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P97633

- KC1A_RAT

UniProt

P97633 - KC1A_RAT

Protein

Casein kinase I isoform alpha

Gene

Csnk1a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei46 – 461ATPPROSITE-ProRule annotation
    Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi23 – 319ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: RGD
    2. glycoprotein binding Source: RGD
    3. magnesium ion binding Source: RGD
    4. peptide binding Source: RGD
    5. phosphoprotein binding Source: RGD
    6. protein kinase activity Source: UniProtKB
    7. protein serine/threonine kinase activity Source: RGD

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. protein phosphorylation Source: RGD
    3. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Wnt signaling pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.1. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Casein kinase I isoform alpha (EC:2.7.11.1)
    Short name:
    CKI-alpha
    Alternative name(s):
    CK1
    Gene namesi
    Name:Csnk1a1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi71098. Csnk1a1.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Chromosomecentromerekinetochore By similarity. Nucleus speckle By similarity
    Note: Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.By similarity

    GO - Cellular componenti

    1. condensed chromosome kinetochore Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB-SubCell
    3. microtubule organizing center Source: UniProtKB-SubCell
    4. nuclear speck Source: UniProtKB

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 325324Casein kinase I isoform alphaPRO_0000192826Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei8 – 81N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP97633.
    PRIDEiP97633.

    PTM databases

    PhosphoSiteiP97633.

    Expressioni

    Gene expression databases

    GenevestigatoriP97633.

    Interactioni

    Subunit structurei

    Monomer. Interacts with the Axin complex. Interacts with TUT1, leading to TUT1 phosphorylation By similarity.By similarity

    Protein-protein interaction databases

    BioGridi250223. 2 interactions.
    IntActiP97633. 2 interactions.
    MINTiMINT-3389838.
    STRINGi10116.ENSRNOP00000051516.

    Structurei

    3D structure databases

    ProteinModelPortaliP97633.
    SMRiP97633. Positions 10-302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini17 – 285269Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOVERGENiHBG000176.
    KOiK08957.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Isoforms exhibit differences in binding and activity toward common CKI substrates.

    Isoform 1 (identifier: P97633-1) [UniParc]FASTAAdd to Basket

    Also known as: CKI-alpha

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASSSGSKAE FIVGGKYKLV RKIGSGSFGD IYLAINITNG EEVAVKLESQ    50
    KARHPQLLYE SKLYKILQGG VGIPHIRWYG QGKDYNVLVM DLLGPSLEDL 100
    FNFCSRRFTM KTVLMLADQM ISRIEYVHTK NFIHRDIKPD NFLMGIGRHC 150
    NKLFLIDFGL AKKYRDNRTR QHIPYREDKN LTGTARYASI NAHLGIEQSR 200
    RDDMESLGYV LMYFNRTSLP WQGLKAATKK QKYEKISEKK MSTPVEVLCK 250
    GFPAEFAMYL NYCRGLRFEE APDYMYLRQL FRILFRTLNH QYDYTFDWTM 300
    LKQKAAQQAA SSSGQGQQAQ TPTGF 325
    Length:325
    Mass (Da):37,495
    Last modified:May 1, 1997 - v1
    Checksum:i484DC84B33F99BDB
    GO
    Isoform 2 (identifier: P97633-2) [UniParc]FASTAAdd to Basket

    Also known as: CKI-alpha-L

    The sequence of this isoform differs from the canonical sequence as follows:
         152-152: K → KCLESPVGKRKRSMTVSPSQDPSFSGLNQ

    Note: Contains a phosphoserine at position 156.By similarity

    Show »
    Length:353
    Mass (Da):40,514
    Checksum:i2E25B495A5307919
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei152 – 1521K → KCLESPVGKRKRSMTVSPSQ DPSFSGLNQ in isoform 2. 1 PublicationVSP_004745

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77582 mRNA. Translation: AAB19227.1.
    U77583 Genomic DNA. Translation: AAB19228.1.
    RefSeqiNP_446067.1. NM_053615.1. [P97633-1]
    UniGeneiRn.23810.

    Genome annotation databases

    GeneIDi113927.
    KEGGirno:113927.
    UCSCiRGD:71098. rat. [P97633-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77582 mRNA. Translation: AAB19227.1 .
    U77583 Genomic DNA. Translation: AAB19228.1 .
    RefSeqi NP_446067.1. NM_053615.1. [P97633-1 ]
    UniGenei Rn.23810.

    3D structure databases

    ProteinModelPortali P97633.
    SMRi P97633. Positions 10-302.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250223. 2 interactions.
    IntActi P97633. 2 interactions.
    MINTi MINT-3389838.
    STRINGi 10116.ENSRNOP00000051516.

    Chemistry

    BindingDBi P97633.

    PTM databases

    PhosphoSitei P97633.

    Proteomic databases

    PaxDbi P97633.
    PRIDEi P97633.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 113927.
    KEGGi rno:113927.
    UCSCi RGD:71098. rat. [P97633-1 ]

    Organism-specific databases

    CTDi 1452.
    RGDi 71098. Csnk1a1.

    Phylogenomic databases

    eggNOGi COG0515.
    HOVERGENi HBG000176.
    KOi K08957.

    Enzyme and pathway databases

    BRENDAi 2.7.11.1. 5301.

    Miscellaneous databases

    NextBioi 618038.
    PROi P97633.

    Gene expression databases

    Genevestigatori P97633.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Casein kinase I alpha and alpha L: alternative splicing-generated kinases exhibit different catalytic properties."
      Zhang J., Gross S.D., Schroeder M.D., Anderson R.A.
      Biochemistry 35:16319-16327(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
      Strain: Sprague-Dawley.
    2. "Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism."
      Liu C., Li Y., Semenov M., Han C., Baeg G.-H., Tan Y., Zhang Z., Lin X., He X.
      Cell 108:837-847(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE IN WNT SIGNALING.

    Entry informationi

    Entry nameiKC1A_RAT
    AccessioniPrimary (citable) accession number: P97633
    Secondary accession number(s): P97634
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3