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Protein

Cytoplasmic tyrosine-protein kinase BMX

Gene

Bmx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

TEK and vascular endothelial growth factor receptor 1 (FLT1) stimulate BMX tyrosine kinase activity. Activated by integrins through the mediation of PTK2/FAK1 (By similarity). Activated by TNF through the mediation of TNFRSF1B (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi121ZincBy similarity1
Metal bindingi132ZincBy similarity1
Metal bindingi133ZincBy similarity1
Metal bindingi143ZincBy similarity1
Binding sitei421ATPPROSITE-ProRule annotation1
Active sitei512Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri113 – 149Btk-typePROSITE-ProRule annotationAdd BLAST37
Nucleotide bindingi399 – 407ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Apoptosis, Cell adhesion

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-111465. Apoptotic cleavage of cellular proteins.
R-MMU-1660499. Synthesis of PIPs at the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic tyrosine-protein kinase BMX (EC:2.7.10.2)
Alternative name(s):
Bone marrow tyrosine kinase gene in chromosome X protein homolog
Gene namesi
Name:Bmx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1101778. Bmx.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Localizes to the edges of spreading cells when complexed with BCAR1.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2034793.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880641 – 651Cytoplasmic tyrosine-protein kinase BMXAdd BLAST651

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei542Phosphotyrosine; by SRC and autocatalysisBy similarity1

Post-translational modificationi

Phosphorylated in response to protein I/II and to LPS. Phosphorylation at Tyr-542 by SRC and by autocatalysis leads to activation and is required for STAT3 phosphorylation by BMX.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP97504.
PaxDbiP97504.
PRIDEiP97504.

PTM databases

iPTMnetiP97504.
PhosphoSitePlusiP97504.

Expressioni

Tissue specificityi

Specifically expressed in the endocardium of the developing heart as well as in the endocardium of the left ventricle and in the endothelium of large arteries in adult mice.1 Publication

Gene expression databases

BgeeiENSMUSG00000031377.
CleanExiMM_BMX.
ExpressionAtlasiP97504. baseline and differential.
GenevisibleiP97504. MM.

Interactioni

Subunit structurei

Interacts with BCAR1, CAV1, MYD88, PTK2/FAK1, RUFY1, RUFY2, STAT3, TIRAP and TNFRSF1B.By similarity

Protein-protein interaction databases

IntActiP97504. 1 interactor.
STRINGi10090.ENSMUSP00000107884.

Chemistry databases

BindingDBiP97504.

Structurei

3D structure databases

ProteinModelPortaliP97504.
SMRiP97504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 111PHPROSITE-ProRule annotationAdd BLAST108
Domaini272 – 368SH2PROSITE-ProRule annotationAdd BLAST97
Domaini393 – 646Protein kinasePROSITE-ProRule annotationAdd BLAST254

Domaini

SH2 domain mediates interaction with RUFY1.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.PROSITE-ProRule annotation
Contains 1 Btk-type zinc finger.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri113 – 149Btk-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

