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P97504

- BMX_MOUSE

UniProt

P97504 - BMX_MOUSE

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Protein
Cytoplasmic tyrosine-protein kinase BMX
Gene
Bmx
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Enzyme regulationi

TEK and vascular endothelial growth factor receptor 1 (FLT1) stimulate BMX tyrosine kinase activity. Activated by integrins through the mediation of PTK2/FAK1 By similarity. Activated by TNF through the mediation of TNFRSF1B By similarity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi121 – 1211Zinc By similarity
Metal bindingi132 – 1321Zinc By similarity
Metal bindingi133 – 1331Zinc By similarity
Metal bindingi143 – 1431Zinc By similarity
Binding sitei421 – 4211ATP By similarity
Active sitei512 – 5121Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri113 – 14937Btk-type
Add
BLAST
Nucleotide bindingi399 – 4079ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. cell adhesion Source: UniProtKB-KW
  3. intracellular signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Apoptosis, Cell adhesion

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_224460. Apoptotic cleavage of cellular proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic tyrosine-protein kinase BMX (EC:2.7.10.2)
Alternative name(s):
Bone marrow tyrosine kinase gene in chromosome X protein homolog
Gene namesi
Name:Bmx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1101778. Bmx.

Subcellular locationi

Cytoplasm By similarity
Note: Localizes to the edges of spreading cells when complexed with BCAR1 By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 651651Cytoplasmic tyrosine-protein kinase BMX
PRO_0000088064Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei542 – 5421Phosphotyrosine; by SRC and autocatalysis By similarity

Post-translational modificationi

Phosphorylated in response to protein I/II and to LPS By similarity. Phosphorylation at Tyr-542 by SRC and by autocatalysis leads to activation and is required for STAT3 phosphorylation by BMX By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP97504.

PTM databases

PhosphoSiteiP97504.

Expressioni

Tissue specificityi

Specifically expressed in the endocardium of the developing heart as well as in the endocardium of the left ventricle and in the endothelium of large arteries in adult mice.1 Publication

Gene expression databases

ArrayExpressiP97504.
BgeeiP97504.
CleanExiMM_BMX.
GenevestigatoriP97504.

Interactioni

Subunit structurei

Interacts with BCAR1, CAV1, MYD88, PTK2/FAK1, RUFY1, RUFY2, STAT3, TIRAP and TNFRSF1B By similarity.

Protein-protein interaction databases

IntActiP97504. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP97504.
SMRiP97504. Positions 6-150, 219-649.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 111108PH
Add
BLAST
Domaini272 – 36897SH2
Add
BLAST
Domaini393 – 646254Protein kinase
Add
BLAST

Domaini

SH2 domain mediates interaction with RUFY1 By similarity.

Sequence similaritiesi

Contains 1 PH domain.
Contains 1 SH2 domain.

Keywords - Domaini

SH2 domain, Zinc-finger

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00640000091251.
HOGENOMiHOG000233859.
HOVERGENiHBG008761.
InParanoidiP97504.
KOiK08896.
PhylomeDBiP97504.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR028840. BMX.
IPR011009. Kinase-like_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001562. Znf_Btk_motif.
[Graphical view]
PANTHERiPTHR24418:SF91. PTHR24418:SF91. 1 hit.
PfamiPF00779. BTK. 1 hit.
PF00169. PH. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00402. TECBTKDOMAIN.
PR00109. TYRKINASE.
SMARTiSM00107. BTK. 1 hit.
SM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97504-1 [UniParc]FASTAAdd to Basket

« Hide

MESKSILEEL LLKKSQQKKK MSPNNYKERL FVLTKTSLSY YEYDKMKRGS    50
RKGSIEIKKI RCVEKVNLEE QTPVERQYPF QIVYKDGLLY VYASNEESRC 100
QWLKALQKEI RGNPHLLIKY HSGFFVDGKF LCCQQSCKAA PGCTLWEAYA 150
DLHIAISDEK HRAPTFPERL LKIPRAVPVL KMDASSSGAI LPQYDSYSKK 200
SCGSQPTSNI RYIPREDCPD WWQVRKLKSE EDIACSNQLE RNIASHSTSK 250
MSWGFPESSS SEEEENLHAY DWFAGNISRS QSEQLLRQKG KEGAFMVRNS 300
SQMGMYTVSL FSKAVNDKKG TVKHYHVHTN AENKLYLAEN YCFDSIPKLI 350
HYHQHNSAGM ITRLRHPVST KANKVPVSVA LGSGIWELKR EEITLLKELG 400
NGQFGVVQLG QWKGQYDVAV KMIKEGAMSE DEFFQEAQTM MKLSHPKLVK 450
FYGVCSKKYP IYIVTEYITN GCLLNYLKSH GKGLESCQLL EMCYDVCEGM 500
AFLESHQFIH RDLAARNCLV DSDLSVKVSD FGMTRYVLDD QYVSSVGTKF 550
PVKWSAPEVF HYFKYSSKSD VWAFGILMWE VFSLGKQPYD LYDNSEVVVK 600
VSQGHRLYRP QLASDTIYQI MYSCWHELPE KRPTFQQLLS AIEPLREQDK 650
P 651
Length:651
Mass (Da):75,001
Last modified:May 1, 1997 - v1
Checksum:i2E6B79B9491E569C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U88091 mRNA. Translation: AAB47770.1.
AF012104 mRNA. Translation: AAC53370.1.
AK036707 mRNA. Translation: BAC29542.1.
AK040936 mRNA. Translation: BAC30753.1.
RefSeqiNP_033889.2. NM_009759.4.
UniGeneiMm.504.

