P97526 (NF1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neurofibromin

Alternative name(s):
Neurofibromatosis-related protein NF-1

Gene names

Name:

Nf1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	2820 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity By similarity.
Sequence similarities	Contains 1 CRAL-TRIO domain. Contains 1 Ras-GAP domain.

Ontologies

Keywords
Molecular function	GTPase activation
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade Inferred from sequence or structural similarity. Source: HGNC Ras protein signal transduction Inferred from sequence or structural similarity. Source: HGNC actin cytoskeleton organization Inferred from sequence or structural similarity. Source: HGNC adrenal gland development Inferred from sequence or structural similarity. Source: HGNC artery morphogenesis Inferred from sequence or structural similarity. Source: HGNC camera-type eye morphogenesis Inferred from sequence or structural similarity. Source: HGNC cerebral cortex development Inferred from sequence or structural similarity. Source: HGNC collagen fibril organization Inferred from sequence or structural similarity. Source: HGNC forebrain astrocyte development Inferred from sequence or structural similarity. Source: HGNC forebrain morphogenesis Inferred from sequence or structural similarity. Source: HGNC heart development Inferred from sequence or structural similarity. Source: HGNC liver development Inferred from sequence or structural similarity. Source: HGNC metanephros development Inferred from sequence or structural similarity. Source: HGNC myelination in peripheral nervous system Inferred from sequence or structural similarity. Source: HGNC negative regulation of MAP kinase activity Inferred from sequence or structural similarity. Source: HGNC negative regulation of Ras protein signal transduction Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of neuroblast proliferation Inferred from sequence or structural similarity. Source: HGNC negative regulation of oligodendrocyte differentiation Inferred from sequence or structural similarity. Source: HGNC negative regulation of transcription factor import into nucleus Inferred from sequence or structural similarity. Source: HGNC osteoblast differentiation Inferred from sequence or structural similarity. Source: HGNC phosphatidylinositol 3-kinase cascade Inferred from sequence or structural similarity. Source: HGNC pigmentation Inferred from sequence or structural similarity. Source: HGNC positive regulation of adenylate cyclase activity Inferred from sequence or structural similarity. Source: HGNC positive regulation of neuron apoptotic process Inferred from sequence or structural similarity. Source: HGNC regulation of angiogenesis Inferred from sequence or structural similarity. Source: HGNC regulation of bone resorption Inferred from sequence or structural similarity. Source: HGNC regulation of cell-matrix adhesion Inferred from sequence or structural similarity. Source: HGNC regulation of neuron differentiation Inferred from mutant phenotype PubMed 18218617. Source: RGD response to hypoxia Inferred from