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Protein

Prostaglandin reductase 1

Gene

Ptgr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity towards PGE1, PGE2 and PGE2-alpha. Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4 (By similarity).By similarity

Catalytic activityi

A n-alkanal + NAD(P)+ = an alk-2-enal + NAD(P)H.
11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei178 – 1781NADPBy similarity
Binding sitei193 – 1931NADPBy similarity
Binding sitei217 – 2171NADPBy similarity
Binding sitei321 – 3211NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi152 – 1554NADPBy similarity
Nucleotide bindingi239 – 2457NADPBy similarity
Nucleotide bindingi270 – 2723NADPBy similarity

GO - Molecular functioni

GO - Biological processi

  • response to antineoplastic agent Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin reductase 1 (EC:1.3.1.-)
Short name:
PRG-1
Alternative name(s):
15-oxoprostaglandin 13-reductase (EC:1.3.1.48)
Dithiolethione-inducible gene 1 protein
Short name:
D3T-inducible gene 1 protein
Short name:
DIG-1
NADP-dependent leukotriene B4 12-hydroxydehydrogenase (EC:1.3.1.74)
Gene namesi
Name:Ptgr1
Synonyms:Dig1, Ltb4dh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi621195. Ptgr1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4166.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Prostaglandin reductase 1PRO_0000218069Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181PhosphothreonineBy similarity
Modified residuei20 – 201PhosphoserineBy similarity
Modified residuei178 – 1781N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP97584.
PRIDEiP97584.

PTM databases

iPTMnetiP97584.

Expressioni

Inductioni

Up-regulated by 1,2-dithiole-3-thione (D3T).1 Publication

Gene expression databases

GenevisibleiP97584. RN.

Interactioni

Subunit structurei

Monomer or homodimer.By similarity

Protein-protein interaction databases

MINTiMINT-4567058.
STRINGi10116.ENSRNOP00000020335.

Chemistry

BindingDBiP97584.

Structurei

3D structure databases

ProteinModelPortaliP97584.
SMRiP97584. Positions 1-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi250 – 2578Pro-rich

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1196. Eukaryota.
COG2130. LUCA.
GeneTreeiENSGT00390000009335.
HOGENOMiHOG000294663.
HOVERGENiHBG055024.
InParanoidiP97584.
KOiK13948.
OMAiYNRTGPP.
OrthoDBiEOG7JT6WX.
PhylomeDBiP97584.
TreeFamiTF324201.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR014190. B4_12hDH.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02825. B4_12hDH. 1 hit.

Sequencei

Sequence statusi: Complete.

P97584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQAKTWTLK KHFEGFPTDS NFELRTTELP PLNNGEVLLE ALFLSVDPYM
60 70 80 90 100
RVAAKKLKEG DSMMGEQVAR VVESKNSAFP TGTIVVALLG WTSHSISDGN
110 120 130 140 150
GLRKLPAEWP DKLPLSLALG TVGMPGLTAY FGLLDICGLK GGETVLVNAA
160 170 180 190 200
AGAVGSVVGQ IAKLKGCKVV GTAGSDEKVA YLKKLGFDVA FNYKTVKSLE
210 220 230 240 250
EALRTASPDG YDCYFDNVGG EFSNTVILQM KTFGRIAICG AISQYNRTGP
260 270 280 290 300
CPPGPSPEVI IYQQLRMEGF IVTRWQGEVR QKALTDLMNW VSEGKIRYHE
310 320
YITEGFEKMP AAFMGMLKGD NLGKTIVKA
Length:329
Mass (Da):35,718
Last modified:May 1, 2000 - v3
Checksum:i8E91165086A05BA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66322 mRNA. Translation: AAB88912.2.
BC089775 mRNA. Translation: AAH89775.1.
RefSeqiNP_620218.1. NM_138863.2.
UniGeneiRn.10656.

Genome annotation databases

EnsembliENSRNOT00000020335; ENSRNOP00000020335; ENSRNOG00000015072.
GeneIDi192227.
KEGGirno:192227.
UCSCiRGD:621195. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66322 mRNA. Translation: AAB88912.2.
BC089775 mRNA. Translation: AAH89775.1.
RefSeqiNP_620218.1. NM_138863.2.
UniGeneiRn.10656.

3D structure databases

ProteinModelPortaliP97584.
SMRiP97584. Positions 1-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4567058.
STRINGi10116.ENSRNOP00000020335.

Chemistry

BindingDBiP97584.
ChEMBLiCHEMBL4166.

PTM databases

iPTMnetiP97584.

Proteomic databases

PaxDbiP97584.
PRIDEiP97584.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020335; ENSRNOP00000020335; ENSRNOG00000015072.
GeneIDi192227.
KEGGirno:192227.
UCSCiRGD:621195. rat.

Organism-specific databases

CTDi22949.
RGDi621195. Ptgr1.

Phylogenomic databases

eggNOGiKOG1196. Eukaryota.
COG2130. LUCA.
GeneTreeiENSGT00390000009335.
HOGENOMiHOG000294663.
HOVERGENiHBG055024.
InParanoidiP97584.
KOiK13948.
OMAiYNRTGPP.
OrthoDBiEOG7JT6WX.
PhylomeDBiP97584.
TreeFamiTF324201.

Miscellaneous databases

NextBioi622836.
PROiP97584.

Gene expression databases

GenevisibleiP97584. RN.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR014190. B4_12hDH.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02825. B4_12hDH. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of cDNAs representing dithiolethione-responsive genes."
    Primiano T., Gastel J.A., Kensler T.W., Sutter T.R.
    Carcinogenesis 17:2297-2303(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY DITHIOLETHIONE.
    Strain: Fischer 344.
  2. Kensler T.W.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiPTGR1_RAT
AccessioniPrimary (citable) accession number: P97584
Secondary accession number(s): Q5EBD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.