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Protein

5-oxoprolinase

Gene

Oplah

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.

Catalytic activityi

ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10109.
BRENDAi3.5.2.9. 5301.
ReactomeiREACT_314885. Glutathione synthesis and recycling.

Names & Taxonomyi

Protein namesi
Recommended name:
5-oxoprolinase (EC:3.5.2.9)
Alternative name(s):
5-oxo-L-prolinase
Short name:
5-OPase
Pyroglutamase
Gene namesi
Name:Oplah
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi620956. Oplah.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 128812885-oxoprolinasePRO_0000208579Add
BLAST

Proteomic databases

PaxDbiP97608.
PRIDEiP97608.

Expressioni

Tissue specificityi

Well expressed in testis, kidney and liver.

Gene expression databases

GenevisibleiP97608. RN.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016424.

Structurei

3D structure databases

ProteinModelPortaliP97608.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the oxoprolinase family.Curated

Phylogenomic databases

eggNOGiCOG0146.
GeneTreeiENSGT00390000013463.
HOGENOMiHOG000047200.
HOVERGENiHBG007870.
InParanoidiP97608.
KOiK01469.
OMAiGNARMGY.
OrthoDBiEOG78M013.
PhylomeDBiP97608.
TreeFamiTF300520.

Family and domain databases

InterProiIPR008040. Hydant_A_N.
IPR002821. Hydantoinase_A.
IPR003692. Hydantoinase_B.
[Graphical view]
PfamiPF05378. Hydant_A_N. 1 hit.
PF01968. Hydantoinase_A. 1 hit.
PF02538. Hydantoinase_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97608-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSPEGRFHF AIDRGGTFTD VFAQCPGGHV RVLKLLSEDP ANYADAPTEG
60 70 80 90 100
IRRILEQEEG VLLPRGRPLD TSRIASIRMG TTVATNALLE RQGERVALLV
110 120 130 140 150
TRGFRDLLHI GTQARPDLFD LAVPMPEVLY EEVLEVDERV VLYRGEPGAG
160 170 180 190 200
SPVKGRTGDL LEIQQPVDLE ALRGKLEGLL SRGIHSLAVV LMHSYTWAQH
210 220 230 240 250
EQQVGTLARE LGFTHVSLSS EVMPMVRIVP RGHTACADAY LTPTIQRYVQ
260 270 280 290 300
GFRRGFQGQL KNVQVLFMRS DGGLAPMDAF SGSRAVLSGP AGGVVGYSAT
310 320 330 340 350
TYHLEGGQPV IGFDMGGTST DVSRYAGEFE HVFEASTAGV TLQAPQLDIN
360 370 380 390 400
TVAAGGGSRL FFRSGLFVVG PESAGAHPGP ACYRKGGPVT VTDANLVLGR
410 420 430 440 450
LLPASFPCIF GPGEDQPLSP EASRKALEAV AMEVNSFLTN GPCPASQLSL
460 470 480 490 500
EEVAMGFVRV ANEAMCRPIR ALTQARGHDP SAHVLACFGG AGGQHACAIA
510 520 530 540 550
RALGMDTVHI HRHSGLLSAL GLALADVVHE AQEPCSLSYT PETFAQLDQR
560 570 580 590 600
LSRLEEQCVD ALQVQGFPRS QISTESFLHL RYQGTDCALM VSAHQHPATA
610 620 630 640 650
CSPRAGDFGA AFVERYMREF GFIIPERPVV VDDVRVRGTG RSGLQLEDTP
660 670 680 690 700
KIQTGPPHVE KVTQCYFEGG YQETPVYLLG ELGYGHQLQG PCLIIDNNST
710 720 730 740 750
ILVEPGCQAE VTDTGDIRIS VGAEGPSMAD TRLDPIQLSI FSHRFMSIAE
760 770 780 790 800
QMGRILQRTA ISTNIKERLD FSCALFGPDG GLVSNAPHIP VHLGAMQETV
810 820 830 840 850
QFQIQHLGAD LHPGDVLLSN HPSAGGSHLP DLTVITPVFW PGQTRPVFYV
860 870 880 890 900
ASRGHHADIG GITPGSMPPH STTLQQEGAV FLSFKLVQGG VFQEEAVTEA
910 920 930 940 950
LRAPGKISGC SGTRNLHDNL SDLRAQVAAN QKGIQLVGEL IGQYGLDVVQ
960 970 980 990 1000
AYMGHIQANA ELAVRDMLRA FGTSRQARGL PLEVSAEDHM DDGSPICLRV
1010 1020 1030 1040 1050
QINLSQGSAV FDFTGSGSEV FGNLNAPRAI TLSALIYCLR CLVGRDIPLN
1060 1070 1080 1090 1100
QGCLAPVRVI IPKGSILDPS PEAAVVGGNV LTSQRVVDVI LGAFGACSAS
1110 1120 1130 1140 1150
QGCMNNVTLG NARMGYYETV AGGAGAGPGW HGRSGVHSHM TNTRITDPEI
1160 1170 1180 1190 1200
LESRYPVILR RFELRPGSGG RGRFRGGDGV VRELVFREEA LLSVLTERRA
1210 1220 1230 1240 1250
FQPYGLHGGE PGARGLNLLI RKDGRTVNLG GKTSVTVYPG DVFCLHTPGG
1260 1270 1280
GGYGDPEDPA PPPGSPPLFP AFPERGSVFE YRRAQEAV
Length:1,288
Mass (Da):137,720
Last modified:February 15, 2005 - v2
Checksum:i23DA4809D60D5730
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441A → P in AAC52955 (PubMed:8943290).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70825 mRNA. Translation: AAC52955.1.
BC085330 mRNA. Translation: AAH85330.1.
PIRiT42756.
RefSeqiNP_446356.1. NM_053904.1.
XP_006241836.1. XM_006241774.2.
XP_006241837.1. XM_006241775.2.
UniGeneiRn.3066.

