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P97613

- PTTG1_RAT

UniProt

P97613 - PTTG1_RAT

Protein

Securin

Gene

Pttg1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of p53/TP53. The negative regulation of p53/TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation By similarity.By similarity

    GO - Molecular functioni

    1. cysteine-type endopeptidase inhibitor activity Source: Ensembl
    2. heat shock protein binding Source: RGD
    3. ribosome binding Source: RGD

    GO - Biological processi

    1. chromosome organization Source: InterPro
    2. DNA repair Source: UniProtKB-KW
    3. homologous chromosome segregation Source: Ensembl
    4. mitotic nuclear division Source: UniProtKB-KW
    5. negative regulation of cell proliferation Source: RGD
    6. regulation of cell growth Source: RGD

    Keywords - Biological processi

    Cell cycle, Cell division, Chromosome partition, DNA damage, DNA repair, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_194781. Separation of Sister Chromatids.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Securin
    Alternative name(s):
    Pituitary tumor-transforming gene 1 protein
    Gene namesi
    Name:Pttg1
    Synonyms:Pttg
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi68359. Pttg1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    Has strong transforming capabilities on a variety of cell lines including NIH 3T3 fibroblasts and on athymic nude mice. Overexpressed in animals suffering from pituitary adenomas. The transforming capability may be due to its interaction and regulation of p53/TP53 pathway.1 Publication

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 199198SecurinPRO_0000206364Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei162 – 1621Phosphoserine; by CDK1By similarity

    Post-translational modificationi

    Phosphorylated at Ser-162 by CDK1 during mitosis.By similarity
    Phosphorylated in vitro by ds-DNA kinase.By similarity
    Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10 By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP97613.
    PRIDEiP97613.

    Expressioni

    Tissue specificityi

    Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Expressed in both spermatocytes and spermatids.2 Publications

    Gene expression databases

    ArrayExpressiP97613.
    GenevestigatoriP97613.

    Interactioni

    Subunit structurei

    Interacts with the caspase-like ESPL1, and prevents its protease activity by covering its active site. Interacts with p53/TP53 and blocks its activity probably by blocking its binding to DNA. Interacts with the Ku 70 kDa subunit of ds-DNA kinase. Interacts with PTTG1IP By similarity. Interacts with RPS10 and DNAJA1.By similarity1 Publication

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 614D-box
    Motifi68 – 703TEK-box 1
    Motifi91 – 933TEK-box 2
    Motifi179 – 19214SH3-bindingAdd
    BLAST

    Domaini

    The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.By similarity
    The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thioester with the acceptor lysine residue By similarity.By similarity

    Sequence similaritiesi

    Belongs to the securin family.Curated

    Keywords - Domaini

    Repeat, SH3-binding

    Phylogenomic databases

    eggNOGiNOG42896.
    GeneTreeiENSGT00390000009693.
    HOGENOMiHOG000035097.
    HOVERGENiHBG053264.
    InParanoidiP97613.
    KOiK06635.
    OMAiGTRVAPK.
    OrthoDBiEOG7MPRH0.
    PhylomeDBiP97613.
    TreeFamiTF330797.

    Family and domain databases

    InterProiIPR018008. Securin_separation-inh_met.
    IPR006940. Securin_separation_inhibitor.
    [Graphical view]
    PANTHERiPTHR10418. PTHR10418. 1 hit.
    PfamiPF04856. Securin. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97613-1 [UniParc]FASTAAdd to Basket

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    MATLIFVDKD NEEPGSRLAS KDGLKLGSGV KALDGKLQVS TPRVGKVFGA    50
    PGLPKASRKA LGTVNRVTEK PVKSSKPLQS KQPTLSVKKI TEKSTKTQGS 100
    APAPDDAYPE IEKFFPFDPL DFESFDLPEE HQISLLPLNG VPLMILNEER 150
    GLEKLLHLDP PSPLQKPFLP WESDPLPSPP SALSALDVEL PPVCYDADI 199
    Length:199
    Mass (Da):21,571
    Last modified:May 1, 1997 - v1
    Checksum:i07D89438000FE378
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73030 mRNA. Translation: AAB47974.1.
    AF021802 Genomic DNA. Translation: AAC40036.1.
    RefSeqiNP_071786.1. NM_022391.2.
    XP_006246204.1. XM_006246142.1.
    XP_006246205.1. XM_006246143.1.
    XP_006246206.1. XM_006246144.1.
    XP_006246207.1. XM_006246145.1.
    UniGeneiRn.137183.
    Rn.271.

    Genome annotation databases

    EnsembliENSRNOT00000005070; ENSRNOP00000005070; ENSRNOG00000003802.
    GeneIDi64193.
    KEGGirno:64193.
    UCSCiRGD:68359. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73030 mRNA. Translation: AAB47974.1 .
    AF021802 Genomic DNA. Translation: AAC40036.1 .
    RefSeqi NP_071786.1. NM_022391.2.
    XP_006246204.1. XM_006246142.1.
    XP_006246205.1. XM_006246143.1.
    XP_006246206.1. XM_006246144.1.
    XP_006246207.1. XM_006246145.1.
    UniGenei Rn.137183.
    Rn.271.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P97613.
    PRIDEi P97613.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000005070 ; ENSRNOP00000005070 ; ENSRNOG00000003802 .
    GeneIDi 64193.
    KEGGi rno:64193.
    UCSCi RGD:68359. rat.

    Organism-specific databases

    CTDi 9232.
    RGDi 68359. Pttg1.

    Phylogenomic databases

    eggNOGi NOG42896.
    GeneTreei ENSGT00390000009693.
    HOGENOMi HOG000035097.
    HOVERGENi HBG053264.
    InParanoidi P97613.
    KOi K06635.
    OMAi GTRVAPK.
    OrthoDBi EOG7MPRH0.
    PhylomeDBi P97613.
    TreeFami TF330797.

    Enzyme and pathway databases

    Reactomei REACT_194781. Separation of Sister Chromatids.

    Miscellaneous databases

    NextBioi 612860.
    PROi P97613.

    Gene expression databases

    ArrayExpressi P97613.
    Genevestigatori P97613.

    Family and domain databases

    InterProi IPR018008. Securin_separation-inh_met.
    IPR006940. Securin_separation_inhibitor.
    [Graphical view ]
    PANTHERi PTHR10418. PTHR10418. 1 hit.
    Pfami PF04856. Securin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a pituitary tumor-transforming gene (PTTG)."
      Pei L., Melmed S.
      Mol. Endocrinol. 11:433-441(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DISEASE.
      Tissue: Pituitary tumor.
    2. "Genomic organization and identification of an enhancer element containing binding sites for multiple proteins in rat pituitary tumor-transforming gene."
      Pei L.
      J. Biol. Chem. 273:5219-5225(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
      Strain: Sprague-Dawley.
    3. "Pituitary tumor-transforming gene protein associates with ribosomal protein S10 and a novel human homologue of DnaJ in testicular cells."
      Pei L.
      J. Biol. Chem. 274:3151-3158(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INTERACTION WITH RPS10 AND DNAJA1.

    Entry informationi

    Entry nameiPTTG1_RAT
    AccessioniPrimary (citable) accession number: P97613
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 25, 2003
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3