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Protein

Phospholipase A1 member A

Gene

Pla1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production.2 Publications

Kineticsi

    1. Vmax=2.6 µmol/min/mg enzyme with phosphatidylcholine as substrate1 Publication
    2. Vmax=0.1 µmol/min/mg enzyme with phosphatidylinositol as substrate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei166NucleophileBy similarity1
    Active sitei190Charge relay systemPROSITE-ProRule annotation1
    Active sitei260Charge relay systemPROSITE-ProRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    Biological processLipid degradation, Lipid metabolism

    Enzyme and pathway databases

    ReactomeiR-RNO-1482801 Acyl chain remodelling of PS

    Protein family/group databases

    ESTHERiratno-P97535 Phospholipase

    Chemistry databases

    SwissLipidsiSLP:000000649

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipase A1 member A (EC:3.1.1.-)
    Alternative name(s):
    Phosphatidylserine-specific phospholipase A1
    Short name:
    PS-PLA1
    Gene namesi
    Name:Pla1a
    Synonyms:Pspla1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 11

    Organism-specific databases

    RGDi621261 Pla1a

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL2307

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 241 PublicationAdd BLAST24
    ChainiPRO_000027333325 – 456Phospholipase A1 member AAdd BLAST432

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi245 ↔ 258By similarity
    Disulfide bondi282 ↔ 293By similarity
    Disulfide bondi296 ↔ 304By similarity
    Glycosylationi365N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP97535
    PRIDEiP97535

    Expressioni

    Gene expression databases

    BgeeiENSRNOG00000057153
    GenevisibleiP97535 RN

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000035788

    Structurei

    3D structure databases

    ProteinModelPortaliP97535
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily. Lipase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IJ4N Eukaryota
    ENOG4110U23 LUCA
    GeneTreeiENSGT00760000119069
    HOGENOMiHOG000059578
    HOVERGENiHBG082148
    InParanoidiP97535
    KOiK13618
    OMAiAPYCVHH
    OrthoDBiEOG091G0680
    PhylomeDBiP97535
    TreeFamiTF324997

    Family and domain databases

    CDDicd00707 Pancreat_lipase_like, 1 hit
    Gene3Di3.40.50.18201 hit
    InterProiView protein in InterPro
    IPR029058 AB_hydrolase
    IPR013818 Lipase/vitellogenin
    IPR016272 Lipase_LIPH
    IPR033906 Lipase_N
    IPR000734 TAG_lipase
    PANTHERiPTHR11610 PTHR11610, 1 hit
    PfamiView protein in Pfam
    PF00151 Lipase, 1 hit
    PIRSFiPIRSF000865 Lipoprotein_lipase_LIPH, 1 hit
    PRINTSiPR00821 TAGLIPASE
    SUPFAMiSSF53474 SSF53474, 1 hit
    PROSITEiView protein in PROSITE
    PS00120 LIPASE_SER, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97535-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MCPGLWGTCF WLWGSLLWLS IGRSGNVPPT TQPKCTDFQS ANLLRGTNLK
    60 70 80 90 100
    VQFLLFTPSD PGCGQLVEED SDIRNSEFNA SLGTKLIIHG FRALGTKPSW
    110 120 130 140 150
    INKFIRALLR AADANVIAVD WVYGSTGMYF SAVENVVKLS LEISRFLSKL
    160 170 180 190 200
    LELGVSESSI HIIGVSLGAH VGGMVGHFYK GQLGRITGLD PAGPEYTRAS
    210 220 230 240 250
    LEERLDSGDA LFVEAIHTDT DNLGIRIPVG HVDYFVNGGQ DQPGCPAFIH
    260 270 280 290 300
    AGYSYLICDH MRAVHLYISA LENTCPLMAF PCASYKAFLA GDCLDCFNPF
    310 320 330 340 350
    LLSCPRIGLV ERGGVKIEPL PKEVRVYLQT TSSAPYCVHH SLVEFNLKEK
    360 370 380 390 400
    RKKDTSIEVT FLGNNVTSSV KITIPKDHLE GRGIIAHQNP HCQINQVKLK
    410 420 430 440 450
    FHISSRVWRK DRTPIVGTFC TAPLPVNDSK KTVCIPEPVR LQVSMAVLRD

    LKMACV
    Length:456
    Mass (Da):50,202
    Last modified:May 1, 1997 - v1
    Checksum:i0FE091FE165C2879
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti30T → P AA sequence (PubMed:8999922).Curated1
    Sequence conflicti293C → S AA sequence (PubMed:8999922).Curated1
    Sequence conflicti296C → S AA sequence (PubMed:8999922).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D88666 mRNA Translation: BAA13672.1
    BC078727 mRNA Translation: AAH78727.1
    RefSeqiNP_620237.1, NM_138882.1
    UniGeneiRn.10696

    Genome annotation databases

    EnsembliENSRNOT00000077727; ENSRNOP00000071860; ENSRNOG00000057153
    GeneIDi85311
    KEGGirno:85311
    UCSCiRGD:621261 rat

    Similar proteinsi

    Entry informationi

    Entry nameiPLA1A_RAT
    AccessioniPrimary (citable) accession number: P97535
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: May 1, 1997
    Last modified: November 22, 2017
    This is version 113 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome