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Protein

Very long-chain acyl-CoA synthetase

Gene

Slc27a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetase probably involved in bile acid metabolism. Proposed to activate C27 precursors of bile acids to their CoA thioesters derivatives before side chain cleavage via peroxisomal beta-oxidation occurs. In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Does not utilize C24 bile acids as substrates. In vitro, also activates long- and branched-chain fatty acids and may have additional roles in fatty acid metabolism. May be involved in translocation of long-chain fatty acids (LFCA) across membranes (By similarity).By similarity

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
ATP + a very-long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi222 – 233AMPSequence analysisAdd BLAST12

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • decanoate-CoA ligase activity Source: UniProtKB-EC
  • long-chain fatty acid-CoA ligase activity Source: RGD
  • very long-chain fatty acid-CoA ligase activity Source: UniProtKB

GO - Biological processi

  • fatty acid transport Source: InterPro
  • long-chain fatty acid metabolic process Source: UniProtKB

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Very long-chain acyl-CoA synthetase (EC:6.2.1.-)
Short name:
VLACS
Short name:
VLCS
Alternative name(s):
Fatty acid transport protein 2
Short name:
FATP-2
Fatty-acid-coenzyme A ligase, very long-chain 1
Long-chain-fatty-acid--CoA ligase (EC:6.2.1.3)
Solute carrier family 27 member 2
THCA-CoA ligase
Very long-chain-fatty-acid-CoA ligase
Gene namesi
Name:Slc27a2
Synonyms:Acsvl1, Facvl1, Fatp2, Vlacs, Vlcs
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi71103 Slc27a2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4LumenalBy similarity4
Transmembranei5 – 27HelicalSequence analysisAdd BLAST23
Topological domaini28 – 106CytoplasmicSequence analysisAdd BLAST79
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Topological domaini128 – 267LumenalSequence analysisAdd BLAST140
Transmembranei268 – 288HelicalSequence analysisAdd BLAST21
Topological domaini289 – 620CytoplasmicBy similarityAdd BLAST332

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001932061 – 620Very long-chain acyl-CoA synthetaseAdd BLAST620

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei291N6-acetyllysineBy similarity1
Modified residuei577PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP97524

PTM databases

iPTMnetiP97524
PhosphoSitePlusiP97524

Interactioni

Protein-protein interaction databases

BioGridi249304, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliP97524
SMRiP97524
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000044189
HOVERGENiHBG005642
InParanoidiP97524
KOiK08746
PhylomeDBiP97524

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR030305 FATP2
PANTHERiPTHR43107:SF13 PTHR43107:SF13, 1 hit
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

P97524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPVLYTGLA GLLLLPLLLT CCCPYLLQDV RFFLQLANMA RQVRSYRQRR
60 70 80 90 100
PVRTILHVFL EQARKTPHKP FLLFRDETLT YAQVDRRSNQ VARALHDHLG
110 120 130 140 150
LRQGDCVALF MGNEPAYVWL WLGLLKLGCP MACLNYNIRA KSLLHCFQCC
160 170 180 190 200
GAKVLLASPE LHEAVEEVLP TLKKEGVSVF YVSRTSNTNG VDTVLDKVDG
210 220 230 240 250
VSADPIPESW RSEVTFTTPA VYIYTSGTTG LPKAATINHH RLWYGTSLAL
260 270 280 290 300
RSGIKAHDVI YTTMPLYHSA ALMIGLHGCI VVGATFALRS KFSASQFWDD
310 320 330 340 350
CRKYNATVIQ YIGELLRYLC NTPQKPNDRD HKVKIALGNG LRGDVWREFI
360 370 380 390 400
KRFGDIHIYE FYASTEGNIG FMNYPRKIGA VGRENYLQKK VVRHELIKYD
410 420 430 440 450
VEKDEPVRDA NGYCIKVPKG EVGLLICKIT ELTPFFGYAG GKTQTEKKKL
460 470 480 490 500
RDVFKKGDVY FNSGDLLMID RENFIYFHDR VGDTFRWKGE NVATTEVADI
510 520 530 540 550
VGLVDFVEEV NVYGVPVPGH EGRIGMASIK MKENYEFNGK KLFQHISEYL
560 570 580 590 600
PSYSRPRFLR IQDTIEITGT FKHRKVTLME EGFNPSVIKD TLYFMDDTEK
610 620
TYVPMTEDIY NAIIDKTLKL
Length:620
Mass (Da):70,694
Last modified:May 1, 1997 - v1
Checksum:i6CF9362DC3805526
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85100 mRNA Translation: BAA12722.1
RefSeqiNP_113924.1, NM_031736.1
UniGeneiRn.3608

Genome annotation databases

GeneIDi65192
KEGGirno:65192
UCSCiRGD:71103 rat

Similar proteinsi

Entry informationi

Entry nameiS27A2_RAT
AccessioniPrimary (citable) accession number: P97524
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: May 23, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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