P97636 (IL18_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-18
Short name=IL-18

Alternative name(s):
Interferon gamma-inducing factor
Short name=IFN-gamma-inducing factor
Interleukin-1 gamma
Short name=IL-1 gamma

Gene names

Name:	Il18
Synonyms:	Igif

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Subcellular location	Secreted.
Post-translational modification	The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form By similarity.
Sequence similarities	Belongs to the IL-1 family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Molecular function	Cytokine
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade Inferred from electronic annotation. Source: Compara T-helper 1 type immune response Inferred from sequence or structural similarity PubMed 8766574. Source: UniProtKB angiogenesis Inferred from sequence or structural similarity PubMed 11466388. Source: UniProtKB cellular response to cAMP Inferred from expression pattern PubMed 12490070. Source: RGD cellular response to hydrogen peroxide Inferred from expression pattern PubMed 12684057. Source: RGD cellular response to interferon-gamma Inferred from expression pattern PubMed 18547159. Source: RGD cellular response to lipopolysaccharide Inferred from expression pattern PubMed 11739527. Source: RGD cellular response to mechanical stimulus Inferred from expression pattern PubMed 18320471. Source: RGD cellular response to parathyroid hormone stimulus Inferred from expression pattern PubMed 18165223. Source: RGD cellular response to peptide hormone stimulus Inferred from mutant phenotype PubMed 12490070. Source: RGD cellular response to tumor necrosis factor Inferred from expression pattern PubMed 18547159. Source: RGD detection of mechanical stimulus involved in sensory perception of pain Inferred from direct assay PubMed 19853379. Source: RGD digestive tract morphogenesis Inferred from expression pattern PubMed 21088234. Source: RGD inflammatory response Inferred from electronic annotation. Source: Compara interferon-gamma biosynthetic process Inferred from electronic annotation. Source: Compara learning or memory Traceable author statement PubMed 12468024. Source: RGD lipopolysaccharide-mediated signaling pathway Inferred from electronic annotation. Source: Compara lung development Inferred from expression pattern PubMed 18952566. Source: RGD osteoblast differentiation Inferred from expression pattern PubMed 18165223. Source: RGD positive regulation of NK T cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of activated T cell proliferation Inferred from sequence or structural similarity PubMed 8766574. Source: UniProtKB positive regulation of blood vessel remodeling Inferred from direct assay PubMed 19717152. Source: RGD positive regulation of chemokine production Inferred from mutant phenotype PubMed 19497959. Source: RGD positive regulation of collagen biosynthetic process Inferred from direct assay PubMed 20644901. Source: RGD positive regulation of gene expression Inferred from electronic annotation. Source: Compara positive regulation of granulocyte macrophage colony-stimulating factor production Inferred from electronic annotation. Source: Compara positive regulation of interferon-gamma production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-1 beta production Inferred from expression pattern PubMed 17623049. Source: RGD positive regulation of interleukin-17 production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-8 production Inferred from direct assay PubMed 18547159. Source: RGD positive regulation of male germ cell proliferation Inferred from direct assay PubMed 16002206. Source: RGD positive regulation of natural killer cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of neuron apoptotic process Inferred from mutant phenotype PubMed 17188500. Source: RGD positive regulation of superoxide anion generation Inferred from mutant phenotype PubMed 19497959. Source: RGD positive regulation of tumor necrosis factor production Inferred from mutant phenotype PubMed 17623049. Source: RGD positive regulation of vascular permeability Inferred from mutant phenotype PubMed 11739527. Source: RGD regulation of cell adhesion Inferred from sequence or structural similarity PubMed 11477102. Source: UniProtKB regulation of circadian sleep/wake cycle, non-REM sleep Traceable author statement PubMed 11506998. Source: UniProtKB response to cold Inferred from expression pattern Ref.1. Source: RGD response to hyperoxia Inferred from expression pattern PubMed 19672068. Source: RGD response to hypoxia Inferred from mutant phenotype PubMed 12122053. Source: RGD response to radiation Inferred from expression pattern PubMed 19955748. Source: RGD sleep Inferred from direct assay PubMed 11506998. Source: UniProtKB
Cellular_component	apical plasma membrane Inferred from direct assay PubMed 12032269. Source: RGD cytoplasm Inferred from direct assay PubMed 12032269. Source: RGD extracellular space Inferred from direct assay PubMed 16002206PubMed 19717152. Source: RGD
Molecular_function	interleukin-18 receptor binding Traceable author statement PubMed 12122053. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Beta (identifier: P97636-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Alpha (identifier: P97636-2)

