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P97594 (MCPT8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mast cell protease 8

Short name=rMCP-8
EC=3.4.21.-
Alternative name(s):
Mast cell protease VIII
Short name=rMCP-VIII
Gene names
Name:Mcpt8
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Subcellular location

Secreted. Cytoplasmic granule. Note: Secretory granules.

Tissue specificity

Mast cells.

Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Propeptide19 – 202Activation peptide
PRO_0000027447
Chain21 – 248228Mast cell protease 8
PRO_0000027448

Regions

Domain21 – 243223Peptidase S1

Sites

Active site651Charge relay system By similarity
Active site1081Charge relay system By similarity
Active site2011Charge relay system By similarity

Amino acid modifications

Glycosylation671N-linked (GlcNAc...) Potential
Glycosylation1521N-linked (GlcNAc...) Potential
Glycosylation1801N-linked (GlcNAc...) Potential
Disulfide bond50 ↔ 66 By similarity
Disulfide bond142 ↔ 207 By similarity
Disulfide bond172 ↔ 186 By similarity

Sequences

Sequence LengthMass (Da)Tools
P97594 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 913178B7F033DD8E

FASTA24827,486
        10         20         30         40         50         60 
MFLFLFFLVA ILPVNTEGGE IIWGTESKPH SRPYMASLMF YYGNSYRHYC GGFLVAKDIV 

        70         80         90        100        110        120 
MTAAHCNGSN IKVTLGAHNI KKQEKTQVIA VVKAKPHENY DRHSRFNDIM LLKLERKAQL 

       130        140        150        160        170        180 
NGAVKTIALP RSQDWVKPGQ VCTVAGWGCL ANCSLSNTLQ EVNLEVQEGQ KCEDMSRNYN 

       190        200        210        220        230        240 
DSIQLCVGNP SEGKATGKGD SGGPFVCDGV AQGIVSYRLC TGTLPRVFTR ISSFIPWIQK 


TMKLLQQS 

« Hide

References

[1]"Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations."
Lutzelschwab C., Pejler G., Aveskogh M., Hellman L.
J. Exp. Med. 185:13-29(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U67911 mRNA. Translation: AAB48264.1.
RefSeqNP_067609.1. NM_021598.2.
XP_003751517.1. XM_003751469.2.
UniGeneRn.55163.

3D structure databases

ProteinModelPortalP97594.
SMRP97594. Positions 21-243.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000027950.

Protein family/group databases

MEROPSS01.009.

Proteomic databases

PaxDbP97594.
PRIDEP97594.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000027950; ENSRNOP00000027950; ENSRNOG00000047190.
ENSRNOT00000072426; ENSRNOP00000067099; ENSRNOG00000049991.
GeneID29269.
691603.
KEGGrno:29269.
rno:691603.
UCSCRGD:3067. rat.

Organism-specific databases

CTD17231.
RGD3067. Mcpt8.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00740000115234.
HOGENOMHOG000251820.
HOVERGENHBG013304.
KOK01362.
OrthoDBEOG7RRF7Z.
PhylomeDBP97594.
TreeFamTF333630.

Gene expression databases

GenevestigatorP97594.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio608612.

Entry information

Entry nameMCPT8_RAT
AccessionPrimary (citable) accession number: P97594
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries