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Protein

Ephrin-A1

Gene

Efna1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAngiogenesis

Enzyme and pathway databases

ReactomeiR-RNO-2682334 EPH-Ephrin signaling
R-RNO-3928663 EPHA-mediated growth cone collapse
R-RNO-3928665 EPH-ephrin mediated repulsion of cells

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin-A1
Alternative name(s):
EPH-related receptor tyrosine kinase ligand 1
Short name:
LERK-1
Immediate early response protein B61
Cleaved into the following chain:
Gene namesi
Name:Efna1
Synonyms:Epgl1, Lerk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi620388 Efna1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000000835718 – 182Ephrin-A1Add BLAST165
ChainiPRO_000038963318 – ?Ephrin-A1, secreted form
PropeptideiPRO_0000008358183 – 205Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi26N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi51 ↔ 92PROSITE-ProRule annotation
Disulfide bondi80 ↔ 140PROSITE-ProRule annotation
Lipidationi182GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

Undergoes proteolysis by a metalloprotease to give rise to a soluble monomeric form.By similarity
N-Glycosylation is required for binding to EPHA2 receptor and inducing its internalization.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP97553

Expressioni

Gene expression databases

BgeeiENSRNOG00000020573
GenevisibleiP97553 RN

Interactioni

Subunit structurei

Monomer. Homodimer. Forms heterodimers with EPHA2. Binds to the receptor tyrosine kinases EPHA2, EPHA3, EPHA4, EPHA5, EPHA6 and EPHA7. Also binds with low affinity to EPHA1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027920

Structurei

3D structure databases

ProteinModelPortaliP97553
SMRiP97553
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 151Ephrin RBDPROSITE-ProRule annotationAdd BLAST134

Sequence similaritiesi

Belongs to the ephrin family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3858 Eukaryota
ENOG4111FMJ LUCA
GeneTreeiENSGT00390000015107
HOGENOMiHOG000234373
HOVERGENiHBG051447
InParanoidiP97553
KOiK05462
OMAiCPHYEDD
OrthoDBiEOG091G0K52
PhylomeDBiP97553

Family and domain databases

CDDicd10425 Ephrin-A_Ectodomain, 1 hit
Gene3Di2.60.40.420, 1 hit
InterProiView protein in InterPro
IPR008972 Cupredoxin
IPR031328 Ephrin
IPR034252 Ephrin-A_Ecto
IPR019765 Ephrin_CS
IPR001799 Ephrin_RBD
PANTHERiPTHR11304 PTHR11304, 1 hit
PfamiView protein in Pfam
PF00812 Ephrin, 1 hit
PRINTSiPR01347 EPHRIN
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002533 Ephrin, 1 hit
SUPFAMiSSF49503 SSF49503, 1 hit
PROSITEiView protein in PROSITE
PS01299 EPHRIN_RBD_1, 1 hit
PS51551 EPHRIN_RBD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97553-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFLWAPLLG LCCSLAAADR HIVFWNSSNP KFREEDYTVH VQLNDYLDII
60 70 80 90 100
CPHYEDDSVA DAAMERYTLY MVEHQEYVTC EPQSKDQVRW KCNQPSAKHG
110 120 130 140 150
PEKLSEKFQR FTPFTLGKEF KEGHSYYYIS KPIYHQETQC LKLKVTVNGK
160 170 180 190 200
ITHSPHAHAN PQEKRLQADD PEVQVLHSIG HSAAPRLFPL VWAVLLLPLL

LLQTQ
Length:205
Mass (Da):23,763
Last modified:August 30, 2005 - v2
Checksum:iE9ED3F73AE7FC4CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18A → V in BAA07242 (PubMed:7675446).Curated1
Sequence conflicti68T → S in BAA07242 (PubMed:7675446).Curated1
Sequence conflicti90W → V in BAA07242 (PubMed:7675446).Curated1
Sequence conflicti159A → V in BAA07242 (PubMed:7675446).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38056 mRNA Translation: BAA07242.1
BC070514 mRNA Translation: AAH70514.1
RefSeqiNP_446051.2, NM_053599.2
UniGeneiRn.8427

Genome annotation databases

EnsembliENSRNOT00000027920; ENSRNOP00000027920; ENSRNOG00000020573
GeneIDi94268
KEGGirno:94268
UCSCiRGD:620388 rat

Similar proteinsi

Entry informationi

Entry nameiEFNA1_RAT
AccessioniPrimary (citable) accession number: P97553
Secondary accession number(s): Q6NS29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 30, 2005
Last modified: March 28, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health