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Reviewed, UniProtKB/Swiss-Prot P97633 (KC1A_RAT)

Last modified February 9, 2010. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Casein kinase I isoform alpha
      Short name=CKI-alpha
    EC=2.7.11.1
Alternative name(s):
    CK1
Gene names
Name: Csnk1a1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. Ref.2

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Monomer. Interacts with the Axin complex By similarity.

Subcellular location

Cytoplasm By similarity. Cytoplasm › cytoskeleton › centrosome By similarity. Kinetochore By similarity. Note: Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

Contains 1 protein kinase domain.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Isoforms exhibit differences in binding and activity toward common CKI substrates.
Isoform 1 (identifier: P97633-1)

Also known as: CKI-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P97633-2)

Also known as: CKI-alpha-L;

The sequence of this isoform differs from the canonical sequence as follows:
     152-152: K → KCLESPVGKRKRSMTVSPSQDPSFSGLNQ
Note: Phosphorylated on Ser-156 (By similarity).

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 325325Casein kinase I isoform alpha
PRO_0000192826

Regions

Domain17 – 285269Protein kinase
Nucleotide binding23 – 319ATP By similarity

Sites

Active site1361Proton acceptor By similarity
Binding site461ATP By similarity

Amino acid modifications

Modified residue31Phosphoserine By similarity
Modified residue81N6-acetyllysine By similarity
Modified residue1051Phosphoserine By similarity
Modified residue3111Phosphoserine By similarity
Modified residue3131Phosphoserine By similarity
Modified residue3211Phosphothreonine By similarity

Natural variations

Alternative sequence1521K → KCLESPVGKRKRSMTVSPSQ DPSFSGLNQ in isoform 2.
VSP_004745

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (CKI-alpha) [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 484DC84B33F99BDB

FASTA32537,495
        10         20         30         40         50         60 
MASSSGSKAE FIVGGKYKLV RKIGSGSFGD IYLAINITNG EEVAVKLESQ KARHPQLLYE 

        70         80         90        100        110        120 
SKLYKILQGG VGIPHIRWYG QGKDYNVLVM DLLGPSLEDL FNFCSRRFTM KTVLMLADQM 

       130        140        150        160        170        180 
ISRIEYVHTK NFIHRDIKPD NFLMGIGRHC NKLFLIDFGL AKKYRDNRTR QHIPYREDKN 

       190        200        210        220        230        240 
LTGTARYASI NAHLGIEQSR RDDMESLGYV LMYFNRTSLP WQGLKAATKK QKYEKISEKK 

       250        260        270        280        290        300 
MSTPVEVLCK GFPAEFAMYL NYCRGLRFEE APDYMYLRQL FRILFRTLNH QYDYTFDWTM 

       310        320 
LKQKAAQQAA SSSGQGQQAQ TPTGF

« Hide

Isoform 2 (CKI-alpha-L).

Checksum: 2E25B495A5307919
Show »

FASTA35340,514

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References

[1]"Casein kinase I alpha and alpha L: alternative splicing-generated kinases exhibit different catalytic properties."
Zhang J., Gross S.D., Schroeder M.D., Anderson R.A.
Biochemistry 35:16319-16327(1996) [PubMed: 8973207] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
Strain: Sprague-Dawley.
[2]"Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism."
Liu C., Li Y., Semenov M., Han C., Baeg G.-H., Tan Y., Zhang Z., Lin X., He X.
Cell 108:837-847(2002) [PubMed: 11955436] [Abstract]
Cited for: ROLE IN WNT SIGNALING.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U77582 mRNA. Translation: AAB19227.1.
U77583 Genomic DNA. Translation: AAB19228.1.
IPIIPI00206466.
IPI00231832.
RefSeqNP_446067.1.
UniGeneRn.23810

3D structure databases

SMRP97633. Positions 10-302.
ModBaseSearch...

Protein-protein interaction databases

STRINGP97633.

PTM databases

PhosphoSiteP97633.

Genome annotation databases

EnsemblENSRNOT00000023301; ENSRNOP00000023302; ENSRNOG00000017106; Rattus norvegicus. [Genome view]
ENSRNOT00000066133; ENSRNOP00000062327; ENSRNOG00000017106; Rattus norvegicus. [Genome view]
GeneID113927.
KEGGrno:113927.

Organism-specific databases

CTD113927.
RGD71098. Csnk1a1.

Phylogenomic databases

HOVERGENP97633.
PhylomeDBP97633.

Enzyme and pathway databases

BRENDA2.7.11.1. 248.

Gene expression databases

ArrayExpressP97633.
GenevestigatorP97633.
GermOnlineENSRNOG00000017106. Rattus norvegicus.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio618038.

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Entry information

Entry nameKC1A_RAT
AccessionPrimary (citable) accession number: P97633
Secondary accession number(s): P97634
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 1, 1997
Last modified: February 9, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents