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Protein

Adenylate cyclase type 8

Gene

Adcy8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is a membrane-bound, calcium-stimulable adenylyl cyclase. May be involved in learning, in memory and in drug dependence (By similarity).By similarity

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions per subunit. Is also active with manganese (in vitro).By similarity

Enzyme regulationi

Activated by calcium/calmodulin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi417Magnesium 1; catalyticPROSITE-ProRule annotation1
Metal bindingi417Magnesium 2; catalyticPROSITE-ProRule annotation1
Metal bindingi418Magnesium 2; via carbonyl oxygen; catalyticPROSITE-ProRule annotation1
Metal bindingi461Magnesium 1; catalyticPROSITE-ProRule annotation1
Metal bindingi461Magnesium 2; catalyticPROSITE-ProRule annotation1
Binding sitei505ATPBy similarity1
Binding sitei1032ATPBy similarity1
Binding sitei1154ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi417 – 422ATPBy similarity6
Nucleotide bindingi459 – 461ATPBy similarity3
Nucleotide bindingi1107 – 1109ATPBy similarity3
Nucleotide bindingi1114 – 1118ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processcAMP biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.1 3474
ReactomeiR-MMU-163359 Glucagon signaling in metabolic regulation
R-MMU-163615 PKA activation
R-MMU-164378 PKA activation in glucagon signalling
R-MMU-170660 Adenylate cyclase activating pathway
R-MMU-170670 Adenylate cyclase inhibitory pathway
R-MMU-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-MMU-418555 G alpha (s) signalling events
R-MMU-418597 G alpha (z) signalling events
R-MMU-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-MMU-442720 CREB phosphorylation through the activation of Adenylate Cyclase
R-MMU-5610787 Hedgehog 'off' state

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate cyclase type 8 (EC:4.6.1.1)
Alternative name(s):
ATP pyrophosphate-lyase 8
Adenylate cyclase type VIII
Adenylyl cyclase 8
Ca(2+)/calmodulin-activated adenylyl cyclase
Gene namesi
Name:Adcy8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1341110 Adcy8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 180CytoplasmicSequence analysisAdd BLAST180
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Transmembranei210 – 230HelicalSequence analysisAdd BLAST21
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Transmembranei272 – 292HelicalSequence analysisAdd BLAST21
Transmembranei294 – 314HelicalSequence analysisAdd BLAST21
Transmembranei319 – 339HelicalSequence analysisAdd BLAST21
Topological domaini340 – 713CytoplasmicSequence analysisAdd BLAST374
Transmembranei714 – 734HelicalSequence analysisAdd BLAST21
Transmembranei736 – 756HelicalSequence analysisAdd BLAST21
Transmembranei785 – 805HelicalSequence analysisAdd BLAST21
Transmembranei829 – 849HelicalSequence analysisAdd BLAST21
Transmembranei859 – 879HelicalSequence analysisAdd BLAST21
Transmembranei892 – 912HelicalSequence analysisAdd BLAST21
Topological domaini913 – 1249CytoplasmicSequence analysisAdd BLAST337

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001957061 – 1249Adenylate cyclase type 8Add BLAST1249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55Omega-N-methylarginineCombined sources1
Modified residuei612PhosphoserineCombined sources1
Modified residuei622PhosphoserineCombined sources1
Glycosylationi815N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi819N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi886N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

MaxQBiP97490
PaxDbiP97490
PeptideAtlasiP97490
PRIDEiP97490

PTM databases

iPTMnetiP97490
PhosphoSitePlusiP97490

Expressioni

Gene expression databases

BgeeiENSMUSG00000022376
CleanExiMM_ADCY8
GenevisibleiP97490 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi197977, 4 interactors
CORUMiP97490
STRINGi10090.ENSMUSP00000023007

Structurei

3D structure databases

ProteinModelPortaliP97490
SMRiP97490
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The protein contains two modules with six transmembrane helices each; both are required for catalytic activity. Isolated N-terminal or C-terminal guanylate cyclase domains have no catalytic activity, but when they are brought together, enzyme activity is restored. The active site is at the interface of the two domains. Both contribute substrate-binding residues, but the catalytic metal ions are bound exclusively via the N-terminal guanylate cyclase domain.By similarity

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3619 Eukaryota
COG2114 LUCA
GeneTreeiENSGT00760000119042
HOGENOMiHOG000006941
HOVERGENiHBG050458
InParanoidiP97490
KOiK08048
OMAiMAIQFSI
OrthoDBiEOG091G05JR
TreeFamiTF313845

Family and domain databases

Gene3Di3.30.70.1230, 2 hits
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR018297 A/G_cyclase_CS
IPR032628 AC_N
IPR030672 Adcy
IPR009398 Adcy_conserved_dom
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF16214 AC_N, 1 hit
PF06327 DUF1053, 1 hit
PF00211 Guanylate_cyc, 2 hits
PIRSFiPIRSF039050 Ade_cyc, 1 hit
SMARTiView protein in SMART
SM00044 CYCc, 2 hits
SUPFAMiSSF55073 SSF55073, 2 hits
PROSITEiView protein in PROSITE
PS00452 GUANYLATE_CYCLASE_1, 2 hits
PS50125 GUANYLATE_CYCLASE_2, 2 hits

Sequencei

Sequence statusi: Complete.

