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Protein

Leukocyte immunoglobulin-like receptor subfamily B member 3

Gene

Lilrb3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. Down-regulates antigen-induced B-cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM).3 Publications

GO - Biological processi

  • B cell homeostasis Source: MGI
  • B cell mediated immunity Source: MGI
  • cytokine-mediated signaling pathway Source: MGI
  • myeloid dendritic cell differentiation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Leukocyte immunoglobulin-like receptor subfamily B member 3
Short name:
LIR-3
Short name:
Leukocyte immunoglobulin-like receptor 3
Alternative name(s):
Cell-surface glycoprotein p91
Paired immunoglobulin-like receptor B
Short name:
PIR-B
Gene namesi
Name:Lilrb3
Synonyms:Pirb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:894311. Lilrb3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 642618ExtracellularSequence analysisAdd
BLAST
Transmembranei643 – 66321HelicalSequence analysisAdd
BLAST
Topological domaini664 – 841178CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi794 – 7941Y → F: Abolishes interaction with PTPN6/SHP-1 and PTPN11/SHP-2; when associated with F-824. 2 Publications
Mutagenesisi824 – 8241Y → F: Abolishes interaction with PTPN6/SHP-1 and PTPN11/SHP-2; when associated with F-794. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 841817Leukocyte immunoglobulin-like receptor subfamily B member 3PRO_0000414708Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 98PROSITE-ProRule annotation
Disulfide bondi246 ↔ 295PROSITE-ProRule annotation
Glycosylationi338 – 3381N-linked (GlcNAc...)Sequence analysis
Disulfide bondi343 ↔ 395PROSITE-ProRule annotation
Disulfide bondi444 ↔ 495PROSITE-ProRule annotation
Glycosylationi500 – 5001N-linked (GlcNAc...)Sequence analysis
Disulfide bondi544 ↔ 595PROSITE-ProRule annotation
Modified residuei684 – 6841PhosphoserineCombined sources
Modified residuei757 – 7571PhosphoserineCombined sources
Modified residuei794 – 7941Phosphotyrosine; by LYN1 Publication
Modified residuei824 – 8241Phosphotyrosine; by LYN1 Publication

Post-translational modificationi

Phosphorylated on tyrosine residues by LYN. Phosphorylation at Tyr-794 and Tyr-824 is important for interaction with PTPN6/SHP-1 and PTPN11/SHP-2.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP97484.
PaxDbiP97484.
PRIDEiP97484.

PTM databases

iPTMnetiP97484.
PhosphoSiteiP97484.

Expressioni

Tissue specificityi

Detected in macrophages, splenocytes and B lymphocytes (at protein level). Detected in macrophages, mast cells, splenocytes, peritoneal cells and natural killer cells.2 Publications

Gene expression databases

BgeeiP97484.
GenevisibleiP97484. MM.

Interactioni

Subunit structurei

Interacts with LYN, PTPN6/SHP-1 and PTPN11/SHP-2.3 Publications

Protein-protein interaction databases

DIPiDIP-59889N.
STRINGi10090.ENSMUSP00000077546.

Structurei

3D structure databases

ProteinModelPortaliP97484.
SMRiP97484. Positions 26-618.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 11690Ig-like C2-type 1Add
BLAST
Domaini118 – 220103Ig-like C2-type 2Add
BLAST
Domaini239 – 29759Ig-like C2-type 3Add
BLAST
Domaini308 – 428121Ig-like C2-type 4Add
BLAST
Domaini424 – 51390Ig-like C2-type 5Add
BLAST
Domaini537 – 61882Ig-like C2-type 6Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi711 – 7166ITIM motif 1By similarity
Motifi792 – 7976ITIM motif 2By similarity
Motifi822 – 8276ITIM motif 3By similarity

Domaini

Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, including PTPN6/SHP-1, resulting in the dephosphorylation of the downstream protein kinases SYK and BTK.1 Publication

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IKJD. Eukaryota.
ENOG41116BR. LUCA.
GeneTreeiENSGT00760000119033.
HOVERGENiHBG074353.
InParanoidiP97484.
KOiK06512.
OMAiCESWHND.
OrthoDBiEOG7R56S3.
PhylomeDBiP97484.
TreeFamiTF336644.

