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P97473 (TRBP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RISC-loading complex subunit TARBP2
Alternative name(s):
Protamine-1 RNA-binding protein
Short name=PRM-1 RNA-binding protein
TAR RNA-binding protein 2
Gene names
Name:Tarbp2
Synonyms:Prbp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1 By similarity. Binds in vitro to the PRM1 3'-UTR. Seems to act as a repressor of translation. Ref.1

Subunit structure

Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with EIF2AK2/PKR and inhibits its protein kinase activity. Interacts with DHX9, EIF6 and MOV10. Associates with the 60S ribosome By similarity. Interacts with DICER1 and PRKRA. Ref.4 Ref.5

Subcellular location

Cytoplasm. Cytoplasmperinuclear region By similarity. Nucleus Ref.1.

Sequence similarities

Belongs to the TARBP2 family.

Contains 3 DRBM (double-stranded RNA-binding) domains.

Ontologies

Keywords
   Biological processRNA-mediated gene silencing
Translation regulation
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
   LigandRNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmiRNA loading onto RISC involved in gene silencing by miRNA

Inferred from electronic annotation. Source: UniProtKB-HAMAP

negative regulation of defense response to virus by host

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of viral genome replication

Inferred from sequence or structural similarity. Source: UniProtKB

pre-miRNA processing

Inferred from sequence or structural similarity. Source: UniProtKB

production of siRNA involved in RNA interference

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of translation

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of viral transcription

Inferred from sequence or structural similarity. Source: UniProtKB

targeting of mRNA for destruction involved in RNA interference

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentRISC complex

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondouble-stranded RNA binding

Inferred from electronic annotation. Source: InterPro

miRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

siRNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365RISC-loading complex subunit TARBP2 HAMAP-Rule MF_03034
PRO_0000065623

Regions

Domain30 – 9768DRBM 1
Domain158 – 22669DRBM 2
Domain292 – 36069DRBM 3
Region22 – 10584Sufficient for interaction with PRKRA By similarity
Region151 – 23383Sufficient for interaction with PRKRA By similarity
Region227 – 365139Sufficient for interaction with DICER1 By similarity
Region286 – 36580Sufficient for interaction with PRKRA By similarity

Experimental info

Sequence conflict531A → E in AAB38885. Ref.1
Sequence conflict761Q → T in AAB38885. Ref.1
Sequence conflict1111F → L in AAB38885. Ref.1
Sequence conflict2581R → H in AAB38885. Ref.1
Sequence conflict3341T → A in AAB38885. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P97473 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 220A1D10D71CC249

FASTA36538,842
        10         20         30         40         50         60 
MSEEDQGSGT TTGCGLPSIE QMLAANPGKT PISLLQEYGT RIGKTPVYDL LKAEGQAHQP 

        70         80         90        100        110        120 
NFTFRVTVGD TSCTGQGPSK KAAKHKAAEV ALKHLKGGSM LEPALEDSSS FSLLDSSPPE 

       130        140        150        160        170        180 
DTPVVAAEAA APVPSAVLTR SPPMEMQPPV SPQQSECNPV GALQELVVQK GWRLPEYMVT 

       190        200        210        220        230        240 
QESGPAHRKE FTMTCRVERF IEIGSGTSKK LAKRNAAAKM LLRVHTVPLD ARDGNEAEPD 

       250        260        270        280        290        300 
DDHFSIGVSS RLDGLRNRGP GCTWDSLRNS VGEKILSLRS CSVGSLGALG SACCSVLSEL 

       310        320        330        340        350        360 
SEEQAFHVSY LDIEELSLSG LCQCLVELST QPATVCYGSA TTREAARGDA AHRALQYLRI 


MAGSK 

« Hide

References

« Hide 'large scale' references
[1]"A testis cytoplasmic RNA-binding protein that has the properties of a translational repressor."
Lee K., Fajardo M.A., Braun R.E.
Mol. Cell. Biol. 16:3023-3034(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
Tissue: Testis.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[4]"TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing."
Haase A.D., Jaskiewicz L., Zhang H., Laine S., Sack R., Gatignol A., Filipowicz W.
EMBO Rep. 6:961-967(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DICER1.
[5]"Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA."
Kok K.H., Ng M.-H., Ching Y.-P., Jin D.-Y.
J. Biol. Chem. 282:17649-17657(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DICER1 AND PRKRA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U79962 mRNA. Translation: AAB38885.1.
CH466550 Genomic DNA. Translation: EDL03967.1.
BC002028 mRNA. Translation: AAH02028.1.
RefSeqNP_033345.2. NM_009319.3.
UniGeneMm.291485.

3D structure databases

ProteinModelPortalP97473.
SMRP97473. Positions 28-230, 292-359.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP97473. 3 interactions.
STRING10090.ENSMUSP00000023813.

PTM databases

PhosphoSiteP97473.

Proteomic databases

PaxDbP97473.
PRIDEP97473.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023813; ENSMUSP00000023813; ENSMUSG00000023051.
GeneID21357.
KEGGmmu:21357.
UCSCuc007xwf.3. mouse.

Organism-specific databases

CTD6895.
MGIMGI:103027. Tarbp2.

Phylogenomic databases

eggNOGNOG245126.
GeneTreeENSGT00730000110945.
HOGENOMHOG000231919.
HOVERGENHBG001700.
InParanoidQ99M41.
OMAIEQMLAV.
TreeFamTF315953.

Gene expression databases

ArrayExpressP97473.
BgeeP97473.
CleanExMM_TARBP2.
GenevestigatorP97473.

Family and domain databases

Gene3D3.30.160.20. 3 hits.
HAMAPMF_03034. TRBP2.
InterProIPR014720. dsRNA-bd_dom.
IPR028605. TRBP2.
[Graphical view]
PANTHERPTHR10910:SF61. PTHR10910:SF61. 1 hit.
PfamPF00035. dsrm. 2 hits.
[Graphical view]
SMARTSM00358. DSRM. 3 hits.
[Graphical view]
PROSITEPS50137. DS_RBD. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio300566.
PROP97473.
SOURCESearch...

Entry information

Entry nameTRBP2_MOUSE
AccessionPrimary (citable) accession number: P97473
Secondary accession number(s): Q99M41
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: March 19, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot