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P97501

- FMO3_MOUSE

UniProt

P97501 - FMO3_MOUSE

Protein

Dimethylaniline monooxygenase [N-oxide-forming] 3

Gene

Fmo3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Acts on TMA to produce TMA-N-oxide.1 Publication

    Catalytic activityi

    N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
    N,N,N-trimethylamine + NADPH + O2 = N,N,N-trimethylamine N-oxide + NADP+ + H2O.

    Cofactori

    FAD.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi9 – 146FADSequence Analysis
    Nucleotide bindingi191 – 1966NADPSequence Analysis

    GO - Molecular functioni

    1. amino acid binding Source: Ensembl
    2. flavin adenine dinucleotide binding Source: InterPro
    3. N,N-dimethylaniline monooxygenase activity Source: UniProtKB-EC
    4. NADP binding Source: Ensembl
    5. trimethylamine monooxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. drug metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 3 (EC:1.14.13.8)
    Alternative name(s):
    Dimethylaniline oxidase 3
    Hepatic flavin-containing monooxygenase 3
    Short name:
    FMO 3
    Trimethylamine monooxygenase (EC:1.14.13.148)
    Gene namesi
    Name:Fmo3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1100496. Fmo3.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 534533Dimethylaniline monooxygenase [N-oxide-forming] 3PRO_0000147656Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei401 – 4011Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP97501.
    PaxDbiP97501.
    PRIDEiP97501.

    PTM databases

    PhosphoSiteiP97501.

    Expressioni

    Tissue specificityi

    Liver.

    Inductioni

    Expression is specifically repressed in male mice after puberty, preventing trimethylamine degradation. Trimethylamine is present at high concentration in the urine of male mice after puberty and acts as an attractant.1 Publication

    Gene expression databases

    ArrayExpressiP97501.
    BgeeiP97501.
    CleanExiMM_FMO3.
    GenevestigatoriP97501.

    Interactioni

    Protein-protein interaction databases

    IntActiP97501. 3 interactions.
    MINTiMINT-4095189.
    STRINGi10090.ENSMUSP00000028010.

    Structurei

    3D structure databases

    ProteinModelPortaliP97501.
    SMRiP97501. Positions 2-208, 310-337.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FMO family.Curated

    Keywords - Domaini

    Transmembrane

    Phylogenomic databases

    eggNOGiCOG2072.
    HOGENOMiHOG000076537.
    HOVERGENiHBG002037.
    InParanoidiP97501.
    KOiK00485.
    OMAiFMHNSKL.
    OrthoDBiEOG7GXPB6.
    PhylomeDBiP97501.
    TreeFamiTF105285.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    InterProiIPR012143. DiMe-aniline_mOase.
    IPR000960. Flavin_mOase.
    IPR020946. Flavin_mOase-like.
    IPR002255. Flavin_mOase_3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00743. FMO-like. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000332. FMO. 1 hit.
    PRINTSiPR00370. FMOXYGENASE.
    PR01123. FMOXYGENASE3.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97501-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKKVAIIGA GVSGLAAIRS CLEEGLEPTC FERSDDVGGL WKFSDHIEEG    50
    RASIYQSVFT NSSKEMMCFP DFPYPDDFPN FMHHSKLQEY ITSFAKEKNL 100
    LKYIQFETPV TSINKCPNFS TTGKWEVTTE KHGKKETAVF DATMICSGHH 150
    IFPHVPKDSF PGLNRFKGKC FHSRDYKEPG IWKGKRVLVI GLGNSGCDIA 200
    AELSHVAQKV TISSRSGSWV MSRVWDDGYP WDMVVLTRFQ TFLKNNLPTA 250
    ISDWWYTRQM NARFKHENYG LVPLNRTLRK EPVFNDELPA RILCGMVTIK 300
    PNVKEFTETS AVFEDGTMFE AIDCVIFATG YGYAYPFLDD SIIKSRNNEV 350
    TLYKGVFPPQ LEKPTMAVIG LVQSLGATIP ITDLQARWAA QVIKGTCTLP 400
    SVNDMMDDID EKMGEKFKWY GNSTTIQTDY IVYMDELASF IGAKPNLLWL 450
    FLKDPRLAVE VFFGPCSPYQ FRLVGPGKWS GARNAILTQW DRSLKPMKTR 500
    VVSKVQKSCS HFYSRLLRLL AVPVLLIALF LVLI 534
    Length:534
    Mass (Da):60,516
    Last modified:May 1, 1997 - v1
    Checksum:iF72F7993C01AF9C9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87147 mRNA. Translation: AAB47541.1.
    CCDSiCCDS15426.1.
    RefSeqiNP_032056.1. NM_008030.1.
    UniGeneiMm.2900.

