P97592 (MCPT4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mast cell protease 4
Short name=rMCP-4
EC=3.4.21.-

Alternative name(s):
Mast cell protease IV
Short name=rMCP-IV

Gene names

Name:

Mcpt4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Secreted. Cytoplasmic granule. Note: Secretory granules.
Tissue specificity	Mast cells.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from direct assay PubMed 11896050. Source: RGD
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heparin binding Inferred from direct assay PubMed 11896050. Source: RGD peptide binding Inferred from direct assay PubMed 11896050. Source: RGD serine-type endopeptidase activity Inferred from direct assay PubMed 11896050. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 20	2	Activation peptide		PRO_0000027445
	Chain	21 – 246	226	Mast cell protease 4		PRO_0000027446
Regions
	Domain	21 – 244	224	Peptidase S1
Sites
	Active site	65	1	Charge relay system By similarity
	Active site	109	1	Charge relay system By similarity
	Active site	202	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	143 ↔ 208		By similarity
	Disulfide bond	174 ↔ 187		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97592 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 412FF51D05098778	FASTA	246	27,042
	10 20 30 40 50 60 MKALLFLMAL LLPSGAGAEE IIGGVESIPH SRPYMALLKI VTEEGHVTFC GGFLISLQFV 70 80 90 100 110 120 LTAAHCHGRE ITVTLGAHDM SKRESTQQKI KVVKQIFPLK YNLFSNFRDI MLLKLEQKAV 130 140 150 160 170 180 LTPSVNVIPL PQSSDIIKPG TMCLAAGWGQ TGVKEPNSNT LREVMLRIME MKACKDYRHY 190 200 210 220 230 240 DNRFQICVGI PQMLKLAYKG DSGGPLVCAG VAHGIVSHGP GRGIPPIIFT RISSYVSWIN RVIRGN

References

[1]	"Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations." Lutzelschwab C., Pejler G., Aveskogh M., Hellman L. J. Exp. Med. 185:13-29(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U67907 mRNA. Translation: AAB48260.1.
IPI	IPI00205671.
RefSeq	NP_062194.1. NM_019321.2.
UniGene	Rn.10698.
3D structure databases
ProteinModelPortal	P97592.
SMR	P97592. Positions 21-243.
ModBase	Search...
Protein family/group databases
MEROPS	S01.005.
Proteomic databases
PaxDb	P97592.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000027918; ENSRNOP00000027918; ENSRNOG00000024785.
GeneID	54270.
KEGG	rno:54270.
UCSC	RGD:3064. rat.
Organism-specific databases
CTD	17227.
RGD	3064. Mcpt4.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00700000104027.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P97592.
KO	K01362.
OMA	WINRVIR.
OrthoDB	EOG4R23VK.
Gene expression databases
Genevestigator	P97592.
GermOnline	ENSRNOG00000024785. Rattus norvegicus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	610828.

Entry information

Entry name

MCPT4_RAT

Accession

Primary (citable) accession number: P97592

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program