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P97531

- CIP4_RAT

UniProt

P97531 - CIP4_RAT

Protein

Cdc42-interacting protein 4

Gene

Trip10

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 3 (28 Nov 2006)
      Previous versions | rss
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    Functioni

    Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Also acts as a link between CDC42 signaling and regulation of the actin cytoskeleton. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also enhances actin polymerization in the vicinity of membrane tubules by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization and dynamin may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling. May be required for the lysosomal retention of FASLG/FASL By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei166 – 1661Mediates end-to-end attachment of dimersBy similarity

    GO - Molecular functioni

    1. lipid binding Source: UniProtKB-KW

    GO - Biological processi

    1. actin cytoskeleton organization Source: InterPro
    2. endocytosis Source: UniProtKB-KW
    3. hyperosmotic salinity response Source: RGD

    Keywords - Biological processi

    Endocytosis

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cdc42-interacting protein 4
    Alternative name(s):
    Salt tolerant protein
    Thyroid receptor-interacting protein 10
    Short name:
    TR-interacting protein 10
    Short name:
    TRIP-10
    Gene namesi
    Name:Trip10
    Synonyms:Cip4, Stp
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621145. Trip10.

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity. Cytoplasmcell cortex By similarity. Lysosome By similarity. Golgi apparatus By similarity. Cell membrane By similarity. Cell projectionphagocytic cup By similarity
    Note: Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ By similarity.By similarity

    GO - Cellular componenti

    1. cell cortex Source: UniProtKB-SubCell
    2. cell projection Source: UniProtKB-KW
    3. cytoskeleton Source: UniProtKB-SubCell
    4. Golgi apparatus Source: UniProtKB-SubCell
    5. lysosome Source: UniProtKB-SubCell
    6. phagocytic cup Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 547547Cdc42-interacting protein 4PRO_0000261441Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei296 – 2961PhosphoserineBy similarity
    Modified residuei299 – 2991PhosphoserineBy similarity
    Modified residuei426 – 4261PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP97531.

    PTM databases

    PhosphoSiteiP97531.

    Expressioni

    Tissue specificityi

    Expressed in adrenal gland, aorta, brain, heart, kidney, liver, skeletal muscle and spleen.1 Publication

    Gene expression databases

    GenevestigatoriP97531.

    Interactioni

    Subunit structurei

    Homodimerizes, the dimers can polymerize end-to-end to form filamentous structures. Interacts specifically with GTP-bound CDC42 and RHOQ. Interacts with AKAP9, ARHGAP17, DAAM1, DIAPH1, DIAPH2, DNM1, DNM2, FASLG/FASL, GAPVD1, LYN, microtubules, SRC, WAS/WASP and WASL/N-WASP. Interacts with the ligand binding domain of the thyroid receptor (TR) in the presence of thyroid hormone. May interact with CTNNB1 and HD/HTT By similarity. Interacts with PDE6G.By similarity1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP97531.
    SMRiP97531. Positions 10-288, 490-545.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 6565FCHPROSITE-ProRule annotationAdd
    BLAST
    Repeati349 – 42577REMAdd
    BLAST
    Domaini486 – 54762SH3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 288288F-BAR domainBy similarityAdd
    BLAST
    Regioni1 – 117117Required for translocation to the plasma membrane in response to insulin, podosome formation and interaction with AKAP9 and microtubulesBy similarityAdd
    BLAST
    Regioni293 – 547255Interaction with PDE6GAdd
    BLAST
    Regioni293 – 483191Interaction with CDC42By similarityAdd
    BLAST
    Regioni415 – 547133Required for interaction with FASLG and localization to lysosomesBy similarityAdd
    BLAST
    Regioni431 – 48757Interaction with DNM2 and WASLBy similarityAdd
    BLAST
    Regioni476 – 54772Interaction with DNM1 and WASLBy similarityAdd
    BLAST
    Regioni484 – 54764Required for podosome formationBy similarityAdd
    BLAST
    Regioni490 – 54758Interaction with WASBy similarityAdd
    BLAST
    Regioni492 – 54756Interaction with ARHGAP17, DAAM1, DIAPH1 and DIAPH2By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili67 – 259193By similarityAdd
    BLAST
    Coiled coili332 – 42594By similarityAdd
    BLAST

    Domaini

    The F-BAR domain binds the phospholipid membrane with its concave surface. The end-to-end polymerization of dimers of these domains provides a curved surface that fits best membranes with around 600 A diameter, and may drive tubulation By similarity.By similarity

    Sequence similaritiesi

    Belongs to the FNBP1 family.Curated
    Contains 1 FCH domain.PROSITE-ProRule annotation
    Contains 1 REM (Hr1) repeat.Curated
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, SH3 domain

    Phylogenomic databases

    HOVERGENiHBG002489.
    KOiK07196.
    PhylomeDBiP97531.

