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Protein

Dimethylaniline monooxygenase [N-oxide-forming] 3

Gene

Fmo3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Acts on TMA to produce TMA-N-oxide.1 Publication

Miscellaneous

Trimethylamine is a bacterial metabolite found in some animal odors, and is a repulsive odor associated with bad breath and spoiled food for most organisms, except for M.musculus, where it acts as an attractant.1 Publication

Catalytic activityi

N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
N,N,N-trimethylamine + NADPH + O2 = N,N,N-trimethylamine N-oxide + NADP+ + H2O.

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 13FADBy similarity5
Nucleotide bindingi40 – 41FADBy similarity2
Nucleotide bindingi60 – 61NADPBy similarity2
Nucleotide bindingi61 – 62FADBy similarity2
Nucleotide bindingi195 – 198NADPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

BRENDAi1.14.13.148 3474
1.14.13.8 3474
ReactomeiR-MMU-217271 FMO oxidises nucleophiles

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethylaniline monooxygenase [N-oxide-forming] 3 (EC:1.14.13.8)
Alternative name(s):
Dimethylaniline oxidase 3
Hepatic flavin-containing monooxygenase 3
Short name:
FMO 3
Trimethylamine monooxygenase (EC:1.14.13.148)
Gene namesi
Name:Fmo3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1100496 Fmo3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei514 – 534HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001476561 – 534Dimethylaniline monooxygenase [N-oxide-forming] 3Add BLAST534

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei401PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP97501
PeptideAtlasiP97501
PRIDEiP97501

PTM databases

iPTMnetiP97501
PhosphoSitePlusiP97501
SwissPalmiP97501

Expressioni

Tissue specificityi

Liver.

Inductioni

Expression is specifically repressed in male mice after puberty, preventing trimethylamine degradation. Trimethylamine is present at high concentration in the urine of male mice after puberty and acts as an attractant.1 Publication

Gene expression databases

BgeeiENSMUSG00000026691
CleanExiMM_FMO3
ExpressionAtlasiP97501 baseline and differential
GenevisibleiP97501 MM

Interactioni

Protein-protein interaction databases

IntActiP97501, 3 interactors
MINTiP97501
STRINGi10090.ENSMUSP00000028010

Structurei

3D structure databases

ProteinModelPortaliP97501
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FMO family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1399 Eukaryota
COG2072 LUCA
GeneTreeiENSGT00760000119232
HOGENOMiHOG000076537
HOVERGENiHBG002037
InParanoidiP97501
KOiK00485
OMAiAISDWWY
OrthoDBiEOG091G0465
PhylomeDBiP97501
TreeFamiTF105285

Family and domain databases

Gene3Di3.50.50.60, 4 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR000960 Flavin_mOase
IPR020946 Flavin_mOase-like
IPR002255 Flavin_mOase_3
PfamiView protein in Pfam
PF00743 FMO-like, 1 hit
PIRSFiPIRSF000332 FMO, 1 hit
PRINTSiPR00370 FMOXYGENASE
PR01123 FMOXYGENASE3
SUPFAMiSSF51905 SSF51905, 2 hits

Sequencei

Sequence statusi: Complete.

P97501-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKVAIIGA GVSGLAAIRS CLEEGLEPTC FERSDDVGGL WKFSDHIEEG
60 70 80 90 100
RASIYQSVFT NSSKEMMCFP DFPYPDDFPN FMHHSKLQEY ITSFAKEKNL
110 120 130 140 150
LKYIQFETPV TSINKCPNFS TTGKWEVTTE KHGKKETAVF DATMICSGHH
160 170 180 190 200
IFPHVPKDSF PGLNRFKGKC FHSRDYKEPG IWKGKRVLVI GLGNSGCDIA
210 220 230 240 250
AELSHVAQKV TISSRSGSWV MSRVWDDGYP WDMVVLTRFQ TFLKNNLPTA
260 270 280 290 300
ISDWWYTRQM NARFKHENYG LVPLNRTLRK EPVFNDELPA RILCGMVTIK
310 320 330 340 350
PNVKEFTETS AVFEDGTMFE AIDCVIFATG YGYAYPFLDD SIIKSRNNEV
360 370 380 390 400
TLYKGVFPPQ LEKPTMAVIG LVQSLGATIP ITDLQARWAA QVIKGTCTLP
410 420 430 440 450
SVNDMMDDID EKMGEKFKWY GNSTTIQTDY IVYMDELASF IGAKPNLLWL
460 470 480 490 500
FLKDPRLAVE VFFGPCSPYQ FRLVGPGKWS GARNAILTQW DRSLKPMKTR
510 520 530
VVSKVQKSCS HFYSRLLRLL AVPVLLIALF LVLI
Length:534
Mass (Da):60,516
Last modified:May 1, 1997 - v1
Checksum:iF72F7993C01AF9C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87147 mRNA Translation: AAB47541.1
CCDSiCCDS15426.1
RefSeqiNP_032056.1, NM_008030.2
UniGeneiMm.2900

Genome annotation databases

EnsembliENSMUST00000028010; ENSMUSP00000028010; ENSMUSG00000026691
GeneIDi14262
KEGGimmu:14262
UCSCiuc007dhe.1 mouse

Similar proteinsi

Entry informationi

Entry nameiFMO3_MOUSE
AccessioniPrimary (citable) accession number: P97501
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: April 25, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health