sequence or structural similarity. Source: HGNC smooth muscle tissue development Inferred from sequence or structural similarity. Source: HGNC spinal cord development Inferred from sequence or structural similarity. Source: HGNC sympathetic nervous system development Inferred from sequence or structural similarity. Source: HGNC visual learning Inferred from sequence or structural similarity. Source: HGNC wound healing Inferred from sequence or structural similarity. Source: HGNC
Cellular_component	axon Inferred from direct assay PubMed 1550670. Source: HGNC cytoplasm Inferred from direct assay PubMed 1550670. Source: HGNC intrinsic to internal side of plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome nucleus Inferred from direct assay PubMed 1550670. Source: HGNC postsynaptic density Inferred from direct assay PubMed 11356864. Source: RGD protein complex Inferred from direct assay PubMed 11356864. Source: RGD
Molecular_function	Ras GTPase activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome microtubule binding Inferred from direct assay PubMed 7568895. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 2820	2819	Neurofibromin		PRO_0000056667
Regions
	Domain	1237 – 1432	196	Ras-GAP
	Domain	1561 – 1719	159	CRAL-TRIO
	Compositional bias	1354 – 1357	4	Poly-Ser
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.3
	Modified residue	866	1	Phosphoserine By similarity
	Modified residue	878	1	Phosphoserine By similarity
	Modified residue	2169	1	Phosphoserine By similarity
	Modified residue	2495	1	Phosphothreonine By similarity
	Modified residue	2496	1	Phosphoserine By similarity
	Modified residue	2502	1	Phosphoserine By similarity
	Modified residue	2524	1	Phosphoserine By similarity
	Modified residue	2798	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97526 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: FC108487E86DA89F	FASTA	2,820	317,083
	10 20 30 40 50 60 MAAHRPVEWV QAVVSRFDEQ LPIKTGQQNT HTKVSTEHNK ECLINISKYK FSLVISGLTT 70 80 90 100 110 120 ILKNVNNMRI FGEAAEKNLY LSQLIILDTL EKCLAGQPKD TMRLDETMLV KQLLPEICHF 130 140 150 160 170 180 LHTCREGNQH AAELRNSASG VLFSLSCNNF NAVFSRISTR LQELTVCSED NVDVHDIELL 190 200 210 220 230 240 QYINVDCAKL KRLLKETAFK FKALKKVAQL AVINSLEKAF WNWVENYPDE FTKLYQIPQT 250 260 270 280 290 300 DMAECAGKLF DLVDGFAEST KRKAAVWPLQ IILLILCPEI IQDISRDVVD ENNTNKKLFL 310 320 330 340 350 360 DSLRKALAGH GGSRQLTESA AIACVKLCKA STYINWEDNS VIFLLVQSMV VDLKNLLFNP 370 380 390 400 410 420 SKPFSRGSQP ADVDLMIDCL VSCFRISPHN NQHFKICLAQ NSPSTFHYVL VNSLHRIITN 430 440 450 460 470 480 SAWDWWPKID AVYCHSVELR NMFGETLHKA VQGCGAHPAL RMAPSLTFKE KVTSLKFKEK 490 500 510 520 530 540 PTDLEARSYK YLLLSMVKLI HADPKLLLCN PRKQGPETQG STAELITGLV QLVPQSHMPE 550 560 570 580 590 600 VAQEAMEALL VLHQLDSIDL WNPDAPVETF WEISSQMLFY ICKKLTSHQM LSSTEILKWL 610 620 630 640 650 660 REILICRNKF LLKNKQADRS SCHSLYLYGV GCDLPASGNV TQMSVDHEES LRTCAPGASL 670 680 690 700 710 720 RKGRGNSSMD STAGCSGTPP ICRQAQTKLE VALYMFLWSP DTEVVLVAMS CFRHLCEEAD 730 740 750 760 770 780 IRCGVDEVSV HNFLPNYNTF MEFASVSNML STGRAALQKR VMALLRRIEH PTAGNTEAWE 790 800 810 820 830 840 DTHAKWEQAT KLILNYPKAK MEDGQAAESL HKTIVKRRMS HVSGGGSIDL SDTDSLQEWI 850 