P97553 (EFNA1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ephrin-A1

Alternative name(s):
EPH-related receptor tyrosine kinase ligand 1
Short name=LERK-1
Immediate early response protein B61

Cleaved into the following chain:

1. Ephrin-A1, secreted form

Gene names

Name:	Efna1
Synonyms:	Epgl1, Lerk1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis By similarity.
Subunit structure	Monomer. Homodimer. Forms heterodimers with EPHA2. Binds to the receptor tyrosine kinases EPHA2, EPHA3, EPHA4, EPHA5, EPHA6 and EPHA7. Also binds with low affinity to EPHA1 By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity. Ephrin-A1, secreted form: Secreted By similarity.
Post-translational modification	Undergoes proteolysis by a metalloprotease to give rise to a soluble monomeric form By similarity.
Sequence similarities	Belongs to the ephrin family. Contains 1 ephrin RBD (ephrin receptor-binding) domain.

Ontologies

Keywords
Biological process	Angiogenesis
Cellular component	Cell membrane Membrane Secreted
Disease	Tumor suppressor
Domain	Signal
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of MAPK activity Inferred from electronic annotation. Source: Compara angiogenesis Non-traceable author statement PubMed 12615978. Source: RGD aortic valve morphogenesis Inferred from electronic annotation. Source: Compara cell migration Inferred from electronic annotation. Source: Compara endocardial cushion to mesenchymal transition involved in heart valve formation Inferred from electronic annotation. Source: Compara ephrin receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB mitral valve morphogenesis Inferred from electronic annotation. Source: Compara negative regulation of dendritic spine morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of epithelial to mesenchymal transition Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara neuron differentiation Inferred from electronic annotation. Source: Compara notochord formation Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-tyrosine phosphorylation Inferred from electronic annotation. Source: Compara regulation of angiogenesis Inferred from electronic annotation. Source: Compara regulation of axonogenesis Inferred from electronic annotation. Source: Compara regulation of blood vessel endothelial cell migration Inferred from electronic annotation. Source: Compara regulation of cell adhesion mediated by integrin Inferred from electronic annotation. Source: Compara regulation of peptidyl-tyrosine phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB substrate adhesion-dependent cell spreading Inferred from electronic annotation. Source: Compara
Cellular_component	anchored to plasma membrane Inferred from electronic annotation. Source: Compara extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ephrin receptor binding Traceable author statement PubMed 12615978. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 182	165	Ephrin-A1		PRO_0000008357
	Chain	18 – ?		Ephrin-A1, secreted form		PRO_0000389633
	Propeptide	183 – 205	23	Removed in mature form Potential		PRO_0000008358
Regions
	Domain	18 – 151	134	Ephrin RBD
Amino acid modifications
	Lipidation	182	1	GPI-anchor amidated serine Potential
	Glycosylation	26	1	N-linked (GlcNAc...) Potential
	Disulfide bond	51 ↔ 92		By similarity
	Disulfide bond	80 ↔ 140		By similarity
Experimental info
	Sequence conflict	18	1	A → V in BAA07242. Ref.1
	Sequence conflict	68	1	T → S in BAA07242. Ref.1
	Sequence conflict	90	1	W → V in BAA07242. Ref.1
	Sequence conflict	159	1	A → V in BAA07242. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P97553 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: E9ED3F73AE7FC4CA	FASTA	205	23,763
	10 20 30 40 50 60 MEFLWAPLLG LCCSLAAADR HIVFWNSSNP KFREEDYTVH VQLNDYLDII CPHYEDDSVA 70 80 90 100 110 120 DAAMERYTLY MVEHQEYVTC EPQSKDQVRW KCNQPSAKHG PEKLSEKFQR FTPFTLGKEF 130 140 150 160 170 180 KEGHSYYYIS KPIYHQETQC LKLKVTVNGK ITHSPHAHAN PQEKRLQADD PEVQVLHSIG 190 200 HSAAPRLFPL VWAVLLLPLL LLQTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of rat and mouse B61 gene: implications on organogenesis." Takahashi H., Ikeda T. Oncogene 11:879-883(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38056 mRNA. Translation: BAA07242.1. BC070514 mRNA. Translation: AAH70514.1.
IPI	IPI00205523.
RefSeq	NP_446051.2. NM_053599.2.
UniGene	Rn.8427.
3D structure databases
ProteinModelPortal	P97553.
SMR	P97553. Positions 18-149.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000027920.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000027920; ENSRNOP00000027920; ENSRNOG00000020573.
GeneID	94268.
KEGG	rno:94268.
UCSC	RGD:620388. rat.
Organism-specific databases
CTD	1942.
RGD	620388. Efna1.
Phylogenomic databases
eggNOG	NOG296825.
GeneTree	ENSGT00390000015107.
HOGENOM	HOG000234373.
HOVERGEN	HBG051447.
InParanoid	P97553.
KO	K05462.
OMA	PIHHQED.
OrthoDB	EOG4D52ZP.
Gene expression databases
Genevestigator	P97553.
GermOnline	ENSRNOG00000020573. Rattus norvegicus.
Family and domain databases
Gene3D	2.60.40.420. 1 hit.
InterPro	IPR008972. Cupredoxin. IPR001799. Ephrin. IPR019765. Ephrin_CS. [Graphical view]
PANTHER	PTHR11304. PTHR11304. 1 hit.
Pfam	PF00812. Ephrin. 1 hit. [Graphical view]
PRINTS	PR01347. EPHRIN.
ProDom	PD002533. Ephrin. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF49503. Cupredoxin. 1 hit.
PROSITE	PS01299. EPHRIN_RBD_1. 1 hit. PS51551. EPHRIN_RBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	617895.

Entry information

Entry name

EFNA1_RAT

Accession

Primary (citable) accession number: P97553
Secondary accession number(s): Q6NS29

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 30, 2005
Last modified:	April 3, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program