Reviewed, UniProtKB/Swiss-Prot P97501 (FMO3_MOUSE)

Names and origin

Protein names

Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 3
    EC=1.14.13.8
Alternative name(s):
    Hepatic flavin-containing monooxygenase 3
      Short name=FMO 3
    Dimethylaniline oxidase 3

Gene names

Name:

Fmo3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Acts on TMA to produce TMA-N-oxide By similarity.
Catalytic activity	N,N-dimethylaniline + NADPH + O(2) = N,N-dimethylaniline N-oxide + NADP(+) + H(2)O.
Cofactor	FAD By similarity.
Subcellular location	Microsome membrane. Endoplasmic reticulum membraneBy similarity.
Tissue specificity	Liver.
Sequence similarities	Belongs to the FMO family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from electronic annotation. Source: InterPro
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	Dimethylaniline monooxygenase [N-oxide-forming] 3		PRO_0000147656
Regions
	Nucleotide binding	9 – 14	6	FAD Potential
	Nucleotide binding	191 – 196	6	NADP Potential

Sequences

	Sequence		Length	Mass (Da)
	P97501-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: F72F7993C01AF9C9	FASTA	534	60,516
	10 20 30 40 50 60 MKKKVAIIGA GVSGLAAIRS CLEEGLEPTC FERSDDVGGL WKFSDHIEEG RASIYQSVFT 70 80 90 100 110 120 NSSKEMMCFP DFPYPDDFPN FMHHSKLQEY ITSFAKEKNL LKYIQFETPV TSINKCPNFS 130 140 150 160 170 180 TTGKWEVTTE KHGKKETAVF DATMICSGHH IFPHVPKDSF PGLNRFKGKC FHSRDYKEPG 190 200 210 220 230 240 IWKGKRVLVI GLGNSGCDIA AELSHVAQKV TISSRSGSWV MSRVWDDGYP WDMVVLTRFQ 250 260 270 280 290 300 TFLKNNLPTA ISDWWYTRQM NARFKHENYG LVPLNRTLRK EPVFNDELPA RILCGMVTIK 310 320 330 340 350 360 PNVKEFTETS AVFEDGTMFE AIDCVIFATG YGYAYPFLDD SIIKSRNNEV TLYKGVFPPQ 370 380 390 400 410 420 LEKPTMAVIG LVQSLGATIP ITDLQARWAA QVIKGTCTLP SVNDMMDDID EKMGEKFKWY 430 440 450 460 470 480 GNSTTIQTDY IVYMDELASF IGAKPNLLWL FLKDPRLAVE VFFGPCSPYQ FRLVGPGKWS 490 500 510 520 530 GARNAILTQW DRSLKPMKTR VVSKVQKSCS HFYSRLLRLL AVPVLLIALF LVLI

References

[1]	"Molecular cloning, sequencing, and expression in Escherichia coli of mouse flavin-containing monooxygenase 3 (FMO3): comparison with the human isoform." Falls J.G., Cherrington N.J., Clements K.M., Philpot R.M., Levi P.E., Rose R.L., Hodgson E. Arch. Biochem. Biophys. 347:9-18(1997) [PubMed: 9344459] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U87147 mRNA. Translation: AAB47541.1.
RefSeq	NP_032056.1.
UniGene	Mm.2900
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P97501.
Genome annotation databases
Ensembl	ENSMUSG00000026691. Mus musculus. [Contig view]
GeneID	14262.
KEGG	mmu:14262.
Organism-specific databases
MGI	MGI:1100496. Fmo3.
Phylogenomic databases
HOGENOM	P97501.
HOVERGEN	P97501.
Gene expression databases
ArrayExpress	P97501.
CleanEx	MM_FMO3.
GermOnline	ENSMUSG00000026691. Mus musculus.
Family and domain databases
InterPro	IPR000759. Adrndx_reductase. IPR012143. dManiline_mOase. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000960. Flavin_mOase. IPR002255. Flavin_mOase_3. IPR001100. Pyr_nuc-diS_OxRdtase. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
PIRSF	PIRSF000332. FMO. 1 hit.
PRINTS	PR00419. ADXRDTASE. PR00368. FADPNR. PR00370. FMOXYGENASE. PR01123. FMOXYGENASE3. PR00411. PNDRDTASEI.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	285607.
SOURCE	Search...

Entry information

Entry name

FMO3_MOUSE

Accession

Primary (citable) accession number: P97501

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	November 25, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)