860 870 880 890 900 NMTGFLCALG GVCLQQRSSS GLATYSPPMG PVSERKGSMI SVMSSEGNVD SPVSRFMDRL 910 920 930 940 950 960 LSLMVCNHEK VGLQIRTNVK DLVGLELSPA LYPMLFNKLK SAISKFFDSQ GQVLLTDSNT 970 980 990 1000 1010 1020 QFVEQTIAIM KNLLDNHTEG SSEHLGQASI ETMMLNLVRY VRVLGNMVHA IQIKTKLCQL 1030 1040 1050 1060 1070 1080 VEVMMARRDD LSFCQEMKFR NKMVEYLTDW VMGTSNQAAD DDVKCLTRDL DQASMEAVVS 1090 1100 1110 1120 1130 1140 LLAGLPLQPE EGDGVELMEA KSQLFLKYFT LFMNLLNDCS EVEDENAQTG GRKRGMSRRL 1150 1160 1170 1180 1190 1200 ASLRHCTVLA MSNLLNANVD SGLMHSIGLG YHKDLQTRAT FMEVLTKILQ QGTEFDTLAE 1210 1220 1230 1240 1250 1260 TVLADRFERL VELVTMMGDQ GELPIAMALA NVVPCSQWDE LARVLVTLFD SRHLLYQLLW 1270 1280 1290 1300 1310 1320 NMFSKEVELA DSMQTLFRGN SLASKIMTFC FKVYGATYLQ KLLDPLLRII ITSSDWQHVS 1330 1340 1350 1360 1370 1380 FEVDPTRLEP SESLEENQRN LLQMTEKFFH AIISSSSEFP SQLRSVCHCL YQVVSQRFPQ 1390 1400 1410 1420 1430 1440 NSIGAVGSAM FLRFINPAIV SPYEAGILDK KPPPRIERGL KLMSKVLQSI ANHVLFTKEE 1450 1460 1470 1480 1490 1500 HMRPFNDFVK SNFDLARRFF LDIASDCPTS DAVNHSLSFI SDGNVLALHR LLWNNQEKIG 1510 1520 1530 1540 1550 1560 QYLSSNRDHK AVGRRPFDKM ATLLAYLGPP EHKPVADTHW SSLNLTSSKF EEFMTRHQVH 1570 1580 1590 1600 1610 1620 EKEEFKALKT LSIFYQAGTS KAGNPIFYYV ARRFKTGQIN GDLLIYHVLL TLKPYYAKPY 1630 1640 1650 1660 1670 1680 EIVVDLTHTG PSNRFKTDFL SKWFVVFPGF AYDNVSAVYI YNCNSWVREY TKYHERLLTG 1690 1700 1710 1720 1730 1740 LKGSKRLIFI DCPGKLAEHI EHEQQKLPAA TLALEEDLKV FHNALKLAHK DTKVSIKVGS 1750 1760 1770 1780 1790 1800 TAVQVTSAER TKVLGQSVFL NDIYYASEIE EICLVDENQF TLTIANQGTP LTFMHQECEA 1810 1820 1830 1840 1850 1860 IVQSIIHIRT RWELSQPDSI PQHTKIRPKD VPGTLLNIAL LNLGSSDPSL RSAAYNLLCA 1870 1880 1890 1900 1910 1920 LTCTFNLKIE GQLLETSGLC IPANNTLFIV SISKTLAANE PHLTLEFLEE CISGFSKSSI 1930 1940 1950 1960 1970 1980 ELKHLCLEYM TPWLSNLVRF CKHNDDAKRQ RVTAILDKLI TMTINEKQMY PSIQAKIWGS 1990 2000 2010 2020 2030 2040 LGQITDLLDV VLDSFIKTSA TGGLGSIKAE VMADTAVALA SGNVKLVSSK VIGRMCKIID 2050 2060 2070 2080 2090 2100 KTCLSPTPTL EQHLMWDDIA ILARYMLMLS FNNSLDVAAH LPYLFHVVTF LVATGPLSLR 2110 2120 2130 2140 2150 2160 ASTHGLVINI IHSLCTCSQL HFSEETKQVL RLSLTEFSLP KFYLLFGISK VKSAAVIAFR 2170 2180 2190 2200 2210 2220 SSYRDRSFSP GSYERETFAL TSLETVTEAL LEIMEACMRD IPTCKWLDQW TELAQRFAFQ 2230 2240 2250 2260 2270 2280 YNPSLQPRAL VVFGCISKRV SHGQIKQIIR ILSKALESCL KGPDTYNSQV LIEATVIALT 2290 2300 2310 2320 2330 2340 KLQPLLNKDS PLHKALFWVA VAVLQLDEVN LYSAGTALLE QNLHTLDSLR IFNDKSPEEV 2350 2360 2370 2380 2390 2400 FMAIRNPLEW HCKQMDHFVG LNFNSNFNFA LVGHLLKGYR HPSPAIVART VRILHTLLTL 2410 2420 2430 2440 2450 2460 VNKHRNCDKF EVNTQSVAYL AALLTVSEEV RSRCSLKHRK SLLLTDISME NVPMDTYPIH 2470 2480 2490 2500 2510 2520 HGDPSSRTLK ETQPWSSPRG SEGYLAATYP AVGQTSPRAR KSMSLDMGQP SQANTKKLLG 2530 2540 2550 2560 2570 2580 TRKSFDHLIS DTKAPKRQEM ESGITTPPKM RRVAETDYEM ETQRISSSQQ HPHLRKVSVS 2590 2600 2610 2620 2630 2640 ESNVLLDEEV LTDPKIQALL LTVLATLVKY TTDEFDQRIL YEYLAEASVV FPKVFPLVHN 2650 2660 2670 2680 2690 2700 LLDSKINTLL SLCQDPNLLN PIHGIVQSVV YHEESPPQYQ TSYLQSFGFN GLWRFAGPFS 2710 2720 2730 2740 2750 2760 KQTQIPDYAE LIVKFLDALI DTYLPGIDEE TSEESLLTPT SPYPPALQSQ LSITANLNLS 2770 2780 2790 2800 2810 2820 NSMTSLATSQ HSPGIDKENV ELSPTTGHCN SGRTRHGSAS QVQKQRSAGS FKRNSIKKIV

