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Protein

Casein kinase I isoform alpha

Gene

Csnk1a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei46 – 461ATPPROSITE-ProRule annotation
Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi23 – 319ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: RGD
  • glycoprotein binding Source: RGD
  • magnesium ion binding Source: RGD
  • peptide binding Source: RGD
  • phosphoprotein binding Source: RGD
  • protein kinase activity Source: UniProtKB
  • protein serine/threonine kinase activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Wnt signaling pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I isoform alpha (EC:2.7.11.1)
Short name:
CKI-alpha
Alternative name(s):
CK1
Gene namesi
Name:Csnk1a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi71098. Csnk1a1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 325324Casein kinase I isoform alphaPRO_0000192826Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei8 – 81N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP97633.
PRIDEiP97633.

PTM databases

PhosphoSiteiP97633.

Expressioni

Gene expression databases

GenevisibleiP97633. RN.

Interactioni

Subunit structurei

Monomer. Interacts with the Axin complex. Interacts with TUT1, leading to TUT1 phosphorylation (By similarity).By similarity

Protein-protein interaction databases

BioGridi250223. 2 interactions.
IntActiP97633. 2 interactions.
MINTiMINT-3389838.
STRINGi10116.ENSRNOP00000051516.

Structurei

3D structure databases

ProteinModelPortaliP97633.
SMRiP97633. Positions 10-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 285269Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOVERGENiHBG000176.
InParanoidiP97633.
KOiK08957.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Isoforms exhibit differences in binding and activity toward common CKI substrates.

Isoform 1 (identifier: P97633-1) [UniParc]FASTAAdd to basket

Also known as: CKI-alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSSGSKAE FIVGGKYKLV RKIGSGSFGD IYLAINITNG EEVAVKLESQ
60 70 80 90 100
KARHPQLLYE SKLYKILQGG VGIPHIRWYG QGKDYNVLVM DLLGPSLEDL
110 120 130 140 150
FNFCSRRFTM KTVLMLADQM ISRIEYVHTK NFIHRDIKPD NFLMGIGRHC
160 170 180 190 200
NKLFLIDFGL AKKYRDNRTR QHIPYREDKN LTGTARYASI NAHLGIEQSR
210 220 230 240 250
RDDMESLGYV LMYFNRTSLP WQGLKAATKK QKYEKISEKK MSTPVEVLCK
260 270 280 290 300
GFPAEFAMYL NYCRGLRFEE APDYMYLRQL FRILFRTLNH QYDYTFDWTM
310 320
LKQKAAQQAA SSSGQGQQAQ TPTGF
Length:325
Mass (Da):37,495
Last modified:May 1, 1997 - v1
Checksum:i484DC84B33F99BDB
GO
Isoform 2 (identifier: P97633-2) [UniParc]FASTAAdd to basket

Also known as: CKI-alpha-L

The sequence of this isoform differs from the canonical sequence as follows:
     152-152: K → KCLESPVGKRKRSMTVSPSQDPSFSGLNQ

Note: Contains a phosphoserine at position 156.By similarity
Show »
Length:353
Mass (Da):40,514
Checksum:i2E25B495A5307919
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei152 – 1521K → KCLESPVGKRKRSMTVSPSQ DPSFSGLNQ in isoform 2. 1 PublicationVSP_004745

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77582 mRNA. Translation: AAB19227.1.
U77583 Genomic DNA. Translation: AAB19228.1.
RefSeqiNP_446067.1. NM_053615.1. [P97633-1]
UniGeneiRn.23810.

Genome annotation databases

GeneIDi113927.
KEGGirno:113927.
UCSCiRGD:71098. rat. [P97633-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77582 mRNA. Translation: AAB19227.1.
U77583 Genomic DNA. Translation: AAB19228.1.
RefSeqiNP_446067.1. NM_053615.1. [P97633-1]
UniGeneiRn.23810.

3D structure databases

ProteinModelPortaliP97633.
SMRiP97633. Positions 10-302.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250223. 2 interactions.
IntActiP97633. 2 interactions.
MINTiMINT-3389838.
STRINGi10116.ENSRNOP00000051516.

Chemistry

BindingDBiP97633.

PTM databases

PhosphoSiteiP97633.

Proteomic databases

PaxDbiP97633.
PRIDEiP97633.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi113927.
KEGGirno:113927.
UCSCiRGD:71098. rat. [P97633-1]

Organism-specific databases

CTDi1452.
RGDi71098. Csnk1a1.

Phylogenomic databases

eggNOGiCOG0515.
HOVERGENiHBG000176.
InParanoidiP97633.
KOiK08957.

Enzyme and pathway databases

BRENDAi2.7.11.1. 5301.

Miscellaneous databases

NextBioi618038.
PROiP97633.

Gene expression databases

GenevisibleiP97633. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Casein kinase I alpha and alpha L: alternative splicing-generated kinases exhibit different catalytic properties."
    Zhang J., Gross S.D., Schroeder M.D., Anderson R.A.
    Biochemistry 35:16319-16327(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
    Strain: Sprague-Dawley.
  2. "Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism."
    Liu C., Li Y., Semenov M., Han C., Baeg G.-H., Tan Y., Zhang Z., Lin X., He X.
    Cell 108:837-847(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN WNT SIGNALING.

Entry informationi

Entry nameiKC1A_RAT
AccessioniPrimary (citable) accession number: P97633
Secondary accession number(s): P97634
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 1, 1997
Last modified: June 24, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.