Reviewed, UniProtKB/Swiss-Prot P97584 (PTGR1_RAT)

Names and origin

Protein names

Recommended name:
    Prostaglandin reductase 1
      Short name=PRG-1
    EC=1.3.1.-
Alternative name(s):
    NADP-dependent leukotriene B4 12-hydroxydehydrogenase
    EC=1.3.1.74
    15-oxoprostaglandin 13-reductase
    EC=1.3.1.48
    Dithiolethione-inducible gene 1 protein
      Short name=D3T-inducible gene 1 protein
      Short name=DIG-1

Gene names

Name:	Ptgr1
Synonyms:	Dig1, Ltb4dh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity towards PGE1, PGE2 and PGE2-alpha. Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4 By similarity.
Catalytic activity	n-alkanal + NAD(P)+ = alk-2-enal + NAD(P)H. 11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.
Subunit structure	Monomer or homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Induction	Up-regulated by 1,2-dithiole-3-thione (D3T). Ref.1
Sequence similarities	Belongs to the NADP-dependent oxidoreductase L4BD family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to toxin Ref.1 Inferred from expression pattern. Source: RGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	15-oxoprostaglandin 13-oxidase activity Inferred from electronic annotation. Source: EC 2-alkenal reductase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Prostaglandin reductase 1		PRO_0000218069
Regions
	Nucleotide binding	149 – 166	18	NADP Potential
	Compositional bias	250 – 257	8	Pro-rich
Sites
	Binding site	178	1	NADP By similarity
	Binding site	193	1	NADP By similarity
	Binding site	217	1	NADP By similarity
	Binding site	245	1	NADP By similarity
	Binding site	321	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97584-1 [UniParc]. Last modified May 1, 2000. Version 3. Checksum: 8E91165086A05BA9	FASTA	329	35,718
	10 20 30 40 50 60 MVQAKTWTLK KHFEGFPTDS NFELRTTELP PLNNGEVLLE ALFLSVDPYM RVAAKKLKEG 70 80 90 100 110 120 DSMMGEQVAR VVESKNSAFP TGTIVVALLG WTSHSISDGN GLRKLPAEWP DKLPLSLALG 130 140 150 160 170 180 TVGMPGLTAY FGLLDICGLK GGETVLVNAA AGAVGSVVGQ IAKLKGCKVV GTAGSDEKVA 190 200 210 220 230 240 YLKKLGFDVA FNYKTVKSLE EALRTASPDG YDCYFDNVGG EFSNTVILQM KTFGRIAICG 250 260 270 280 290 300 AISQYNRTGP CPPGPSPEVI IYQQLRMEGF IVTRWQGEVR QKALTDLMNW VSEGKIRYHE 310 320 YITEGFEKMP AAFMGMLKGD NLGKTIVKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of cDNAs representing dithiolethione-responsive genes." Primiano T., Gastel J.A., Kensler T.W., Sutter T.R. Carcinogenesis 17:2297-2303(1996) [PubMed: 8968041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY DITHIOLETHIONE. Strain: Fischer 344.
[2]	Kensler T.W. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U66322 mRNA. Translation: AAB88912.2. BC089775 mRNA. Translation: AAH89775.1.
IPI	IPI00203443.
RefSeq	NP_620218.1.
UniGene	Rn.10656
3D structure databases
SMR	P97584. Positions 1-329.
ModBase	Search...
Proteomic databases
PRIDE	P97584.
Genome annotation databases
Ensembl	ENSRNOT00000020335; ENSRNOP00000020335; ENSRNOG00000015072; Rattus norvegicus. [Genome view]
GeneID	192227.
KEGG	rno:192227.
UCSC	NM_138863. rat.
Organism-specific databases
CTD	192227.
RGD	621195. Ltb4dh.
Phylogenomic databases
eggNOG	roNOG16469.
HOVERGEN	P97584.
InParanoid	P97584.
PhylomeDB	P97584.
Enzyme and pathway databases
BRENDA	1.3.1.48. 248. 1.3.1.74. 248.
Gene expression databases
ArrayExpress	P97584.
Genevestigator	P97584.
GermOnline	ENSRNOG00000015072. Rattus norvegicus.
Family and domain databases
InterPro	IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR014190. B4_12hDH. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF00107. ADH_zinc_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02825. B4_12hDH. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	622836.

Entry information

Entry name

PTGR1_RAT

Accession

Primary (citable) accession number: P97584
Secondary accession number(s): Q5EBD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	May 1, 2000
Last modified:	February 9, 2010
This is version 68 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)