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Protein

Adenylate cyclase type 8

Gene

Adcy8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This is a membrane-bound, calcium-stimulable adenylyl cyclase. May be involved in learning, in memory and in drug dependence (By similarity).By similarity

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Enzyme regulationi

Activated by calcium/calmodulin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi417 – 4171Magnesium 1PROSITE-ProRule annotation
Metal bindingi417 – 4171Magnesium 2PROSITE-ProRule annotation
Metal bindingi418 – 4181Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi461 – 4611Magnesium 1PROSITE-ProRule annotation
Metal bindingi461 – 4611Magnesium 2PROSITE-ProRule annotation

GO - Molecular functioni

  • adenylate cyclase activity Source: MGI
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • long-term memory Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cAMP biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.1. 3474.
ReactomeiREACT_278699. Hedgehog 'off' state.
REACT_286837. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_295338. PKA activation in glucagon signalling.
REACT_296380. G alpha (z) signalling events.
REACT_297425. Adenylate cyclase activating pathway.
REACT_297430. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_303391. CREB phosphorylation through the activation of Adenylate Cyclase.
REACT_309686. Adenylate cyclase inhibitory pathway.
REACT_310977. PKA activation.
REACT_313192. G alpha (s) signalling events.
REACT_331048. G alpha (i) signalling events.
REACT_345203. Glucagon signaling in metabolic regulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate cyclase type 8 (EC:4.6.1.1)
Alternative name(s):
ATP pyrophosphate-lyase 8
Adenylate cyclase type VIII
Adenylyl cyclase 8
Ca(2+)/calmodulin-activated adenylyl cyclase
Gene namesi
Name:Adcy8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1341110. Adcy8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 180180CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei181 – 20121HelicalSequence AnalysisAdd
BLAST
Transmembranei210 – 23021HelicalSequence AnalysisAdd
BLAST
Transmembranei245 – 26521HelicalSequence AnalysisAdd
BLAST
Transmembranei272 – 29221HelicalSequence AnalysisAdd
BLAST
Transmembranei294 – 31421HelicalSequence AnalysisAdd
BLAST
Transmembranei319 – 33921HelicalSequence AnalysisAdd
BLAST
Topological domaini340 – 713374CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei714 – 73421HelicalSequence AnalysisAdd
BLAST
Transmembranei736 – 75621HelicalSequence AnalysisAdd
BLAST
Transmembranei785 – 80521HelicalSequence AnalysisAdd
BLAST
Transmembranei829 – 84921HelicalSequence AnalysisAdd
BLAST
Transmembranei859 – 87921HelicalSequence AnalysisAdd
BLAST
Transmembranei892 – 91221HelicalSequence AnalysisAdd
BLAST
Topological domaini913 – 1249337CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12491249Adenylate cyclase type 8PRO_0000195706Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi815 – 8151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi819 – 8191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi886 – 8861N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP97490.
PRIDEiP97490.

PTM databases

PhosphoSiteiP97490.

Expressioni

Gene expression databases

CleanExiMM_ADCY8.

Interactioni

Protein-protein interaction databases

BioGridi197977. 4 interactions.
MINTiMINT-4997019.
STRINGi10090.ENSMUSP00000023007.

Structurei

3D structure databases

ProteinModelPortaliP97490.
SMRiP97490. Positions 400-585, 969-1143.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 2 guanylate cyclase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2114.
GeneTreeiENSGT00760000119042.
HOGENOMiHOG000006941.
HOVERGENiHBG050458.
InParanoidiP97490.
KOiK08048.
OMAiVVLFMCM.
OrthoDBiEOG7B8S30.
TreeFamiTF313845.

