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P97608

- OPLA_RAT

UniProt

P97608 - OPLA_RAT

Protein

5-oxoprolinase

Gene

Oplah

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.

    Catalytic activityi

    ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.

    GO - Molecular functioni

    1. 5-oxoprolinase (ATP-hydrolyzing) activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-10109.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-oxoprolinase (EC:3.5.2.9)
    Alternative name(s):
    5-oxo-L-prolinase
    Short name:
    5-OPase
    Pyroglutamase
    Gene namesi
    Name:Oplah
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi620956. Oplah.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 128812885-oxoprolinasePRO_0000208579Add
    BLAST

    Proteomic databases

    PaxDbiP97608.
    PRIDEiP97608.

    Expressioni

    Tissue specificityi

    Well expressed in testis, kidney and liver.

    Gene expression databases

    GenevestigatoriP97608.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000016424.

    Structurei

    3D structure databases

    ProteinModelPortaliP97608.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the oxoprolinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0146.
    GeneTreeiENSGT00390000013463.
    HOGENOMiHOG000047200.
    HOVERGENiHBG007870.
    InParanoidiP97608.
    KOiK01469.
    OMAiTERMINI.
    OrthoDBiEOG78M013.
    PhylomeDBiP97608.
    TreeFamiTF300520.

    Family and domain databases

    InterProiIPR008040. Hydant_A_N.
    IPR002821. Hydantoinase_A.
    IPR003692. Hydantoinase_B.
    [Graphical view]
    PfamiPF05378. Hydant_A_N. 1 hit.
    PF01968. Hydantoinase_A. 1 hit.
    PF02538. Hydantoinase_B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P97608-1 [UniParc]FASTAAdd to Basket

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    MGSPEGRFHF AIDRGGTFTD VFAQCPGGHV RVLKLLSEDP ANYADAPTEG     50
    IRRILEQEEG VLLPRGRPLD TSRIASIRMG TTVATNALLE RQGERVALLV 100
    TRGFRDLLHI GTQARPDLFD LAVPMPEVLY EEVLEVDERV VLYRGEPGAG 150
    SPVKGRTGDL LEIQQPVDLE ALRGKLEGLL SRGIHSLAVV LMHSYTWAQH 200
    EQQVGTLARE LGFTHVSLSS EVMPMVRIVP RGHTACADAY LTPTIQRYVQ 250
    GFRRGFQGQL KNVQVLFMRS DGGLAPMDAF SGSRAVLSGP AGGVVGYSAT 300
    TYHLEGGQPV IGFDMGGTST DVSRYAGEFE HVFEASTAGV TLQAPQLDIN 350
    TVAAGGGSRL FFRSGLFVVG PESAGAHPGP ACYRKGGPVT VTDANLVLGR 400
    LLPASFPCIF GPGEDQPLSP EASRKALEAV AMEVNSFLTN GPCPASQLSL 450
    EEVAMGFVRV ANEAMCRPIR ALTQARGHDP SAHVLACFGG AGGQHACAIA 500
    RALGMDTVHI HRHSGLLSAL GLALADVVHE AQEPCSLSYT PETFAQLDQR 550
    LSRLEEQCVD ALQVQGFPRS QISTESFLHL RYQGTDCALM VSAHQHPATA 600
    CSPRAGDFGA AFVERYMREF GFIIPERPVV VDDVRVRGTG RSGLQLEDTP 650
    KIQTGPPHVE KVTQCYFEGG YQETPVYLLG ELGYGHQLQG PCLIIDNNST 700
    ILVEPGCQAE VTDTGDIRIS VGAEGPSMAD TRLDPIQLSI FSHRFMSIAE 750
    QMGRILQRTA ISTNIKERLD FSCALFGPDG GLVSNAPHIP VHLGAMQETV 800
    QFQIQHLGAD LHPGDVLLSN HPSAGGSHLP DLTVITPVFW PGQTRPVFYV 850
    ASRGHHADIG GITPGSMPPH STTLQQEGAV FLSFKLVQGG VFQEEAVTEA 900
    LRAPGKISGC SGTRNLHDNL SDLRAQVAAN QKGIQLVGEL IGQYGLDVVQ 950
    AYMGHIQANA ELAVRDMLRA FGTSRQARGL PLEVSAEDHM DDGSPICLRV 1000
    QINLSQGSAV FDFTGSGSEV FGNLNAPRAI TLSALIYCLR CLVGRDIPLN 1050
    QGCLAPVRVI IPKGSILDPS PEAAVVGGNV LTSQRVVDVI LGAFGACSAS 1100
    QGCMNNVTLG NARMGYYETV AGGAGAGPGW HGRSGVHSHM TNTRITDPEI 1150
    LESRYPVILR RFELRPGSGG RGRFRGGDGV VRELVFREEA LLSVLTERRA 1200
    FQPYGLHGGE PGARGLNLLI RKDGRTVNLG GKTSVTVYPG DVFCLHTPGG 1250
    GGYGDPEDPA PPPGSPPLFP AFPERGSVFE YRRAQEAV 1288
    Length:1,288
    Mass (Da):137,720
    Last modified:February 15, 2005 - v2
    Checksum:i23DA4809D60D5730
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti44 – 441A → P in AAC52955. (PubMed:8943290)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U70825 mRNA. Translation: AAC52955.1.
    BC085330 mRNA. Translation: AAH85330.1.
    PIRiT42756.
    RefSeqiNP_446356.1. NM_053904.1.
    XP_006241836.1. XM_006241774.1.
    XP_006241837.1. XM_006241775.1.
    UniGeneiRn.3066.

