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P97496 (SMRC1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SWI/SNF complex subunit SMARCC1
Alternative name(s):
BRG1-associated factor 155
SWI/SNF complex 155 kDa subunit
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1
SWI3-related protein
Short name=BAF155
Gene names
Name:Smarcc1
Synonyms:Baf155, Srg3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1104 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene By similarity. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Ref.3 Ref.4

Subunit structure

Component of 6 multiprotein chromatin-remodeling complexes: Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I), WINAC and Brg1(II). Each of the five complexes contains a catalytic subunit (either SMARCA4 or SMARCA2), and at least SMARCE1, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCC2 and SMARCB1. Other subunits specific to each of the complexes may also be present. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. May also interact with the SIN3A histone deacetylase transcription repressor complex in conjunction with SMARCA2 and SMARCA4. The minimal complex composed of SMARCC1 and SMARCA4 seems to be able to associate with cyclin such as CCNE1 or transcription factors such as KLF1 or GATA1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with NR3C1 and SMARD1. Interacts with TRIP12; leading to disrupt interaction between TRIP12 and SMARCE1 and prevent SMARCE1 ubiquitination By similarity. Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin. Ref.4

Subcellular location

Nucleus.

Tissue specificity

Highly expressed in adult brain, testis and thymus.

Developmental stage

Highly expressed in all organs except heart and liver (E12.5 and E14.5). The level of expression gradually diminishes as embryos develop, with expression restricted mostly to the CNS and thymus at E18.5. Expressed ubiquitously throughout the developing spinal cord, brain and other embryonic tissues at E10.5-E16.5. Ref.4

Sequence similarities

Belongs to the SMARCC family.

Contains 1 SANT domain.

Contains 1 SWIRM domain.

Sequence caution

The sequence AAB42085.1 differs from that shown. Reason: Frameshift at positions 5, 32, 48, 563, 565, 679 and 682.

Ontologies

Keywords
   Biological processNeurogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   Molecular functionChromatin regulator
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA packaging

Traceable author statement Ref.3. Source: MGI

chromatin remodeling

Inferred from electronic annotation. Source: Ensembl

insulin receptor signaling pathway

Inferred from direct assay PubMed 17074803. Source: MGI

nervous system development

Inferred from electronic annotation. Source: UniProtKB-KW

nucleosome disassembly

Inferred from electronic annotation. Source: Ensembl

organ morphogenesis

Inferred from mutant phenotype Ref.3. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 17074803. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentSWI/SNF complex

Inferred from direct assay PubMed 16287714PubMed 23643363. Source: MGI

WINAC complex

Inferred from electronic annotation. Source: Ensembl

XY body

Inferred from direct assay PubMed 16787967. Source: UniProtKB

intracellular

Inferred from direct assay Ref.3. Source: MGI

nBAF complex

Inferred from direct assay Ref.4. Source: UniProtKB

npBAF complex

Inferred from direct assay Ref.4. Source: UniProtKB

nucleus

Inferred from direct assay PubMed 16787967. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

chromatin binding

Inferred from direct assay PubMed 17074803. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Smarca4Q3TKT45EBI-648047,EBI-1210244
Smarcb1Q9Z0H35EBI-648047,EBI-689365

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P97496-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P97496-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1074-1104: YPPPPQQQQPPPPADGVPPPPAPGPPASATP → CK
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11041104SWI/SNF complex subunit SMARCC1
PRO_0000197116

Regions

Domain448 – 54598SWIRM
Domain617 – 66852SANT
Coiled coil909 – 94537 Potential
Compositional bias326 – 3338Poly-Pro
Compositional bias768 – 86295Glu-rich
Compositional bias866 – 87712Poly-Ala
Compositional bias977 – 1104128Pro-rich

