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Protein

85/88 kDa calcium-independent phospholipase A2

Gene

Pla2g6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F-actin polymerization at the pseudopods (By similarity).By similarity

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by calcium-activated calmodulin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei520NucleophilePROSITE-ProRule annotation1
Active sitei653Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP-dependent protein binding Source: RGD
  • calcium-independent phospholipase A2 activity Source: RGD
  • phospholipase A2 activity Source: Reactome
  • protein kinase binding Source: RGD

GO - Biological processi

  • antibacterial humoral response Source: Ensembl
  • cardiolipin acyl-chain remodeling Source: Reactome
  • cardiolipin biosynthetic process Source: Ensembl
  • chemotaxis Source: UniProtKB-KW
  • lipid catabolic process Source: UniProtKB-KW
  • maternal process involved in female pregnancy Source: RGD
  • memory Source: RGD
  • negative regulation of synaptic transmission, glutamatergic Source: RGD
  • positive regulation of arachidonic acid secretion Source: RGD
  • positive regulation of ceramide biosynthetic process Source: RGD
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of exocytosis Source: RGD
  • positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: RGD
  • positive regulation of protein kinase C signaling Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • positive regulation of release of cytochrome c from mitochondria Source: RGD
  • positive regulation of vasodilation Source: RGD
  • regulation of store-operated calcium channel activity Source: RGD
  • response to endoplasmic reticulum stress Source: RGD
  • urinary bladder smooth muscle contraction Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Chemotaxis, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-RNO-1482788. Acyl chain remodelling of PC.
R-RNO-1482798. Acyl chain remodeling of CL.
R-RNO-1482839. Acyl chain remodelling of PE.
R-RNO-2029485. Role of phospholipids in phagocytosis.
R-RNO-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
85/88 kDa calcium-independent phospholipase A2 (EC:3.1.1.4)
Short name:
CaI-PLA2
Alternative name(s):
Group VI phospholipase A2
Short name:
GVI PLA2
Intracellular membrane-associated calcium-independent phospholipase A2 beta
Short name:
iPLA2-beta
Patatin-like phospholipase domain-containing protein 9
Short name:
PNPLA9
Gene namesi
Name:Pla2g6
Synonyms:Pnpla9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi628867. Pla2g6.

Subcellular locationi

Isoform Long :
Isoform Short :

GO - Cellular componenti

  • cytosol Source: RGD
  • extracellular space Source: Ensembl
  • microtubule organizing center Source: Ensembl
  • mitochondrial inner membrane Source: Reactome
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075318.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670391 – 80785/88 kDa calcium-independent phospholipase A2Add BLAST807

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP97570.
PRIDEiP97570.

PTM databases

iPTMnetiP97570.
PhosphoSitePlusiP97570.

Expressioni

Tissue specificityi

Found in brain, lung, spleen, kidney, liver, heart and skeletal muscle.

Gene expression databases

BgeeiENSRNOG00000012295.
GenevisibleiP97570. RN.

Interactioni

GO - Molecular functioni

  • ATP-dependent protein binding Source: RGD
  • protein kinase binding Source: RGD

Protein-protein interaction databases

IntActiP97570. 1 interactor.
STRINGi10116.ENSRNOP00000017104.

Structurei

3D structure databases

ProteinModelPortaliP97570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati150 – 180ANK 1Add BLAST31
Repeati184 – 214ANK 2Add BLAST31
Repeati218 – 247ANK 3Add BLAST30
Repeati250 – 280ANK 4Add BLAST31
Repeati285 – 311ANK 5Add BLAST27
Repeati315 – 344ANK 6Add BLAST30
Repeati348 – 377ANK 7Add BLAST30
Domaini482 – 666PNPLAPROSITE-ProRule annotationAdd BLAST185

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni678 – 687Calmodulin-binding10
Regioni749 – 760Calmodulin-bindingAdd BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi486 – 491GXGXXGPROSITE-ProRule annotation6
Motifi518 – 522GXSXGPROSITE-ProRule annotation5
Motifi653 – 655DGA/GPROSITE-ProRule annotation3