Family and domain databases

Gene3Di3.30.70.1230. 2 hits.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR030672. Adcy.
IPR009398. Adcy_conserved_dom.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF06327. DUF1053. 1 hit.
PF00211. Guanylate_cyc. 2 hits.
[Graphical view]
PIRSFiPIRSF039050. Ade_cyc. 1 hit.
SMARTiSM00044. CYCc. 2 hits.
[Graphical view]
SUPFAMiSSF55073. SSF55073. 2 hits.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 2 hits.
PS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELSDVHCLS GSEELYTIQP TPPAGDDGSG SRPQRLLWQT AVRHITEQRF
60 70 80 90 100
IHGHRGGGGG GVSRKASNPA GSGPNHHAPQ LSSDSVLPLY SLGPGERAHN
110 120 130 140 150
TGGTKVFPER SGSGSASGSG GGGDLGFLHL DCAPSNSDFF LNGGYSYRGV
160 170 180 190 200
IFPTLRNSFK SRDLERLYQR YFLGQRRKSE VVMNVLDVLT KLTLLVLHLS
210 220 230 240 250
LASAPMDPLK GILLGFFTGI EVVICALVVV RKDNTSHTYL QYSGVVTWVA
260 270 280 290 300
MTTQILAAGL GYGLLGDGIG YVLFTLFATY SMLPLPLTWA ILAGLGTSLL
310 320 330 340 350
QVTLQVLIPR LAVFSINQVL AQVVLFMCMN TAGIFISYLS DRAQRQAFLE
360 370 380 390 400
TRRCVEARLR LETENQRQER LVLSVLPRFV VLEMINDMTN VEDEHLQHQF
410 420 430 440 450
HRIYIHRYEN VSILFADVKG FTNLSTTLSA QELVRMLNEL FARFDRLAHE
460 470 480 490 500
HHCLRIKILG DCYYCVSGLP EPRRDHAHCC VEMGLSMIKT IRFVRSRTKH
510 520 530 540 550
DVDMRIGIHS GSVLCGVLGL RKWQFDVWSW DVDIANKLES GGIPGRIHIS
560 570 580 590 600
KATLDCLNGD YNVEEGHGKE RNEFLRKHNI ETYLIKQPEE SLLCLPEDIV
610 620 630 640 650
KESVSCSDRR NSGATFTEGS WSPELPFDNI VGKQNTLAAL TRNSINLLPN
660 670 680 690 700
HLAQALHVQS GPEEINKRIE HTIDLRSGDK LRREHIKPFS LMFKDSSLEH
710 720 730 740 750
KYSQMRDEVF KSNLVCAFIV LLFITAIQSL LPSSRLMPMT IQFSILIMLH
760 770 780 790 800
SALVLITTAE DYKCLPLILR KTCCWINETY LARNVIIFAS ILINFLGAVL
810 820 830 840 850
NILWCDFDKS IPLKNLTFNS SAVFTDICSY PEYFVFTGVL AMVTCAVFLR
860 870 880 890 900
LNSVLKLAVL LIMIAIYALL TETIYAGLFL SYDNLNHSGE DFLGTKEASL
910 920 930 940 950
LLMAMFLLAV FYHGQQLEYT ARLDFLWRVQ AKEEINEMKE LREHNENMLR
960 970 980 990 1000
NILPSHVARH FLEKDRDNEE LYSQSYDAVG VMFASIPGFA DFYSQTEMNN
1010 1020 1030 1040 1050
QGVECLRLLN EIIADFDELL GEDRFQDIEK IKTIGSTYMA VSGLSPEKQQ
1060 1070 1080 1090 1100
CEDKWGHLCA LADFSLALTE SIQEINKHSF NNFELRIGIS HGSVVAGVIG
1110 1120 1130 1140 1150
AKKPQYDIWG KTVNLASRMD STGVSGRIQV PEETYLILKD QGFAFDYRGE
1160 1170 1180 1190 1200
IYVKGISEQE GKIKTYFLLG RVQPNPFILP PRRLPGQYSL AAVVLGLVQS
1210 1220 1230 1240
LNRQRQKQLL NENSNSGIIK SHYNRRTLLT PSGPEPGAQA EGTDKSDLP
Length:1,249
Mass (Da):140,095
Last modified:October 3, 2012 - v2
Checksum:i26103B6E0DB9C701
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241A → V in AAB41885 (Ref. 1) Curated
Sequence conflicti59 – 591G → C in AAB41885 (Ref. 1) Curated
Sequence conflicti849 – 8491L → V in AAB41885 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85021 mRNA. Translation: AAB41885.1.
AC116996 Genomic DNA. No translation available.
AC160934 Genomic DNA. No translation available.
CH466545 Genomic DNA. Translation: EDL29360.1.
CH466545 Genomic DNA. Translation: EDL29361.1.
CCDSiCCDS27506.1.
RefSeqiNP_033753.2. NM_009623.2.
UniGeneiMm.1425.

