Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neurofibromin

Gene

Nf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofibromin
Alternative name(s):
Neurofibromatosis-related protein NF-1
Gene namesi
Name:Nf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3168 Nf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000566672 – 2820NeurofibrominAdd BLAST2819

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei866PhosphoserineCombined sources1
Modified residuei878PhosphoserineBy similarity1
Modified residuei2169PhosphoserineCombined sources1
Modified residuei2448PhosphoserineBy similarity1
Modified residuei2495PhosphothreonineBy similarity1
Modified residuei2496PhosphoserineBy similarity1
Modified residuei2502PhosphoserineBy similarity1
Modified residuei2504PhosphoserineCombined sources1
Modified residuei2524PhosphoserineCombined sources1
Modified residuei2546PhosphothreonineBy similarity1
Modified residuei2578PhosphoserineBy similarity1
Modified residuei2783PhosphoserineBy similarity1
Modified residuei2798PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP97526
PRIDEiP97526

PTM databases

CarbonylDBiP97526
iPTMnetiP97526
PhosphoSitePlusiP97526

Interactioni

Subunit structurei

Interacts with HTR6 (By similarity).By similarity

GO - Molecular functioni

  • microtubule binding Source: MGI

Protein-protein interaction databases

BioGridi246735, 7 interactors
IntActiP97526, 4 interactors
MINTiP97526
STRINGi10116.ENSRNOP00000050393

Structurei

3D structure databases

ProteinModelPortaliP97526
SMRiP97526
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1237 – 1432Ras-GAPPROSITE-ProRule annotationAdd BLAST196
Domaini1561 – 1719CRAL-TRIOPROSITE-ProRule annotationAdd BLAST159

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1561 – 1818Lipid bindingBy similarityAdd BLAST258

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi2536 – 2552Bipartite nuclear localization signalBy similarityAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1354 – 1357Poly-Ser4

Domaini

Binds phospholipids via a region that includes the CRAL-TRIO domain. Binds primarily glycerophospholipids with monounsaturated C18:1 and/or C16:1 fatty acid moieties and a phosphatidylethanolamine or phosphatidylcholine headgroup. Has lesser affinity for lipids containing phosphatidylserine and phosphatidylinositol (By similarity).By similarity

Phylogenomic databases

eggNOGiKOG1826 Eukaryota
ENOG410XRPJ LUCA
HOGENOMiHOG000047020
HOVERGENiHBG006486
InParanoidiP97526
KOiK08052
PhylomeDBiP97526

