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P97473

- TRBP2_MOUSE

UniProt

P97473 - TRBP2_MOUSE

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Protein

RISC-loading complex subunit TARBP2

Gene
Tarbp2, Prbp
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1 By similarity. Binds in vitro to the PRM1 3'-UTR. Seems to act as a repressor of translation.1 Publication

GO - Molecular functioni

  1. double-stranded RNA binding Source: InterPro
  2. miRNA binding Source: UniProtKB-HAMAP
  3. protein binding Source: UniProtKB
  4. siRNA binding Source: UniProtKB

GO - Biological processi

  1. miRNA loading onto RISC involved in gene silencing by miRNA Source: UniProtKB-HAMAP
  2. negative regulation of defense response to virus by host Source: UniProtKB
  3. positive regulation of viral genome replication Source: UniProtKB
  4. pre-miRNA processing Source: UniProtKB
  5. production of siRNA involved in RNA interference Source: UniProtKB
  6. regulation of translation Source: UniProtKB-KW
  7. regulation of viral transcription Source: UniProtKB
  8. targeting of mRNA for destruction involved in RNA interference Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

RNA-mediated gene silencing, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiREACT_201975. Small interfering RNA (siRNA) biogenesis.
REACT_221178. MicroRNA (miRNA) biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
RISC-loading complex subunit TARBP2
Alternative name(s):
Protamine-1 RNA-binding protein
Short name:
PRM-1 RNA-binding protein
TAR RNA-binding protein 2
Gene namesi
Name:Tarbp2
Synonyms:Prbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:103027. Tarbp2.

Subcellular locationi

Cytoplasm. Cytoplasmperinuclear region By similarity. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB-SubCell
  3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  4. RISC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365RISC-loading complex subunit TARBP2UniRule annotationPRO_0000065623Add
BLAST

Proteomic databases

MaxQBiP97473.
PaxDbiP97473.
PRIDEiP97473.

PTM databases

PhosphoSiteiP97473.

Expressioni

Gene expression databases

ArrayExpressiP97473.
BgeeiP97473.
CleanExiMM_TARBP2.
GenevestigatoriP97473.

Interactioni

Subunit structurei

Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with EIF2AK2/PKR and inhibits its protein kinase activity. Interacts with DHX9, EIF6 and MOV10. Associates with the 60S ribosome By similarity. Interacts with DICER1 and PRKRA.2 Publications

Protein-protein interaction databases

IntActiP97473. 3 interactions.
STRINGi10090.ENSMUSP00000023813.

Structurei

3D structure databases

ProteinModelPortaliP97473.
SMRiP97473. Positions 28-224, 290-358.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 9768DRBM 1Add
BLAST
Domaini158 – 22669DRBM 2Add
BLAST
Domaini292 – 36069DRBM 3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 10584Sufficient for interaction with PRKRA By similarityAdd
BLAST
Regioni151 – 23383Sufficient for interaction with PRKRA By similarityAdd
BLAST
Regioni227 – 365139Sufficient for interaction with DICER1 By similarityAdd
BLAST
Regioni286 – 36580Sufficient for interaction with PRKRA By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the TARBP2 family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG245126.
GeneTreeiENSGT00730000110945.
HOGENOMiHOG000231919.
HOVERGENiHBG001700.
InParanoidiQ99M41.
OMAiIEQMLAV.
TreeFamiTF315953.

Family and domain databases

Gene3Di3.30.160.20. 3 hits.
HAMAPiMF_03034. TRBP2.
InterProiIPR014720. dsRNA-bd_dom.
IPR028605. TRBP2.
[Graphical view]
PANTHERiPTHR10910:SF61. PTHR10910:SF61. 1 hit.
PfamiPF00035. dsrm. 2 hits.
[Graphical view]
SMARTiSM00358. DSRM. 3 hits.
[Graphical view]
PROSITEiPS50137. DS_RBD. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97473-1 [UniParc]FASTAAdd to Basket