Sequence similaritiesi

Contains 7 ANK repeats.PROSITE-ProRule annotation
Contains 1 PNPLA (patatin-like phospholipase) domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0513. Eukaryota.
COG0666. LUCA.
COG3621. LUCA.
GeneTreeiENSGT00530000063645.
HOGENOMiHOG000013092.
HOVERGENiHBG053482.
InParanoidiP97570.
KOiK16343.
OMAiIFRTHEI.
OrthoDBiEOG091G042U.
PhylomeDBiP97570.
TreeFamiTF319230.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR002641. Patatin/PLipase_A2-rel.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF13606. Ank_3. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
PS51635. PNPLA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P97570-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQFFGRLVNT LSSVTNLFSN PFRVKEVSLA DYASSERVRE EGQLILLQNA
60 70 80 90 100
SNRTWDCVLV SPRNPQSGFR LFQLESEADA LVNFQQYSSQ LPPFYESSVQ
110 120 130 140 150
VLHVEVLQHL TDLIRNHPSW TVTHLAVELG IRECFHHSRI ISCANSTENE
160 170 180 190 200
EGCTPLHLAC RKGDSEILVE LVQYCHAQMD VTDNKGETAF HYAVQGDNPQ
210 220 230 240 250
VLQLLGKNAS AGLNQVNNQG LTPLHLACQM GKQEMVRVLL LCNARCNIMG
260 270 280 290 300
PGGFPIHTAM KFSQKGCAEM IISMDSNQIH SKDPRYGASP LHWAKNAEMA
310 320 330 340 350
RMLLKRGCDV DSTSASGNTA LHVAVTRNRF DCVMVLLTYG ANAGARGEHG
360 370 380 390 400
NTPLHLAMSK DNMEMVKALI VFGAEVDTPN DFGETPAFIA SKISKLITRK
410 420 430 440 450
ALLTLLKTVG ADYHFPFIQG VSTEQSSAAG PHPFFSLDRT QPPTISLNNL
460 470 480 490 500
ELQDLMPVSR ARKPAFILSS MRDEKRSHDH LLCLDGGGVK GLVIIQLLIA
510 520 530 540 550
IEKASGVATK DLFDWVAGTS TGGILALAIL HSKSMAYMRG VYFRMKDEVF
560 570 580 590 600
RGSRPYESGP LEEFLKREFG EHTKMTDVKK PKVMLTGTLS DRQPAELHLF
610 620 630 640 650
RNYDAPEAVR EPRCTPNINL KPPTQPADQL VWRAARSSGA APTYFRPNGR
660 670 680 690 700
FLDGGLLANN PTLDAMTEIH EYNQDMIRKG QGNKVKKLSI VVSLGTGKSP
710 720 730 740 750
QVPVTCVDVF RPSNPWELAK TVFGAKELGK MVVDCCTDPD GRAVDRARAW
760 770 780 790 800
CEMVGIQYFR LNPQLGSDIM LDEVSDAVLV NALWETEVYI YEHREEFQKL

VQLLLSP
Length:807
Mass (Da):89,556
Last modified:October 31, 2012 - v2
Checksum:i1B9018AE1B2D252F
GO
Isoform Short (identifier: P97570-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     396-451: LITRKALLTLLKTVGADYHFPFIQGVSTEQSSAAGPHPFFSLDRTQPPTISLNNLE → Q