Family and domain databases

Gene3Di2.60.40.10. 6 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF13895. Ig_2. 4 hits.
[Graphical view]
SMARTiSM00409. IG. 5 hits.
SM00408. IGc2. 4 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 6 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97484-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCTFTALLR LGLTLSLWIP VLTGSLPKPI LRVQPDSVVS RRTKVTFLCE
60 70 80 90 100
ETIGANEYRL YKDGKLYKTV TKNKQKPENK AEFSFSNVDL SNAGQYRCSY
110 120 130 140 150
STQYKSSGYS DLLELVVTGH YWTPSLLAQA SPVVTSGGYV TLQCESWHND
160 170 180 190 200
HKFILTVEGP QKLSWTQDSQ YNYSTRKYHA LFSVGPVTPN QRWICRCYSY
210 220 230 240 250
DRNRPYVWSP PSESVELLVS GNLQKPTIKA EPGSVITSKR AMTIWCQGNL
260 270 280 290 300
DAEVYFLHNE KSQKTQSTQT LQEPGNKGKF FIPSVTLQHA GQYRCYCYGS
310 320 330 340 350
AGWSQPSDTL ELVVTGIYEY YEPRLSVLPS PVVTAGGNMT LHCASDFPYD
360 370 380 390 400
KFILTKEDKK FGNSLDTEHI SSSGQYRALF IIGPTTPTHT GAFRCYGYYK
410 420 430 440 450
NAPQLWSVPS ALQQILISGL SKKPSLLTHQ GHILDPGMTL TLQCFSDINY
460 470 480 490 500
DRFALHKVGG ADIMQHSSQQ TDTGFSVANF TLGYVSSSTG GQYRCYGAHN
510 520 530 540 550
LSSEWSASSE PLDILITGQL PLTPSLSVQP NHTVHSGETV SLLCWSMDSV
560 570 580 590 600
DTFILSKEGS AQQPLRLKSK SHDQQSQAEF SMSAVTSHLS GTYRCYGAQD
610 620 630 640 650
SSFYLLSSAS APVELTVSGP IETSTPPPTM SMPLGGLHMY LKALIGVSVA
660 670 680 690 700
FILFLFIFIF ILLRRRHRGK FRKDVQKEKD LQLSSGAEEP ITRKGELQKR
710 720 730 740 750
PNPAAATQEE SLYASVEDMQ TEDGVELNSW TPPEEDPQGE TYAQVKPSRL
760 770 780 790 800
RKAGHVSPSV MSREQLNTEY EQAEEGQGAN NQAAESGESQ DVTYAQLCSR
810 820 830 840
TLRQGAAASP LSQAGEAPEE PSVYATLAAA RPEAVPKDME Q
Length:841
Mass (Da):93,054
Last modified:May 1, 1997 - v1
Checksum:i33E9B3C51F44FF3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83172 mRNA. Translation: AAB40934.1.
AC130680 Genomic DNA. No translation available.
AC171680 Genomic DNA. No translation available.
CCDSiCCDS39730.1.
RefSeqiNP_035225.2. NM_011095.2.
UniGeneiMm.326531.
Mm.440814.

Genome annotation databases

EnsembliENSMUST00000078451; ENSMUSP00000077546; ENSMUSG00000058818.
GeneIDi18733.
KEGGimmu:18733.
UCSCiuc009ewb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83172 mRNA. Translation: AAB40934.1.
AC130680 Genomic DNA. No translation available.
AC171680 Genomic DNA. No translation available.
CCDSiCCDS39730.1.
RefSeqiNP_035225.2. NM_011095.2.
UniGeneiMm.326531.
Mm.440814.

3D structure databases

ProteinModelPortaliP97484.
SMRiP97484. Positions 26-618.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59889N.
STRINGi10090.ENSMUSP00000077546.

PTM databases

iPTMnetiP97484.
PhosphoSiteiP97484.

Proteomic databases

MaxQBiP97484.
PaxDbiP97484.
PRIDEiP97484.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078451; ENSMUSP00000077546; ENSMUSG00000058818.
GeneIDi18733.
KEGGimmu:18733.
UCSCiuc009ewb.2. mouse.

Organism-specific databases

CTDi18733.
MGIiMGI:894311. Lilrb3.

Phylogenomic databases

eggNOGiENOG410IKJD. Eukaryota.
ENOG41116BR. LUCA.
GeneTreeiENSGT00760000119033.
HOVERGENiHBG074353.
InParanoidiP97484.
KOiK06512.
OMAiCESWHND.
OrthoDBiEOG7R56S3.
PhylomeDBiP97484.
TreeFamiTF336644.

Miscellaneous databases

PROiP97484.
SOURCEiSearch...

Gene expression databases

BgeeiP97484.
GenevisibleiP97484. MM.

Family and domain databases

Gene3Di2.60.40.10. 6 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF13895. Ig_2. 4 hits.
[Graphical view]
SMARTiSM00409. IG. 5 hits.
SM00408. IGc2. 4 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 6 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a novel murine cell-surface glycoprotein homologous to killer cell inhibitory receptors."
    Hayami K., Fukuta D., Nishikawa Y., Yamashita Y., Inui M., Ohyama Y., Hikida M., Ohmori H., Takai T.
    J. Biol. Chem. 272:7320-7327(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: B10.A.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The paired Ig-like receptor PIR-B is an inhibitory receptor that recruits the protein-tyrosine phosphatase SHP-1."
    Blery M., Kubagawa H., Chen C.C., Vely F., Cooper M.D., Vivier E.
    Proc. Natl. Acad. Sci. U.S.A. 95:2446-2451(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TYR-794 AND TYR-824, INTERACTION WITH PTPN6/SHP-1 AND PTPN11/SHP-2, SUBCELLULAR LOCATION.
  4. "Paired immunoglobulin-like receptor B (PIR-B) inhibits BCR-induced activation of Syk and Btk by SHP-1."
    Maeda A., Scharenberg A.M., Tsukada S., Bolen J.B., Kinet J.P., Kurosaki T.
    Oncogene 18:2291-2297(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT TYR-794 AND TYR-824 BY LYN, MUTAGENESIS OF TYR-794 AND TYR-824, INTERACTION WITH PTPN6/SHP-1 AND PTPN11/SHP-2, SUBCELLULAR LOCATION, DOMAIN.
  5. "Constitutive tyrosine phosphorylation of the inhibitory paired Ig-like receptor PIR-B."
    Ho L.H., Uehara T., Chen C.C., Kubagawa H., Cooper M.D.
    Proc. Natl. Acad. Sci. U.S.A. 96:15086-15090(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION BY LYN, INTERACTION WITH LYN AND PTPN6/SHP-1, TISSUE SPECIFICITY.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-684 AND SER-757, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLIRB3_MOUSE
AccessioniPrimary (citable) accession number: P97484
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: May 1, 1997
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Belongs to the leukocyte receptor cluster (LRC) present on chromosome 7.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.