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P97504

- BMX_MOUSE

UniProt

P97504 - BMX_MOUSE

Protein

Cytoplasmic tyrosine-protein kinase BMX

Gene

Bmx

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.1 Publication

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Enzyme regulationi

    TEK and vascular endothelial growth factor receptor 1 (FLT1) stimulate BMX tyrosine kinase activity. Activated by integrins through the mediation of PTK2/FAK1 By similarity. Activated by TNF through the mediation of TNFRSF1B By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi121 – 1211ZincBy similarity
    Metal bindingi132 – 1321ZincBy similarity
    Metal bindingi133 – 1331ZincBy similarity
    Metal bindingi143 – 1431ZincBy similarity
    Binding sitei421 – 4211ATPPROSITE-ProRule annotation
    Active sitei512 – 5121Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri113 – 14937Btk-typePROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi399 – 4079ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. cell adhesion Source: UniProtKB-KW
    3. intracellular signal transduction Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Apoptosis, Cell adhesion

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_224460. Apoptotic cleavage of cellular proteins.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytoplasmic tyrosine-protein kinase BMX (EC:2.7.10.2)
    Alternative name(s):
    Bone marrow tyrosine kinase gene in chromosome X protein homolog
    Gene namesi
    Name:Bmx
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1101778. Bmx.

    Subcellular locationi

    Cytoplasm By similarity
    Note: Localizes to the edges of spreading cells when complexed with BCAR1.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 651651Cytoplasmic tyrosine-protein kinase BMXPRO_0000088064Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei542 – 5421Phosphotyrosine; by SRC and autocatalysisBy similarity

    Post-translational modificationi

    Phosphorylated in response to protein I/II and to LPS. Phosphorylation at Tyr-542 by SRC and by autocatalysis leads to activation and is required for STAT3 phosphorylation by BMX.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP97504.

    PTM databases

    PhosphoSiteiP97504.

    Expressioni

    Tissue specificityi

    Specifically expressed in the endocardium of the developing heart as well as in the endocardium of the left ventricle and in the endothelium of large arteries in adult mice.1 Publication

    Gene expression databases

    ArrayExpressiP97504.
    BgeeiP97504.
    CleanExiMM_BMX.
    GenevestigatoriP97504.

    Interactioni

    Subunit structurei

    Interacts with BCAR1, CAV1, MYD88, PTK2/FAK1, RUFY1, RUFY2, STAT3, TIRAP and TNFRSF1B.By similarity

    Protein-protein interaction databases

    IntActiP97504. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP97504.
    SMRiP97504. Positions 6-150, 219-649.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 111108PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini272 – 36897SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini393 – 646254Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Domaini

    SH2 domain mediates interaction with RUFY1.By similarity

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.PROSITE-ProRule annotation
    Contains 1 Btk-type zinc finger.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri113 – 14937Btk-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    SH2 domain, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00640000091251.
    HOGENOMiHOG000233859.
    HOVERGENiHBG008761.
    InParanoidiP97504.
    KOiK08896.
    PhylomeDBiP97504.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    3.30.505.10. 1 hit.
    InterProiIPR028840. BMX.
    IPR011009. Kinase-like_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR001562. Znf_Btk_motif.
    [Graphical view]
    PANTHERiPTHR24418:SF91. PTHR24418:SF91. 1 hit.
    PfamiPF00779. BTK. 1 hit.
    PF00169. PH. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00402. TECBTKDOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00107. BTK. 1 hit.
    SM00233. PH. 1 hit.
    SM00252. SH2. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS51113. ZF_BTK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P97504-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESKSILEEL LLKKSQQKKK MSPNNYKERL FVLTKTSLSY YEYDKMKRGS    50
    RKGSIEIKKI RCVEKVNLEE QTPVERQYPF QIVYKDGLLY VYASNEESRC 100
    QWLKALQKEI RGNPHLLIKY HSGFFVDGKF LCCQQSCKAA PGCTLWEAYA 150
    DLHIAISDEK HRAPTFPERL LKIPRAVPVL KMDASSSGAI LPQYDSYSKK 200
    SCGSQPTSNI RYIPREDCPD WWQVRKLKSE EDIACSNQLE RNIASHSTSK 250
    MSWGFPESSS SEEEENLHAY DWFAGNISRS QSEQLLRQKG KEGAFMVRNS 300
    SQMGMYTVSL FSKAVNDKKG TVKHYHVHTN AENKLYLAEN YCFDSIPKLI 350
    HYHQHNSAGM ITRLRHPVST KANKVPVSVA LGSGIWELKR EEITLLKELG 400
    NGQFGVVQLG QWKGQYDVAV KMIKEGAMSE DEFFQEAQTM MKLSHPKLVK 450
    FYGVCSKKYP IYIVTEYITN GCLLNYLKSH GKGLESCQLL EMCYDVCEGM 500
    AFLESHQFIH RDLAARNCLV DSDLSVKVSD FGMTRYVLDD QYVSSVGTKF 550
    PVKWSAPEVF HYFKYSSKSD VWAFGILMWE VFSLGKQPYD LYDNSEVVVK 600
    VSQGHRLYRP QLASDTIYQI MYSCWHELPE KRPTFQQLLS AIEPLREQDK 650
    P 651
    Length:651
    Mass (Da):75,001
    Last modified:May 1, 1997 - v1
    Checksum:i2E6B79B9491E569C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U88091 mRNA. Translation: AAB47770.1.
    AF012104 mRNA. Translation: AAC53370.1.
    AK036707 mRNA. Translation: BAC29542.1.
    AK040936 mRNA. Translation: BAC30753.1.
    RefSeqiNP_033889.2. NM_009759.4.
    UniGeneiMm.504.

