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Protein

Homeobox protein Nkx-3.2

Gene

Nkx3-2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor that acts as a negative regulator of chondrocyte maturation. PLays a role in distal stomach development; required for proper antral-pyloric morphogenesis and development of antral-type epithelium. In concert with GSC, defines the structural components of the middle ear; required for tympanic ring and gonium development and in the regulation of the width of the malleus.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi206 – 26560HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • animal organ development Source: MGI
  • determination of left/right symmetry Source: MGI
  • digestive system development Source: MGI
  • embryonic skeletal system development Source: MGI
  • intestinal epithelial cell development Source: MGI
  • middle ear morphogenesis Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of chondrocyte differentiation Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • organ formation Source: MGI
  • pancreas development Source: MGI
  • skeletal system development Source: MGI
  • skeletal system morphogenesis Source: MGI
  • spleen development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Nkx-3.2
Alternative name(s):
Bagpipe homeobox protein homolog 1
Homeobox protein NK-3 homolog B
Gene namesi
Name:Nkx3-2
Synonyms:Bapx1, Nkx-3.2, Nkx3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:108015. Nkx3-2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Homeobox protein Nkx-3.2PRO_0000048948Add
BLAST

Proteomic databases

EPDiP97503.
MaxQBiP97503.
PaxDbiP97503.
PeptideAtlasiP97503.
PRIDEiP97503.

PTM databases

iPTMnetiP97503.
PhosphoSiteiP97503.

Expressioni

Tissue specificityi

Expressed widely in mesoderm at the gastroduodenal junction (at protein level). Expressed in visceral mesoderm and embryonic skeleton. Expression is restricted to immature proliferative chondrocytes during endochondral ossification.2 Publications

Developmental stagei

During embryogenesis, expressed in a discrete domain within the mandibular component of the first branchial arch and later in the primordia of middle earassociated bones, the gonium and tympanic ring.1 Publication

Gene expression databases

BgeeiP97503.
CleanExiMM_NKX3-2.
GenevisibleiP97503. MM.

Interactioni

Protein-protein interaction databases

BioGridi198300. 3 interactions.
STRINGi10090.ENSMUSP00000051990.

Structurei

3D structure databases

ProteinModelPortaliP97503.
SMRiP97503. Positions 217-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi194 – 1985Poly-Glu
Compositional biasi327 – 3304Poly-Ala

Sequence similaritiesi

Belongs to the NK-3 homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0842. Eukaryota.
ENOG410XR21. LUCA.
GeneTreeiENSGT00840000129671.
HOGENOMiHOG000231923.
HOVERGENiHBG006689.
InParanoidiP97503.
KOiK09995.
OMAiESPGGWD.
OrthoDBiEOG7QZG9Z.
TreeFamiTF351204.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRGSGTLT PFSIQAILNK KEERGGLATP EGRPAPGGTE VAVTAAPAVC
60 70 80 90 100
CWRIFGETEA GALGGAEDSL LASPARTRTA VGQSAESPGG WDSDSALSEE
110 120 130 140 150
NEGRRRCADV PGASGTGRAR VTLGLDQPGC ELHAAKDLEE EAPVRSDSEM
160 170 180 190 200
SASVSGDHSP RGEDDSVSPG GARVPGLRGA AGSGASGGQA GGVEEEEEPA
210 220 230 240 250
APKPRKKRSR AAFSHAQVFE LERRFNHQRY LSGPERADLA ASLKLTETQV
260 270 280 290 300
KIWFQNRRYK TKRRQMAADL LASAPAAKKV AVKVLVRDDQ RQYLPGEVLR
310 320 330
PPSLLPLQPS YYYPYYCLPG WALSTCAAAA GTQ
Length:333
Mass (Da):35,167
Last modified:July 27, 2011 - v2
Checksum:iD089523714C86BA6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti134 – 1341A → P in AAB47764 (PubMed:9256352).Curated
Sequence conflicti168 – 1681S → T in AAB47764 (PubMed:9256352).Curated
Sequence conflicti292 – 2921Q → L in AAB47764 (PubMed:9256352).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87957 mRNA. Translation: AAB47764.1.
CH466524 Genomic DNA. Translation: EDL37578.1.
BC145872 mRNA. Translation: AAI45873.1.
BC145874 mRNA. Translation: AAI45875.1.
CCDSiCCDS19259.1.
RefSeqiNP_031550.2. NM_007524.3.
UniGeneiMm.2530.

Genome annotation databases

EnsembliENSMUST00000060820; ENSMUSP00000051990; ENSMUSG00000049691.
GeneIDi12020.
KEGGimmu:12020.
UCSCiuc008xhc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87957 mRNA. Translation: AAB47764.1.
CH466524 Genomic DNA. Translation: EDL37578.1.
BC145872 mRNA. Translation: AAI45873.1.
BC145874 mRNA. Translation: AAI45875.1.
CCDSiCCDS19259.1.
RefSeqiNP_031550.2. NM_007524.3.
UniGeneiMm.2530.

3D structure databases

ProteinModelPortaliP97503.
SMRiP97503. Positions 217-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198300. 3 interactions.
STRINGi10090.ENSMUSP00000051990.

PTM databases

iPTMnetiP97503.
PhosphoSiteiP97503.

Proteomic databases

EPDiP97503.
MaxQBiP97503.
PaxDbiP97503.
PeptideAtlasiP97503.
PRIDEiP97503.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060820; ENSMUSP00000051990; ENSMUSG00000049691.
GeneIDi12020.
KEGGimmu:12020.
UCSCiuc008xhc.1. mouse.

Organism-specific databases

CTDi579.
MGIiMGI:108015. Nkx3-2.

Phylogenomic databases

eggNOGiKOG0842. Eukaryota.
ENOG410XR21. LUCA.
GeneTreeiENSGT00840000129671.
HOGENOMiHOG000231923.
HOVERGENiHBG006689.
InParanoidiP97503.
KOiK09995.
OMAiESPGGWD.
OrthoDBiEOG7QZG9Z.
TreeFamiTF351204.

Miscellaneous databases

PROiP97503.
SOURCEiSearch...

Gene expression databases

BgeeiP97503.
CleanExiMM_NKX3-2.
GenevisibleiP97503. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Bapx1: an evolutionary conserved homologue of the Drosophila bagpipe homeobox gene is expressed in splanchnic mesoderm and the embryonic skeleton."
    Tribioli C., Frasch M., Lufkin T.
    Mech. Dev. 65:145-162(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Bapx1 regulates patterning in the middle ear: altered regulatory role in the transition from the proximal jaw during vertebrate evolution."
    Tucker A.S., Watson R.P., Lettice L.A., Yamada G., Hill R.E.
    Development 131:1235-1245(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  5. "Nkx3.2/Bapx1 acts as a negative regulator of chondrocyte maturation."
    Provot S., Kempf H., Murtaugh L.C., Chung U.I., Kim D.W., Chyung J., Kronenberg H.M., Lassar A.B.
    Development 133:651-662(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. "Role of the homeodomain transcription factor Bapx1 in mouse distal stomach development."
    Verzi M.P., Stanfel M.N., Moses K.A., Kim B.M., Zhang Y., Schwartz R.J., Shivdasani R.A., Zimmer W.E.
    Gastroenterology 136:1701-1710(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNKX32_MOUSE
AccessioniPrimary (citable) accession number: P97503
Secondary accession number(s): A6H6G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.