References

[1]	"Differential splicing of the neurofibromatosis type 1 (NF1) gene in rats: homologous splice variants in human are expressed in rat cells." Kyritsis A.P., Lee P.S., Mochizuki H., Nishi T., Levin V.A., Saya H. Int. J. Oncol. 1:149-152(1992) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.
[2]	"Differential tissue-specific expression of neurofibromin isoform mRNAs in rat." Suzuki H., Takahashi K., Yasumoto K., Fuse N., Shibahara S. J. Biochem. 120:1048-1054(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.
[3]	Bienvenut W.V., von Kriegsheim A.F., Kolch W. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-24; 51-63; 78-92; 104-111; 207-218; 249-262; 298-305; 355-366; 462-469; 491-498; 767-785; 792-798; 972-999; 1003-1014; 1179-1209; 1244-1252; 1266-1278; 1293-1327; 1340-1364; 1394-1410; 1426-1438; 1451-1457; 1491-1507; 1550-1556; 1570-1592; 1619-1634; 1707-1726; 1738-1750; 1810-1825; 1830-1851; 1952-1976; 1998-2025; 2132-2150; 2153-2160; 2167-2175; 2206-2228; 2262-2281; 2331-2345; 2468-2498; 2502-2516; 2596-2618 AND 2702-2714, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Pheochromocytoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D45201 mRNA. Translation: BAA08141.1.
IPI	IPI00205419.
PIR	JC5196.
RefSeq	NP_036741.1. NM_012609.1.
UniGene	Rn.10686.
3D structure databases
ProteinModelPortal	P97526.
SMR	P97526. Positions 1208-1531, 1562-1818.
ModBase	Search...
Protein-protein interaction databases
IntAct	P97526. 3 interactions.
MINT	MINT-198504.
STRING	10116.ENSRNOP00000049106.
PTM databases
PhosphoSite	P97526.
Proteomic databases
PaxDb	P97526.
PRIDE	P97526.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24592.
KEGG	rno:24592.
Organism-specific databases
CTD	4763.
RGD	3168. Nf1.
Phylogenomic databases
eggNOG	COG5261.
HOGENOM	HOG000047020.
HOVERGEN	HBG006486.
KO	K08052.
Gene expression databases
ArrayExpress	P97526.
Genevestigator	P97526.
Family and domain databases
Gene3D	1.10.506.10. 1 hit. 1.25.10.10. 7 hits.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR001251. CRAL-TRIO_dom. IPR001936. RasGAP. IPR023152. RasGAP_CS. IPR008936. Rho_GTPase_activation_prot. [Graphical view]
Pfam	PF00616. RasGAP. 1 hit. [Graphical view]
SMART	SM00323. RasGAP. 1 hit. SM00516. SEC14. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50191. CRAL_TRIO. 1 hit. PS00509. RAS_GTPASE_ACTIV_1. 1 hit. PS50018. RAS_GTPASE_ACTIV_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603784.

Entry information

Entry name

NF1_RAT

Accession

Primary (citable) accession number: P97526

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program