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Protein

Leucyl-cystinyl aminopeptidase

Gene

Lnpep

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Release of an N-terminal amino acid, cleave before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain (By similarity).By similarity

Catalytic activityi

Release of an N-terminal amino acid, Cys-|-Xaa-, in which the half-cystine residue is involved in a disulfide loop, notably in oxytocin or vasopressin. Hydrolysis rates on a range of aminoacyl arylamides exceed that for the cystinyl derivative, however.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei295SubstrateBy similarity1
Metal bindingi464Zinc; catalyticPROSITE-ProRule annotation1
Active sitei465Proton acceptorPROSITE-ProRule annotation1
Metal bindingi468Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi487Zinc; catalyticPROSITE-ProRule annotation1
Sitei549Transition state stabilizerBy similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: RGD
  • angiotensin receptor activity Source: RGD
  • metalloaminopeptidase activity Source: GO_Central
  • peptide hormone binding Source: RGD
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • neuropeptide catabolic process Source: RGD
  • peptide catabolic process Source: RGD
  • positive regulation of blood pressure Source: RGD
  • regulation of long-term neuronal synaptic plasticity Source: RGD
  • response to hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucyl-cystinyl aminopeptidase (EC:3.4.11.3)
Short name:
Cystinyl aminopeptidase
Alternative name(s):
GP160
Insulin-regulated membrane aminopeptidase
Insulin-responsive aminopeptidase
Short name:
IRAP
Oxytocinase
Short name:
OTase
Placental leucine aminopeptidase
Short name:
P-LAP
Vesicle protein of 165 kDa
Short name:
Vp165
Gene namesi
Name:Lnpep
Synonyms:Irap, Otase
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621752. Lnpep.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 109CytoplasmicSequence analysisAdd BLAST109
Transmembranei110 – 131Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST22
Topological domaini132 – 1025ExtracellularSequence analysisAdd BLAST894

GO - Cellular componenti

  • cell surface Source: RGD
  • cytoplasm Source: RGD
  • insulin-responsive compartment Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: RGD
  • intracellular organelle Source: RGD
  • neuronal cell body Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • plasma membrane Source: RGD
  • vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3712.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000951151 – 1025Leucyl-cystinyl aminopeptidaseAdd BLAST1025

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei70PhosphotyrosineBy similarity1
Modified residuei80Phosphoserine; by PKC/PRKCZ; in vitro1 Publication1
Modified residuei91Phosphoserine; by PKC/PRKCZ; in vitro1 Publication1
Glycosylationi145N-linked (GlcNAc...)Sequence analysis1
Glycosylationi184N-linked (GlcNAc...)Sequence analysis1
Glycosylationi215N-linked (GlcNAc...)Sequence analysis1
Glycosylationi256N-linked (GlcNAc...)Sequence analysis1
Glycosylationi266N-linked (GlcNAc...)Sequence analysis1
Glycosylationi368N-linked (GlcNAc...)Sequence analysis1
Glycosylationi374N-linked (GlcNAc...)Sequence analysis1
Glycosylationi447N-linked (GlcNAc...)Sequence analysis1
Glycosylationi525N-linked (GlcNAc...)Sequence analysis1
Glycosylationi578N-linked (GlcNAc...)Sequence analysis1
Glycosylationi664N-linked (GlcNAc...)Sequence analysis1
Glycosylationi682N-linked (GlcNAc...)Sequence analysis1
Glycosylationi695N-linked (GlcNAc...)Sequence analysis1
Glycosylationi758N-linked (GlcNAc...)Sequence analysis1
Glycosylationi834N-linked (GlcNAc...)Sequence analysis1
Glycosylationi850N-linked (GlcNAc...)Sequence analysis1
Glycosylationi989N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP97629.
PRIDEiP97629.

PTM databases

iPTMnetiP97629.
PhosphoSitePlusiP97629.

Expressioni

Tissue specificityi

Highly expressed in heart, brain, spleen, lung, kidney and white adipose tissue. Detected at lower levels in skeletal muscle and liver.

Gene expression databases

BgeeiENSRNOG00000047387.
GenevisibleiP97629. RN.

Interactioni

Subunit structurei

Homodimer. Binds tankyrases 1 and 2 (By similarity).By similarity

Protein-protein interaction databases

IntActiP97629. 2 interactors.
STRINGi10116.ENSRNOP00000017718.

Chemistry databases

BindingDBiP97629.

Structurei

3D structure databases

ProteinModelPortaliP97629.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 101Tankyrase bindingBy similarity6
Regioni428 – 432Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi53 – 54Dileucine internalization motifSequence analysis2
Motifi76 – 77Dileucine internalization motifSequence analysis2

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1046. Eukaryota.
COG0308. LUCA.
HOGENOMiHOG000106482.
HOVERGENiHBG108296.
InParanoidiP97629.
KOiK01257.
OrthoDBiEOG091G01GH.
PhylomeDBiP97629.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97629-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METFTNDRLQ LPRNMIENSM FEEEPDVVDL AKEPCLHPLE PDEVEYEPRG
60 70 80 90 100
SRLLVRGLGE HEMDEDEEDY ESSAKLLGMS FMNRSSGLRN SATGYRQSPD
110 120 130 140 150
GTCSVPSART LVICVFVIVV AVSVIMVIYL LPRCTFTKEG CHKTNQSAEL
160 170 180 190 200
IQPIATNGKV FPWAQIRLPT AIIPQRYELS LHPNLTSMTF RGSVTISLQA
210 220 230 240 250
LQDTRDIILH STGHNISSVT FMSAVSSQEK QVEILEYPYH EQIAVVAPES
260 270 280 290 300
LLTGHNYTLK IEYSANISNS YYGFYGITYT DKSNEKKNFA ATQFEPLAAR
310 320 330 340 350
SAFPCFDEPA FKATFIIKIT RDEHHTALSN MPKKSSVPTE EGLIQDEFSE
360 370 380 390 400
SVKMSTYLVA FIVGEMRNLS QDVNGTLVSV YAVPEKIDQV YHALDTTVKL
410 420 430 440 450
LEFYQNYFEI QYPLKKLDLV AIPDFEAGAM ENWGLLTFRE ETLLYDNATS
460 470 480 490 500
SVADRKLVTK IIAHELAHQW FGNLVTMQWW NDLWLNEGFA TFMEYFSVEK
510 520 530 540 550
IFKELNSYED FLDARFKTMR KDSLNSSHPI SSSVQSSEQI EEMFDSLSYF
560 570 580 590 600
KGASLLLMLK SYLSEDVFQH AIILYLHNHS YAAIQSDDLW DSFNEVTGKT
610 620 630 640 650
LDVKKMMKTW TLQKGFPLVT VQRKGTELLL QQERFFPSMQ PEIQDSDTSH
660 670 680 690 700
LWHIPISYVT DGRNYSEYRS VSLLDKKSDV INLTEQVQWV KVNTNMTGYY
710 720 730 740 750
IVHYAHDGWA ALINQLKRNP YVLSDKDRAN LINNIFELAG LGKVPLQMAF
760 770 780 790 800
DLIDYLRNET HTAPITEALF QTDLIYNLLE KLGHMDLSSR LVTRVHKLLQ
810 820 830 840 850
NQIQQQTWTD EGTPSMRELR SALLEFACAH SLENCTTMAT KLFDGWMASN
860 870 880 890 900
GTQSLPTDVM TTVFKVGART EKGWLFLFSM YSSMGSEAEK DKILEALASS
910 920 930 940 950
ADAHKLYWLM KSSLDGDIIR TQKLSLIIRT VGRQFPGHLL AWDFVKENWN
960 970 980 990 1000
KLVHKFHLGS YTIQSIVAGS THLFSTKTHL SEVQEFFENQ SEATLQLRCV
1010 1020
QEAFEVIELN IQWMARNLKT LTLWL
Length:1,025
Mass (Da):117,201
Last modified:May 1, 1997 - v1
Checksum:i8AD3BA3A446FB5EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti909 – 916LMKSSLDG → YGTTQRAW in AAB38021 (PubMed:7559527).Curated8
Sequence conflicti916 – 1025Missing in AAB38021 (PubMed:7559527).CuratedAdd BLAST110

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76997 mRNA. Translation: AAB19066.1.
U32990 mRNA. Translation: AAB38021.1.
PIRiI55441.
RefSeqiNP_001106874.1. NM_001113403.1.
NP_598258.1. NM_133574.1.
XP_008757040.1. XM_008758818.2.
XP_017443357.1. XM_017587868.1.
UniGeneiRn.10614.

Genome annotation databases

GeneIDi108348118.
171105.
KEGGirno:100912445.
rno:171105.
UCSCiRGD:621752. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76997 mRNA. Translation: AAB19066.1.
U32990 mRNA. Translation: AAB38021.1.
PIRiI55441.
RefSeqiNP_001106874.1. NM_001113403.1.
NP_598258.1. NM_133574.1.
XP_008757040.1. XM_008758818.2.
XP_017443357.1. XM_017587868.1.
UniGeneiRn.10614.

3D structure databases

ProteinModelPortaliP97629.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP97629. 2 interactors.
STRINGi10116.ENSRNOP00000017718.

Chemistry databases

BindingDBiP97629.
ChEMBLiCHEMBL3712.

Protein family/group databases

MEROPSiM01.011.

PTM databases

iPTMnetiP97629.
PhosphoSitePlusiP97629.

Proteomic databases

PaxDbiP97629.
PRIDEiP97629.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi108348118.
171105.
KEGGirno:100912445.
rno:171105.
UCSCiRGD:621752. rat.

Organism-specific databases

CTDi4012.
RGDi621752. Lnpep.

Phylogenomic databases

eggNOGiKOG1046. Eukaryota.
COG0308. LUCA.
HOGENOMiHOG000106482.
HOVERGENiHBG108296.
InParanoidiP97629.
KOiK01257.
OrthoDBiEOG091G01GH.
PhylomeDBiP97629.

Miscellaneous databases

PROiP97629.

Gene expression databases

BgeeiENSRNOG00000047387.
GenevisibleiP97629. RN.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLCAP_RAT
AccessioniPrimary (citable) accession number: P97629
Secondary accession number(s): Q11009
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.