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Protein

Securin

Gene

Pttg1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of p53/TP53. The negative regulation of p53/TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Chromosome partition, DNA damage, DNA repair, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-174154 APC/C:Cdc20 mediated degradation of Securin
R-RNO-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-RNO-2467813 Separation of Sister Chromatids

Names & Taxonomyi

Protein namesi
Recommended name:
Securin
Alternative name(s):
Pituitary tumor-transforming gene 1 protein
Gene namesi
Name:Pttg1
Synonyms:Pttg
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi68359 Pttg1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Has strong transforming capabilities on a variety of cell lines including NIH 3T3 fibroblasts and on athymic nude mice. Overexpressed in animals suffering from pituitary adenomas. The transforming capability may be due to its interaction and regulation of p53/TP53 pathway.1 Publication

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002063642 – 199SecurinAdd BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei162PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-162 by CDK1 during mitosis.By similarity
Phosphorylated in vitro by ds-DNA kinase.By similarity
Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP97613
PRIDEiP97613

PTM databases

iPTMnetiP97613
PhosphoSitePlusiP97613

Expressioni

Tissue specificityi

Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Expressed in both spermatocytes and spermatids.2 Publications

Gene expression databases

BgeeiENSRNOG00000003802
ExpressionAtlasiP97613 baseline and differential
GenevisibleiP97613 RN

Interactioni

Subunit structurei

Interacts with the caspase-like ESPL1, and prevents its protease activity by covering its active site. Interacts with p53/TP53 and blocks its activity probably by blocking its binding to DNA. Interacts with the Ku 70 kDa subunit of ds-DNA kinase. Interacts with PTTG1IP (By similarity). Interacts with RPS10 and DNAJA1.By similarity1 Publication

GO - Molecular functioni

  • heat shock protein binding Source: RGD
  • SH3 domain binding Source: UniProtKB-KW

Protein-protein interaction databases

BioGridi249000, 3 interactors
STRINGi10116.ENSRNOP00000005070

Structurei

3D structure databases

ProteinModelPortaliP97613
SMRiP97613
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 61D-box4
Motifi68 – 70TEK-box 13
Motifi91 – 93TEK-box 23
Motifi179 – 192SH3-bindingAdd BLAST14

Domaini

The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.By similarity
The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thioester with the acceptor lysine residue (By similarity).By similarity

Sequence similaritiesi

Belongs to the securin family.Curated

Keywords - Domaini

Repeat, SH3-binding

Phylogenomic databases

eggNOGiENOG410IVQH Eukaryota
ENOG410Z8MR LUCA
GeneTreeiENSGT00390000009693
HOGENOMiHOG000035097
HOVERGENiHBG053264
InParanoidiP97613
KOiK06635
OMAiFSAKKVT
OrthoDBiEOG091G0PAH
PhylomeDBiP97613
TreeFamiTF330797

Family and domain databases

InterProiView protein in InterPro
IPR006940 Securin_separation_inhibitor
PANTHERiPTHR10418 PTHR10418, 1 hit
PfamiView protein in Pfam
PF04856 Securin, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97613-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLIFVDKD NEEPGSRLAS KDGLKLGSGV KALDGKLQVS TPRVGKVFGA
60 70 80 90 100
PGLPKASRKA LGTVNRVTEK PVKSSKPLQS KQPTLSVKKI TEKSTKTQGS
110 120 130 140 150
APAPDDAYPE IEKFFPFDPL DFESFDLPEE HQISLLPLNG VPLMILNEER
160 170 180 190
GLEKLLHLDP PSPLQKPFLP WESDPLPSPP SALSALDVEL PPVCYDADI
Length:199
Mass (Da):21,571
Last modified:May 1, 1997 - v1
Checksum:i07D89438000FE378
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73030 mRNA Translation: AAB47974.1
AF021802 Genomic DNA Translation: AAC40036.1
RefSeqiNP_071786.1, NM_022391.3
XP_006246204.1, XM_006246142.1
XP_006246205.1, XM_006246143.3
XP_006246206.1, XM_006246144.1
XP_006246207.1, XM_006246145.3
UniGeneiRn.137183
Rn.271

Genome annotation databases

EnsembliENSRNOT00000005070; ENSRNOP00000005070; ENSRNOG00000003802
GeneIDi64193
KEGGirno:64193
UCSCiRGD:68359 rat

Similar proteinsi

Entry informationi

Entry nameiPTTG1_RAT
AccessioniPrimary (citable) accession number: P97613
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: May 1, 1997
Last modified: March 28, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health