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Protein

Calpain-1 catalytic subunit

Gene

Capn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+By similarityNote: Binds 4 Ca2+ ions.By similarity

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115By similarity1
Active sitei272By similarity1
Active sitei296By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi99 – 10618
Calcium bindingi302 – 3332Add BLAST32
Calcium bindingi597 – 6083PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi627 – 6384PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
  • calcium ion binding Source: RGD
  • cytoskeletal protein binding Source: RGD

GO - Biological processi

  • protein autoprocessing Source: RGD
  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.52. 5301.

Protein family/group databases

MEROPSiC02.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-1 catalytic subunit (EC:3.4.22.52)
Alternative name(s):
Calcium-activated neutral proteinase 1
Short name:
CANP 1
Calpain mu-type
Calpain-1 large subunit
Micromolar-calpain
Short name:
muCANP
Gene namesi
Name:Capn1
Synonyms:Cls1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2267. Capn1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3747.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002077002 – 713Calpain-1 catalytic subunitAdd BLAST712

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei354PhosphothreonineBy similarity1

Post-translational modificationi

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei15 – 16Cleavage; for 78 kDa formBy similarity2
Sitei27 – 28Cleavage; for 75 kDa formBy similarity2

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein

Proteomic databases

PaxDbiP97571.
PRIDEiP97571.

PTM databases

iPTMnetiP97571.
PhosphoSitePlusiP97571.

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).1 Publication

GO - Molecular functioni

  • cytoskeletal protein binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028431.

Chemistry databases

BindingDBiP97571.

Structurei

Secondary structure

1713
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi37 – 39Combined sources3
Helixi42 – 51Combined sources10
Helixi65 – 68Combined sources4
Beta strandi70 – 76Combined sources7
Helixi78 – 82Combined sources5
Beta strandi84 – 86Combined sources3
Helixi88 – 91Combined sources4
Beta strandi99 – 102Combined sources4
Helixi104 – 106Combined sources3
Beta strandi111 – 113Combined sources3
Helixi115 – 124Combined sources10
Helixi128 – 134Combined sources7
Beta strandi147 – 155Combined sources9
Beta strandi158 – 166Combined sources9
Beta strandi168 – 171Combined sources4
Beta strandi174 – 177Combined sources4
Beta strandi181 – 183Combined sources3
Helixi187 – 199Combined sources13
Beta strandi200 – 202Combined sources3
Helixi203 – 205Combined sources3
Beta strandi206 – 208Combined sources3
Helixi210 – 216Combined sources7
Beta strandi221 – 226Combined sources6
Helixi227 – 229Combined sources3
Helixi234 – 244Combined sources11
Beta strandi247 – 251Combined sources5
Beta strandi255 – 258Combined sources4
Beta strandi261 – 263Combined sources3
Beta strandi269 – 271Combined sources3
Beta strandi274 – 284Combined sources11
Beta strandi287 – 295Combined sources9
Helixi312 – 316Combined sources5
Helixi319 – 325Combined sources7
Beta strandi331 – 337Combined sources7
Helixi338 – 344Combined sources7
Beta strandi347 – 352Combined sources6
Beta strandi370 – 375Combined sources6
Turni377 – 379Combined sources3
Turni388 – 390Combined sources3
Helixi391 – 393Combined sources3
Beta strandi398 – 401Combined sources4
Beta strandi418 – 425Combined sources8
Beta strandi441 – 447Combined sources7
Helixi461 – 464Combined sources4
Beta strandi477 – 480Combined sources4
Beta strandi482 – 487Combined sources6
Beta strandi490 – 503Combined sources14
Beta strandi506 – 516Combined sources11
Beta strandi518 – 520Combined sources3
Beta strandi528 – 530Combined sources3
Beta strandi556 – 559Combined sources4
Turni565 – 569Combined sources5
Helixi586 – 596Combined sources11
Beta strandi606 – 608Combined sources3
Helixi609 – 623Combined sources15
Helixi624 – 626Combined sources3
Helixi636 – 645Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KXRX-ray2.07A/B27-356[»]
1QXPX-ray2.80A/B60-647[»]
1TL9X-ray1.80A27-356[»]
1TLOX-ray1.90A27-356[»]
2G8EX-ray2.25A27-356[»]
2G8JX-ray1.61A27-356[»]
2NQGX-ray2.04A27-356[»]
2NQIX-ray2.04A27-356[»]
2R9CX-ray1.80A27-356[»]
2R9FX-ray1.60A27-356[»]
ProteinModelPortaliP97571.
SMRiP97571.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP97571.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 354Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini540 – 575EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini584 – 617EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini614 – 649EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini679 – 713EF-hand 4PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni355 – 525Domain IIIAdd BLAST171
Regioni526 – 541LinkerAdd BLAST16
Regioni542 – 712Domain IVAdd BLAST171