    Family and domain databases

    InterProiIPR028498. CIP4.
    IPR001060. FCH_dom.
    IPR001452. SH3_domain.
    [Graphical view]
    PANTHERiPTHR12602:SF7. PTHR12602:SF7. 1 hit.
    PfamiPF00611. FCH. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    SMARTiSM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 2 hits.
    PROSITEiPS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P97531-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDWGTELWDQ FEVLERHTQW GLDLLDKYVK FVKERVEVEQ SYAKQLRSLV    50
    KKYLPKRPAK DDPEIKFSQQ QSFVQLLQEV NDFAGQRELV AESLGIRVCL 100
    ELAKYSQEMK QERKMHFQEG RRAQQQLENG FKQLENSKRK FERDCREAEK 150
    AAHTAERLDQ DINATKADVE KAKQQAHLRN HMAEESKNEY AAQLQRFNRD 200
    QAHFYFSQMP QIFDKLQDMD ERRATRLGAG YGLLSEAELQ VVPIIGKCLE 250
    GMKVAAESVD AKNDSKVLIE LHKSGFARPG DLEFEDFSQV MNRVPSDSSL 300
    GTPDGRPELR AASSRSRAKR WPFGKKNKTV VTEDFSHLPP EQQRKRLQQQ 350
    LEERNRELQK EEDQREALKK MKDVYEKTPQ MGDPASLEPR IAETLGNIER 400
    LKLEVQKYEA WLAEAESRVL SNRGDSLSRH TRPPDPPTTA PPDSSSSSNN 450
    SGSQDNKESS EEPPSEEGQD TPIYTEFDED FEEPASPIGQ CVAIYHFEGS 500
    SEGTVSMSEG EDLSLMEEDK GDGWTRVRRK QGGEGYVPTS YLRVTLN 547
    Length:547
    Mass (Da):62,799
    Last modified:November 28, 2006 - v3
    Checksum:iEE995A691E661DE5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti402 – 4032KL → NV in BAA22191. (PubMed:8954095)Curated
    Sequence conflicti417 – 4171S → T in BAA22191. (PubMed:8954095)Curated
    Sequence conflicti457 – 4571K → M in BAA22191. (PubMed:8954095)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006914 mRNA. Translation: BAA22191.1.
    BC061840 mRNA. Translation: AAH61840.1.
    PIRiJC5261.
    RefSeqiNP_446372.1. NM_053920.1.
    UniGeneiRn.10814.

    Genome annotation databases

    GeneIDi116717.
    KEGGirno:116717.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006914 mRNA. Translation: BAA22191.1 .
    BC061840 mRNA. Translation: AAH61840.1 .
    PIRi JC5261.
    RefSeqi NP_446372.1. NM_053920.1.
    UniGenei Rn.10814.

    3D structure databases

    ProteinModelPortali P97531.
    SMRi P97531. Positions 10-288, 490-545.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P97531.

    Proteomic databases

    PRIDEi P97531.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 116717.
    KEGGi rno:116717.

    Organism-specific databases

    CTDi 9322.
    RGDi 621145. Trip10.

    Phylogenomic databases

    HOVERGENi HBG002489.
    KOi K07196.
    PhylomeDBi P97531.

    Miscellaneous databases

    NextBioi 619616.
    PROi P97531.

    Gene expression databases

    Genevestigatori P97531.

    Family and domain databases

    InterProi IPR028498. CIP4.
    IPR001060. FCH_dom.
    IPR001452. SH3_domain.
    [Graphical view ]
    PANTHERi PTHR12602:SF7. PTHR12602:SF7. 1 hit.
    Pfami PF00611. FCH. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    SMARTi SM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 2 hits.
    PROSITEi PS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a novel rat salt-tolerant protein by functional complementation in yeast."
      Tsuji E., Tsuji Y., Misumi Y., Fujita A., Sasaguri M., Ideishi M., Arakawa K.
      Biochem. Biophys. Res. Commun. 229:134-138(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: Wistar.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    3. Lubec G., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 391-402, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.
    4. "A proline-rich domain in the gamma subunit of phosphodiesterase 6 mediates interaction with SH3-containing proteins."
      Morin F., Vannier B., Houdart F., Regnacq M., Berges T., Voisin P.
      Mol. Vis. 9:449-459(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PDE6G.

    Entry informationi

    Entry nameiCIP4_RAT
    AccessioniPrimary (citable) accession number: P97531
    Secondary accession number(s): Q6P744
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: November 28, 2006
    Last modified: October 1, 2014
    This is version 81 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3