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P97526

- NF1_RAT

UniProt

P97526 - NF1_RAT

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Protein

Neurofibromin

Gene

Nf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity (By similarity).By similarity

GO - Molecular functioni

  1. microtubule binding Source: MGI
  2. phosphatidylcholine binding Source: UniProtKB
  3. phosphatidylethanolamine binding Source: UniProtKB
  4. Ras GTPase activator activity Source: UniProtKB
  5. syndecan binding Source: RGD

GO - Biological processi

  1. actin cytoskeleton organization Source: HGNC
  2. adrenal gland development Source: HGNC
  3. artery morphogenesis Source: HGNC
  4. brain development Source: HGNC
  5. camera-type eye morphogenesis Source: HGNC
  6. cell communication Source: HGNC
  7. cerebral cortex development Source: HGNC
  8. collagen fibril organization Source: HGNC
  9. extracellular matrix organization Source: HGNC
  10. forebrain astrocyte development Source: HGNC
  11. forebrain morphogenesis Source: HGNC
  12. heart development Source: HGNC
  13. liver development Source: HGNC
  14. MAPK cascade Source: HGNC
  15. metanephros development Source: HGNC
  16. myelination in peripheral nervous system Source: HGNC
  17. negative regulation of fibroblast proliferation Source: UniProtKB
  18. negative regulation of MAPK cascade Source: HGNC
  19. negative regulation of MAP kinase activity Source: HGNC
  20. negative regulation of neuroblast proliferation Source: HGNC
  21. negative regulation of oligodendrocyte differentiation Source: HGNC
  22. negative regulation of protein kinase activity Source: HGNC
  23. negative regulation of Ras protein signal transduction Source: RefGenome
  24. negative regulation of transcription factor import into nucleus Source: HGNC
  25. osteoblast differentiation Source: HGNC
  26. peripheral nervous system development Source: HGNC
  27. phosphatidylinositol 3-kinase signaling Source: HGNC
  28. pigmentation Source: HGNC
  29. positive regulation of adenylate cyclase activity Source: HGNC
  30. positive regulation of apoptotic process Source: HGNC
  31. positive regulation of neuron apoptotic process Source: HGNC
  32. positive regulation of Ras GTPase activity Source: UniProtKB
  33. Ras protein signal transduction Source: HGNC
  34. regulation of angiogenesis Source: HGNC
  35. regulation of bone resorption Source: HGNC
  36. regulation of cell-matrix adhesion Source: HGNC
  37. regulation of glial cell differentiation Source: HGNC
  38. regulation of neuron differentiation Source: RGD
  39. regulation of Ras GTPase activity Source: HGNC
  40. response to hypoxia Source: HGNC
  41. Schwann cell development Source: HGNC
  42. smooth muscle tissue development Source: HGNC
  43. spinal cord development Source: HGNC
  44. sympathetic nervous system development Source: HGNC
  45. visual learning Source: HGNC
  46. wound healing Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofibromin
Alternative name(s):
Neurofibromatosis-related protein NF-1
Gene namesi
Name:Nf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3168. Nf1.

Subcellular locationi

Nucleus By similarity. Nucleusnucleolus By similarity

GO - Cellular componenti

  1. axon Source: HGNC
  2. cytoplasm Source: HGNC
  3. dendrite Source: HGNC
  4. intrinsic component of the cytoplasmic side of the plasma membrane Source: RefGenome
  5. membrane Source: RGD
  6. neuron projection Source: RGD
  7. nucleus Source: HGNC
  8. postsynaptic density Source: RGD
  9. protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 28202819NeurofibrominPRO_0000056667Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei866 – 8661PhosphoserineBy similarity
Modified residuei878 – 8781PhosphoserineBy similarity
Modified residuei2169 – 21691PhosphoserineBy similarity
Modified residuei2496 – 24961PhosphoserineBy similarity
Modified residuei2502 – 25021PhosphoserineBy similarity
Modified residuei2524 – 25241PhosphoserineBy similarity
Modified residuei2798 – 27981PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP97526.
PRIDEiP97526.

PTM databases

PhosphoSiteiP97526.

Expressioni

Gene expression databases

GenevestigatoriP97526.

Interactioni

Protein-protein interaction databases

BioGridi246735. 2 interactions.
IntActiP97526. 4 interactions.
MINTiMINT-198504.
STRINGi10116.ENSRNOP00000049106.