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiP97571.
KOiK01367.
PhylomeDBiP97571.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029643. CAPN1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF284. PTHR10183:SF284. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97571-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEELITPVY CTGVSAQVQK QRDKELGLGR HENAIKYLGQ DYENLRARCL
60 70 80 90 100
QNGVLFQDDA FPPVSHSLGF KELGPNSSKT YGIKWKRPTE LLSNPQFIVD
110 120 130 140 150
GATRTDICQG ALGDCWLLAA IASLTLNETI LHRVVPYGQS FQEGYAGIFH
160 170 180 190 200
FQLWQFGEWV DVVVDDLLPT KDGKLVFVHS AQGNEFWSAL LEKAYAKVNG
210 220 230 240 250
SYEALSGGCT SEAFEDFTGG VTEWYDLQKA PSDLYQIILK ALERGSLLGC
260 270 280 290 300
SINISDIRDL EAITFKNLVR GHAYSVTDAK QVTYQGQRVN LIRMRNPWGE
310 320 330 340 350
VEWKGPWSDN SYEWNKVDPY EREQLRVKME DGEFWMSFRD FIREFTKLEI
360 370 380 390 400
CNLTPDALKS RTLRNWNTTF YEGTWRRGST AGGCRNYPAT FWVNPQFKIR
410 420 430 440 450
LEEVDDADDY DSRESGCSFL LALMQKHRRR ERRFGRDMET IGFAVYQVPR
460 470 480 490 500
ELAGQPVHLK RDFFLANASR AQSEHFINLR EVSNRIRLPP GEYIVVPSTF
510 520 530 540 550
EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE EIDDNFKTLF
560 570 580 590 600
SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG
610 620 630 640 650
NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL
660 670 680 690 700
NKKLHELIIT RYSEPDLAVD FDNFVCCLVR LETMFRFFKI LDTDLDGVVT
710
FDLFKWLQLT MFA
Length:713
Mass (Da):82,119
Last modified:May 1, 1997 - v1
Checksum:i6E664600B0EFAEBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53858 mRNA. Translation: AAC53001.1.
BC061880 mRNA. Translation: AAH61880.1.
RefSeqiNP_062025.1. NM_019152.2.
UniGeneiRn.6037.

Genome annotation databases

GeneIDi29153.
KEGGirno:29153.
UCSCiRGD:2267. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53858 mRNA. Translation: AAC53001.1.
BC061880 mRNA. Translation: AAH61880.1.
RefSeqiNP_062025.1. NM_019152.2.
UniGeneiRn.6037.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KXRX-ray2.07A/B27-356[»]
1QXPX-ray2.80A/B60-647[»]
1TL9X-ray1.80A27-356[»]
1TLOX-ray1.90A27-356[»]
2G8EX-ray2.25A27-356[»]
2G8JX-ray1.61A27-356[»]
2NQGX-ray2.04A27-356[»]
2NQIX-ray2.04A27-356[»]
2R9CX-ray1.80A27-356[»]
2R9FX-ray1.60A27-356[»]
ProteinModelPortaliP97571.
SMRiP97571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028431.

Chemistry databases

BindingDBiP97571.
ChEMBLiCHEMBL3747.

Protein family/group databases

MEROPSiC02.001.

PTM databases

iPTMnetiP97571.
PhosphoSitePlusiP97571.

Proteomic databases

PaxDbiP97571.
PRIDEiP97571.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29153.
KEGGirno:29153.
UCSCiRGD:2267. rat.

Organism-specific databases

CTDi823.
RGDi2267. Capn1.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiP97571.
KOiK01367.
PhylomeDBiP97571.

Enzyme and pathway databases

BRENDAi3.4.22.52. 5301.

Miscellaneous databases

EvolutionaryTraceiP97571.
PROiP97571.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029643. CAPN1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF284. PTHR10183:SF284. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN1_RAT
AccessioniPrimary (citable) accession number: P97571
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.