Show »
Length:752
Mass (Da):83,562
Checksum:iE622616FB108FDA0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24V → A in AAC53136 (PubMed:9111008).Curated1
Sequence conflicti58V → D in AAC53136 (PubMed:9111008).Curated1
Sequence conflicti68G → D in AAC53136 (PubMed:9111008).Curated1
Sequence conflicti80A → V in AAC53136 (PubMed:9111008).Curated1
Sequence conflicti99V → E in AAC53136 (PubMed:9111008).Curated1
Sequence conflicti109Missing in AAC53136 (PubMed:9111008).Curated1
Sequence conflicti142S → T in AAC53136 (PubMed:9111008).Curated1
Sequence conflicti477S → T in AAC53136 (PubMed:9111008).Curated1
Sequence conflicti793H → Y in AAC53136 (PubMed:9111008).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044365396 – 451LITRK…LNNLE → Q in isoform Short. 1 PublicationAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51898 mRNA. Translation: AAC53136.1.
AABR06052011 Genomic DNA. No translation available.
CH473950 Genomic DNA. Translation: EDM15808.1.
CH473950 Genomic DNA. Translation: EDM15809.1.
BC081916 mRNA. Translation: AAH81916.1.
RefSeqiNP_001005560.1. NM_001005560.1. [P97570-1]
NP_001257725.1. NM_001270796.1. [P97570-2]
UniGeneiRn.44692.

Genome annotation databases

EnsembliENSRNOT00000016827; ENSRNOP00000016827; ENSRNOG00000012295. [P97570-2]
ENSRNOT00000017108; ENSRNOP00000017104; ENSRNOG00000012295. [P97570-1]
ENSRNOT00000087344; ENSRNOP00000072104; ENSRNOG00000012295. [P97570-1]
ENSRNOT00000090565; ENSRNOP00000075311; ENSRNOG00000012295. [P97570-2]
GeneIDi360426.
KEGGirno:360426.
UCSCiRGD:628867. rat. [P97570-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51898 mRNA. Translation: AAC53136.1.
AABR06052011 Genomic DNA. No translation available.
CH473950 Genomic DNA. Translation: EDM15808.1.
CH473950 Genomic DNA. Translation: EDM15809.1.
BC081916 mRNA. Translation: AAH81916.1.
RefSeqiNP_001005560.1. NM_001005560.1. [P97570-1]
NP_001257725.1. NM_001270796.1. [P97570-2]
UniGeneiRn.44692.

3D structure databases

ProteinModelPortaliP97570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP97570. 1 interactor.
STRINGi10116.ENSRNOP00000017104.

Chemistry databases

ChEMBLiCHEMBL1075318.

PTM databases

iPTMnetiP97570.
PhosphoSitePlusiP97570.

Proteomic databases

PaxDbiP97570.
PRIDEiP97570.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016827; ENSRNOP00000016827; ENSRNOG00000012295. [P97570-2]
ENSRNOT00000017108; ENSRNOP00000017104; ENSRNOG00000012295. [P97570-1]
ENSRNOT00000087344; ENSRNOP00000072104; ENSRNOG00000012295. [P97570-1]
ENSRNOT00000090565; ENSRNOP00000075311; ENSRNOG00000012295. [P97570-2]
GeneIDi360426.
KEGGirno:360426.
UCSCiRGD:628867. rat. [P97570-1]

Organism-specific databases

CTDi8398.
RGDi628867. Pla2g6.

Phylogenomic databases

eggNOGiKOG0513. Eukaryota.
COG0666. LUCA.
COG3621. LUCA.
GeneTreeiENSGT00530000063645.
HOGENOMiHOG000013092.
HOVERGENiHBG053482.
InParanoidiP97570.
KOiK16343.
OMAiIFRTHEI.
OrthoDBiEOG091G042U.
PhylomeDBiP97570.
TreeFamiTF319230.

Enzyme and pathway databases

ReactomeiR-RNO-1482788. Acyl chain remodelling of PC.
R-RNO-1482798. Acyl chain remodeling of CL.
R-RNO-1482839. Acyl chain remodelling of PE.
R-RNO-2029485. Role of phospholipids in phagocytosis.
R-RNO-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiP97570.

Gene expression databases

BgeeiENSRNOG00000012295.
GenevisibleiP97570. RN.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR002641. Patatin/PLipase_A2-rel.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF13606. Ank_3. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
PS51635. PNPLA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLPL9_RAT
AccessioniPrimary (citable) accession number: P97570
Secondary accession number(s): G3V7M8, Q66HD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 31, 2012
Last modified: November 30, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.