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Protein

Glutamate--cysteine ligase catalytic subunit

Gene

Gclc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.

Enzyme regulationi

Feedback inhibition by glutathione.

Pathwayi

GO - Molecular functioni

  1. ADP binding Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. coenzyme binding Source: UniProtKB
  4. glutamate binding Source: UniProtKB
  5. glutamate-cysteine ligase activity Source: UniProtKB
  6. magnesium ion binding Source: UniProtKB
  7. protein heterodimerization activity Source: MGI

GO - Biological processi

  1. apoptotic mitochondrial changes Source: MGI
  2. cell redox homeostasis Source: UniProtKB
  3. cysteine metabolic process Source: UniProtKB
  4. glutamate metabolic process Source: UniProtKB
  5. glutathione biosynthetic process Source: UniProtKB
  6. glutathione metabolic process Source: MGI
  7. L-ascorbic acid metabolic process Source: MGI
  8. negative regulation of apoptotic process Source: UniProtKB
  9. negative regulation of extrinsic apoptotic signaling pathway Source: MGI
  10. negative regulation of neuron apoptotic process Source: Ensembl
  11. negative regulation of protein ubiquitination Source: MGI
  12. negative regulation of transcription, DNA-templated Source: UniProtKB
  13. positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: MGI
  14. regulation of blood vessel size Source: UniProtKB
  15. regulation of mitochondrial depolarization Source: MGI
  16. response to arsenic-containing substance Source: MGI
  17. response to heat Source: UniProtKB
  18. response to hormone Source: UniProtKB
  19. response to nitrosative stress Source: Ensembl
  20. response to oxidative stress Source: UniProtKB
  21. response to xenobiotic stimulus Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.2.2. 3474.
ReactomeiREACT_277860. Sulfur amino acid metabolism.
REACT_319124. Glutathione synthesis and recycling.
SABIO-RKP97494.
UniPathwayiUPA00142; UER00209.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--cysteine ligase catalytic subunit (EC:6.3.2.2)
Alternative name(s):
GCS heavy chain
Gamma-ECS
Gamma-glutamylcysteine synthetase
Gene namesi
Name:Gclc
Synonyms:Glclc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:104990. Gclc.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: MGI
  2. glutamate-cysteine ligase complex Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637Glutamate--cysteine ligase catalytic subunitPRO_0000192564Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei5 – 51PhosphoserineBy similarity
Modified residuei8 – 81PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP97494.
PaxDbiP97494.
PRIDEiP97494.

PTM databases

PhosphoSiteiP97494.

Expressioni

Gene expression databases

BgeeiP97494.
CleanExiMM_GCLC.
ExpressionAtlasiP97494. baseline and differential.
GenevestigatoriP97494.

Interactioni

Subunit structurei

Heterodimer of a catalytic heavy chain and a regulatory light chain.

Protein-protein interaction databases

BioGridi199940. 1 interaction.
IntActiP97494. 2 interactions.
MINTiMINT-1869507.

Structurei

3D structure databases

ProteinModelPortaliP97494.
SMRiP97494. Positions 2-621.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG269969.
HOGENOMiHOG000199354.
HOVERGENiHBG005924.
InParanoidiP97494.
KOiK11204.
OMAiRWMREFI.
OrthoDBiEOG7B8S3B.
PhylomeDBiP97494.
TreeFamiTF105644.

