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P97613

- PTTG1_RAT

UniProt

P97613 - PTTG1_RAT

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Protein

Securin

Gene

Pttg1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of p53/TP53. The negative regulation of p53/TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation By similarity.By similarity

GO - Molecular functioni

  1. cysteine-type endopeptidase inhibitor activity Source: Ensembl
  2. heat shock protein binding Source: RGD
  3. ribosome binding Source: RGD

GO - Biological processi

  1. chromosome organization Source: InterPro
  2. DNA repair Source: UniProtKB-KW
  3. homologous chromosome segregation Source: Ensembl
  4. mitotic nuclear division Source: UniProtKB-KW
  5. negative regulation of cell proliferation Source: RGD
  6. regulation of cell growth Source: RGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, DNA damage, DNA repair, Mitosis

Enzyme and pathway databases

ReactomeiREACT_194781. Separation of Sister Chromatids.

Names & Taxonomyi

Protein namesi
Recommended name:
Securin
Alternative name(s):
Pituitary tumor-transforming gene 1 protein
Gene namesi
Name:Pttg1
Synonyms:Pttg
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi68359. Pttg1.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Has strong transforming capabilities on a variety of cell lines including NIH 3T3 fibroblasts and on athymic nude mice. Overexpressed in animals suffering from pituitary adenomas. The transforming capability may be due to its interaction and regulation of p53/TP53 pathway.1 Publication

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 199198SecurinPRO_0000206364Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei162 – 1621Phosphoserine; by CDK1By similarity

Post-translational modificationi

Phosphorylated at Ser-162 by CDK1 during mitosis.By similarity
Phosphorylated in vitro by ds-DNA kinase.By similarity
Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10 By similarity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP97613.
PRIDEiP97613.

Expressioni

Tissue specificityi

Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Expressed in both spermatocytes and spermatids.2 Publications

Gene expression databases

ExpressionAtlasiP97613. baseline and differential.
GenevestigatoriP97613.

Interactioni

Subunit structurei

Interacts with the caspase-like ESPL1, and prevents its protease activity by covering its active site. Interacts with p53/TP53 and blocks its activity probably by blocking its binding to DNA. Interacts with the Ku 70 kDa subunit of ds-DNA kinase. Interacts with PTTG1IP By similarity. Interacts with RPS10 and DNAJA1.By similarity1 Publication

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 614D-box
Motifi68 – 703TEK-box 1
Motifi91 – 933TEK-box 2
Motifi179 – 19214SH3-bindingAdd
BLAST

Domaini

The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.By similarity
The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thioester with the acceptor lysine residue By similarity.By similarity

Sequence similaritiesi

Belongs to the securin family.Curated

Keywords - Domaini

Repeat, SH3-binding

Phylogenomic databases

eggNOGiNOG42896.
GeneTreeiENSGT00390000009693.
HOGENOMiHOG000035097.
HOVERGENiHBG053264.
InParanoidiP97613.
KOiK06635.
OMAiGTRVAPK.
OrthoDBiEOG7MPRH0.
PhylomeDBiP97613.
TreeFamiTF330797.

Family and domain databases

InterProiIPR018008. Securin_separation-inh_met.
IPR006940. Securin_separation_inhibitor.
[Graphical view]
PANTHERiPTHR10418. PTHR10418. 1 hit.
PfamiPF04856. Securin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97613-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATLIFVDKD NEEPGSRLAS KDGLKLGSGV KALDGKLQVS TPRVGKVFGA
60 70 80 90 100
PGLPKASRKA LGTVNRVTEK PVKSSKPLQS KQPTLSVKKI TEKSTKTQGS
110 120 130 140 150
APAPDDAYPE IEKFFPFDPL DFESFDLPEE HQISLLPLNG VPLMILNEER
160 170 180 190
GLEKLLHLDP PSPLQKPFLP WESDPLPSPP SALSALDVEL PPVCYDADI
Length:199
Mass (Da):21,571
Last modified:May 1, 1997 - v1
Checksum:i07D89438000FE378
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U73030 mRNA. Translation: AAB47974.1.
AF021802 Genomic DNA. Translation: AAC40036.1.
RefSeqiNP_071786.1. NM_022391.2.
XP_006246204.1. XM_006246142.1.
XP_006246205.1. XM_006246143.2.
XP_006246206.1. XM_006246144.1.
XP_006246207.1. XM_006246145.2.
UniGeneiRn.137183.
Rn.271.

Genome annotation databases

EnsembliENSRNOT00000005070; ENSRNOP00000005070; ENSRNOG00000003802.
GeneIDi64193.
KEGGirno:64193.
UCSCiRGD:68359. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U73030 mRNA. Translation: AAB47974.1 .
AF021802 Genomic DNA. Translation: AAC40036.1 .
RefSeqi NP_071786.1. NM_022391.2.
XP_006246204.1. XM_006246142.1.
XP_006246205.1. XM_006246143.2.
XP_006246206.1. XM_006246144.1.
XP_006246207.1. XM_006246145.2.
UniGenei Rn.137183.
Rn.271.

3D structure databases

ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P97613.
PRIDEi P97613.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000005070 ; ENSRNOP00000005070 ; ENSRNOG00000003802 .
GeneIDi 64193.
KEGGi rno:64193.
UCSCi RGD:68359. rat.

Organism-specific databases

CTDi 9232.
RGDi 68359. Pttg1.

Phylogenomic databases

eggNOGi NOG42896.
GeneTreei ENSGT00390000009693.
HOGENOMi HOG000035097.
HOVERGENi HBG053264.
InParanoidi P97613.
KOi K06635.
OMAi GTRVAPK.
OrthoDBi EOG7MPRH0.
PhylomeDBi P97613.
TreeFami TF330797.

Enzyme and pathway databases

Reactomei REACT_194781. Separation of Sister Chromatids.

Miscellaneous databases

NextBioi 612860.
PROi P97613.

Gene expression databases

ExpressionAtlasi P97613. baseline and differential.
Genevestigatori P97613.

Family and domain databases

InterProi IPR018008. Securin_separation-inh_met.
IPR006940. Securin_separation_inhibitor.
[Graphical view ]
PANTHERi PTHR10418. PTHR10418. 1 hit.
Pfami PF04856. Securin. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and characterization of a pituitary tumor-transforming gene (PTTG)."
    Pei L., Melmed S.
    Mol. Endocrinol. 11:433-441(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DISEASE.
    Tissue: Pituitary tumor.
  2. "Genomic organization and identification of an enhancer element containing binding sites for multiple proteins in rat pituitary tumor-transforming gene."
    Pei L.
    J. Biol. Chem. 273:5219-5225(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
    Strain: Sprague-Dawley.
  3. "Pituitary tumor-transforming gene protein associates with ribosomal protein S10 and a novel human homologue of DnaJ in testicular cells."
    Pei L.
    J. Biol. Chem. 274:3151-3158(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH RPS10 AND DNAJA1.

Entry informationi

Entry nameiPTTG1_RAT
AccessioniPrimary (citable) accession number: P97613
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3