« Hide

MSEEDQGSGT TTGCGLPSIE QMLAANPGKT PISLLQEYGT RIGKTPVYDL    50
LKAEGQAHQP NFTFRVTVGD TSCTGQGPSK KAAKHKAAEV ALKHLKGGSM 100
LEPALEDSSS FSLLDSSPPE DTPVVAAEAA APVPSAVLTR SPPMEMQPPV 150
SPQQSECNPV GALQELVVQK GWRLPEYMVT QESGPAHRKE FTMTCRVERF 200
IEIGSGTSKK LAKRNAAAKM LLRVHTVPLD ARDGNEAEPD DDHFSIGVSS 250
RLDGLRNRGP GCTWDSLRNS VGEKILSLRS CSVGSLGALG SACCSVLSEL 300
SEEQAFHVSY LDIEELSLSG LCQCLVELST QPATVCYGSA TTREAARGDA 350
AHRALQYLRI MAGSK 365
Length:365
Mass (Da):38,842
Last modified:July 27, 2011 - v2
Checksum:i220A1D10D71CC249
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531A → E in AAB38885. 1 Publication
Sequence conflicti76 – 761Q → T in AAB38885. 1 Publication
Sequence conflicti111 – 1111F → L in AAB38885. 1 Publication
Sequence conflicti258 – 2581R → H in AAB38885. 1 Publication
Sequence conflicti334 – 3341T → A in AAB38885. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U79962 mRNA. Translation: AAB38885.1.
CH466550 Genomic DNA. Translation: EDL03967.1.
BC002028 mRNA. Translation: AAH02028.1.
CCDSiCCDS27886.1.
RefSeqiNP_033345.2. NM_009319.3.
UniGeneiMm.291485.

Genome annotation databases

EnsembliENSMUST00000023813; ENSMUSP00000023813; ENSMUSG00000023051.
GeneIDi21357.
KEGGimmu:21357.
UCSCiuc007xwf.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U79962 mRNA. Translation: AAB38885.1 .
CH466550 Genomic DNA. Translation: EDL03967.1 .
BC002028 mRNA. Translation: AAH02028.1 .
CCDSi CCDS27886.1.
RefSeqi NP_033345.2. NM_009319.3.
UniGenei Mm.291485.

3D structure databases

ProteinModelPortali P97473.
SMRi P97473. Positions 28-224, 290-358.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P97473. 3 interactions.
STRINGi 10090.ENSMUSP00000023813.

PTM databases

PhosphoSitei P97473.

Proteomic databases

MaxQBi P97473.
PaxDbi P97473.
PRIDEi P97473.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023813 ; ENSMUSP00000023813 ; ENSMUSG00000023051 .
GeneIDi 21357.
KEGGi mmu:21357.
UCSCi uc007xwf.3. mouse.

Organism-specific databases

CTDi 6895.
MGIi MGI:103027. Tarbp2.

Phylogenomic databases

eggNOGi NOG245126.
GeneTreei ENSGT00730000110945.
HOGENOMi HOG000231919.
HOVERGENi HBG001700.
InParanoidi Q99M41.
OMAi IEQMLAV.
TreeFami TF315953.

Enzyme and pathway databases

Reactomei REACT_201975. Small interfering RNA (siRNA) biogenesis.
REACT_221178. MicroRNA (miRNA) biogenesis.

Miscellaneous databases

NextBioi 300566.
PROi P97473.
SOURCEi Search...

Gene expression databases

ArrayExpressi P97473.
Bgeei P97473.
CleanExi MM_TARBP2.
Genevestigatori P97473.

Family and domain databases

Gene3Di 3.30.160.20. 3 hits.
HAMAPi MF_03034. TRBP2.
InterProi IPR014720. dsRNA-bd_dom.
IPR028605. TRBP2.
[Graphical view ]
PANTHERi PTHR10910:SF61. PTHR10910:SF61. 1 hit.
Pfami PF00035. dsrm. 2 hits.
[Graphical view ]
SMARTi SM00358. DSRM. 3 hits.
[Graphical view ]
PROSITEi PS50137. DS_RBD. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A testis cytoplasmic RNA-binding protein that has the properties of a translational repressor."
    Lee K., Fajardo M.A., Braun R.E.
    Mol. Cell. Biol. 16:3023-3034(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Testis.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing."
    Haase A.D., Jaskiewicz L., Zhang H., Laine S., Sack R., Gatignol A., Filipowicz W.
    EMBO Rep. 6:961-967(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DICER1.
  5. "Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA."
    Kok K.H., Ng M.-H., Ching Y.-P., Jin D.-Y.
    J. Biol. Chem. 282:17649-17657(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DICER1 AND PRKRA.

Entry informationi

Entry nameiTRBP2_MOUSE
AccessioniPrimary (citable) accession number: P97473
Secondary accession number(s): Q99M41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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