Family and domain databases

InterProiIPR004308. GCS.
[Graphical view]
PANTHERiPTHR11164. PTHR11164. 1 hit.
PfamiPF03074. GCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97494-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLSQGSPL SWEETQRHAD HVRRHGILQF LHIYHAVKDR HKDVLKWGDE
60 70 80 90 100
VEYMLVSFDH ENRKVQLLLN GGDVLETLQE KGERTNPNHP TLWRPEYGSY
110 120 130 140 150
MIEGTPGQPY GGTMSEFNTV EANMRKRRKE ATSVLGEHQA LCTITSFPRL
160 170 180 190 200
GCPGFTLPEH RPNPEEGGAS KSLFFPDEAI NKHPRFGTLT RNIRHRRGEK
210 220 230 240 250
VVINVPIFKD KNTPSPFVET FPEDAEASKA SQPDHIYMDA MGFGMGNCCL
260 270 280 290 300
QVTFQACSIS EARYLYDQLA TICPIVMALS AASPFYRGYV SDIDCRWGVI
310 320 330 340 350
SASVDDRTRE ERGLEPLKNN RFRISKSRYD SIDSYLSKCG EKYNDIDLTI
360 370 380 390 400
DKEIYEQLLE EGIDHLLAQH VAHLFIRDPL TLFEEKIHLD DANESDHFEN
410 420 430 440 450
IQSTNWQTMR FKPPPPNSDI GWRVEFRPME VQLTDFENSA YVVFVVLLTR
460 470 480 490 500
VILSYKLDFL IPLSKVDENM KVAQKRDAVL QGMFYFRKDI CKGGNAVVDG
510 520 530 540 550
CSKAQSSSEP AAEEYTLMSI DTIINGKEGV FPGLIPILNS YLENMEVDVD
560 570 580 590 600
TRCSILNYLK LIKKRASGEL MTVARWMREF IANHPDYKQD SVITDEINYS
610 620 630
LIWKCNQIAD ELCECPELLG SGFRKAKYSG GKSDPSA
Length:637
Mass (Da):72,571
Last modified:January 22, 2007 - v4
Checksum:i8A176CD1DE49094C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61Q → H in AAB42020 (PubMed:9223101).Curated
Sequence conflicti25 – 262HG → RD in AAB42020 (PubMed:9223101).Curated
Sequence conflicti32 – 321H → Q in AAH19374 (PubMed:15489334).Curated
Sequence conflicti500 – 5001G → E in AAB42020 (PubMed:9223101).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85414 mRNA. Translation: AAB42020.1.
U85498 mRNA. Translation: AAB52542.1.
BC019374 mRNA. Translation: AAH19374.1.
CCDSiCCDS23354.1.
RefSeqiNP_034425.1. NM_010295.2.
UniGeneiMm.89888.

Genome annotation databases

EnsembliENSMUST00000034905; ENSMUSP00000034905; ENSMUSG00000032350.
GeneIDi14629.
KEGGimmu:14629.
UCSCiuc009qtm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85414 mRNA. Translation: AAB42020.1.
U85498 mRNA. Translation: AAB52542.1.
BC019374 mRNA. Translation: AAH19374.1.
CCDSiCCDS23354.1.
RefSeqiNP_034425.1. NM_010295.2.
UniGeneiMm.89888.

3D structure databases

ProteinModelPortaliP97494.
SMRiP97494. Positions 2-621.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199940. 1 interaction.
IntActiP97494. 2 interactions.
MINTiMINT-1869507.

Chemistry

ChEMBLiCHEMBL2366483.

PTM databases

PhosphoSiteiP97494.

Proteomic databases

MaxQBiP97494.
PaxDbiP97494.
PRIDEiP97494.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034905; ENSMUSP00000034905; ENSMUSG00000032350.
GeneIDi14629.
KEGGimmu:14629.
UCSCiuc009qtm.2. mouse.

Organism-specific databases

CTDi2729.
MGIiMGI:104990. Gclc.

Phylogenomic databases

eggNOGiNOG269969.
HOGENOMiHOG000199354.
HOVERGENiHBG005924.
InParanoidiP97494.
KOiK11204.
OMAiRWMREFI.
OrthoDBiEOG7B8S3B.
PhylomeDBiP97494.
TreeFamiTF105644.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209.
BRENDAi6.3.2.2. 3474.
ReactomeiREACT_277860. Sulfur amino acid metabolism.
REACT_319124. Glutathione synthesis and recycling.
SABIO-RKP97494.

Miscellaneous databases

ChiTaRSiGclc. mouse.
NextBioi286474.
PROiP97494.
SOURCEiSearch...

Gene expression databases

BgeeiP97494.
CleanExiMM_GCLC.
ExpressionAtlasiP97494. baseline and differential.
GenevestigatoriP97494.

Family and domain databases

InterProiIPR004308. GCS.
[Graphical view]
PANTHERiPTHR11164. PTHR11164. 1 hit.
PfamiPF03074. GCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning/brain localization of mouse glutamylcysteine synthetase heavy chain mRNA."
    Kang Y., Oiao X., Jurma O., Knusel B., Andersen J.K.
    NeuroReport 8:2053-2060(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Molecular cloning and sequencing of the cDNA encoding the catalytic subunit of mouse glutamate-cysteine ligase."
    Reid L.L., Botta D., Lu Y., Gallagher E.P., Kavanagh T.J.
    Biochim. Biophys. Acta 1352:233-237(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiGSH1_MOUSE
AccessioniPrimary (citable) accession number: P97494
Secondary accession number(s): O09166, Q8VCS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: January 22, 2007
Last modified: March 31, 2015
This is version 123 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.