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P97577 (FEZ1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fasciculation and elongation protein zeta-1
Alternative name(s):
Zygin I
Zygin-1
Gene names
Name:Fez1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. May participate in the transport of mitochondria and other cargos along microtubules. Ref.4 Ref.5

Subunit structure

Homodimer. Interacts with UBE4B and SAP30L By similarity. Interacts with SCOC and ULK1; SCOC interferes with ULK1-binding to FEZ1 By similarity. Directly interacts with SCOC and UVRAG. Stabilizes the interaction between SCOC and UVRAG during amino acid starvation By similarity. Interacts with the NH2-terminal variable region (V1) of PKC zeta and weakly with that of PKC epsilon.

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cell membrane. Note: Colocalizes with both, alpha- and gamma-tubulin By similarity. Translocated from the plasma membrane to the cytoplasm by activation of the PKC zeta.

Tissue specificity

Brain.

Post-translational modification

Phosphorylated by protein kinase C zeta; which enhances interaction with UBE4B and polyubiquitination. Ref.1

Polyubiquitinated in a UBE4B-dependent manner; which does not lead to proteasomal degradation and may be important for neurogenic activity. Polyubiquitin linkage seems to be mainly through Lys-26 By similarity.

Sequence similarities

Belongs to the zygin family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Cytoplasm
Cytoskeleton
Membrane
Microtubule
   DomainCoiled coil
   PTMPhosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon guidance

Traceable author statement Ref.1. Source: RGD

cellular response to growth factor stimulus

Inferred from expression pattern Ref.5. Source: RGD

establishment of mitochondrion localization

Inferred from mutant phenotype Ref.5. Source: RGD

positive regulation of neuron differentiation

Inferred from direct assay Ref.1. Source: RGD

positive regulation of neuron projection development

Inferred from mutant phenotype Ref.5. Source: RGD

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentaxon

Inferred from direct assay PubMed 19199094. Source: RGD

cell projection

Inferred from direct assay PubMed 19199094. Source: RGD

centrosome

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from direct assay PubMed 19199094. Source: RGD

dendrite

Inferred from direct assay PubMed 19199094. Source: RGD

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from direct assay Ref.1. Source: RGD

   Molecular_functionprotein N-terminus binding

Inferred from physical interaction Ref.1. Source: RGD

protein binding

Inferred from physical interaction Ref.5. Source: RGD

protein kinase C binding

Inferred from physical interaction Ref.1. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393Fasciculation and elongation protein zeta-1
PRO_0000189527

Regions

Coiled coil231 – 29969 Potential
Compositional bias95 – 984Poly-Glu

Amino acid modifications

Modified residue581Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P97577 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 62F1F9552E326C55

FASTA39345,244
        10         20         30         40         50         60 
MEAPLVSLDE EFEDIRPCCT EDPEEKPQSL YGTSPHHLED PSLSELENFS SEIISFKSME 

        70         80         90        100        110        120 
DLVNEFDEKL NVCFRNYNAK TENLAPVKNQ LQIQEEEETL RDEEVWDALT DNYIPSLSED 

       130        140        150        160        170        180 
WRDPNIEALN GNSSDTEIHE KEEEDEFIEK SENDSGINEE PLLTADQVIE EIEEMMQNSP 

       190        200        210        220        230        240 
DPEEEVEVLE EEDGGEISSQ ADSVLLQEMQ ALTQTFNNNW SYEGLRHMSG SELTELLDQV 

       250        260        270        280        290        300 
EGAIRDFSEE LVHQLARRDE LEFEKEVKNS FITVLIEVQN KQKEQRELMK KRRKEKGLSL 

       310        320        330        340        350        360 
QSSRIEKGNQ MPLKRFSMEG ISNILQSGIR QTFGSSGADR QYLNTVIPYE KKSSPPSVED 

       370        380        390 
LQMLTNILFA MKEDNEKVPT LLTDYILKVL CPT 

« Hide

References

« Hide 'large scale' references
[1]"Mammalian homologue of the Caenorhabditis elegans UNC-76 protein involved in axonal outgrowth is a protein kinase C zeta-interacting protein."
Kuroda S., Nakagawa N., Tokunaga C., Tatematsu K., Tanizawa K.
J. Cell Biol. 144:403-411(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION.
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Zigins: a family of synaptotagmin-interacting proteins related to unc-76."
Sugita S., von Poser C., Rosahl T.W., Hata Y., Suedhof T.C.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Fasciculation and elongation protein zeta-1 (FEZ1) participates in the polarization of hippocampal neuron by controlling the mitochondrial motility."
Ikuta J., Maturana A., Fujita T., Okajima T., Tatematsu K., Tanizawa K., Kuroda S.
Biochem. Biophys. Res. Commun. 353:127-132(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Axonal guidance protein FEZ1 associates with tubulin and kinesin motor protein to transport mitochondria in neurites of NGF-stimulated PC12 cells."
Fujita T., Maturana A.D., Ikuta J., Hamada J., Walchli S., Suzuki T., Sawa H., Wooten M.W., Okajima T., Tatematsu K., Tanizawa K., Kuroda S.
Biochem. Biophys. Res. Commun. 361:605-610(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U48249 mRNA. Translation: AAC71216.2.
U63740 mRNA. Translation: AAB40630.1.
BC087740 mRNA. Translation: AAH87740.1.
RefSeqNP_112328.1. NM_031066.1.
XP_006242873.1. XM_006242811.1.
UniGeneRn.5468.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid249603. 1 interaction.
STRING10116.ENSRNOP00000008285.

Proteomic databases

PaxDbP97577.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000008285; ENSRNOP00000008285; ENSRNOG00000006075.
GeneID81730.
KEGGrno:81730.
UCSCRGD:619708. rat.

Organism-specific databases

CTD9638.
RGD619708. Fez1.

Phylogenomic databases

eggNOGNOG273183.
GeneTreeENSGT00390000017627.
HOGENOMHOG000019494.
HOVERGENHBG005657.
InParanoidP97577.
OMAEMMENSP.
OrthoDBEOG7CCBRQ.
PhylomeDBP97577.
TreeFamTF313128.

Gene expression databases

GenevestigatorP97577.

Family and domain databases

InterProIPR011680. FEZ.
IPR015642. FEZ_1.
[Graphical view]
PANTHERPTHR12394. PTHR12394. 1 hit.
PTHR12394:SF4. PTHR12394:SF4. 1 hit.
PfamPF07763. FEZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio615396.
PROP97577.

Entry information

Entry nameFEZ1_RAT
AccessionPrimary (citable) accession number: P97577
Secondary accession number(s): Q62922
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 1997
Last modified: June 11, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families