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Protein

Mast cell protease 8

Gene

Mcpt8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay systemBy similarity
Active sitei108 – 1081Charge relay systemBy similarity
Active sitei201 – 2011Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: GO_Central

GO - Biological processi

  1. proteolysis Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Mast cell protease 8 (EC:3.4.21.-)
Short name:
rMCP-8
Alternative name(s):
Mast cell protease VIII
Short name:
rMCP-VIII
Gene namesi
Name:Mcpt8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 15

Organism-specific databases

RGDi3067. Mcpt8.

Subcellular locationi

Secreted. Cytoplasmic granule
Note: Secretory granules.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. secretory granule Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Propeptidei19 – 202Activation peptidePRO_0000027447
Chaini21 – 248228Mast cell protease 8PRO_0000027448Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Glycosylationi67 – 671N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi142 ↔ 207PROSITE-ProRule annotation
Glycosylationi152 – 1521N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi172 ↔ 186PROSITE-ProRule annotation
Glycosylationi180 – 1801N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP97594.
PRIDEiP97594.

Expressioni

Tissue specificityi

Mast cells.

Gene expression databases

GenevestigatoriP97594.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027950.

Structurei

3D structure databases

ProteinModelPortaliP97594.
SMRiP97594. Positions 21-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 243223Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP97594.
KOiK01362.
OMAiICAKENK.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP97594.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97594-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFLFLFFLVA ILPVNTEGGE IIWGTESKPH SRPYMASLMF YYGNSYRHYC
60 70 80 90 100
GGFLVAKDIV MTAAHCNGSN IKVTLGAHNI KKQEKTQVIA VVKAKPHENY
110 120 130 140 150
DRHSRFNDIM LLKLERKAQL NGAVKTIALP RSQDWVKPGQ VCTVAGWGCL
160 170 180 190 200
ANCSLSNTLQ EVNLEVQEGQ KCEDMSRNYN DSIQLCVGNP SEGKATGKGD
210 220 230 240
SGGPFVCDGV AQGIVSYRLC TGTLPRVFTR ISSFIPWIQK TMKLLQQS
Length:248
Mass (Da):27,486
Last modified:May 1, 1997 - v1
Checksum:i913178B7F033DD8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67911 mRNA. Translation: AAB48264.1.
RefSeqiNP_067609.1. NM_021598.2.
XP_003751517.1. XM_003751469.3.
UniGeneiRn.55163.

Genome annotation databases

EnsembliENSRNOT00000027950; ENSRNOP00000027950; ENSRNOG00000047190.
ENSRNOT00000072426; ENSRNOP00000067099; ENSRNOG00000049991.
GeneIDi29269.
691603.
KEGGirno:29269.
rno:691603.
UCSCiRGD:3067. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67911 mRNA. Translation: AAB48264.1.
RefSeqiNP_067609.1. NM_021598.2.
XP_003751517.1. XM_003751469.3.
UniGeneiRn.55163.

3D structure databases

ProteinModelPortaliP97594.
SMRiP97594. Positions 21-243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027950.

Protein family/group databases

MEROPSiS01.009.

Proteomic databases

PaxDbiP97594.
PRIDEiP97594.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027950; ENSRNOP00000027950; ENSRNOG00000047190.
ENSRNOT00000072426; ENSRNOP00000067099; ENSRNOG00000049991.
GeneIDi29269.
691603.
KEGGirno:29269.
rno:691603.
UCSCiRGD:3067. rat.

Organism-specific databases

CTDi17231.
RGDi3067. Mcpt8.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP97594.
KOiK01362.
OMAiICAKENK.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP97594.
TreeFamiTF333630.

Miscellaneous databases

NextBioi608612.

Gene expression databases

GenevestigatoriP97594.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations."
    Lutzelschwab C., Pejler G., Aveskogh M., Hellman L.
    J. Exp. Med. 185:13-29(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.

Entry informationi

Entry nameiMCPT8_RAT
AccessioniPrimary (citable) accession number: P97594
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: February 4, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.