    Genome annotation databases

    EnsembliENSMUST00000112263; ENSMUSP00000107882; ENSMUSG00000031377.
    GeneIDi12169.
    KEGGimmu:12169.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U88091 mRNA. Translation: AAB47770.1 .
    AF012104 mRNA. Translation: AAC53370.1 .
    AK036707 mRNA. Translation: BAC29542.1 .
    AK040936 mRNA. Translation: BAC30753.1 .
    RefSeqi NP_033889.2. NM_009759.4.
    UniGenei Mm.504.

    3D structure databases

    ProteinModelPortali P97504.
    SMRi P97504. Positions 6-150, 219-649.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P97504. 1 interaction.

    Chemistry

    ChEMBLi CHEMBL2034793.

    PTM databases

    PhosphoSitei P97504.

    Proteomic databases

    PRIDEi P97504.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000112263 ; ENSMUSP00000107882 ; ENSMUSG00000031377 .
    GeneIDi 12169.
    KEGGi mmu:12169.

    Organism-specific databases

    CTDi 660.
    MGIi MGI:1101778. Bmx.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00640000091251.
    HOGENOMi HOG000233859.
    HOVERGENi HBG008761.
    InParanoidi P97504.
    KOi K08896.
    PhylomeDBi P97504.

    Enzyme and pathway databases

    Reactomei REACT_224460. Apoptotic cleavage of cellular proteins.

    Miscellaneous databases

    NextBioi 280543.
    PROi P97504.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97504.
    Bgeei P97504.
    CleanExi MM_BMX.
    Genevestigatori P97504.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    3.30.505.10. 1 hit.
    InterProi IPR028840. BMX.
    IPR011009. Kinase-like_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR001562. Znf_Btk_motif.
    [Graphical view ]
    PANTHERi PTHR24418:SF91. PTHR24418:SF91. 1 hit.
    Pfami PF00779. BTK. 1 hit.
    PF00169. PH. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00402. TECBTKDOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00107. BTK. 1 hit.
    SM00233. PH. 1 hit.
    SM00252. SH2. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS51113. ZF_BTK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Predominant expression of murine Bmx in granulo-monocytic cells."
      Weil D., Power M.A., Catterral M., Li C.L.
      Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Bone marrow.
    2. "Bmx tyrosine kinase is specifically expressed in the endocardium and the endothelium of large arteries."
      Ekman N., Lymboussaki A., Vastrik I., Sarvas K., Kaipainen A., Alitalo K.
      Circulation 96:1729-1732(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: 129/Sv.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Aorta and Bone.
    4. "Bmx tyrosine kinase has a redundant function downstream of angiopoietin and vascular endothelial growth factor receptors in arterial endothelium."
      Rajantie I., Ekman N., Iljin K., Arighi E., Gunji Y., Kaukonen J., Palotie A., Dewerchin M., Carmeliet P., Alitalo K.
      Mol. Cell. Biol. 21:4647-4655(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION, FUNCTION.
    5. "Signaling network of the Btk family kinases."
      Qiu Y., Kung H.J.
      Oncogene 19:5651-5661(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON FUNCTION.
    6. "The Tec family of cytoplasmic tyrosine kinases: mammalian Btk, Bmx, Itk, Tec, Txk and homologs in other species."
      Smith C.I., Islam T.C., Mattsson P.T., Mohamed A.J., Nore B.F., Vihinen M.
      Bioessays 23:436-446(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON FUNCTION.
    7. Cited for: REVIEW ON FUNCTION.

    Entry informationi

    Entry nameiBMX_MOUSE
    AccessioniPrimary (citable) accession number: P97504
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3