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P97526

- NF1_RAT

UniProt

P97526 - NF1_RAT

Protein

Neurofibromin

Gene

Nf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity By similarity.By similarity

    GO - Molecular functioni

    1. microtubule binding Source: MGI
    2. phosphatidylcholine binding Source: UniProtKB
    3. phosphatidylethanolamine binding Source: UniProtKB
    4. protein binding Source: RGD
    5. Ras GTPase activator activity Source: UniProtKB
    6. syndecan binding Source: RGD

    GO - Biological processi

    1. actin cytoskeleton organization Source: HGNC
    2. adrenal gland development Source: HGNC
    3. artery morphogenesis Source: HGNC
    4. brain development Source: HGNC
    5. camera-type eye morphogenesis Source: HGNC
    6. cell communication Source: HGNC
    7. cerebral cortex development Source: HGNC
    8. collagen fibril organization Source: HGNC
    9. extracellular matrix organization Source: HGNC
    10. forebrain astrocyte development Source: HGNC
    11. forebrain morphogenesis Source: HGNC
    12. heart development Source: HGNC
    13. liver development Source: HGNC
    14. MAPK cascade Source: HGNC
    15. metanephros development Source: HGNC
    16. myelination in peripheral nervous system Source: HGNC
    17. negative regulation of fibroblast proliferation Source: UniProtKB
    18. negative regulation of MAPK cascade Source: HGNC
    19. negative regulation of MAP kinase activity Source: HGNC
    20. negative regulation of neuroblast proliferation Source: HGNC
    21. negative regulation of oligodendrocyte differentiation Source: HGNC
    22. negative regulation of protein kinase activity Source: HGNC
    23. negative regulation of Ras protein signal transduction Source: RefGenome
    24. negative regulation of transcription factor import into nucleus Source: HGNC
    25. osteoblast differentiation Source: HGNC
    26. peripheral nervous system development Source: HGNC
    27. phosphatidylinositol 3-kinase signaling Source: HGNC
    28. pigmentation Source: HGNC
    29. positive regulation of adenylate cyclase activity Source: HGNC
    30. positive regulation of apoptotic process Source: HGNC
    31. positive regulation of neuron apoptotic process Source: HGNC
    32. positive regulation of Ras GTPase activity Source: UniProtKB
    33. Ras protein signal transduction Source: HGNC
    34. regulation of angiogenesis Source: HGNC
    35. regulation of bone resorption Source: HGNC
    36. regulation of cell-matrix adhesion Source: HGNC
    37. regulation of glial cell differentiation Source: HGNC
    38. regulation of neuron differentiation Source: RGD
    39. regulation of Ras GTPase activity Source: HGNC
    40. response to hypoxia Source: HGNC
    41. Schwann cell development Source: HGNC
    42. smooth muscle tissue development Source: HGNC
    43. spinal cord development Source: HGNC
    44. sympathetic nervous system development Source: HGNC
    45. visual learning Source: HGNC
    46. wound healing Source: HGNC

    Keywords - Molecular functioni

    GTPase activation

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neurofibromin
    Alternative name(s):
    Neurofibromatosis-related protein NF-1
    Gene namesi
    Name:Nf1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3168. Nf1.

    Subcellular locationi

    Nucleus By similarity. Nucleusnucleolus By similarity

    GO - Cellular componenti

    1. axon Source: HGNC
    2. cytoplasm Source: HGNC
    3. dendrite Source: HGNC
    4. intrinsic component of the cytoplasmic side of the plasma membrane Source: RefGenome
    5. membrane Source: RGD
    6. neuron projection Source: RGD
    7. nucleolus Source: UniProtKB-SubCell
    8. nucleus Source: HGNC
    9. postsynaptic density Source: RGD
    10. protein complex Source: RGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 28202819NeurofibrominPRO_0000056667Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei866 – 8661PhosphoserineBy similarity
    Modified residuei878 – 8781PhosphoserineBy similarity
    Modified residuei2169 – 21691PhosphoserineBy similarity
    Modified residuei2496 – 24961PhosphoserineBy similarity
    Modified residuei2502 – 25021PhosphoserineBy similarity
    Modified residuei2524 – 25241PhosphoserineBy similarity
    Modified residuei2798 – 27981PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP97526.
    PRIDEiP97526.

    PTM databases

    PhosphoSiteiP97526.

    Expressioni

    Gene expression databases

    GenevestigatoriP97526.

    Interactioni

    Protein-protein interaction databases

    BioGridi246735. 2 interactions.
    IntActiP97526. 4 interactions.
    MINTiMINT-198504.
    STRINGi10116.ENSRNOP00000049106.

    Structurei

    3D structure databases

    ProteinModelPortaliP97526.
    SMRiP97526. Positions 1208-1531, 1562-1818.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1237 – 1432196Ras-GAPPROSITE-ProRule annotationAdd
    BLAST
    Domaini1561 – 1719159CRAL-TRIOPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1561 – 1818258Lipid bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi2536 – 255217Bipartite nuclear localization signalBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1354 – 13574Poly-Ser

    Domaini

    Binds phospholipids via a region that includes the CRAL-TRIO domain. Binds primarily glycerophospholipids with monounsaturated C18:1 and/or C16:1 fatty acid moieties and a phosphatidylethanolamine or phosphatidylcholine headgroup. Has lesser affinity for lipids containing phosphatidylserine and phosphatidylinositol By similarity.By similarity

    Sequence similaritiesi

    Contains 1 CRAL-TRIO domain.PROSITE-ProRule annotation
    Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5261.
    HOGENOMiHOG000047020.
    HOVERGENiHBG006486.
    KOiK08052.
    PhylomeDBiP97526.

    Family and domain databases

    Gene3Di1.10.506.10. 1 hit.
    1.25.10.10. 7 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR001251. CRAL-TRIO_dom.
    IPR028553. Neurofibromin.
    IPR001936. RasGAP.
    IPR023152. RasGAP_CS.
    IPR008936. Rho_GTPase_activation_prot.
    [Graphical view]
    PANTHERiPTHR10194:SF60. PTHR10194:SF60. 1 hit.
    PfamiPF13716. CRAL_TRIO_2. 1 hit.
    PF00616. RasGAP. 1 hit.
    [Graphical view]
    SMARTiSM00323. RasGAP. 1 hit.
    SM00516. SEC14. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    SSF48371. SSF48371. 8 hits.
    PROSITEiPS50191. CRAL_TRIO. 1 hit.
    PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
    PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97526-1 [UniParc]FASTAAdd to Basket