SH2 domain, Zinc-finger

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000119011.
HOGENOMiHOG000233859.
HOVERGENiHBG008761.
InParanoidiP97504.
KOiK08896.
PhylomeDBiP97504.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR028840. BMX.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001562. Znf_Btk_motif.
[Graphical view]
PANTHERiPTHR24418:SF91. PTHR24418:SF91. 2 hits.
PfamiPF00779. BTK. 1 hit.
PF00169. PH. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00402. TECBTKDOMAIN.
PR00109. TYRKINASE.
SMARTiSM00107. BTK. 1 hit.
SM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97504-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESKSILEEL LLKKSQQKKK MSPNNYKERL FVLTKTSLSY YEYDKMKRGS
60 70 80 90 100
RKGSIEIKKI RCVEKVNLEE QTPVERQYPF QIVYKDGLLY VYASNEESRC
110 120 130 140 150
QWLKALQKEI RGNPHLLIKY HSGFFVDGKF LCCQQSCKAA PGCTLWEAYA
160 170 180 190 200
DLHIAISDEK HRAPTFPERL LKIPRAVPVL KMDASSSGAI LPQYDSYSKK
210 220 230 240 250
SCGSQPTSNI RYIPREDCPD WWQVRKLKSE EDIACSNQLE RNIASHSTSK
260 270 280 290 300
MSWGFPESSS SEEEENLHAY DWFAGNISRS QSEQLLRQKG KEGAFMVRNS
310 320 330 340 350
SQMGMYTVSL FSKAVNDKKG TVKHYHVHTN AENKLYLAEN YCFDSIPKLI
360 370 380 390 400
HYHQHNSAGM ITRLRHPVST KANKVPVSVA LGSGIWELKR EEITLLKELG
410 420 430 440 450
NGQFGVVQLG QWKGQYDVAV KMIKEGAMSE DEFFQEAQTM MKLSHPKLVK
460 470 480 490 500
FYGVCSKKYP IYIVTEYITN GCLLNYLKSH GKGLESCQLL EMCYDVCEGM
510 520 530 540 550
AFLESHQFIH RDLAARNCLV DSDLSVKVSD FGMTRYVLDD QYVSSVGTKF
560 570 580 590 600
PVKWSAPEVF HYFKYSSKSD VWAFGILMWE VFSLGKQPYD LYDNSEVVVK
610 620 630 640 650
VSQGHRLYRP QLASDTIYQI MYSCWHELPE KRPTFQQLLS AIEPLREQDK

P
Length:651
Mass (Da):75,001
Last modified:May 1, 1997 - v1
Checksum:i2E6B79B9491E569C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88091 mRNA. Translation: AAB47770.1.
AF012104 mRNA. Translation: AAC53370.1.
AK036707 mRNA. Translation: BAC29542.1.
AK040936 mRNA. Translation: BAC30753.1.
RefSeqiNP_033889.2. NM_009759.4.
UniGeneiMm.504.

Genome annotation databases

EnsembliENSMUST00000112263; ENSMUSP00000107882; ENSMUSG00000031377.
GeneIDi12169.
KEGGimmu:12169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88091 mRNA. Translation: AAB47770.1.
AF012104 mRNA. Translation: AAC53370.1.
AK036707 mRNA. Translation: BAC29542.1.
AK040936 mRNA. Translation: BAC30753.1.
RefSeqiNP_033889.2. NM_009759.4.
UniGeneiMm.504.

3D structure databases

ProteinModelPortaliP97504.
SMRiP97504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP97504. 1 interactor.
STRINGi10090.ENSMUSP00000107884.

Chemistry databases

BindingDBiP97504.
ChEMBLiCHEMBL2034793.

PTM databases

iPTMnetiP97504.
PhosphoSitePlusiP97504.

Proteomic databases

MaxQBiP97504.
PaxDbiP97504.
PRIDEiP97504.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112263; ENSMUSP00000107882; ENSMUSG00000031377.
GeneIDi12169.
KEGGimmu:12169.

Organism-specific databases

CTDi660.
MGIiMGI:1101778. Bmx.

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000119011.
HOGENOMiHOG000233859.
HOVERGENiHBG008761.
InParanoidiP97504.
KOiK08896.
PhylomeDBiP97504.

Enzyme and pathway databases

ReactomeiR-MMU-111465. Apoptotic cleavage of cellular proteins.
R-MMU-1660499. Synthesis of PIPs at the plasma membrane.

Miscellaneous databases

PROiP97504.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031377.
CleanExiMM_BMX.
ExpressionAtlasiP97504. baseline and differential.
GenevisibleiP97504. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR028840. BMX.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001562. Znf_Btk_motif.
[Graphical view]
PANTHERiPTHR24418:SF91. PTHR24418:SF91. 2 hits.
PfamiPF00779. BTK. 1 hit.
PF00169. PH. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00402. TECBTKDOMAIN.
PR00109. TYRKINASE.
SMARTiSM00107. BTK. 1 hit.
SM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBMX_MOUSE
AccessioniPrimary (citable) accession number: P97504
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.