    Genome annotation databases

    EnsembliENSMUST00000028010; ENSMUSP00000028010; ENSMUSG00000026691.
    GeneIDi14262.
    KEGGimmu:14262.
    UCSCiuc007dhe.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87147 mRNA. Translation: AAB47541.1 .
    CCDSi CCDS15426.1.
    RefSeqi NP_032056.1. NM_008030.1.
    UniGenei Mm.2900.

    3D structure databases

    ProteinModelPortali P97501.
    SMRi P97501. Positions 2-208, 310-337.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P97501. 3 interactions.
    MINTi MINT-4095189.
    STRINGi 10090.ENSMUSP00000028010.

    PTM databases

    PhosphoSitei P97501.

    Proteomic databases

    MaxQBi P97501.
    PaxDbi P97501.
    PRIDEi P97501.

    Protocols and materials databases

    DNASUi 14262.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028010 ; ENSMUSP00000028010 ; ENSMUSG00000026691 .
    GeneIDi 14262.
    KEGGi mmu:14262.
    UCSCi uc007dhe.1. mouse.

    Organism-specific databases

    CTDi 2328.
    MGIi MGI:1100496. Fmo3.

    Phylogenomic databases

    eggNOGi COG2072.
    HOGENOMi HOG000076537.
    HOVERGENi HBG002037.
    InParanoidi P97501.
    KOi K00485.
    OMAi FMHNSKL.
    OrthoDBi EOG7GXPB6.
    PhylomeDBi P97501.
    TreeFami TF105285.

    Miscellaneous databases

    NextBioi 285607.
    PROi P97501.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97501.
    Bgeei P97501.
    CleanExi MM_FMO3.
    Genevestigatori P97501.

    Family and domain databases

    Gene3Di 3.40.50.720. 2 hits.
    InterProi IPR012143. DiMe-aniline_mOase.
    IPR000960. Flavin_mOase.
    IPR020946. Flavin_mOase-like.
    IPR002255. Flavin_mOase_3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00743. FMO-like. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000332. FMO. 1 hit.
    PRINTSi PR00370. FMOXYGENASE.
    PR01123. FMOXYGENASE3.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, sequencing, and expression in Escherichia coli of mouse flavin-containing monooxygenase 3 (FMO3): comparison with the human isoform."
      Falls J.G., Cherrington N.J., Clements K.M., Philpot R.M., Levi P.E., Rose R.L., Hodgson E.
      Arch. Biochem. Biophys. 347:9-18(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: CD-1.
      Tissue: Liver.
    2. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-401, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    3. "Synchronous evolution of an odor biosynthesis pathway and behavioral response."
      Li Q., Korzan W.J., Ferrero D.M., Chang R.B., Roy D.S., Buchi M., Lemon J.K., Kaur A.W., Stowers L., Fendt M., Liberles S.D.
      Curr. Biol. 23:11-20(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiFMO3_MOUSE
    AccessioniPrimary (citable) accession number: P97501
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Trimethylamine is a bacterial metabolite found in some animal odors, and is a repulsive odor associated with bad breath and spoiled food for most organisms, except for M.musculus, where it acts as an attractant.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3