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Protein

Homeobox protein Nkx-3.2

Gene

Nkx3-2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor that acts as a negative regulator of chondrocyte maturation. PLays a role in distal stomach development; required for proper antral-pyloric morphogenesis and development of antral-type epithelium. In concert with GSC, defines the structural components of the middle ear; required for tympanic ring and gonium development and in the regulation of the width of the malleus.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi206 – 265HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

  • animal organ development Source: MGI
  • animal organ formation Source: MGI
  • determination of left/right symmetry Source: MGI
  • digestive system development Source: MGI
  • embryonic skeletal system development Source: MGI
  • intestinal epithelial cell development Source: MGI
  • middle ear morphogenesis Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of chondrocyte differentiation Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: NTNU_SB
  • pancreas development Source: MGI
  • skeletal system development Source: MGI
  • skeletal system morphogenesis Source: MGI
  • spleen development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Nkx-3.2
Alternative name(s):
Bagpipe homeobox protein homolog 1
Homeobox protein NK-3 homolog B
Gene namesi
Name:Nkx3-2
Synonyms:Bapx1, Nkx-3.2, Nkx3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:108015 Nkx3-2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000489481 – 333Homeobox protein Nkx-3.2Add BLAST333

Proteomic databases

EPDiP97503
MaxQBiP97503
PaxDbiP97503
PeptideAtlasiP97503
PRIDEiP97503

PTM databases

iPTMnetiP97503
PhosphoSitePlusiP97503

Expressioni

Tissue specificityi

Expressed widely in mesoderm at the gastroduodenal junction (at protein level). Expressed in visceral mesoderm and embryonic skeleton. Expression is restricted to immature proliferative chondrocytes during endochondral ossification.2 Publications

Developmental stagei

During embryogenesis, expressed in a discrete domain within the mandibular component of the first branchial arch and later in the primordia of middle earassociated bones, the gonium and tympanic ring.1 Publication

Gene expression databases

BgeeiENSMUSG00000049691
CleanExiMM_NKX3-2
GenevisibleiP97503 MM

Interactioni

Protein-protein interaction databases

BioGridi198300, 3 interactors
CORUMiP97503
STRINGi10090.ENSMUSP00000051990

Structurei

3D structure databases

ProteinModelPortaliP97503
SMRiP97503
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi194 – 198Poly-Glu5
Compositional biasi327 – 330Poly-Ala4

Sequence similaritiesi

Belongs to the NK-3 homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0842 Eukaryota
ENOG410XR21 LUCA
GeneTreeiENSGT00910000144003
HOGENOMiHOG000231923
HOVERGENiHBG006689
InParanoidiP97503
KOiK09995
OMAiESPGGWD
OrthoDBiEOG091G0V1X
TreeFamiTF351204

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR020479 Homeobox_metazoa
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PRINTSiPR00024 HOMEOBOX
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

P97503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRGSGTLT PFSIQAILNK KEERGGLATP EGRPAPGGTE VAVTAAPAVC
60 70 80 90 100
CWRIFGETEA GALGGAEDSL LASPARTRTA VGQSAESPGG WDSDSALSEE
110 120 130 140 150
NEGRRRCADV PGASGTGRAR VTLGLDQPGC ELHAAKDLEE EAPVRSDSEM
160 170 180 190 200
SASVSGDHSP RGEDDSVSPG GARVPGLRGA AGSGASGGQA GGVEEEEEPA
210 220 230 240 250
APKPRKKRSR AAFSHAQVFE LERRFNHQRY LSGPERADLA ASLKLTETQV
260 270 280 290 300
KIWFQNRRYK TKRRQMAADL LASAPAAKKV AVKVLVRDDQ RQYLPGEVLR
310 320 330
PPSLLPLQPS YYYPYYCLPG WALSTCAAAA GTQ
Length:333
Mass (Da):35,167
Last modified:July 27, 2011 - v2
Checksum:iD089523714C86BA6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134A → P in AAB47764 (PubMed:9256352).Curated1
Sequence conflicti168S → T in AAB47764 (PubMed:9256352).Curated1
Sequence conflicti292Q → L in AAB47764 (PubMed:9256352).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87957 mRNA Translation: AAB47764.1
CH466524 Genomic DNA Translation: EDL37578.1
BC145872 mRNA Translation: AAI45873.1
BC145874 mRNA Translation: AAI45875.1
CCDSiCCDS19259.1
RefSeqiNP_031550.2, NM_007524.3
UniGeneiMm.2530

Genome annotation databases

EnsembliENSMUST00000060820; ENSMUSP00000051990; ENSMUSG00000049691
GeneIDi12020
KEGGimmu:12020
UCSCiuc008xhc.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNKX32_MOUSE
AccessioniPrimary (citable) accession number: P97503
Secondary accession number(s): A6H6G9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health