    Genome annotation databases

    EnsembliENSRNOT00000016424; ENSRNOP00000016424; ENSRNOG00000011781.
    GeneIDi116684.
    KEGGirno:116684.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U70825 mRNA. Translation: AAC52955.1 .
    BC085330 mRNA. Translation: AAH85330.1 .
    PIRi T42756.
    RefSeqi NP_446356.1. NM_053904.1.
    XP_006241836.1. XM_006241774.1.
    XP_006241837.1. XM_006241775.1.
    UniGenei Rn.3066.

    3D structure databases

    ProteinModelPortali P97608.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000016424.

    Proteomic databases

    PaxDbi P97608.
    PRIDEi P97608.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000016424 ; ENSRNOP00000016424 ; ENSRNOG00000011781 .
    GeneIDi 116684.
    KEGGi rno:116684.

    Organism-specific databases

    CTDi 26873.
    RGDi 620956. Oplah.

    Phylogenomic databases

    eggNOGi COG0146.
    GeneTreei ENSGT00390000013463.
    HOGENOMi HOG000047200.
    HOVERGENi HBG007870.
    InParanoidi P97608.
    KOi K01469.
    OMAi TERMINI.
    OrthoDBi EOG78M013.
    PhylomeDBi P97608.
    TreeFami TF300520.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-10109.

    Miscellaneous databases

    NextBioi 619536.
    PROi P97608.

    Gene expression databases

    Genevestigatori P97608.

    Family and domain databases

    InterProi IPR008040. Hydant_A_N.
    IPR002821. Hydantoinase_A.
    IPR003692. Hydantoinase_B.
    [Graphical view ]
    Pfami PF05378. Hydant_A_N. 1 hit.
    PF01968. Hydantoinase_A. 1 hit.
    PF02538. Hydantoinase_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The amino acid sequence of rat kidney 5-oxo-L-prolinase determined by cDNA cloning."
      Ye G.-J., Breslow E., Meister A.
      J. Biol. Chem. 271:32293-32300(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    2. Erratum
      Ye G.-J., Breslow E., Meister A.
      J. Biol. Chem. 272:4646-4646(1997)
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.

    Entry informationi

    Entry nameiOPLA_RAT
    AccessioniPrimary (citable) accession number: P97608
    Secondary accession number(s): Q5U3Z8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3