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    MAAHRPVEWV QAVVSRFDEQ LPIKTGQQNT HTKVSTEHNK ECLINISKYK     50
    FSLVISGLTT ILKNVNNMRI FGEAAEKNLY LSQLIILDTL EKCLAGQPKD 100
    TMRLDETMLV KQLLPEICHF LHTCREGNQH AAELRNSASG VLFSLSCNNF 150
    NAVFSRISTR LQELTVCSED NVDVHDIELL QYINVDCAKL KRLLKETAFK 200
    FKALKKVAQL AVINSLEKAF WNWVENYPDE FTKLYQIPQT DMAECAGKLF 250
    DLVDGFAEST KRKAAVWPLQ IILLILCPEI IQDISRDVVD ENNTNKKLFL 300
    DSLRKALAGH GGSRQLTESA AIACVKLCKA STYINWEDNS VIFLLVQSMV 350
    VDLKNLLFNP SKPFSRGSQP ADVDLMIDCL VSCFRISPHN NQHFKICLAQ 400
    NSPSTFHYVL VNSLHRIITN SAWDWWPKID AVYCHSVELR NMFGETLHKA 450
    VQGCGAHPAL RMAPSLTFKE KVTSLKFKEK PTDLEARSYK YLLLSMVKLI 500
    HADPKLLLCN PRKQGPETQG STAELITGLV QLVPQSHMPE VAQEAMEALL 550
    VLHQLDSIDL WNPDAPVETF WEISSQMLFY ICKKLTSHQM LSSTEILKWL 600
    REILICRNKF LLKNKQADRS SCHSLYLYGV GCDLPASGNV TQMSVDHEES 650
    LRTCAPGASL RKGRGNSSMD STAGCSGTPP ICRQAQTKLE VALYMFLWSP 700
    DTEVVLVAMS CFRHLCEEAD IRCGVDEVSV HNFLPNYNTF MEFASVSNML 750
    STGRAALQKR VMALLRRIEH PTAGNTEAWE DTHAKWEQAT KLILNYPKAK 800
    MEDGQAAESL HKTIVKRRMS HVSGGGSIDL SDTDSLQEWI NMTGFLCALG 850
    GVCLQQRSSS GLATYSPPMG PVSERKGSMI SVMSSEGNVD SPVSRFMDRL 900
    LSLMVCNHEK VGLQIRTNVK DLVGLELSPA LYPMLFNKLK SAISKFFDSQ 950
    GQVLLTDSNT QFVEQTIAIM KNLLDNHTEG SSEHLGQASI ETMMLNLVRY 1000
    VRVLGNMVHA IQIKTKLCQL VEVMMARRDD LSFCQEMKFR NKMVEYLTDW 1050
    VMGTSNQAAD DDVKCLTRDL DQASMEAVVS LLAGLPLQPE EGDGVELMEA 1100
    KSQLFLKYFT LFMNLLNDCS EVEDENAQTG GRKRGMSRRL ASLRHCTVLA 1150
    MSNLLNANVD SGLMHSIGLG YHKDLQTRAT FMEVLTKILQ QGTEFDTLAE 1200
    TVLADRFERL VELVTMMGDQ GELPIAMALA NVVPCSQWDE LARVLVTLFD 1250
    SRHLLYQLLW NMFSKEVELA DSMQTLFRGN SLASKIMTFC FKVYGATYLQ 1300
    KLLDPLLRII ITSSDWQHVS FEVDPTRLEP SESLEENQRN LLQMTEKFFH 1350
    AIISSSSEFP SQLRSVCHCL YQVVSQRFPQ NSIGAVGSAM FLRFINPAIV 1400
    SPYEAGILDK KPPPRIERGL KLMSKVLQSI ANHVLFTKEE HMRPFNDFVK 1450
    SNFDLARRFF LDIASDCPTS DAVNHSLSFI SDGNVLALHR LLWNNQEKIG 1500
    QYLSSNRDHK AVGRRPFDKM ATLLAYLGPP EHKPVADTHW SSLNLTSSKF 1550
    EEFMTRHQVH EKEEFKALKT LSIFYQAGTS KAGNPIFYYV ARRFKTGQIN 1600
    GDLLIYHVLL TLKPYYAKPY EIVVDLTHTG PSNRFKTDFL SKWFVVFPGF 1650
    AYDNVSAVYI YNCNSWVREY TKYHERLLTG LKGSKRLIFI DCPGKLAEHI 1700
    EHEQQKLPAA TLALEEDLKV FHNALKLAHK DTKVSIKVGS TAVQVTSAER 1750
    TKVLGQSVFL NDIYYASEIE EICLVDENQF TLTIANQGTP LTFMHQECEA 1800
    IVQSIIHIRT RWELSQPDSI PQHTKIRPKD VPGTLLNIAL LNLGSSDPSL 1850
    RSAAYNLLCA LTCTFNLKIE GQLLETSGLC IPANNTLFIV SISKTLAANE 1900
    PHLTLEFLEE CISGFSKSSI ELKHLCLEYM TPWLSNLVRF CKHNDDAKRQ 1950
    RVTAILDKLI TMTINEKQMY PSIQAKIWGS LGQITDLLDV VLDSFIKTSA 2000
    TGGLGSIKAE VMADTAVALA SGNVKLVSSK VIGRMCKIID KTCLSPTPTL 2050
    EQHLMWDDIA ILARYMLMLS FNNSLDVAAH LPYLFHVVTF LVATGPLSLR 2100
    ASTHGLVINI IHSLCTCSQL HFSEETKQVL RLSLTEFSLP KFYLLFGISK 2150
    VKSAAVIAFR SSYRDRSFSP GSYERETFAL TSLETVTEAL LEIMEACMRD 2200
    IPTCKWLDQW TELAQRFAFQ YNPSLQPRAL VVFGCISKRV SHGQIKQIIR 2250
    ILSKALESCL KGPDTYNSQV LIEATVIALT KLQPLLNKDS PLHKALFWVA 2300
    VAVLQLDEVN LYSAGTALLE QNLHTLDSLR IFNDKSPEEV FMAIRNPLEW 2350
    HCKQMDHFVG LNFNSNFNFA LVGHLLKGYR HPSPAIVART VRILHTLLTL 2400
    VNKHRNCDKF EVNTQSVAYL AALLTVSEEV RSRCSLKHRK SLLLTDISME 2450
    NVPMDTYPIH HGDPSSRTLK ETQPWSSPRG SEGYLAATYP AVGQTSPRAR 2500
    KSMSLDMGQP SQANTKKLLG TRKSFDHLIS DTKAPKRQEM ESGITTPPKM 2550
    RRVAETDYEM ETQRISSSQQ HPHLRKVSVS ESNVLLDEEV LTDPKIQALL 2600
    LTVLATLVKY TTDEFDQRIL YEYLAEASVV FPKVFPLVHN LLDSKINTLL 2650
    SLCQDPNLLN PIHGIVQSVV YHEESPPQYQ TSYLQSFGFN GLWRFAGPFS 2700
    KQTQIPDYAE LIVKFLDALI DTYLPGIDEE TSEESLLTPT SPYPPALQSQ 2750
    LSITANLNLS NSMTSLATSQ HSPGIDKENV ELSPTTGHCN SGRTRHGSAS 2800
    QVQKQRSAGS FKRNSIKKIV 2820
    Length:2,820
    Mass (Da):317,083
    Last modified:May 1, 1997 - v1
    Checksum:iFC108487E86DA89F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45201 mRNA. Translation: BAA08141.1.
    PIRiJC5196.
    RefSeqiNP_036741.1. NM_012609.1.
    UniGeneiRn.10686.