Amino acid modifications

Modified residue3091Phosphoserine By similarity
Modified residue3271Phosphoserine Ref.5
Modified residue3291Phosphoserine Ref.5
Modified residue3341Phosphothreonine By similarity
Modified residue3441N6-acetyllysine By similarity
Modified residue3451N6-acetyllysine Ref.6
Modified residue3491Phosphoserine By similarity
Modified residue3531N6-acetyllysine By similarity
Modified residue3561Phosphoserine By similarity
Modified residue3581N6-acetyllysine By similarity
Modified residue3971Phosphothreonine By similarity
Modified residue5721Phosphoserine By similarity
Modified residue8211Phosphoserine By similarity
Modified residue8241Phosphoserine By similarity
Modified residue9471N6-acetyllysine By similarity

Natural variations

Alternative sequence1074 – 110431YPPPP…ASATP → CK in isoform 2.
VSP_012489

Experimental info

Sequence conflict1321G → S in AAB42085. Ref.1
Sequence conflict4681R → G in AAB42085. Ref.1
Sequence conflict4981R → C in AAB42085. Ref.1
Sequence conflict5121R → L in AAB42085. Ref.1
Sequence conflict8831L → P in AAB42085. Ref.1
Sequence conflict8911I → K in AAH53064. Ref.2
Sequence conflict9521R → L in AAH52423. Ref.2
Sequence conflict9841A → R in AAB42085. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 4, 2005. Version 2.
Checksum: 9030545104B90CAF

FASTA1,104122,890
        10         20         30         40         50         60 
MAATAGGGPG AAAGAVGAGG AAAASGLAVY RRKDGGPASK FWESPDTVSQ LDSVRVWLGK 

        70         80         90        100        110        120 
HYKKYVHADA PTNKTLAGLV VQLLQFQEDA FGKHVTNPAF TKLPAKCFMD FKAGGTLCHI 

       130        140        150        160        170        180 
LGAAYKYKNE QGWRRFDLQN PSRMDRNVEM FMNIEKTLVQ NNCLTRPNIY LIPDIDLKLA 

       190        200        210        220        230        240 
NKLKDIIKRH QGTFTDEKSK ASHHIYPYPS SQEDEEWLRP VMRRDKQVLV HWGFYPDSYD 

       250        260        270        280        290        300 
TWVHSNDVDA EIEDAPIPEK PWKVHVKWIL DTDVFNEWMN EEDYEVDENR KPVSFRQRIS 

       310        320        330        340        350        360 
TKNEEPVRSP ERRDRKASAN SRKRKPSPSP PPPTATESRK KSGKKGQASL YGKRRSQKEE 

       370        380        390        400        410        420 
DEQEDLTKDM EDPTPVPNIE EVVLPKNVNP KKDSENTPVK GGTVADLDEQ DEEAVTTGGK 

       430        440        450        460        470        480 
EDEDPSKGDP SRSVDPGEDN VTEQTNHIII PSYASWFDYN CIHVIERRAL PEFFNGKNKS 

       490        500        510        520        530        540 
KTPEIYLAYR NFMIDTYRLN PQEYLTSTAC RRNLTGDVCA VMRVHAFLEQ WGLVNYQVDP 

       550        560        570        580        590        600 
ESRPMAMGPP PTPHFNVLAD TPSGLVPLHL RSPQVPAAQQ MLNFPEKNKE KPIDLQNFGL 

       610        620        630        640        650        660 
RTDIYSKKTL AKSKGASAGR EWTEQETLLL LEALEMYKDD WNKVSEHVGS RTQDECILHF 

       670        680        690        700        710        720 
LRLPIEDPYL ENSDASLGPL AYQPVPFSQS GNPVMSTVAF LASVVDPRVA SAAAKAALEE 

       730        740        750        760        770        780 
FSRVREEVPL ELVEAHVKKV QEAARASGKV DPTYGLESSC IAGTGPDEPE KLEGSEEEKM 

       790        800        810        820        830        840 
ETDPDGQQPE KAENKVENES DEGDKIQDRE NEKNTEKEQD SDVSEDVKPE EKENEENKEL 

       850        860        870        880        890        900 
TDTCKERESD AGKKKVEHEI SEGNVATAAA AALASAATKA KHLAAVEERK IKSLVALLVE 

       910        920        930        940        950        960 
TQMKKLEIKL RHFEELETIM DREKEALEQQ RQQLLTERQN FHMEQLKYAE LRARQQMEQQ 

       970        980        990       1000       1010       1020 
QQHGQTPQQA HQHTGGPGMA PLGATGHPGM MPHQQPPPYP LMHHQMPPPH PPQPGQIPGP 

      1030       1040       1050       1060       1070       1080 
GSMMPGQPMP GRMIPAVAAN IHPTGSGPTP PGMPPMPGNI LGPRVPLTAP NGMYPPPPQQ 

      1090       1100 
QQPPPPADGV PPPPAPGPPA SATP 

« Hide

Isoform 2 [UniParc].