Structurei

3D structure databases

ProteinModelPortaliP97526.
SMRiP97526. Positions 1208-1531, 1562-1818.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1237 – 1432196Ras-GAPPROSITE-ProRule annotationAdd
BLAST
Domaini1561 – 1719159CRAL-TRIOPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1561 – 1818258Lipid bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi2536 – 255217Bipartite nuclear localization signalBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1354 – 13574Poly-Ser

Domaini

Binds phospholipids via a region that includes the CRAL-TRIO domain. Binds primarily glycerophospholipids with monounsaturated C18:1 and/or C16:1 fatty acid moieties and a phosphatidylethanolamine or phosphatidylcholine headgroup. Has lesser affinity for lipids containing phosphatidylserine and phosphatidylinositol (By similarity).By similarity

Sequence similaritiesi

Contains 1 CRAL-TRIO domain.PROSITE-ProRule annotation
Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5261.
HOGENOMiHOG000047020.
HOVERGENiHBG006486.
InParanoidiP97526.
KOiK08052.
PhylomeDBiP97526.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
1.25.10.10. 7 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001251. CRAL-TRIO_dom.
IPR028553. Neurofibromin.
IPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PANTHERiPTHR10194:SF60. PTHR10194:SF60. 1 hit.
PfamiPF13716. CRAL_TRIO_2. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view]
SMARTiSM00323. RasGAP. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF48371. SSF48371. 8 hits.
PROSITEiPS50191. CRAL_TRIO. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97526-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAHRPVEWV QAVVSRFDEQ LPIKTGQQNT HTKVSTEHNK ECLINISKYK
60 70 80 90 100
FSLVISGLTT ILKNVNNMRI FGEAAEKNLY LSQLIILDTL EKCLAGQPKD
110 120 130 140 150
TMRLDETMLV KQLLPEICHF LHTCREGNQH AAELRNSASG VLFSLSCNNF
160 170 180 190 200
NAVFSRISTR LQELTVCSED NVDVHDIELL QYINVDCAKL KRLLKETAFK
210 220 230 240 250
FKALKKVAQL AVINSLEKAF WNWVENYPDE FTKLYQIPQT DMAECAGKLF
260 270 280 290 300
DLVDGFAEST KRKAAVWPLQ IILLILCPEI IQDISRDVVD ENNTNKKLFL
310 320 330 340 350
DSLRKALAGH GGSRQLTESA AIACVKLCKA STYINWEDNS VIFLLVQSMV
360 370 380 390 400
VDLKNLLFNP SKPFSRGSQP ADVDLMIDCL VSCFRISPHN NQHFKICLAQ
410 420 430 440 450
NSPSTFHYVL VNSLHRIITN SAWDWWPKID AVYCHSVELR NMFGETLHKA
460 470 480 490 500
VQGCGAHPAL RMAPSLTFKE KVTSLKFKEK PTDLEARSYK YLLLSMVKLI
510 520 530 540 550
HADPKLLLCN PRKQGPETQG STAELITGLV QLVPQSHMPE VAQEAMEALL
560 570 580 590 600
VLHQLDSIDL WNPDAPVETF WEISSQMLFY ICKKLTSHQM LSSTEILKWL
610 620 630 640 650
REILICRNKF LLKNKQADRS SCHSLYLYGV GCDLPASGNV TQMSVDHEES
660 670 680 690 700
LRTCAPGASL RKGRGNSSMD STAGCSGTPP ICRQAQTKLE VALYMFLWSP
710 720 730 740 750
DTEVVLVAMS CFRHLCEEAD IRCGVDEVSV HNFLPNYNTF MEFASVSNML
760 770 780 790 800
STGRAALQKR VMALLRRIEH PTAGNTEAWE DTHAKWEQAT KLILNYPKAK
810 820 830 840 850
MEDGQAAESL HKTIVKRRMS HVSGGGSIDL SDTDSLQEWI NMTGFLCALG
860 870 880 890 900
GVCLQQRSSS GLATYSPPMG PVSERKGSMI SVMSSEGNVD SPVSRFMDRL
910 920 930 940 950
LSLMVCNHEK VGLQIRTNVK DLVGLELSPA LYPMLFNKLK SAISKFFDSQ
960 970 980 990 1000
GQVLLTDSNT QFVEQTIAIM KNLLDNHTEG SSEHLGQASI ETMMLNLVRY