    Genome annotation databases

    GeneIDi24592.
    KEGGirno:24592.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45201 mRNA. Translation: BAA08141.1 .
    PIRi JC5196.
    RefSeqi NP_036741.1. NM_012609.1.
    UniGenei Rn.10686.

    3D structure databases

    ProteinModelPortali P97526.
    SMRi P97526. Positions 1208-1531, 1562-1818.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246735. 2 interactions.
    IntActi P97526. 4 interactions.
    MINTi MINT-198504.
    STRINGi 10116.ENSRNOP00000049106.

    PTM databases

    PhosphoSitei P97526.

    Proteomic databases

    PaxDbi P97526.
    PRIDEi P97526.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24592.
    KEGGi rno:24592.

    Organism-specific databases

    CTDi 4763.
    RGDi 3168. Nf1.

    Phylogenomic databases

    eggNOGi COG5261.
    HOGENOMi HOG000047020.
    HOVERGENi HBG006486.
    KOi K08052.
    PhylomeDBi P97526.

    Miscellaneous databases

    NextBioi 603784.
    PROi P97526.

    Gene expression databases

    Genevestigatori P97526.

    Family and domain databases

    Gene3Di 1.10.506.10. 1 hit.
    1.25.10.10. 7 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR001251. CRAL-TRIO_dom.
    IPR028553. Neurofibromin.
    IPR001936. RasGAP.
    IPR023152. RasGAP_CS.
    IPR008936. Rho_GTPase_activation_prot.
    [Graphical view ]
    PANTHERi PTHR10194:SF60. PTHR10194:SF60. 1 hit.
    Pfami PF13716. CRAL_TRIO_2. 1 hit.
    PF00616. RasGAP. 1 hit.
    [Graphical view ]
    SMARTi SM00323. RasGAP. 1 hit.
    SM00516. SEC14. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    SSF48371. SSF48371. 8 hits.
    PROSITEi PS50191. CRAL_TRIO. 1 hit.
    PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
    PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential splicing of the neurofibromatosis type 1 (NF1) gene in rats: homologous splice variants in human are expressed in rat cells."
      Kyritsis A.P., Lee P.S., Mochizuki H., Nishi T., Levin V.A., Saya H.
      Int. J. Oncol. 1:149-152(1992)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain.
    2. "Differential tissue-specific expression of neurofibromin isoform mRNAs in rat."
      Suzuki H., Takahashi K., Yasumoto K., Fuse N., Shibahara S.
      J. Biochem. 120:1048-1054(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain.
    3. Bienvenut W.V., von Kriegsheim A.F., Kolch W.
      Submitted (MAR-2007) to UniProtKB
      Tissue: Pheochromocytoma.

    Entry informationi

    Entry nameiNF1_RAT
    AccessioniPrimary (citable) accession number: P97526
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 4, 2001
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3