The sequence of this isoform differs from the canonical sequence as follows:
     121-139: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 36	36	By similarity		PRO_0000015349
	Chain	37 – 194	158	Interleukin-18		PRO_0000015350
Natural variations
	Alternative sequence	121 – 139	19	Missing in isoform Alpha.		VSP_002659
Experimental info
	Sequence conflict	4 – 5	2	MS → IP in CAA11001. Ref.2
	Sequence conflict	4 – 5	2	MS → IP in AAL77211. Ref.4
	Sequence conflict	48	1	I → M in CAA11001. Ref.2
	Sequence conflict	48	1	I → M in AAL77211. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform Beta [UniParc]. Last modified May 1, 1997. Version 1. Checksum: E2089AD6F1798450	FASTA	194	22,303
	10 20 30 40 50 60 MAAMSEEGSC VNFKEMMFID NTLYLIPEDN GDLESDHFGR LHCTTAVIRS INDQVLFVDK 70 80 90 100 110 120 RNPPVFEDMP DIDRTANESQ TRLIIYMYKD SEVRGLAVTL SVKDGRMSTL SCKNKIISFE 130 140 150 160 170 180 EMNPPENIDD IKSDLIFFQK RVPGHNKMEF ESSLYEGHFL ACQKEDDAFK LVLKRKDENG 190 DKSVMFTLTN LHQS
	Isoform Alpha [UniParc]. Checksum: 360F0E03766AED50	FASTA	175	20,056
	10 20 30 40 50 60 MAAMSEEGSC VNFKEMMFID NTLYLIPEDN GDLESDHFGR LHCTTAVIRS INDQVLFVDK 70 80 90 100 110 120 RNPPVFEDMP DIDRTANESQ TRLIIYMYKD SEVRGLAVTL SVKDGRMSTL SCKNKIISFE 130 140 150 160 170 KRVPGHNKME FESSLYEGHF LACQKEDDAF KLVLKRKDEN GDKSVMFTLT NLHQS

References

[1]	"Induction of interferon-gamma inducing factor in the adrenal cortex." Conti B., Jahng J.W., Tinti C., Son J.H., Joh T.H. J. Biol. Chem. 272:2035-2037(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA). Strain: Sprague-Dawley. Tissue: Adrenal gland.
[2]	"Cloning of rat brain interleukin-18 cDNA." Culhane A.C., Hall M.D., Rothwell N.J., Luheshi G.N. Mol. Psychiatry 3:362-366(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA). Strain: Sprague-Dawley. Tissue: Brain.
[3]	"Cloning of the cDNA for interleukin-18 in PC12 and expression in Escherichia coli." Kim S.-J., Kim C.-S., Song K.-Y., Kim J.-S., Jung K.-S. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA). Strain: New England Deaconess Hospital. Tissue: Adrenal gland.
[4]	"IL-18 translational inhibition restricts IFN-gamma expression in crescentic glomerulonephritis." Garcia G.E., Xia Y., Ku G., Johnson R.J., Wilson C.B., Feng L. Kidney Int. 64:160-169(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA). Strain: Fischer 344.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U77776 mRNA. Translation: AAC53009.1. U77777 mRNA. Translation: AAC53010.1. AJ222813 mRNA. Translation: CAA11001.1. AY258448 mRNA. Translation: AAP14669.1. AY077842 mRNA. Translation: AAL77211.1.
IPI	IPI00206468. IPI00231939.
RefSeq	NP_062038.1. NM_019165.1.
UniGene	Rn.11118.
3D structure databases
ProteinModelPortal	P97636.
SMR	P97636. Positions 37-190.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	29197.
KEGG	rno:29197.
UCSC	RGD:2889. rat.
Organism-specific databases
CTD	3606.
RGD	2889. Il18.
Phylogenomic databases
eggNOG	NOG77229.
HOGENOM	HOG000048723.
HOVERGEN	HBG000388.
InParanoid	P97636.
KO	K05482.
OrthoDB	EOG4B8JFJ.
Gene expression databases
Genevestigator	P97636.
GermOnline	ENSRNOG00000009848. Rattus norvegicus.
Family and domain databases
InterPro	IPR008996. Cytokine_IL1-like. IPR000975. Interleukin_1. IPR015529. Interleukin_18. [Graphical view]
PANTHER	PTHR11326. PTHR11326. 1 hit.
Pfam	PF00340. IL1. 1 hit. [Graphical view]
PIRSF	PIRSF015162. Interleukin_18. 1 hit.
PRINTS	PR01933. INTRLEUKIN18.
SMART	SM00125. IL1. 1 hit. [Graphical view]
SUPFAM	SSF50353. Cytok_IL1_like. 1 hit.
ProtoNet	Search...
Other
BindingDB	P97636.
NextBio	608335.

Entry information

Entry name

IL18_RAT

Accession

Primary (citable) accession number: P97636
Secondary accession number(s): O88749, P97637, Q541E6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program