Genome annotation databases

EnsembliENSMUST00000023007; ENSMUSP00000023007; ENSMUSG00000022376.
GeneIDi11514.
KEGGimmu:11514.
UCSCiuc007vzo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85021 mRNA. Translation: AAB41885.1.
AC116996 Genomic DNA. No translation available.
AC160934 Genomic DNA. No translation available.
CH466545 Genomic DNA. Translation: EDL29360.1.
CH466545 Genomic DNA. Translation: EDL29361.1.
CCDSiCCDS27506.1.
RefSeqiNP_033753.2. NM_009623.2.
UniGeneiMm.1425.

3D structure databases

ProteinModelPortaliP97490.
SMRiP97490. Positions 400-585, 969-1143.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197977. 4 interactions.
MINTiMINT-4997019.
STRINGi10090.ENSMUSP00000023007.

PTM databases

PhosphoSiteiP97490.

Proteomic databases

MaxQBiP97490.
PRIDEiP97490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023007; ENSMUSP00000023007; ENSMUSG00000022376.
GeneIDi11514.
KEGGimmu:11514.
UCSCiuc007vzo.1. mouse.

Organism-specific databases

CTDi114.
MGIiMGI:1341110. Adcy8.

Phylogenomic databases

eggNOGiCOG2114.
GeneTreeiENSGT00760000119042.
HOGENOMiHOG000006941.
HOVERGENiHBG050458.
InParanoidiP97490.
KOiK08048.
OMAiVVLFMCM.
OrthoDBiEOG7B8S30.
TreeFamiTF313845.

Enzyme and pathway databases

BRENDAi4.6.1.1. 3474.
ReactomeiREACT_278699. Hedgehog 'off' state.
REACT_286837. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_295338. PKA activation in glucagon signalling.
REACT_296380. G alpha (z) signalling events.
REACT_297425. Adenylate cyclase activating pathway.
REACT_297430. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_303391. CREB phosphorylation through the activation of Adenylate Cyclase.
REACT_309686. Adenylate cyclase inhibitory pathway.
REACT_310977. PKA activation.
REACT_313192. G alpha (s) signalling events.
REACT_331048. G alpha (i) signalling events.
REACT_345203. Glucagon signaling in metabolic regulation.

Miscellaneous databases

NextBioi278926.
PROiP97490.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ADCY8.

Family and domain databases

Gene3Di3.30.70.1230. 2 hits.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR030672. Adcy.
IPR009398. Adcy_conserved_dom.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF06327. DUF1053. 1 hit.
PF00211. Guanylate_cyc. 2 hits.
[Graphical view]
PIRSFiPIRSF039050. Ade_cyc. 1 hit.
SMARTiSM00044. CYCc. 2 hits.
[Graphical view]
SUPFAMiSSF55073. SSF55073. 2 hits.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 2 hits.
PS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of mouse adenylyl cyclase type 8."
    Premont R.T.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiADCY8_MOUSE
AccessioniPrimary (citable) accession number: P97490
Secondary accession number(s): G3X8V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 3, 2012
Last modified: June 24, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.