Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P97608 (OPLA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-oxoprolinase

EC=3.5.2.9
Alternative name(s):
5-oxo-L-prolinase
Short name=5-OPase
Pyroglutamase
Gene names
Name:Oplah
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1288 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.

Catalytic activity

ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.

Subunit structure

Homodimer.

Tissue specificity

Well expressed in testis, kidney and liver.

Sequence similarities

Belongs to the oxoprolinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 128812885-oxoprolinase
PRO_0000208579

Experimental info

Sequence conflict441A → P in AAC52955. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P97608 [UniParc].

Last modified February 15, 2005. Version 2.
Checksum: 23DA4809D60D5730

FASTA1,288137,720
        10         20         30         40         50         60 
MGSPEGRFHF AIDRGGTFTD VFAQCPGGHV RVLKLLSEDP ANYADAPTEG IRRILEQEEG 

        70         80         90        100        110        120 
VLLPRGRPLD TSRIASIRMG TTVATNALLE RQGERVALLV TRGFRDLLHI GTQARPDLFD 

       130        140        150        160        170        180 
LAVPMPEVLY EEVLEVDERV VLYRGEPGAG SPVKGRTGDL LEIQQPVDLE ALRGKLEGLL 

       190        200        210        220        230        240 
SRGIHSLAVV LMHSYTWAQH EQQVGTLARE LGFTHVSLSS EVMPMVRIVP RGHTACADAY 

       250        260        270        280        290        300 
LTPTIQRYVQ GFRRGFQGQL KNVQVLFMRS DGGLAPMDAF SGSRAVLSGP AGGVVGYSAT 

       310        320        330        340        350        360 
TYHLEGGQPV IGFDMGGTST DVSRYAGEFE HVFEASTAGV TLQAPQLDIN TVAAGGGSRL 

       370        380        390        400        410        420 
FFRSGLFVVG PESAGAHPGP ACYRKGGPVT VTDANLVLGR LLPASFPCIF GPGEDQPLSP 

       430        440        450        460        470        480 
EASRKALEAV AMEVNSFLTN GPCPASQLSL EEVAMGFVRV ANEAMCRPIR ALTQARGHDP 

       490        500        510        520        530        540 
SAHVLACFGG AGGQHACAIA RALGMDTVHI HRHSGLLSAL GLALADVVHE AQEPCSLSYT 

       550        560        570        580        590        600 
PETFAQLDQR LSRLEEQCVD ALQVQGFPRS QISTESFLHL RYQGTDCALM VSAHQHPATA 

       610        620        630        640        650        660 
CSPRAGDFGA AFVERYMREF GFIIPERPVV VDDVRVRGTG RSGLQLEDTP KIQTGPPHVE 

       670        680        690        700        710        720 
KVTQCYFEGG YQETPVYLLG ELGYGHQLQG PCLIIDNNST ILVEPGCQAE VTDTGDIRIS 

       730        740        750        760        770        780 
VGAEGPSMAD TRLDPIQLSI FSHRFMSIAE QMGRILQRTA ISTNIKERLD FSCALFGPDG 

       790        800        810        820        830        840 
GLVSNAPHIP VHLGAMQETV QFQIQHLGAD LHPGDVLLSN HPSAGGSHLP DLTVITPVFW 

       850        860        870        880        890        900 
PGQTRPVFYV ASRGHHADIG GITPGSMPPH STTLQQEGAV FLSFKLVQGG VFQEEAVTEA 

       910        920        930        940        950        960 
LRAPGKISGC SGTRNLHDNL SDLRAQVAAN QKGIQLVGEL IGQYGLDVVQ AYMGHIQANA 

       970        980        990       1000       1010       1020 
ELAVRDMLRA FGTSRQARGL PLEVSAEDHM DDGSPICLRV QINLSQGSAV FDFTGSGSEV 

      1030       1040       1050       1060       1070       1080 
FGNLNAPRAI TLSALIYCLR CLVGRDIPLN QGCLAPVRVI IPKGSILDPS PEAAVVGGNV 

      1090       1100       1110       1120       1130       1140 
LTSQRVVDVI LGAFGACSAS QGCMNNVTLG NARMGYYETV AGGAGAGPGW HGRSGVHSHM 

      1150       1160       1170       1180       1190       1200 
TNTRITDPEI LESRYPVILR RFELRPGSGG RGRFRGGDGV VRELVFREEA LLSVLTERRA 

      1210       1220       1230       1240       1250       1260 
FQPYGLHGGE PGARGLNLLI RKDGRTVNLG GKTSVTVYPG DVFCLHTPGG GGYGDPEDPA 

      1270       1280 
PPPGSPPLFP AFPERGSVFE YRRAQEAV 

« Hide

References

« Hide 'large scale' references
[1]"The amino acid sequence of rat kidney 5-oxo-L-prolinase determined by cDNA cloning."
Ye G.-J., Breslow E., Meister A.
J. Biol. Chem. 271:32293-32300(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: Sprague-Dawley.
Tissue: Kidney.
[2]Erratum
Ye G.-J., Breslow E., Meister A.
J. Biol. Chem. 272:4646-4646(1997)
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U70825 mRNA. Translation: AAC52955.1.
BC085330 mRNA. Translation: AAH85330.1.
PIRT42756.
RefSeqNP_446356.1. NM_053904.1.
XP_006241836.1. XM_006241774.1.
XP_006241837.1. XM_006241775.1.
UniGeneRn.3066.

3D structure databases

ProteinModelPortalP97608.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000016424.

Proteomic databases

PaxDbP97608.
PRIDEP97608.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000016424; ENSRNOP00000016424; ENSRNOG00000011781.
GeneID116684.
KEGGrno:116684.

Organism-specific databases

CTD26873.
RGD620956. Oplah.

Phylogenomic databases

eggNOGCOG0146.
GeneTreeENSGT00390000013463.
HOGENOMHOG000047200.
HOVERGENHBG007870.
InParanoidP97608.
KOK01469.
OMACQAEVTD.
OrthoDBEOG78M013.
PhylomeDBP97608.
TreeFamTF300520.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-10109.

Gene expression databases

GenevestigatorP97608.

Family and domain databases

InterProIPR008040. Hydant_A_N.
IPR002821. Hydantoinase_A.
IPR003692. Hydantoinase_B.
[Graphical view]
PfamPF05378. Hydant_A_N. 1 hit.
PF01968. Hydantoinase_A. 1 hit.
PF02538. Hydantoinase_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio619536.
PROP97608.

Entry information

Entry nameOPLA_RAT
AccessionPrimary (citable) accession number: P97608
Secondary accession number(s): Q5U3Z8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 15, 2005
Last modified: April 16, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families