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P97553

- EFNA1_RAT

UniProt

P97553 - EFNA1_RAT

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Protein
Ephrin-A1
Gene
Efna1, Epgl1, Lerk1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis By similarity.

GO - Molecular functioni

  1. ephrin receptor binding Source: RefGenome

GO - Biological processi

  1. activation of MAPK activity Source: Ensembl
  2. angiogenesis Source: RGD
  3. aortic valve morphogenesis Source: Ensembl
  4. axon guidance Source: RefGenome
  5. cell migration Source: Ensembl
  6. endocardial cushion to mesenchymal transition involved in heart valve formation Source: Ensembl
  7. ephrin receptor signaling pathway Source: UniProtKB
  8. mitral valve morphogenesis Source: Ensembl
  9. negative regulation of dendritic spine morphogenesis Source: UniProtKB
  10. negative regulation of epithelial to mesenchymal transition Source: Ensembl
  11. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  12. notochord formation Source: Ensembl
  13. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  14. regulation of angiogenesis Source: Ensembl
  15. regulation of axonogenesis Source: Ensembl
  16. regulation of blood vessel endothelial cell migration Source: Ensembl
  17. regulation of cell adhesion mediated by integrin Source: Ensembl
  18. regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
  19. substrate adhesion-dependent cell spreading Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Angiogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin-A1
Alternative name(s):
EPH-related receptor tyrosine kinase ligand 1
Short name:
LERK-1
Immediate early response protein B61
Cleaved into the following chain:
Gene namesi
Name:Efna1
Synonyms:Epgl1, Lerk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi620388. Efna1.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of plasma membrane Source: RefGenome
  2. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed prediction
Add
BLAST
Chaini18 – 182165Ephrin-A1
PRO_0000008357Add
BLAST
Chaini18 – ?Ephrin-A1, secreted formPRO_0000389633
Propeptidei183 – 20523Removed in mature form Reviewed prediction
PRO_0000008358Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi26 – 261N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi51 ↔ 92 By similarity
Disulfide bondi80 ↔ 140 By similarity
Lipidationi182 – 1821GPI-anchor amidated serine Reviewed prediction

Post-translational modificationi

Undergoes proteolysis by a metalloprotease to give rise to a soluble monomeric form By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Expressioni

Gene expression databases

GenevestigatoriP97553.

Interactioni

Subunit structurei

Monomer. Homodimer. Forms heterodimers with EPHA2. Binds to the receptor tyrosine kinases EPHA2, EPHA3, EPHA4, EPHA5, EPHA6 and EPHA7. Also binds with low affinity to EPHA1 By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027920.

Structurei

3D structure databases

ProteinModelPortaliP97553.
SMRiP97553. Positions 18-149.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 151134Ephrin RBD
Add
BLAST

Sequence similaritiesi

Belongs to the ephrin family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG296825.
GeneTreeiENSGT00390000015107.
HOGENOMiHOG000234373.
HOVERGENiHBG051447.
InParanoidiP97553.
KOiK05462.
OMAiPIHHQED.
OrthoDBiEOG70W3FD.
PhylomeDBiP97553.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view]
PANTHERiPTHR11304. PTHR11304. 1 hit.
PfamiPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSiPR01347. EPHRIN.
ProDomiPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97553-1 [UniParc]FASTAAdd to Basket

« Hide

MEFLWAPLLG LCCSLAAADR HIVFWNSSNP KFREEDYTVH VQLNDYLDII    50
CPHYEDDSVA DAAMERYTLY MVEHQEYVTC EPQSKDQVRW KCNQPSAKHG 100
PEKLSEKFQR FTPFTLGKEF KEGHSYYYIS KPIYHQETQC LKLKVTVNGK 150
ITHSPHAHAN PQEKRLQADD PEVQVLHSIG HSAAPRLFPL VWAVLLLPLL 200
LLQTQ 205
Length:205
Mass (Da):23,763
Last modified:August 30, 2005 - v2
Checksum:iE9ED3F73AE7FC4CA
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181A → V in BAA07242. 1 Publication
Sequence conflicti68 – 681T → S in BAA07242. 1 Publication
Sequence conflicti90 – 901W → V in BAA07242. 1 Publication
Sequence conflicti159 – 1591A → V in BAA07242. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38056 mRNA. Translation: BAA07242.1.
BC070514 mRNA. Translation: AAH70514.1.
RefSeqiNP_446051.2. NM_053599.2.
UniGeneiRn.8427.

Genome annotation databases

EnsembliENSRNOT00000027920; ENSRNOP00000027920; ENSRNOG00000020573.
GeneIDi94268.
KEGGirno:94268.
UCSCiRGD:620388. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38056 mRNA. Translation: BAA07242.1 .
BC070514 mRNA. Translation: AAH70514.1 .
RefSeqi NP_446051.2. NM_053599.2.
UniGenei Rn.8427.

3D structure databases

ProteinModelPortali P97553.
SMRi P97553. Positions 18-149.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000027920.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000027920 ; ENSRNOP00000027920 ; ENSRNOG00000020573 .
GeneIDi 94268.
KEGGi rno:94268.
UCSCi RGD:620388. rat.

Organism-specific databases

CTDi 1942.
RGDi 620388. Efna1.

Phylogenomic databases

eggNOGi NOG296825.
GeneTreei ENSGT00390000015107.
HOGENOMi HOG000234373.
HOVERGENi HBG051447.
InParanoidi P97553.
KOi K05462.
OMAi PIHHQED.
OrthoDBi EOG70W3FD.
PhylomeDBi P97553.

Miscellaneous databases

NextBioi 617895.
PROi P97553.

Gene expression databases

Genevestigatori P97553.

Family and domain databases

Gene3Di 2.60.40.420. 1 hit.
InterProi IPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view ]
PANTHERi PTHR11304. PTHR11304. 1 hit.
Pfami PF00812. Ephrin. 1 hit.
[Graphical view ]
PRINTSi PR01347. EPHRIN.
ProDomi PD002533. Ephrin. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF49503. SSF49503. 1 hit.
PROSITEi PS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of rat and mouse B61 gene: implications on organogenesis."
    Takahashi H., Ikeda T.
    Oncogene 11:879-883(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiEFNA1_RAT
AccessioniPrimary (citable) accession number: P97553
Secondary accession number(s): Q6NS29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 30, 2005
Last modified: April 16, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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