Reviewed, UniProtKB/Swiss-Prot P97564 (GPAT1_RAT)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase 1, mitochondrial
      Short name=GPAT-1
    EC=2.3.1.15

Gene names

Name:	Gpam
Synonyms:	Gpat1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis By similarity.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. Ref.1
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Mitochondrion outer membrane; Multi-pass membrane protein Ref.2.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transit peptide Transmembrane
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycerol-3-phosphate O-acyltransferase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 828		Glycerol-3-phosphate acyltransferase 1, mitochondrial		PRO_0000024692
Regions
	Topological domain	? – 471		Mitochondrial intermembrane Potential
	Transmembrane	472 – 494	23	Potential
	Topological domain	495 – 574	80	Cytoplasmic Potential
	Transmembrane	575 – 593	19	Potential
	Topological domain	594 – 828	235	Mitochondrial intermembrane Potential
	Motif	230 – 235	6	HXXXXD motif
Amino acid modifications
	Modified residue	111	1	Phosphotyrosine By similarity
	Modified residue	695	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	37	1	C → F Ref.2
	Sequence conflict	85	1	S → P in AAB39470. Ref.2
	Sequence conflict	300	1	I → V in AAB39470. Ref.2
	Sequence conflict	331	1	L → V in AAB39470. Ref.2
	Sequence conflict	472 – 474	3	ILF → NLL in AAB39470. Ref.2
	Sequence conflict	497	1	R → W in AAB39470. Ref.2
	Sequence conflict	602 – 606	5	SAGGL → LPEP in AAB39470. Ref.2
	Sequence conflict	644	1	Q → H in AAB39470. Ref.2
	Sequence conflict	744	1	G → A in AAB39470. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P97564-1 [UniParc]. Last modified December 15, 1998. Version 3. Checksum: ACA4A087E7DEB12C	FASTA	828	93,715
	10 20 30 40 50 60 MEESSVTIGT IDVSYLPNSS EYSLGRCKHT NEDWVDCGFK PTFFRSATLK WKESLMSRKR 70 80 90 100 110 120 PFVGRCCYSC TPQSWERFFN PSIPSLGLRN VIYINETHTR HRGWLARRLS YILFVQERDV 130 140 150 160 170 180 HKGMFATSIT DNVLNSSRVQ EAIAEVAAEL NPDGSAQQQS KAIQKVKRKA RKILQEMVAT 190 200 210 220 230 240 VSPGMIRLTG WVLLKLFNSF FWNIQIHKGQ LEMVKAATET NLPLLFLPVH RSHIDYLLLT 250 260 270 280 290 300 FILFCHNIKA PYIASGNNLN IPIFSTLIHK LGGFFIRRRL DETPDGRKDI LYRALLHGHI 310 320 330 340 350 360 VELLRQQQFL EIFLEGTRSR SGKTSCARAG LLSVVVDTLS SNTIPDILVI PVGISYDRII 370 380 390 400 410 420 EGHYNGEQLG KPKKNESLWS VARGVIRMLR KNYGYVRVDF AQPFSLKEYL EGQSQKPVSA 430 440 450 460 470 480 PLSLEQALLP AILPSRPDAA AAEHEDMSSN ESRNAADEAF RRRLIANLAE HILFTASKSC 490 500 510 520 530 540 AIMSTHIVAC LLLYRHRQGI HLSTLVEDFF VMKEEVLARD FDLGFSGNSE DVVMHAIQLL 550 560 570 580 590 600 GNCVTITHTS RKDEFFITPS TTVPSVFELN FYSNGVLHVF IMEAIIACSI YAVQNKRGSG 610 620 630 640 650 660 GSAGGLGNLI SQEQLVRKAA SLCYLLSNEG TISLPCQTFY QVCQETVGKF IQYGILTVAE 670 680 690 700 710 720 QDDQEDVSPG LAEQQWNKKL PEPLNWRSDE EDEDSDFGEE QRDCYLKVSQ AKEHQQFITF 730 740 750 760 770 780 LQRLLGPLLE AYSSAAIFVH TFRGPVPESE YLQKLHRYLL TRTERNVAVY AESATYCLVK 790 800 810 820 NAVKMFKDIG VFKETKQKRA SVLELSTTFL PQGSRQKLLE YILSFVVL

References

[1]	"Rat sn-glycerol-3-phosphate acyltransferase: molecular cloning and characterization of the cDNA and expressed protein." Bhat B.G., Wang P., Kim J.-H., Black T.M., Lewin T.M., Fiedorek F.T. Jr., Coleman R.A. Biochim. Biophys. Acta 1439:415-423(1999) [PubMed: 10446428] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY. Tissue: Liver.
[2]	"Identification of two transmembrane regions and a cytosolic domain of rat mitochondrial glycerophosphate acyltransferase." Balija V.S., Chakraborty T.R., Nikonov A.V., Morimoto T., Haldar D. J. Biol. Chem. 275:31668-31673(2000) [PubMed: 10924502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TOPOLOGY. Strain: Sprague-Dawley. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF021348 mRNA. Translation: AAB71605.1. U36771 mRNA. Translation: AAB39470.2. Different initiation.
IPI	IPI00205564.
RefSeq	NP_058970.1.
UniGene	Rn.44456
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P97564.
PTM databases
PhosphoSite	P97564.
Proteomic databases
PRIDE	P97564.
Genome annotation databases
Ensembl	ENSRNOT00000047903; ENSRNOP00000050050; ENSRNOG00000015124; Rattus norvegicus. [Genome view]
GeneID	29653.
KEGG	rno:29653.
UCSC	NM_017274. rat.
Organism-specific databases
CTD	29653.
RGD	61847. Gpam.
Phylogenomic databases
eggNOG	roNOG06258.
HOVERGEN	P97564.
InParanoid	P97564.
Enzyme and pathway databases
BRENDA	2.3.1.15. 248.
Gene expression databases
ArrayExpress	P97564.
Genevestigator	P97564.
GermOnline	ENSRNOG00000015124. Rattus norvegicus.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	609941.

Entry information

Entry name

GPAT1_RAT

Accession

Primary (citable) accession number: P97564
Secondary accession number(s): O35349, P97565, P97566

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	February 9, 2010
This is version 76 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)