Checksum: DC6BDB5CD4C9A296
Show »

FASTA1,075120,091

References

« Hide 'large scale' references
[1]"A new mouse gene, SRG3, related to the SWI3 of Saccharomyces cerevisiae, is required for apoptosis induced by glucocorticoids in a thymoma cell line."
Jeon S.H., Kang M.G., Kim Y.H., Jin Y.H., Lee C., Chung H.-Y., Kwon H., Park S.D., Seong R.H.
J. Exp. Med. 185:1827-1836(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-892 (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
[3]"Srg3, a mouse homolog of yeast SWI3, is essential for early embryogenesis and involved in brain development."
Kim J.K., Huh S.-O., Choi H., Lee K.-S., Shin D., Lee C., Nam J.-S., Kim H., Chung H., Lee H.W., Park S.D., Seong R.H.
Mol. Cell. Biol. 21:7787-7795(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"An essential switch in subunit composition of a chromatin remodeling complex during neural development."
Lessard J., Wu J.I., Ranish J.A., Wan M., Winslow M.M., Staahl B.T., Wu H., Aebersold R., Graef I.A., Crabtree G.R.
Neuron 55:201-215(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE NBAF AND NPBAF COMPLEXES, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NBAF AND NPBAF COMPLEXES, DEVELOPMENTAL STAGE.
[5]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-327 AND SER-329, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[6]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U85614 mRNA. Translation: AAB42085.1. Frameshift.
BC052423 mRNA. Translation: AAH52423.1.
BC053064 mRNA. Translation: AAH53064.1.
PIRT30967.
RefSeqNP_033237.2. NM_009211.2.
UniGeneMm.85410.

3D structure databases

ProteinModelPortalP97496.
SMRP97496. Positions 454-538, 606-675.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203338. 18 interactions.
DIPDIP-39986N.
IntActP97496. 13 interactions.
MINTMINT-4084777.

PTM databases

PhosphoSiteP97496.

Proteomic databases

PaxDbP97496.
PRIDEP97496.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000088716; ENSMUSP00000086094; ENSMUSG00000032481. [P97496-1]
ENSMUST00000111969; ENSMUSP00000107600; ENSMUSG00000032481. [P97496-2]
GeneID20588.
KEGGmmu:20588.
UCSCuc009rto.1. mouse. [P97496-2]
uc009rtp.1. mouse. [P97496-1]

Organism-specific databases

CTD6599.
MGIMGI:1203524. Smarcc1.

Phylogenomic databases

eggNOGCOG5259.
GeneTreeENSGT00390000018166.
HOGENOMHOG000047736.
HOVERGENHBG054849.
InParanoidP97496.
KOK11649.
OMAYKKYVHA.
OrthoDBEOG7T4MJH.
PhylomeDBP97496.
TreeFamTF314710.

Gene expression databases

ArrayExpressP97496.
BgeeP97496.
GenevestigatorP97496.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProIPR001357. BRCT_dom.
IPR000953. Chromo_domain/shadow.
IPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR007526. SWIRM.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
[Graphical view]
SMARTSM00298. CHROMO. 1 hit.
SM00717. SANT. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 2 hits.
SSF52113. SSF52113. 2 hits.
PROSITEPS51293. SANT. 1 hit.
PS50934. SWIRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298891.
PROP97496.
SOURCESearch...

Entry information

Entry nameSMRC1_MOUSE
AccessionPrimary (citable) accession number: P97496
Secondary accession number(s): Q7TS80, Q7TT29
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot