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P97553

- EFNA1_RAT

UniProt

P97553 - EFNA1_RAT

Protein

Ephrin-A1

Gene

Efna1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis By similarity.By similarity

    GO - Molecular functioni

    1. ephrin receptor binding Source: RefGenome

    GO - Biological processi

    1. activation of MAPK activity Source: Ensembl
    2. angiogenesis Source: RGD
    3. aortic valve morphogenesis Source: Ensembl
    4. axon guidance Source: RefGenome
    5. cell migration Source: Ensembl
    6. endocardial cushion to mesenchymal transition involved in heart valve formation Source: Ensembl
    7. ephrin receptor signaling pathway Source: UniProtKB
    8. mitral valve morphogenesis Source: Ensembl
    9. negative regulation of dendritic spine morphogenesis Source: UniProtKB
    10. negative regulation of epithelial to mesenchymal transition Source: Ensembl
    11. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    12. notochord formation Source: Ensembl
    13. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
    14. regulation of angiogenesis Source: Ensembl
    15. regulation of axonogenesis Source: Ensembl
    16. regulation of blood vessel endothelial cell migration Source: Ensembl
    17. regulation of cell adhesion mediated by integrin Source: Ensembl
    18. regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
    19. substrate adhesion-dependent cell spreading Source: Ensembl

    Keywords - Biological processi

    Angiogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ephrin-A1
    Alternative name(s):
    EPH-related receptor tyrosine kinase ligand 1
    Short name:
    LERK-1
    Immediate early response protein B61
    Cleaved into the following chain:
    Gene namesi
    Name:Efna1
    Synonyms:Epgl1, Lerk1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi620388. Efna1.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

    GO - Cellular componenti

    1. anchored component of plasma membrane Source: RefGenome
    2. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    Pathology & Biotechi

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 182165Ephrin-A1PRO_0000008357Add
    BLAST
    Chaini18 – ?Ephrin-A1, secreted formPRO_0000389633
    Propeptidei183 – 20523Removed in mature formSequence AnalysisPRO_0000008358Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi26 – 261N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi51 ↔ 92PROSITE-ProRule annotation
    Disulfide bondi80 ↔ 140PROSITE-ProRule annotation
    Lipidationi182 – 1821GPI-anchor amidated serineSequence Analysis

    Post-translational modificationi

    Undergoes proteolysis by a metalloprotease to give rise to a soluble monomeric form.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriP97553.

    Interactioni

    Subunit structurei

    Monomer. Homodimer. Forms heterodimers with EPHA2. Binds to the receptor tyrosine kinases EPHA2, EPHA3, EPHA4, EPHA5, EPHA6 and EPHA7. Also binds with low affinity to EPHA1 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000027920.

    Structurei

    3D structure databases

    ProteinModelPortaliP97553.
    SMRiP97553. Positions 18-149.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 151134Ephrin RBDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ephrin family.PROSITE-ProRule annotation
    Contains 1 ephrin RBD (ephrin receptor-binding) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG296825.
    GeneTreeiENSGT00390000015107.
    HOGENOMiHOG000234373.
    HOVERGENiHBG051447.
    InParanoidiP97553.
    KOiK05462.
    OMAiPIHHQED.
    OrthoDBiEOG70W3FD.
    PhylomeDBiP97553.

    Family and domain databases

    Gene3Di2.60.40.420. 1 hit.
    InterProiIPR008972. Cupredoxin.
    IPR001799. Ephrin.
    IPR019765. Ephrin_CS.
    [Graphical view]
    PANTHERiPTHR11304. PTHR11304. 1 hit.
    PfamiPF00812. Ephrin. 1 hit.
    [Graphical view]
    PRINTSiPR01347. EPHRIN.
    ProDomiPD002533. Ephrin. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF49503. SSF49503. 1 hit.
    PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
    PS51551. EPHRIN_RBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97553-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFLWAPLLG LCCSLAAADR HIVFWNSSNP KFREEDYTVH VQLNDYLDII    50
    CPHYEDDSVA DAAMERYTLY MVEHQEYVTC EPQSKDQVRW KCNQPSAKHG 100
    PEKLSEKFQR FTPFTLGKEF KEGHSYYYIS KPIYHQETQC LKLKVTVNGK 150
    ITHSPHAHAN PQEKRLQADD PEVQVLHSIG HSAAPRLFPL VWAVLLLPLL 200
    LLQTQ 205
    Length:205
    Mass (Da):23,763
    Last modified:August 30, 2005 - v2
    Checksum:iE9ED3F73AE7FC4CA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti18 – 181A → V in BAA07242. (PubMed:7675446)Curated
    Sequence conflicti68 – 681T → S in BAA07242. (PubMed:7675446)Curated
    Sequence conflicti90 – 901W → V in BAA07242. (PubMed:7675446)Curated
    Sequence conflicti159 – 1591A → V in BAA07242. (PubMed:7675446)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38056 mRNA. Translation: BAA07242.1.
    BC070514 mRNA. Translation: AAH70514.1.
    RefSeqiNP_446051.2. NM_053599.2.
    UniGeneiRn.8427.

    Genome annotation databases

    EnsembliENSRNOT00000027920; ENSRNOP00000027920; ENSRNOG00000020573.
    GeneIDi94268.
    KEGGirno:94268.
    UCSCiRGD:620388. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38056 mRNA. Translation: BAA07242.1 .
    BC070514 mRNA. Translation: AAH70514.1 .
    RefSeqi NP_446051.2. NM_053599.2.
    UniGenei Rn.8427.

    3D structure databases

    ProteinModelPortali P97553.
    SMRi P97553. Positions 18-149.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000027920.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027920 ; ENSRNOP00000027920 ; ENSRNOG00000020573 .
    GeneIDi 94268.
    KEGGi rno:94268.
    UCSCi RGD:620388. rat.

    Organism-specific databases

    CTDi 1942.
    RGDi 620388. Efna1.

    Phylogenomic databases

    eggNOGi NOG296825.
    GeneTreei ENSGT00390000015107.
    HOGENOMi HOG000234373.
    HOVERGENi HBG051447.
    InParanoidi P97553.
    KOi K05462.
    OMAi PIHHQED.
    OrthoDBi EOG70W3FD.
    PhylomeDBi P97553.

    Miscellaneous databases

    NextBioi 617895.
    PROi P97553.

    Gene expression databases

    Genevestigatori P97553.

    Family and domain databases

    Gene3Di 2.60.40.420. 1 hit.
    InterProi IPR008972. Cupredoxin.
    IPR001799. Ephrin.
    IPR019765. Ephrin_CS.
    [Graphical view ]
    PANTHERi PTHR11304. PTHR11304. 1 hit.
    Pfami PF00812. Ephrin. 1 hit.
    [Graphical view ]
    PRINTSi PR01347. EPHRIN.
    ProDomi PD002533. Ephrin. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF49503. SSF49503. 1 hit.
    PROSITEi PS01299. EPHRIN_RBD_1. 1 hit.
    PS51551. EPHRIN_RBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of rat and mouse B61 gene: implications on organogenesis."
      Takahashi H., Ikeda T.
      Oncogene 11:879-883(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.

    Entry informationi

    Entry nameiEFNA1_RAT
    AccessioniPrimary (citable) accession number: P97553
    Secondary accession number(s): Q6NS29
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3