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Protein

Cell growth regulator with EF hand domain protein 1

Gene

Cgref1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates cell-cell adhesion in a calcium-dependent manner. Able to inhibit growth in several cell lines.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi84 – 95121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi128 – 139122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • cell cycle arrest Source: UniProtKB-KW
  • negative regulation of cell growth Source: RGD
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Cell cycle, Growth arrest

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cell growth regulator with EF hand domain protein 1
Alternative name(s):
Cell growth regulatory gene 11 protein
Hydrophobestin
Gene namesi
Name:Cgref1
Synonyms:Cgr11
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620801. Cgref1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 281260Cell growth regulator with EF hand domain protein 1PRO_0000073876Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei217 – 2171PhosphoserineCombined sources
Modified residuei228 – 2281PhosphoserineCombined sources

Post-translational modificationi

Probably digested extracellularly by an unknown serine protease generating extremely hydrophobic bioactive peptides.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP97586.
PRIDEiP97586.

Expressioni

Tissue specificityi

Expressed predominantly in whole brain and kidney, with limited expression in heart, lung, liver, and skeletal muscle and no expression in spleen and testis. Also expressed in pituitary gland, adrenal gland, digestive tract, and reproductive organs.1 Publication

Inductioni

By p53.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010586.

Structurei

3D structure databases

ProteinModelPortaliP97586.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini71 – 10636EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini115 – 15036EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Domaini

Both EF-hands are required for function.

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410J4PJ. Eukaryota.
ENOG41128UK. LUCA.
HOGENOMiHOG000074093.
HOVERGENiHBG050931.
InParanoidiP97586.
OrthoDBiEOG725DHK.
PhylomeDBiP97586.
TreeFamiTF315801.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97586-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRWLMQMLM LPLLLLPLGQ AAPKDGVARL DPEAQQQLTT NPFQPGPEQL
60 70 80 90 100
RRLRDYLKGL EKMEEDPEQM NREQVLLYLF ALHDFDQNGQ LDGLELLSML
110 120 130 140 150
TAALAPGAAH FPINPVILVV DMVLETQDLD GDGLMTPAEL INFPGEAPKR
160 170 180 190 200
AESLPPALQE PQPAGSQPLL ANSPLQSETQ QSLGTKEEIT SQVEAKRALE
210 220 230 240 250
PEQEAGHHIE TKVDALSPEG EARGQAESEG DAPGPREDAE RQVESKDNEG
260 270 280
EAKDLPAETL ETQNTPNVVE AHSIQLENDE I
Length:281
Mass (Da):30,835
Last modified:May 1, 1997 - v1
Checksum:iC8195BED34BB1549
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66470 mRNA. Translation: AAC52950.1.
RefSeqiNP_620787.2. NM_139087.2.
UniGeneiRn.31842.

Genome annotation databases

GeneIDi245918.
KEGGirno:245918.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66470 mRNA. Translation: AAC52950.1.
RefSeqiNP_620787.2. NM_139087.2.
UniGeneiRn.31842.

3D structure databases

ProteinModelPortaliP97586.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010586.

Proteomic databases

PaxDbiP97586.
PRIDEiP97586.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi245918.
KEGGirno:245918.

Organism-specific databases

CTDi10669.
RGDi620801. Cgref1.

Phylogenomic databases

eggNOGiENOG410J4PJ. Eukaryota.
ENOG41128UK. LUCA.
HOGENOMiHOG000074093.
HOVERGENiHBG050931.
InParanoidiP97586.
OrthoDBiEOG725DHK.
PhylomeDBiP97586.
TreeFamiTF315801.

Miscellaneous databases

NextBioi623158.
PROiP97586.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Induction of cell growth regulatory genes by p53."
    Madden S.L., Galella E.A., Riley D., Bertelsen A.H., Beaudry G.A.
    Cancer Res. 56:5384-5390(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Fischer.
    Tissue: Fibroblast.
  2. Cited for: FUNCTION, CALCIUM-BINDING, SUBCELLULAR LOCATION, PTM, TISSUE SPECIFICITY.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217 AND SER-228, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCGRE1_RAT
AccessioniPrimary (citable) accession number: P97586
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: May 1, 1997
Last modified: May 11, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.