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Protein

Phospholipase A1 member A

Gene

Pla1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production.2 Publications

Kineticsi

    1. Vmax=2.6 µmol/min/mg enzyme with phosphatidylcholine as substrate1 Publication
    2. Vmax=0.1 µmol/min/mg enzyme with phosphatidylinositol as substrate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei166 – 1661NucleophileBy similarity
    Active sitei190 – 1901Charge relay systemPROSITE-ProRule annotation
    Active sitei260 – 2601Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Protein family/group databases

    ESTHERiratno-P97535. Phospholipase.

    Chemistry

    SwissLipidsiSLP:000000649.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipase A1 member A (EC:3.1.1.-)
    Alternative name(s):
    Phosphatidylserine-specific phospholipase A1
    Short name:
    PS-PLA1
    Gene namesi
    Name:Pla1a
    Synonyms:Pspla1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 11

    Organism-specific databases

    RGDi621261. Pla1a.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Chemistry

    ChEMBLiCHEMBL2307.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24241 PublicationAdd
    BLAST
    Chaini25 – 456432Phospholipase A1 member APRO_0000273333Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi245 ↔ 258By similarity
    Disulfide bondi282 ↔ 293By similarity
    Disulfide bondi296 ↔ 304By similarity
    Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP97535.
    PRIDEiP97535.

    Expressioni

    Gene expression databases

    BgeeiENSRNOG00000027252.
    GenevisibleiP97535. RN.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000035788.

    Structurei

    3D structure databases

    ProteinModelPortaliP97535.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily. Lipase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IJ4N. Eukaryota.
    ENOG4110U23. LUCA.
    GeneTreeiENSGT00760000119069.
    HOGENOMiHOG000059578.
    HOVERGENiHBG082148.
    InParanoidiP97535.
    KOiK13618.
    OMAiAPYCVHH.
    OrthoDBiEOG091G0680.
    PhylomeDBiP97535.
    TreeFamiTF324997.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR016272. Lipase_LIPH.
    IPR013818. Lipase_N.
    IPR000734. TAG_lipase.
    [Graphical view]
    PANTHERiPTHR11610. PTHR11610. 1 hit.
    PfamiPF00151. Lipase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
    PRINTSiPR00821. TAGLIPASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00120. LIPASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97535-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MCPGLWGTCF WLWGSLLWLS IGRSGNVPPT TQPKCTDFQS ANLLRGTNLK
    60 70 80 90 100
    VQFLLFTPSD PGCGQLVEED SDIRNSEFNA SLGTKLIIHG FRALGTKPSW
    110 120 130 140 150
    INKFIRALLR AADANVIAVD WVYGSTGMYF SAVENVVKLS LEISRFLSKL
    160 170 180 190 200
    LELGVSESSI HIIGVSLGAH VGGMVGHFYK GQLGRITGLD PAGPEYTRAS
    210 220 230 240 250
    LEERLDSGDA LFVEAIHTDT DNLGIRIPVG HVDYFVNGGQ DQPGCPAFIH
    260 270 280 290 300
    AGYSYLICDH MRAVHLYISA LENTCPLMAF PCASYKAFLA GDCLDCFNPF
    310 320 330 340 350
    LLSCPRIGLV ERGGVKIEPL PKEVRVYLQT TSSAPYCVHH SLVEFNLKEK
    360 370 380 390 400
    RKKDTSIEVT FLGNNVTSSV KITIPKDHLE GRGIIAHQNP HCQINQVKLK
    410 420 430 440 450
    FHISSRVWRK DRTPIVGTFC TAPLPVNDSK KTVCIPEPVR LQVSMAVLRD

    LKMACV
    Length:456
    Mass (Da):50,202
    Last modified:May 1, 1997 - v1
    Checksum:i0FE091FE165C2879
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti30 – 301T → P AA sequence (PubMed:8999922).Curated
    Sequence conflicti293 – 2931C → S AA sequence (PubMed:8999922).Curated
    Sequence conflicti296 – 2961C → S AA sequence (PubMed:8999922).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D88666 mRNA. Translation: BAA13672.1.
    BC078727 mRNA. Translation: AAH78727.1.
    RefSeqiNP_620237.1. NM_138882.1.
    UniGeneiRn.10696.

    Genome annotation databases

    EnsembliENSRNOT00000077727; ENSRNOP00000071860; ENSRNOG00000057153.
    GeneIDi85311.
    KEGGirno:85311.
    UCSCiRGD:621261. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D88666 mRNA. Translation: BAA13672.1.
    BC078727 mRNA. Translation: AAH78727.1.
    RefSeqiNP_620237.1. NM_138882.1.
    UniGeneiRn.10696.

    3D structure databases

    ProteinModelPortaliP97535.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000035788.

    Chemistry

    ChEMBLiCHEMBL2307.
    SwissLipidsiSLP:000000649.

    Protein family/group databases

    ESTHERiratno-P97535. Phospholipase.

    Proteomic databases

    PaxDbiP97535.
    PRIDEiP97535.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSRNOT00000077727; ENSRNOP00000071860; ENSRNOG00000057153.
    GeneIDi85311.
    KEGGirno:85311.
    UCSCiRGD:621261. rat.

    Organism-specific databases

    CTDi51365.
    RGDi621261. Pla1a.

    Phylogenomic databases

    eggNOGiENOG410IJ4N. Eukaryota.
    ENOG4110U23. LUCA.
    GeneTreeiENSGT00760000119069.
    HOGENOMiHOG000059578.
    HOVERGENiHBG082148.
    InParanoidiP97535.
    KOiK13618.
    OMAiAPYCVHH.
    OrthoDBiEOG091G0680.
    PhylomeDBiP97535.
    TreeFamiTF324997.

    Miscellaneous databases

    PROiP97535.

    Gene expression databases

    BgeeiENSRNOG00000027252.
    GenevisibleiP97535. RN.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR016272. Lipase_LIPH.
    IPR013818. Lipase_N.
    IPR000734. TAG_lipase.
    [Graphical view]
    PANTHERiPTHR11610. PTHR11610. 1 hit.
    PfamiPF00151. Lipase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
    PRINTSiPR00821. TAGLIPASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00120. LIPASE_SER. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPLA1A_RAT
    AccessioniPrimary (citable) accession number: P97535
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: May 1, 1997
    Last modified: September 7, 2016
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.