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P97504 (BMX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytoplasmic tyrosine-protein kinase BMX

EC=2.7.10.2
Alternative name(s):
Bone marrow tyrosine kinase gene in chromosome X protein homolog
Gene names
Name:Bmx
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length651 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells. Ref.4

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Enzyme regulation

TEK and vascular endothelial growth factor receptor 1 (FLT1) stimulate BMX tyrosine kinase activity. Activated by integrins through the mediation of PTK2/FAK1 By similarity. Activated by TNF through the mediation of TNFRSF1B By similarity. Ref.4

Subunit structure

Interacts with BCAR1, CAV1, MYD88, PTK2/FAK1, RUFY1, RUFY2, STAT3, TIRAP and TNFRSF1B By similarity.

Subcellular location

Cytoplasm By similarity. Note: Localizes to the edges of spreading cells when complexed with BCAR1 By similarity.

Tissue specificity

Specifically expressed in the endocardium of the developing heart as well as in the endocardium of the left ventricle and in the endothelium of large arteries in adult mice. Ref.2

Domain

SH2 domain mediates interaction with RUFY1 By similarity.

Post-translational modification

Phosphorylated in response to protein I/II and to LPS By similarity. Phosphorylation at Tyr-542 by SRC and by autocatalysis leads to activation and is required for STAT3 phosphorylation by BMX By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.

Contains 1 Btk-type zinc finger.

Contains 1 PH domain.

Contains 1 protein kinase domain.

Contains 1 SH2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 651651Cytoplasmic tyrosine-protein kinase BMX
PRO_0000088064

Regions

Domain4 – 111108PH
Domain272 – 36897SH2
Domain393 – 646254Protein kinase
Zinc finger113 – 14937Btk-type
Nucleotide binding399 – 4079ATP By similarity

Sites

Active site5121Proton acceptor By similarity
Metal binding1211Zinc By similarity
Metal binding1321Zinc By similarity
Metal binding1331Zinc By similarity
Metal binding1431Zinc By similarity
Binding site4211ATP By similarity

Amino acid modifications

Modified residue5421Phosphotyrosine; by SRC and autocatalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
P97504 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 2E6B79B9491E569C

FASTA65175,001
        10         20         30         40         50         60 
MESKSILEEL LLKKSQQKKK MSPNNYKERL FVLTKTSLSY YEYDKMKRGS RKGSIEIKKI 

        70         80         90        100        110        120 
RCVEKVNLEE QTPVERQYPF QIVYKDGLLY VYASNEESRC QWLKALQKEI RGNPHLLIKY 

       130        140        150        160        170        180 
HSGFFVDGKF LCCQQSCKAA PGCTLWEAYA DLHIAISDEK HRAPTFPERL LKIPRAVPVL 

       190        200        210        220        230        240 
KMDASSSGAI LPQYDSYSKK SCGSQPTSNI RYIPREDCPD WWQVRKLKSE EDIACSNQLE 

       250        260        270        280        290        300 
RNIASHSTSK MSWGFPESSS SEEEENLHAY DWFAGNISRS QSEQLLRQKG KEGAFMVRNS 

       310        320        330        340        350        360 
SQMGMYTVSL FSKAVNDKKG TVKHYHVHTN AENKLYLAEN YCFDSIPKLI HYHQHNSAGM 

       370        380        390        400        410        420 
ITRLRHPVST KANKVPVSVA LGSGIWELKR EEITLLKELG NGQFGVVQLG QWKGQYDVAV 

       430        440        450        460        470        480 
KMIKEGAMSE DEFFQEAQTM MKLSHPKLVK FYGVCSKKYP IYIVTEYITN GCLLNYLKSH 

       490        500        510        520        530        540 
GKGLESCQLL EMCYDVCEGM AFLESHQFIH RDLAARNCLV DSDLSVKVSD FGMTRYVLDD 

       550        560        570        580        590        600 
QYVSSVGTKF PVKWSAPEVF HYFKYSSKSD VWAFGILMWE VFSLGKQPYD LYDNSEVVVK 

       610        620        630        640        650 
VSQGHRLYRP QLASDTIYQI MYSCWHELPE KRPTFQQLLS AIEPLREQDK P 

« Hide

References

« Hide 'large scale' references
[1]"Predominant expression of murine Bmx in granulo-monocytic cells."
Weil D., Power M.A., Catterral M., Li C.L.
Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Bone marrow.
[2]"Bmx tyrosine kinase is specifically expressed in the endocardium and the endothelium of large arteries."
Ekman N., Lymboussaki A., Vastrik I., Sarvas K., Kaipainen A., Alitalo K.
Circulation 96:1729-1732(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: 129/Sv.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Aorta and Bone.
[4]"Bmx tyrosine kinase has a redundant function downstream of angiopoietin and vascular endothelial growth factor receptors in arterial endothelium."
Rajantie I., Ekman N., Iljin K., Arighi E., Gunji Y., Kaukonen J., Palotie A., Dewerchin M., Carmeliet P., Alitalo K.
Mol. Cell. Biol. 21:4647-4655(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION, FUNCTION.
[5]"Signaling network of the Btk family kinases."
Qiu Y., Kung H.J.
Oncogene 19:5651-5661(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON FUNCTION.
[6]"The Tec family of cytoplasmic tyrosine kinases: mammalian Btk, Bmx, Itk, Tec, Txk and homologs in other species."
Smith C.I., Islam T.C., Mattsson P.T., Mohamed A.J., Nore B.F., Vihinen M.
Bioessays 23:436-446(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON FUNCTION.
[7]"Tec kinases, actin, and cell adhesion."
Gomez-Rodriguez J., Readinger J.A., Viorritto I.C., Mueller K.L., Houghtling R.A., Schwartzberg P.L.
Immunol. Rev. 218:45-64(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U88091 mRNA. Translation: AAB47770.1.
AF012104 mRNA. Translation: AAC53370.1.
AK036707 mRNA. Translation: BAC29542.1.
AK040936 mRNA. Translation: BAC30753.1.
RefSeqNP_033889.2. NM_009759.4.
UniGeneMm.504.

3D structure databases

ProteinModelPortalP97504.
SMRP97504. Positions 6-150, 188-651.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP97504. 1 interaction.

Chemistry

ChEMBLCHEMBL2034793.

PTM databases

PhosphoSiteP97504.

Proteomic databases

PRIDEP97504.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000112263; ENSMUSP00000107882; ENSMUSG00000031377.
GeneID12169.
KEGGmmu:12169.

Organism-specific databases

CTD660.
MGIMGI:1101778. Bmx.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00640000091251.
HOGENOMHOG000233859.
HOVERGENHBG008761.
InParanoidP97504.
KOK08896.
PhylomeDBP97504.

Gene expression databases

ArrayExpressP97504.
BgeeP97504.
CleanExMM_BMX.
GenevestigatorP97504.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProIPR028840. BMX.
IPR011009. Kinase-like_dom.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001562. Znf_Btk_motif.
[Graphical view]
PANTHERPTHR24418:SF91. PTHR24418:SF91. 1 hit.
PfamPF00779. BTK. 1 hit.
PF00169. PH. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSPR00401. SH2DOMAIN.
PR00402. TECBTKDOMAIN.
PR00109. TYRKINASE.
SMARTSM00107. BTK. 1 hit.
SM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio280543.
PROP97504.
SOURCESearch...

Entry information

Entry nameBMX_MOUSE
AccessionPrimary (citable) accession number: P97504
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot