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P97605

- EFNA5_RAT

UniProt

P97605 - EFNA5_RAT

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Protein

Ephrin-A5

Gene

Efna5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion (By similarity).By similarity

GO - Molecular functioni

  1. ephrin receptor binding Source: UniProtKB

GO - Biological processi

  1. axonal fasciculation Source: RGD
  2. axon guidance Source: RefGenome
  3. brain development Source: RGD
  4. central nervous system development Source: RGD
  5. ephrin receptor signaling pathway Source: UniProtKB
  6. positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
  7. regulation of actin cytoskeleton organization Source: UniProtKB
  8. regulation of cell-cell adhesion Source: UniProtKB
  9. regulation of focal adhesion assembly Source: UniProtKB
  10. regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  11. regulation of microtubule cytoskeleton organization Source: UniProtKB
  12. regulation of Rac GTPase activity Source: UniProtKB
  13. regulation of Rho GTPase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin-A5
Alternative name(s):
AL-1
EPH-related receptor tyrosine kinase ligand 7
Short name:
LERK-7
Gene namesi
Name:Efna5
Synonyms:Eplg7, Lerk7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620391. Efna5.

Subcellular locationi

Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity. Membranecaveola By similarity; Lipid-anchorGPI-anchor By similarity
Note: Compartmentalized in discrete caveolae-like membrane microdomains.By similarity

GO - Cellular componenti

  1. anchored component of external side of plasma membrane Source: UniProtKB
  2. anchored component of plasma membrane Source: RefGenome
  3. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 203183Ephrin-A5PRO_0000008381Add
BLAST
Propeptidei204 – 22825Removed in mature formSequence AnalysisPRO_0000008382Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi62 ↔ 102PROSITE-ProRule annotation
Disulfide bondi90 ↔ 151PROSITE-ProRule annotation
Lipidationi203 – 2031GPI-anchor amidated asparagineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PRIDEiP97605.

Expressioni

Tissue specificityi

Expressed in brain, heart, placenta and lung.

Gene expression databases

GenevestigatoriP97605.

Interactioni

Subunit structurei

Binds to the receptor tyrosine kinases EPHA2, EPHA3, EPHB1 and EPHB2. Interacts with EPHA8; activates EPHA8. Forms a ternary EFNA5-EPHA3-ADAM10 complex mediating EFNA5 extracellular domain shedding by ADAM10 which regulates the EFNA5-EPHA3 complex internalization and function (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039406.

Structurei

3D structure databases

ProteinModelPortaliP97605.
SMRiP97605. Positions 28-165.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 162134Ephrin RBDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ephrin family.PROSITE-ProRule annotation
Contains 1 ephrin RBD (ephrin receptor-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG241965.
HOGENOMiHOG000234373.
HOVERGENiHBG051447.
InParanoidiP97605.
KOiK05462.
PhylomeDBiP97605.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view]
PANTHERiPTHR11304. PTHR11304. 1 hit.
PfamiPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSiPR01347. EPHRIN.
ProDomiPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97605-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLHVEMLTLL FLVLWMCVFS QDPGSKVVAD RYAVYWNSSN PRFQRGDYHI
60 70 80 90 100
DVCINDYLDV FCPHYEDSVP EDKTERYVLY MVNFDGYSAC DHTSKGFKRW
110 120 130 140 150
ECNRPHSPNG PLKFSEKFQL FTPFSLGFEF RPGREYFYIS SAIPDNGRRS
160 170 180 190 200
CLKLKVFVRP TNSCMKTIGV RDRVFDVNDK VENSLEPADD TVHESAEPSR
210 220
GENAAQTPRI PSRLLAILLF LLAMLLTL
Length:228
Mass (Da):26,358
Last modified:December 15, 1998 - v2
Checksum:i855985532D580022
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69279 mRNA. Translation: AAC05801.1.
RefSeqiNP_446355.1. NM_053903.1.
UniGeneiRn.10714.

Genome annotation databases

GeneIDi116683.
KEGGirno:116683.
UCSCiRGD:620391. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69279 mRNA. Translation: AAC05801.1 .
RefSeqi NP_446355.1. NM_053903.1.
UniGenei Rn.10714.

3D structure databases

ProteinModelPortali P97605.
SMRi P97605. Positions 28-165.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000039406.

Proteomic databases

PRIDEi P97605.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 116683.
KEGGi rno:116683.
UCSCi RGD:620391. rat.

Organism-specific databases

CTDi 1946.
RGDi 620391. Efna5.

Phylogenomic databases

eggNOGi NOG241965.
HOGENOMi HOG000234373.
HOVERGENi HBG051447.
InParanoidi P97605.
KOi K05462.
PhylomeDBi P97605.

Miscellaneous databases

NextBioi 619532.
PROi P97605.

Gene expression databases

Genevestigatori P97605.

Family and domain databases

Gene3Di 2.60.40.420. 1 hit.
InterProi IPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view ]
PANTHERi PTHR11304. PTHR11304. 1 hit.
Pfami PF00812. Ephrin. 1 hit.
[Graphical view ]
PRINTSi PR01347. EPHRIN.
ProDomi PD002533. Ephrin. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF49503. SSF49503. 1 hit.
PROSITEi PS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of AL-1, a ligand for an Eph-related tyrosine kinase receptor involved in axon bundle formation."
    Winslow J.W., Moran P., Valverde J., Shih A., Yuan J.Q., Wong S.C., Tsai S.P., Goddard A., Henzel W.J., Hefti F., Beck K.D., Caras I.W.
    Neuron 14:973-981(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
  2. "rLERK7, rat ligand for Eph-related receptor tyrosine kinase."
    Li Y.Y., McTiernan C.F., Feldman A.M.
    Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiEFNA5_RAT
AccessioniPrimary (citable) accession number: P97605
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: October 29, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3