Family and domain databases

CDDicd00170 SEC14, 1 hit
Gene3Di2.30.29.30, 1 hit
3.40.525.10, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR001251 CRAL-TRIO_dom
IPR036865 CRAL-TRIO_dom_sf
IPR011993 PH-like_dom_sf
IPR023152 RasGAP_CS
IPR001936 RasGAP_dom
IPR008936 Rho_GTPase_activation_prot
PfamiView protein in Pfam
PF13716 CRAL_TRIO_2, 1 hit
PF00616 RasGAP, 1 hit
SMARTiView protein in SMART
SM00323 RasGAP, 1 hit
SM00516 SEC14, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
SSF48371 SSF48371, 8 hits
PROSITEiView protein in PROSITE
PS50191 CRAL_TRIO, 1 hit
PS00509 RAS_GTPASE_ACTIV_1, 1 hit
PS50018 RAS_GTPASE_ACTIV_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAHRPVEWV QAVVSRFDEQ LPIKTGQQNT HTKVSTEHNK ECLINISKYK
60 70 80 90 100
FSLVISGLTT ILKNVNNMRI FGEAAEKNLY LSQLIILDTL EKCLAGQPKD
110 120 130 140 150
TMRLDETMLV KQLLPEICHF LHTCREGNQH AAELRNSASG VLFSLSCNNF
160 170 180 190 200
NAVFSRISTR LQELTVCSED NVDVHDIELL QYINVDCAKL KRLLKETAFK
210 220 230 240 250
FKALKKVAQL AVINSLEKAF WNWVENYPDE FTKLYQIPQT DMAECAGKLF
260 270 280 290 300
DLVDGFAEST KRKAAVWPLQ IILLILCPEI IQDISRDVVD ENNTNKKLFL
310 320 330 340 350
DSLRKALAGH GGSRQLTESA AIACVKLCKA STYINWEDNS VIFLLVQSMV
360 370 380 390 400
VDLKNLLFNP SKPFSRGSQP ADVDLMIDCL VSCFRISPHN NQHFKICLAQ
410 420 430 440 450
NSPSTFHYVL VNSLHRIITN SAWDWWPKID AVYCHSVELR NMFGETLHKA
460 470 480 490 500
VQGCGAHPAL RMAPSLTFKE KVTSLKFKEK PTDLEARSYK YLLLSMVKLI
510 520 530 540 550
HADPKLLLCN PRKQGPETQG STAELITGLV QLVPQSHMPE VAQEAMEALL
560 570 580 590 600
VLHQLDSIDL WNPDAPVETF WEISSQMLFY ICKKLTSHQM LSSTEILKWL
610 620 630 640 650
REILICRNKF LLKNKQADRS SCHSLYLYGV GCDLPASGNV TQMSVDHEES
660 670 680 690 700
LRTCAPGASL RKGRGNSSMD STAGCSGTPP ICRQAQTKLE VALYMFLWSP
710 720 730 740 750
DTEVVLVAMS CFRHLCEEAD IRCGVDEVSV HNFLPNYNTF MEFASVSNML
760 770 780 790 800
STGRAALQKR VMALLRRIEH PTAGNTEAWE DTHAKWEQAT KLILNYPKAK
810 820 830 840 850
MEDGQAAESL HKTIVKRRMS HVSGGGSIDL SDTDSLQEWI NMTGFLCALG
860 870 880 890 900
GVCLQQRSSS GLATYSPPMG PVSERKGSMI SVMSSEGNVD SPVSRFMDRL
910 920 930 940 950
LSLMVCNHEK VGLQIRTNVK DLVGLELSPA LYPMLFNKLK SAISKFFDSQ
960 970 980 990 1000
GQVLLTDSNT QFVEQTIAIM KNLLDNHTEG SSEHLGQASI ETMMLNLVRY
1010 1020 1030 1040 1050
VRVLGNMVHA IQIKTKLCQL VEVMMARRDD LSFCQEMKFR NKMVEYLTDW
1060 1070 1080 1090 1100
VMGTSNQAAD DDVKCLTRDL DQASMEAVVS LLAGLPLQPE EGDGVELMEA
1110 1120 1130 1140 1150
KSQLFLKYFT LFMNLLNDCS EVEDENAQTG GRKRGMSRRL ASLRHCTVLA
1160 1170 1180 1190 1200
MSNLLNANVD SGLMHSIGLG YHKDLQTRAT FMEVLTKILQ QGTEFDTLAE
1210 1220 1230 1240 1250
TVLADRFERL VELVTMMGDQ GELPIAMALA NVVPCSQWDE LARVLVTLFD
1260 1270 1280 1290 1300
SRHLLYQLLW NMFSKEVELA DSMQTLFRGN SLASKIMTFC FKVYGATYLQ
1310 1320 1330 1340 1350
KLLDPLLRII ITSSDWQHVS FEVDPTRLEP SESLEENQRN LLQMTEKFFH
1360 1370 1380 1390 1400
AIISSSSEFP SQLRSVCHCL YQVVSQRFPQ NSIGAVGSAM FLRFINPAIV
