: AAB61535.1
U78788 mRNA Translation: AAB61536.1
D30649 mRNA Translation: BAA06333.1
BC097326 mRNA Translation: AAH97326.1
PIRiA57080
RefSeqiNP_062243.2, NM_019370.2
UniGeneiRn.44

Genome annotation databases

EnsembliENSRNOT00000018695; ENSRNOP00000018695; ENSRNOG00000013791
GeneIDi54410
KEGGirno:54410

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiENPP3_RAT
AccessioniPrimary (citable) accession number: P97675
Secondary accession number(s): P70641
, P97676, Q4V8L6, Q63490
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: September 19, 2002
Last modified: May 23, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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