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P97613 (PTTG1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Securin
Alternative name(s):
Pituitary tumor-transforming gene 1 protein
Gene names
Name:Pttg1
Synonyms:Pttg
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length199 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of p53/TP53. The negative regulation of p53/TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation By similarity.

Subunit structure

Interacts with the caspase-like ESPL1, and prevents its protease activity by covering its active site. Interacts with p53/TP53 and blocks its activity probably by blocking its binding to DNA. Interacts with the Ku 70 kDa subunit of ds-DNA kinase. Interacts with PTTG1IP By similarity. Interacts with RPS10 and DNAJA1. Ref.3

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Tissue specificity

Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Expressed in both spermatocytes and spermatids. Ref.1 Ref.3

Domain

The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway By similarity.

The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thioester with the acceptor lysine residue By similarity.

Post-translational modification

Phosphorylated at Ser-162 by CDK1 during mitosis By similarity.

Phosphorylated in vitro by ds-DNA kinase By similarity.

Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10 By similarity.

Involvement in disease

Has strong transforming capabilities on a variety of cell lines including NIH 3T3 fibroblasts and on athymic nude mice. Overexpressed in animals suffering from pituitary adenomas. The transforming capability may be due to its interaction and regulation of p53/TP53 pathway. Ref.1

Sequence similarities

Belongs to the securin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 199198Securin
PRO_0000206364

Regions

Motif58 – 614D-box
Motif68 – 703TEK-box 1
Motif91 – 933TEK-box 2
Motif179 – 19214SH3-binding

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue1621Phosphoserine; by CDK1 By similarity

Sequences

Sequence LengthMass (Da)Tools
P97613 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 07D89438000FE378

FASTA19921,571
        10         20         30         40         50         60 
MATLIFVDKD NEEPGSRLAS KDGLKLGSGV KALDGKLQVS TPRVGKVFGA PGLPKASRKA 

        70         80         90        100        110        120 
LGTVNRVTEK PVKSSKPLQS KQPTLSVKKI TEKSTKTQGS APAPDDAYPE IEKFFPFDPL 

       130        140        150        160        170        180 
DFESFDLPEE HQISLLPLNG VPLMILNEER GLEKLLHLDP PSPLQKPFLP WESDPLPSPP 

       190 
SALSALDVEL PPVCYDADI 

« Hide

References

[1]"Isolation and characterization of a pituitary tumor-transforming gene (PTTG)."
Pei L., Melmed S.
Mol. Endocrinol. 11:433-441(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DISEASE.
Tissue: Pituitary tumor.
[2]"Genomic organization and identification of an enhancer element containing binding sites for multiple proteins in rat pituitary tumor-transforming gene."
Pei L.
J. Biol. Chem. 273:5219-5225(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
Strain: Sprague-Dawley.
[3]"Pituitary tumor-transforming gene protein associates with ribosomal protein S10 and a novel human homologue of DnaJ in testicular cells."
Pei L.
J. Biol. Chem. 274:3151-3158(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INTERACTION WITH RPS10 AND DNAJA1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U73030 mRNA. Translation: AAB47974.1.
AF021802 Genomic DNA. Translation: AAC40036.1.
RefSeqNP_071786.1. NM_022391.2.
XP_006246204.1. XM_006246142.1.
XP_006246205.1. XM_006246143.1.
XP_006246206.1. XM_006246144.1.
XP_006246207.1. XM_006246145.1.
UniGeneRn.137183.
Rn.271.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP97613.
PRIDEP97613.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000005070; ENSRNOP00000005070; ENSRNOG00000003802.
GeneID64193.
KEGGrno:64193.
UCSCRGD:68359. rat.

Organism-specific databases

CTD9232.
RGD68359. Pttg1.

Phylogenomic databases

eggNOGNOG42896.
GeneTreeENSGT00390000009693.
HOGENOMHOG000035097.
HOVERGENHBG053264.
InParanoidP97613.
KOK06635.
OMAGTRVAPK.
OrthoDBEOG7MPRH0.
PhylomeDBP97613.
TreeFamTF330797.

Gene expression databases

ArrayExpressP97613.
GenevestigatorP97613.

Family and domain databases

InterProIPR018008. Securin_separation-inh_met.
IPR006940. Securin_separation_inhibitor.
[Graphical view]
PANTHERPTHR10418. PTHR10418. 1 hit.
PfamPF04856. Securin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio612860.
PROP97613.

Entry information

Entry namePTTG1_RAT
AccessionPrimary (citable) accession number: P97613
Entry history
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families