Genome annotation databases

EnsembliENSMUST00000112263; ENSMUSP00000107882; ENSMUSG00000031377.
GeneIDi12169.
KEGGimmu:12169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U88091 mRNA. Translation: AAB47770.1 .
AF012104 mRNA. Translation: AAC53370.1 .
AK036707 mRNA. Translation: BAC29542.1 .
AK040936 mRNA. Translation: BAC30753.1 .
RefSeqi NP_033889.2. NM_009759.4.
UniGenei Mm.504.

3D structure databases

ProteinModelPortali P97504.
SMRi P97504. Positions 6-150, 219-649.
ModBasei Search...

Protein-protein interaction databases

IntActi P97504. 1 interaction.

Chemistry

ChEMBLi CHEMBL2034793.

PTM databases

PhosphoSitei P97504.

Proteomic databases

PRIDEi P97504.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000112263 ; ENSMUSP00000107882 ; ENSMUSG00000031377 .
GeneIDi 12169.
KEGGi mmu:12169.

Organism-specific databases

CTDi 660.
MGIi MGI:1101778. Bmx.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00640000091251.
HOGENOMi HOG000233859.
HOVERGENi HBG008761.
InParanoidi P97504.
KOi K08896.
PhylomeDBi P97504.

Enzyme and pathway databases

Reactomei REACT_224460. Apoptotic cleavage of cellular proteins.

Miscellaneous databases

NextBioi 280543.
PROi P97504.
SOURCEi Search...

Gene expression databases

ArrayExpressi P97504.
Bgeei P97504.
CleanExi MM_BMX.
Genevestigatori P97504.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProi IPR028840. BMX.
IPR011009. Kinase-like_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001562. Znf_Btk_motif.
[Graphical view ]
PANTHERi PTHR24418:SF91. PTHR24418:SF91. 1 hit.
Pfami PF00779. BTK. 1 hit.
PF00169. PH. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00402. TECBTKDOMAIN.
PR00109. TYRKINASE.
SMARTi SM00107. BTK. 1 hit.
SM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Predominant expression of murine Bmx in granulo-monocytic cells."
    Weil D., Power M.A., Catterral M., Li C.L.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Bone marrow.
  2. "Bmx tyrosine kinase is specifically expressed in the endocardium and the endothelium of large arteries."
    Ekman N., Lymboussaki A., Vastrik I., Sarvas K., Kaipainen A., Alitalo K.
    Circulation 96:1729-1732(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: 129/Sv.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Aorta and Bone.
  4. "Bmx tyrosine kinase has a redundant function downstream of angiopoietin and vascular endothelial growth factor receptors in arterial endothelium."
    Rajantie I., Ekman N., Iljin K., Arighi E., Gunji Y., Kaukonen J., Palotie A., Dewerchin M., Carmeliet P., Alitalo K.
    Mol. Cell. Biol. 21:4647-4655(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, FUNCTION.
  5. "Signaling network of the Btk family kinases."
    Qiu Y., Kung H.J.
    Oncogene 19:5651-5661(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON FUNCTION.
  6. "The Tec family of cytoplasmic tyrosine kinases: mammalian Btk, Bmx, Itk, Tec, Txk and homologs in other species."
    Smith C.I., Islam T.C., Mattsson P.T., Mohamed A.J., Nore B.F., Vihinen M.
    Bioessays 23:436-446(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON FUNCTION.
  7. Cited for: REVIEW ON FUNCTION.

Entry informationi

Entry nameiBMX_MOUSE
AccessioniPrimary (citable) accession number: P97504
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: May 1, 1997
Last modified: September 3, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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