1010 1020 1030 1040 1050
VRVLGNMVHA IQIKTKLCQL VEVMMARRDD LSFCQEMKFR NKMVEYLTDW
1060 1070 1080 1090 1100
VMGTSNQAAD DDVKCLTRDL DQASMEAVVS LLAGLPLQPE EGDGVELMEA
1110 1120 1130 1140 1150
KSQLFLKYFT LFMNLLNDCS EVEDENAQTG GRKRGMSRRL ASLRHCTVLA
1160 1170 1180 1190 1200
MSNLLNANVD SGLMHSIGLG YHKDLQTRAT FMEVLTKILQ QGTEFDTLAE
1210 1220 1230 1240 1250
TVLADRFERL VELVTMMGDQ GELPIAMALA NVVPCSQWDE LARVLVTLFD
1260 1270 1280 1290 1300
SRHLLYQLLW NMFSKEVELA DSMQTLFRGN SLASKIMTFC FKVYGATYLQ
1310 1320 1330 1340 1350
KLLDPLLRII ITSSDWQHVS FEVDPTRLEP SESLEENQRN LLQMTEKFFH
1360 1370 1380 1390 1400
AIISSSSEFP SQLRSVCHCL YQVVSQRFPQ NSIGAVGSAM FLRFINPAIV
1410 1420 1430 1440 1450
SPYEAGILDK KPPPRIERGL KLMSKVLQSI ANHVLFTKEE HMRPFNDFVK
1460 1470 1480 1490 1500
SNFDLARRFF LDIASDCPTS DAVNHSLSFI SDGNVLALHR LLWNNQEKIG
1510 1520 1530 1540 1550
QYLSSNRDHK AVGRRPFDKM ATLLAYLGPP EHKPVADTHW SSLNLTSSKF
1560 1570 1580 1590 1600
EEFMTRHQVH EKEEFKALKT LSIFYQAGTS KAGNPIFYYV ARRFKTGQIN
1610 1620 1630 1640 1650
GDLLIYHVLL TLKPYYAKPY EIVVDLTHTG PSNRFKTDFL SKWFVVFPGF
1660 1670 1680 1690 1700
AYDNVSAVYI YNCNSWVREY TKYHERLLTG LKGSKRLIFI DCPGKLAEHI
1710 1720 1730 1740 1750
EHEQQKLPAA TLALEEDLKV FHNALKLAHK DTKVSIKVGS TAVQVTSAER
1760 1770 1780 1790 1800
TKVLGQSVFL NDIYYASEIE EICLVDENQF TLTIANQGTP LTFMHQECEA
1810 1820 1830 1840 1850
IVQSIIHIRT RWELSQPDSI PQHTKIRPKD VPGTLLNIAL LNLGSSDPSL
1860 1870 1880 1890 1900
RSAAYNLLCA LTCTFNLKIE GQLLETSGLC IPANNTLFIV SISKTLAANE
1910 1920 1930 1940 1950
PHLTLEFLEE CISGFSKSSI ELKHLCLEYM TPWLSNLVRF CKHNDDAKRQ
1960 1970 1980 1990 2000
RVTAILDKLI TMTINEKQMY PSIQAKIWGS LGQITDLLDV VLDSFIKTSA
2010 2020 2030 2040 2050
TGGLGSIKAE VMADTAVALA SGNVKLVSSK VIGRMCKIID KTCLSPTPTL
2060 2070 2080 2090 2100
EQHLMWDDIA ILARYMLMLS FNNSLDVAAH LPYLFHVVTF LVATGPLSLR
2110 2120 2130 2140 2150
ASTHGLVINI IHSLCTCSQL HFSEETKQVL RLSLTEFSLP KFYLLFGISK
2160 2170 2180 2190 2200
VKSAAVIAFR SSYRDRSFSP GSYERETFAL TSLETVTEAL LEIMEACMRD
2210 2220 2230 2240 2250
IPTCKWLDQW TELAQRFAFQ YNPSLQPRAL VVFGCISKRV SHGQIKQIIR
2260 2270 2280 2290 2300
ILSKALESCL KGPDTYNSQV LIEATVIALT KLQPLLNKDS PLHKALFWVA
2310 2320 2330 2340 2350
VAVLQLDEVN LYSAGTALLE QNLHTLDSLR IFNDKSPEEV FMAIRNPLEW
2360 2370 2380 2390 2400
HCKQMDHFVG LNFNSNFNFA LVGHLLKGYR HPSPAIVART VRILHTLLTL
2410 2420 2430 2440 2450
VNKHRNCDKF EVNTQSVAYL AALLTVSEEV RSRCSLKHRK SLLLTDISME
2460 2470 2480 2490 2500
NVPMDTYPIH HGDPSSRTLK ETQPWSSPRG SEGYLAATYP AVGQTSPRAR
2510 2520 2530 2540 2550
KSMSLDMGQP SQANTKKLLG TRKSFDHLIS DTKAPKRQEM ESGITTPPKM
2560 2570 2580 2590 2600
RRVAETDYEM ETQRISSSQQ HPHLRKVSVS ESNVLLDEEV LTDPKIQALL
2610 2620 2630 2640 2650
LTVLATLVKY TTDEFDQRIL YEYLAEASVV FPKVFPLVHN LLDSKINTLL
2660 2670 2680 2690 2700
SLCQDPNLLN PIHGIVQSVV YHEESPPQYQ TSYLQSFGFN GLWRFAGPFS
2710 2720 2730 2740 2750
KQTQIPDYAE LIVKFLDALI DTYLPGIDEE TSEESLLTPT SPYPPALQSQ
2760 2770 2780 2790 2800
LSITANLNLS NSMTSLATSQ HSPGIDKENV ELSPTTGHCN SGRTRHGSAS
2810 2820
QVQKQRSAGS FKRNSIKKIV
Length:2,820
Mass (Da):317,083
Last modified:May 1, 1997 - v1
Checksum:iFC108487E86DA89F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45201 mRNA. Translation: BAA08141.1.
PIRiJC5196.
RefSeqiNP_036741.1. NM_012609.1.
UniGeneiRn.10686.

