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P97633 (KC1A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Casein kinase I isoform alpha

Short name=CKI-alpha
EC=2.7.11.1
Alternative name(s):
CK1
Gene names
Name:Csnk1a1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. Ref.2

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Monomer. Interacts with the Axin complex. Interacts with TUT1, leading to TUT1 phosphorylation By similarity.

Subcellular location

Cytoplasm By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Chromosomecentromerekinetochore By similarity. Nucleus speckle By similarity. Note: Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
Wnt signaling pathway
   Cellular componentCentromere
Chromosome
Cytoplasm
Cytoskeleton
Kinetochore
Nucleus
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

mitotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

protein phosphorylation

Inferred from direct assay Ref.1. Source: RGD

   Cellular_componentcondensed chromosome kinetochore

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

microtubule organizing center

Inferred from electronic annotation. Source: UniProtKB-SubCell

nuclear speck

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from direct assay Ref.1. Source: RGD

glycoprotein binding

Inferred from direct assay Ref.1. Source: RGD

magnesium ion binding

Inferred from direct assay Ref.1. Source: RGD

peptide binding

Inferred from direct assay Ref.1. Source: RGD

phosphoprotein binding

Inferred from direct assay Ref.1. Source: RGD

protein kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein serine/threonine kinase activity

Inferred from direct assay Ref.1. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Isoforms exhibit differences in binding and activity toward common CKI substrates.
Isoform 1 (identifier: P97633-1)

Also known as: CKI-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P97633-2)

Also known as: CKI-alpha-L;

The sequence of this isoform differs from the canonical sequence as follows:
     152-152: K → KCLESPVGKRKRSMTVSPSQDPSFSGLNQ
Note: Contains a phosphoserine at position 156 (By similarity).

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 325324Casein kinase I isoform alpha
PRO_0000192826

Regions

Domain17 – 285269Protein kinase
Nucleotide binding23 – 319ATP By similarity

Sites

Active site1361Proton acceptor By similarity
Binding site461ATP By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue81N6-acetyllysine By similarity

Natural variations

Alternative sequence1521K → KCLESPVGKRKRSMTVSPSQ DPSFSGLNQ in isoform 2.
VSP_004745

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (CKI-alpha) [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 484DC84B33F99BDB

FASTA32537,495
        10         20         30         40         50         60 
MASSSGSKAE FIVGGKYKLV RKIGSGSFGD IYLAINITNG EEVAVKLESQ KARHPQLLYE 

        70         80         90        100        110        120 
SKLYKILQGG VGIPHIRWYG QGKDYNVLVM DLLGPSLEDL FNFCSRRFTM KTVLMLADQM 

       130        140        150        160        170        180 
ISRIEYVHTK NFIHRDIKPD NFLMGIGRHC NKLFLIDFGL AKKYRDNRTR QHIPYREDKN 

       190        200        210        220        230        240 
LTGTARYASI NAHLGIEQSR RDDMESLGYV LMYFNRTSLP WQGLKAATKK QKYEKISEKK 

       250        260        270        280        290        300 
MSTPVEVLCK GFPAEFAMYL NYCRGLRFEE APDYMYLRQL FRILFRTLNH QYDYTFDWTM 

       310        320 
LKQKAAQQAA SSSGQGQQAQ TPTGF 

« Hide

Isoform 2 (CKI-alpha-L) [UniParc].

Checksum: 2E25B495A5307919
Show »

FASTA35340,514

References

[1]"Casein kinase I alpha and alpha L: alternative splicing-generated kinases exhibit different catalytic properties."
Zhang J., Gross S.D., Schroeder M.D., Anderson R.A.
Biochemistry 35:16319-16327(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
Strain: Sprague-Dawley.
[2]"Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism."
Liu C., Li Y., Semenov M., Han C., Baeg G.-H., Tan Y., Zhang Z., Lin X., He X.
Cell 108:837-847(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: ROLE IN WNT SIGNALING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U77582 mRNA. Translation: AAB19227.1.
U77583 Genomic DNA. Translation: AAB19228.1.
RefSeqNP_446067.1. NM_053615.1. [P97633-1]
UniGeneRn.23810.

3D structure databases

ProteinModelPortalP97633.
SMRP97633. Positions 10-302.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid250223. 2 interactions.
IntActP97633. 2 interactions.
MINTMINT-3389838.
STRING10116.ENSRNOP00000051516.

Chemistry

BindingDBP97633.

PTM databases

PhosphoSiteP97633.

Proteomic databases

PaxDbP97633.
PRIDEP97633.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID113927.
KEGGrno:113927.
UCSCRGD:71098. rat. [P97633-1]

Organism-specific databases

CTD1452.
RGD71098. Csnk1a1.

Phylogenomic databases

eggNOGCOG0515.
HOVERGENHBG000176.
KOK08957.

Enzyme and pathway databases

BRENDA2.7.11.1. 5301.

Gene expression databases

GenevestigatorP97633.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio618038.
PROP97633.

Entry information

Entry nameKC1A_RAT
AccessionPrimary (citable) accession number: P97633
Secondary accession number(s): P97634
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families