P97633 (KC1A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Casein kinase I isoform alpha
Short name=CKI-alpha
EC=2.7.11.1

Alternative name(s):
CK1

Gene names

Name:

Csnk1a1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. Ref.2
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Monomer. Interacts with the Axin complex. Interacts with TUT1, leading to TUT1 phosphorylation By similarity.
Subcellular location	Cytoplasm By similarity. Cytoplasm › cytoskeleton › centrosome By similarity. Chromosome › centromere › kinetochore By similarity. Nucleus speckle By similarity. Note: Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Wnt signaling pathway
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Kinetochore Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear speck Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from direct assay Ref.1. Source: RGD glycoprotein binding Inferred from direct assay Ref.1. Source: RGD magnesium ion binding Inferred from direct assay Ref.1. Source: RGD peptide binding Inferred from direct assay Ref.1. Source: RGD phosphoprotein binding Inferred from direct assay Ref.1. Source: RGD protein serine/threonine kinase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Isoforms exhibit differences in binding and activity toward common CKI substrates.

Isoform 1 (identifier: P97633-1)

Also known as: CKI-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P97633-2)

Also known as: CKI-alpha-L;

The sequence of this isoform differs from the canonical sequence as follows:
     152-152: K → KCLESPVGKRKRSMTVSPSQDPSFSGLNQ

Note: Contains a phosphoserine at position 156 (By similarity).

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 325	324	Casein kinase I isoform alpha		PRO_0000192826
Regions
	Domain	17 – 285	269	Protein kinase
	Nucleotide binding	23 – 31	9	ATP By similarity
Sites
	Active site	136	1	Proton acceptor By similarity
	Binding site	46	1	ATP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	8	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	152	1	K → KCLESPVGKRKRSMTVSPSQ DPSFSGLNQ in isoform 2.		VSP_004745

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CKI-alpha) [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 484DC84B33F99BDB	FASTA	325	37,495
	10 20 30 40 50 60 MASSSGSKAE FIVGGKYKLV RKIGSGSFGD IYLAINITNG EEVAVKLESQ KARHPQLLYE 70 80 90 100 110 120 SKLYKILQGG VGIPHIRWYG QGKDYNVLVM DLLGPSLEDL FNFCSRRFTM KTVLMLADQM 130 140 150 160 170 180 ISRIEYVHTK NFIHRDIKPD NFLMGIGRHC NKLFLIDFGL AKKYRDNRTR QHIPYREDKN 190 200 210 220 230 240 LTGTARYASI NAHLGIEQSR RDDMESLGYV LMYFNRTSLP WQGLKAATKK QKYEKISEKK 250 260 270 280 290 300 MSTPVEVLCK GFPAEFAMYL NYCRGLRFEE APDYMYLRQL FRILFRTLNH QYDYTFDWTM 310 320 LKQKAAQQAA SSSGQGQQAQ TPTGF
	Isoform 2 (CKI-alpha-L) [UniParc]. Checksum: 2E25B495A5307919	FASTA	353	40,514
	10 20 30 40 50 60 MASSSGSKAE FIVGGKYKLV RKIGSGSFGD IYLAINITNG EEVAVKLESQ KARHPQLLYE 70 80 90 100 110 120 SKLYKILQGG VGIPHIRWYG QGKDYNVLVM DLLGPSLEDL FNFCSRRFTM KTVLMLADQM 130 140 150 160 170 180 ISRIEYVHTK NFIHRDIKPD NFLMGIGRHC NKCLESPVGK RKRSMTVSPS QDPSFSGLNQ 190 200 210 220 230 240 LFLIDFGLAK KYRDNRTRQH IPYREDKNLT GTARYASINA HLGIEQSRRD DMESLGYVLM 250 260 270 280 290 300 YFNRTSLPWQ GLKAATKKQK YEKISEKKMS TPVEVLCKGF PAEFAMYLNY CRGLRFEEAP 310 320 330 340 350 DYMYLRQLFR ILFRTLNHQY DYTFDWTMLK QKAAQQAASS SGQGQQAQTP TGF

References

[1]	"Casein kinase I alpha and alpha L: alternative splicing-generated kinases exhibit different catalytic properties." Zhang J., Gross S.D., Schroeder M.D., Anderson R.A. Biochemistry 35:16319-16327(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2). Strain: Sprague-Dawley.
[2]	"Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism." Liu C., Li Y., Semenov M., Han C., Baeg G.-H., Tan Y., Zhang Z., Lin X., He X. Cell 108:837-847(2002) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN WNT SIGNALING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U77582 mRNA. Translation: AAB19227.1. U77583 Genomic DNA. Translation: AAB19228.1.
IPI	IPI00206466. IPI00231832.
RefSeq	NP_446067.1. NM_053615.1.
UniGene	Rn.23810.
3D structure databases
ProteinModelPortal	P97633.
SMR	P97633. Positions 10-302.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-3389838.
STRING	10116.ENSRNOP00000051516.
PTM databases
PhosphoSite	P97633.
Proteomic databases
PaxDb	P97633.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	113927.
KEGG	rno:113927.
UCSC	RGD:71098. rat.
Organism-specific databases
CTD	1452.
RGD	71098. Csnk1a1.
Phylogenomic databases
eggNOG	COG0515.
HOVERGEN	HBG000176.
KO	K08957.
Enzyme and pathway databases
BRENDA	2.7.11.1. 5301.
Gene expression databases
ArrayExpress	P97633.
Genevestigator	P97633.
GermOnline	ENSRNOG00000017106. Rattus norvegicus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P97633.
ChEMBL	CHEMBL4936.
NextBio	618038.

Entry information

Entry name

KC1A_RAT

Accession

Primary (citable) accession number: P97633
Secondary accession number(s): P97634

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program