Genome annotation databases

GeneIDi24592.
KEGGirno:24592.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45201 mRNA. Translation: BAA08141.1 .
PIRi JC5196.
RefSeqi NP_036741.1. NM_012609.1.
UniGenei Rn.10686.

3D structure databases

ProteinModelPortali P97526.
SMRi P97526. Positions 1208-1531, 1562-1818.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246735. 2 interactions.
IntActi P97526. 4 interactions.
MINTi MINT-198504.
STRINGi 10116.ENSRNOP00000049106.

PTM databases

PhosphoSitei P97526.

Proteomic databases

PaxDbi P97526.
PRIDEi P97526.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24592.
KEGGi rno:24592.

Organism-specific databases

CTDi 4763.
RGDi 3168. Nf1.

Phylogenomic databases

eggNOGi COG5261.
HOGENOMi HOG000047020.
HOVERGENi HBG006486.
InParanoidi P97526.
KOi K08052.
PhylomeDBi P97526.

Miscellaneous databases

NextBioi 603784.
PROi P97526.

Gene expression databases

Genevestigatori P97526.

Family and domain databases

Gene3Di 1.10.506.10. 1 hit.
1.25.10.10. 7 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001251. CRAL-TRIO_dom.
IPR028553. Neurofibromin.
IPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view ]
PANTHERi PTHR10194:SF60. PTHR10194:SF60. 1 hit.
Pfami PF13716. CRAL_TRIO_2. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view ]
SMARTi SM00323. RasGAP. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
SSF48371. SSF48371. 8 hits.
PROSITEi PS50191. CRAL_TRIO. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Differential splicing of the neurofibromatosis type 1 (NF1) gene in rats: homologous splice variants in human are expressed in rat cells."
    Kyritsis A.P., Lee P.S., Mochizuki H., Nishi T., Levin V.A., Saya H.
    Int. J. Oncol. 1:149-152(1992)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  2. "Differential tissue-specific expression of neurofibromin isoform mRNAs in rat."
    Suzuki H., Takahashi K., Yasumoto K., Fuse N., Shibahara S.
    J. Biochem. 120:1048-1054(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  3. Bienvenut W.V., von Kriegsheim A.F., Kolch W.
    Submitted (MAR-2007) to UniProtKB
    Tissue: Pheochromocytoma.

Entry informationi

Entry nameiNF1_RAT
AccessioniPrimary (citable) accession number: P97526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3