1410 1420 1430 1440 1450
SPYEAGILDK KPPPRIERGL KLMSKVLQSI ANHVLFTKEE HMRPFNDFVK
1460 1470 1480 1490 1500
SNFDLARRFF LDIASDCPTS DAVNHSLSFI SDGNVLALHR LLWNNQEKIG
1510 1520 1530 1540 1550
QYLSSNRDHK AVGRRPFDKM ATLLAYLGPP EHKPVADTHW SSLNLTSSKF
1560 1570 1580 1590 1600
EEFMTRHQVH EKEEFKALKT LSIFYQAGTS KAGNPIFYYV ARRFKTGQIN
1610 1620 1630 1640 1650
GDLLIYHVLL TLKPYYAKPY EIVVDLTHTG PSNRFKTDFL SKWFVVFPGF
1660 1670 1680 1690 1700
AYDNVSAVYI YNCNSWVREY TKYHERLLTG LKGSKRLIFI DCPGKLAEHI
1710 1720 1730 1740 1750
EHEQQKLPAA TLALEEDLKV FHNALKLAHK DTKVSIKVGS TAVQVTSAER
1760 1770 1780 1790 1800
TKVLGQSVFL NDIYYASEIE EICLVDENQF TLTIANQGTP LTFMHQECEA
1810 1820 1830 1840 1850
IVQSIIHIRT RWELSQPDSI PQHTKIRPKD VPGTLLNIAL LNLGSSDPSL
1860 1870 1880 1890 1900
RSAAYNLLCA LTCTFNLKIE GQLLETSGLC IPANNTLFIV SISKTLAANE
1910 1920 1930 1940 1950
PHLTLEFLEE CISGFSKSSI ELKHLCLEYM TPWLSNLVRF CKHNDDAKRQ
1960 1970 1980 1990 2000
RVTAILDKLI TMTINEKQMY PSIQAKIWGS LGQITDLLDV VLDSFIKTSA
2010 2020 2030 2040 2050
TGGLGSIKAE VMADTAVALA SGNVKLVSSK VIGRMCKIID KTCLSPTPTL
2060 2070 2080 2090 2100
EQHLMWDDIA ILARYMLMLS FNNSLDVAAH LPYLFHVVTF LVATGPLSLR
2110 2120 2130 2140 2150
ASTHGLVINI IHSLCTCSQL HFSEETKQVL RLSLTEFSLP KFYLLFGISK
2160 2170 2180 2190 2200
VKSAAVIAFR SSYRDRSFSP GSYERETFAL TSLETVTEAL LEIMEACMRD
2210 2220 2230 2240 2250
IPTCKWLDQW TELAQRFAFQ YNPSLQPRAL VVFGCISKRV SHGQIKQIIR
2260 2270 2280 2290 2300
ILSKALESCL KGPDTYNSQV LIEATVIALT KLQPLLNKDS PLHKALFWVA
2310 2320 2330 2340 2350
VAVLQLDEVN LYSAGTALLE QNLHTLDSLR IFNDKSPEEV FMAIRNPLEW
2360 2370 2380 2390 2400
HCKQMDHFVG LNFNSNFNFA LVGHLLKGYR HPSPAIVART VRILHTLLTL
2410 2420 2430 2440 2450
VNKHRNCDKF EVNTQSVAYL AALLTVSEEV RSRCSLKHRK SLLLTDISME
2460 2470 2480 2490 2500
NVPMDTYPIH HGDPSSRTLK ETQPWSSPRG SEGYLAATYP AVGQTSPRAR
2510 2520 2530 2540 2550
KSMSLDMGQP SQANTKKLLG TRKSFDHLIS DTKAPKRQEM ESGITTPPKM
2560 2570 2580 2590 2600
RRVAETDYEM ETQRISSSQQ HPHLRKVSVS ESNVLLDEEV LTDPKIQALL
2610 2620 2630 2640 2650
LTVLATLVKY TTDEFDQRIL YEYLAEASVV FPKVFPLVHN LLDSKINTLL
2660 2670 2680 2690 2700
SLCQDPNLLN PIHGIVQSVV YHEESPPQYQ TSYLQSFGFN GLWRFAGPFS
2710 2720 2730 2740 2750
KQTQIPDYAE LIVKFLDALI DTYLPGIDEE TSEESLLTPT SPYPPALQSQ
2760 2770 2780 2790 2800
LSITANLNLS NSMTSLATSQ HSPGIDKENV ELSPTTGHCN SGRTRHGSAS
2810 2820
QVQKQRSAGS FKRNSIKKIV
Length:2,820
Mass (Da):317,083
Last modified:May 1, 1997 - v1
Checksum:iFC108487E86DA89F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45201 mRNA Translation: BAA08141.1
PIRiJC5196
RefSeqiNP_036741.1, NM_012609.1
UniGeneiRn.10686

Genome annotation databases

GeneIDi24592
KEGGirno:24592

Similar proteinsi

Entry informationi

Entry nameiNF1_RAT
AccessioniPrimary (citable) accession number: P97526
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 1, 1997
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health