P97490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELSDVHCLS GSEELYTIQP TPPAGDDGSG SRPQRLLWQT AVRHITEQRF
60 70 80 90 100
IHGHRGGGGG GVSRKASNPA GSGPNHHAPQ LSSDSVLPLY SLGPGERAHN
110 120 130 140 150
TGGTKVFPER SGSGSASGSG GGGDLGFLHL DCAPSNSDFF LNGGYSYRGV
160 170 180 190 200
IFPTLRNSFK SRDLERLYQR YFLGQRRKSE VVMNVLDVLT KLTLLVLHLS
210 220 230 240 250
LASAPMDPLK GILLGFFTGI EVVICALVVV RKDNTSHTYL QYSGVVTWVA
260 270 280 290 300
MTTQILAAGL GYGLLGDGIG YVLFTLFATY SMLPLPLTWA ILAGLGTSLL
310 320 330 340 350
QVTLQVLIPR LAVFSINQVL AQVVLFMCMN TAGIFISYLS DRAQRQAFLE
360 370 380 390 400
TRRCVEARLR LETENQRQER LVLSVLPRFV VLEMINDMTN VEDEHLQHQF
410 420 430 440 450
HRIYIHRYEN VSILFADVKG FTNLSTTLSA QELVRMLNEL FARFDRLAHE
460 470 480 490 500
HHCLRIKILG DCYYCVSGLP EPRRDHAHCC VEMGLSMIKT IRFVRSRTKH
510 520 530 540 550
DVDMRIGIHS GSVLCGVLGL RKWQFDVWSW DVDIANKLES GGIPGRIHIS
560 570 580 590 600
KATLDCLNGD YNVEEGHGKE RNEFLRKHNI ETYLIKQPEE SLLCLPEDIV
610 620 630 640 650
KESVSCSDRR NSGATFTEGS WSPELPFDNI VGKQNTLAAL TRNSINLLPN
660 670 680 690 700
HLAQALHVQS GPEEINKRIE HTIDLRSGDK LRREHIKPFS LMFKDSSLEH
710 720 730 740 750
KYSQMRDEVF KSNLVCAFIV LLFITAIQSL LPSSRLMPMT IQFSILIMLH
760 770 780 790 800
SALVLITTAE DYKCLPLILR KTCCWINETY LARNVIIFAS ILINFLGAVL
810 820 830 840 850
NILWCDFDKS IPLKNLTFNS SAVFTDICSY PEYFVFTGVL AMVTCAVFLR
860 870 880 890 900
LNSVLKLAVL LIMIAIYALL TETIYAGLFL SYDNLNHSGE DFLGTKEASL
910 920 930 940 950
LLMAMFLLAV FYHGQQLEYT ARLDFLWRVQ AKEEINEMKE LREHNENMLR
960 970 980 990 1000
NILPSHVARH FLEKDRDNEE LYSQSYDAVG VMFASIPGFA DFYSQTEMNN
1010 1020 1030 1040 1050
QGVECLRLLN EIIADFDELL GEDRFQDIEK IKTIGSTYMA VSGLSPEKQQ
1060 1070 1080 1090 1100
CEDKWGHLCA LADFSLALTE SIQEINKHSF NNFELRIGIS HGSVVAGVIG
1110 1120 1130 1140 1150
AKKPQYDIWG KTVNLASRMD STGVSGRIQV PEETYLILKD QGFAFDYRGE
1160 1170 1180 1190 1200
IYVKGISEQE GKIKTYFLLG RVQPNPFILP PRRLPGQYSL AAVVLGLVQS
1210 1220 1230 1240
LNRQRQKQLL NENSNSGIIK SHYNRRTLLT PSGPEPGAQA EGTDKSDLP
Length:1,249
Mass (Da):140,095
Last modified:October 3, 2012 - v2
Checksum:i26103B6E0DB9C701
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24A → V in AAB41885 (Ref. 1) Curated1
Sequence conflicti59G → C in AAB41885 (Ref. 1) Curated1
Sequence conflicti849L → V in AAB41885 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85021 mRNA Translation: AAB41885.1
AC116996 Genomic DNA No translation available.
AC160934 Genomic DNA No translation available.
CH466545 Genomic DNA Translation: EDL29360.1
CH466545 Genomic DNA Translation: EDL29361.1
CCDSiCCDS27506.1
RefSeqiNP_033753.2, NM_009623.2
UniGeneiMm.1425

Genome annotation databases

EnsembliENSMUST00000023007; ENSMUSP00000023007; ENSMUSG00000022376
GeneIDi11514
KEGGimmu:11514
UCSCiuc007vzo.1 mouse

Similar proteinsi

Entry informationi

Entry nameiADCY8_MOUSE
AccessioniPrimary (citable) accession number: P97490
Secondary accession number(s): G3X8V9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 3, 2012
Last modified: June 20, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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