Reviewed, UniProtKB/Swiss-Prot P97504 (BMX_MOUSE)

Names and origin

Protein names

Recommended name:
    Cytoplasmic tyrosine-protein kinase BMX
    EC=2.7.10.2
Alternative name(s):
    Bone marrow tyrosine kinase gene in chromosome X protein homolog

Gene names

Name:

Bmx

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Activity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Interacts with RUFY1 and RUFY2 By similarity.
Subcellular location	Cytoplasm Probable.
Tissue specificity	Specifically expressed in the endocardium of the developing heart as well as in the endocardium of the left ventricle and in the endothelium of large arteries in adult mice. Ref.2
Domain	SH2 domain mediates interaction with RUFY1 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. Contains 1 Btk-type zinc finger. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	SH2 domain Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Cytoplasmic tyrosine-protein kinase BMX		PRO_0000088064
Regions
	Domain	4 – 111	108	PH
	Domain	272 – 368	97	SH2
	Domain	393 – 646	254	Protein kinase
	Zinc finger	113 – 149	37	Btk-type
	Nucleotide binding	399 – 407	9	ATP By similarity
Sites
	Active site	512	1	Proton acceptor By similarity
	Metal binding	121	1	Zinc By similarity
	Metal binding	132	1	Zinc By similarity
	Metal binding	133	1	Zinc By similarity
	Metal binding	143	1	Zinc By similarity
	Binding site	421	1	ATP By similarity
Amino acid modifications
	Modified residue	542	1	Phosphotyrosine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97504-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 2E6B79B9491E569C	FASTA	651	75,001
	10 20 30 40 50 60 MESKSILEEL LLKKSQQKKK MSPNNYKERL FVLTKTSLSY YEYDKMKRGS RKGSIEIKKI 70 80 90 100 110 120 RCVEKVNLEE QTPVERQYPF QIVYKDGLLY VYASNEESRC QWLKALQKEI RGNPHLLIKY 130 140 150 160 170 180 HSGFFVDGKF LCCQQSCKAA PGCTLWEAYA DLHIAISDEK HRAPTFPERL LKIPRAVPVL 190 200 210 220 230 240 KMDASSSGAI LPQYDSYSKK SCGSQPTSNI RYIPREDCPD WWQVRKLKSE EDIACSNQLE 250 260 270 280 290 300 RNIASHSTSK MSWGFPESSS SEEEENLHAY DWFAGNISRS QSEQLLRQKG KEGAFMVRNS 310 320 330 340 350 360 SQMGMYTVSL FSKAVNDKKG TVKHYHVHTN AENKLYLAEN YCFDSIPKLI HYHQHNSAGM 370 380 390 400 410 420 ITRLRHPVST KANKVPVSVA LGSGIWELKR EEITLLKELG NGQFGVVQLG QWKGQYDVAV 430 440 450 460 470 480 KMIKEGAMSE DEFFQEAQTM MKLSHPKLVK FYGVCSKKYP IYIVTEYITN GCLLNYLKSH 490 500 510 520 530 540 GKGLESCQLL EMCYDVCEGM AFLESHQFIH RDLAARNCLV DSDLSVKVSD FGMTRYVLDD 550 560 570 580 590 600 QYVSSVGTKF PVKWSAPEVF HYFKYSSKSD VWAFGILMWE VFSLGKQPYD LYDNSEVVVK 610 620 630 640 650 VSQGHRLYRP QLASDTIYQI MYSCWHELPE KRPTFQQLLS AIEPLREQDK P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Predominant expression of murine Bmx in granulo-monocytic cells." Weil D., Power M.A., Catterral M., Li C.L. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Bone marrow.
[2]	"Bmx tyrosine kinase is specifically expressed in the endocardium and the endothelium of large arteries." Ekman N., Lymboussaki A., Vastrik I., Sarvas K., Kaipainen A., Alitalo K. Circulation 96:1729-1732(1997) [PubMed: 9323053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: 129/Sv.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Aorta and Bone.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U88091 mRNA. Translation: AAB47770.1. AF012104 mRNA. Translation: AAC53370.1. AK036707 mRNA. Translation: BAC29542.1. AK040936 mRNA. Translation: BAC30753.1.
IPI	IPI00126519.
RefSeq	NP_033889.2.
UniGene	Mm.504
3D structure databases
SMR	P97504. Positions 1-111, 3-147, 112-150, 261-369, 391-642.
ModBase	Search...
Protein-protein interaction databases
STRING	P97504.
PTM databases
PhosphoSite	P97504.
Proteomic databases
PRIDE	P97504.
Genome annotation databases
Ensembl	ENSMUST00000015547; ENSMUSP00000015547; ENSMUSG00000031377; Mus musculus. [Genome view] ENSMUST00000112263; ENSMUSP00000107882; ENSMUSG00000031377; Mus musculus. [Genome view] ENSMUST00000112265; ENSMUSP00000107884; ENSMUSG00000031377; Mus musculus. [Genome view]
GeneID	12169.
KEGG	mmu:12169.
UCSC	uc009uvj.1. mouse.
Organism-specific databases
CTD	12169.
MGI	MGI:1101778. Bmx.
Phylogenomic databases
eggNOG	roNOG05520.
HOVERGEN	P97504.
InParanoid	P97504.
PhylomeDB	P97504.
Enzyme and pathway databases
BRENDA	2.7.10.2. 244.
Gene expression databases
ArrayExpress	P97504.
Bgee	P97504.
CleanEx	MM_BMX.
Genevestigator	P97504.
GermOnline	ENSMUSG00000031377. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR001562. Znf_Btk_motif. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.30.505.10. SH2. 1 hit.
PANTHER	PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF00779. BTK. 1 hit. PF00169. PH. 1 hit. PF00017. SH2. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00402. TECBTKDOMAIN.
SMART	SM00107. BTK. 1 hit. SM00233. PH. 1 hit. SM00252. SH2. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS51113. ZF_BTK. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	280543.
SOURCE	Search...

Entry information

Entry name

BMX_MOUSE

Accession

Primary (citable) accession number: P97504

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	May 1, 1997
Last modified:	February 9, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)