Genome annotation databases

EnsembliENSRNOT00000016424; ENSRNOP00000016424; ENSRNOG00000011781.
GeneIDi116684.
KEGGirno:116684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70825 mRNA. Translation: AAC52955.1.
BC085330 mRNA. Translation: AAH85330.1.
PIRiT42756.
RefSeqiNP_446356.1. NM_053904.1.
XP_006241836.1. XM_006241774.2.
XP_006241837.1. XM_006241775.2.
UniGeneiRn.3066.

3D structure databases

ProteinModelPortaliP97608.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016424.

Proteomic databases

PaxDbiP97608.
PRIDEiP97608.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016424; ENSRNOP00000016424; ENSRNOG00000011781.
GeneIDi116684.
KEGGirno:116684.

Organism-specific databases

CTDi26873.
RGDi620956. Oplah.

Phylogenomic databases

eggNOGiCOG0146.
GeneTreeiENSGT00390000013463.
HOGENOMiHOG000047200.
HOVERGENiHBG007870.
InParanoidiP97608.
KOiK01469.
OMAiGNARMGY.
OrthoDBiEOG78M013.
PhylomeDBiP97608.
TreeFamiTF300520.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10109.
BRENDAi3.5.2.9. 5301.
ReactomeiREACT_314885. Glutathione synthesis and recycling.

Miscellaneous databases

NextBioi619536.
PROiP97608.

Gene expression databases

GenevisibleiP97608. RN.

Family and domain databases

InterProiIPR008040. Hydant_A_N.
IPR002821. Hydantoinase_A.
IPR003692. Hydantoinase_B.
[Graphical view]
PfamiPF05378. Hydant_A_N. 1 hit.
PF01968. Hydantoinase_A. 1 hit.
PF02538. Hydantoinase_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The amino acid sequence of rat kidney 5-oxo-L-prolinase determined by cDNA cloning."
    Ye G.-J., Breslow E., Meister A.
    J. Biol. Chem. 271:32293-32300(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. Erratum
    Ye G.-J., Breslow E., Meister A.
    J. Biol. Chem. 272:4646-4646(1997)
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiOPLA_RAT
AccessioniPrimary (citable) accession number: P97608
Secondary accession number(s): Q5U3Z8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 15, 2005
Last modified: June 24, 2015
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.