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Protein

RISC-loading complex subunit TARBP2

Gene

Tarbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1 (By similarity). Binds in vitro to the PRM1 3'-UTR. Seems to act as a repressor of translation.By similarity1 Publication

GO - Molecular functioni

  1. double-stranded RNA binding Source: InterPro
  2. miRNA binding Source: UniProtKB-HAMAP
  3. protein homodimerization activity Source: MGI
  4. siRNA binding Source: UniProtKB

GO - Biological processi

  1. miRNA loading onto RISC involved in gene silencing by miRNA Source: MGI
  2. multicellular organism growth Source: MGI
  3. negative regulation of defense response to virus by host Source: UniProtKB
  4. positive regulation of translation Source: MGI
  5. positive regulation of viral genome replication Source: UniProtKB
  6. pre-miRNA processing Source: UniProtKB
  7. production of siRNA involved in RNA interference Source: UniProtKB
  8. regulation of viral transcription Source: UniProtKB
  9. single fertilization Source: MGI
  10. spermatid development Source: MGI
  11. targeting of mRNA for destruction involved in RNA interference Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

RNA-mediated gene silencing, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiREACT_315548. MicroRNA (miRNA) biogenesis.
REACT_341029. Small interfering RNA (siRNA) biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
RISC-loading complex subunit TARBP2UniRule annotation
Alternative name(s):
Protamine-1 RNA-binding protein
Short name:
PRM-1 RNA-binding protein
TAR RNA-binding protein 2
Gene namesi
Name:Tarbp2
Synonyms:Prbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:103027. Tarbp2.

Subcellular locationi

  1. Cytoplasm UniRule annotation1 Publication
  2. Cytoplasmperinuclear region UniRule annotation
  3. Nucleus UniRule annotation1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. micro-ribonucleoprotein complex Source: MGI
  3. nucleoplasm Source: MGI
  4. nucleus Source: MGI
  5. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  6. RISC complex Source: UniProtKB
  7. RISC-loading complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365RISC-loading complex subunit TARBP2PRO_0000065623Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei151 – 1511PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP97473.
PaxDbiP97473.
PRIDEiP97473.

PTM databases

PhosphoSiteiP97473.

Expressioni

Gene expression databases

BgeeiP97473.
CleanExiMM_TARBP2.
ExpressionAtlasiP97473. baseline and differential.
GenevestigatoriP97473.

Interactioni

Subunit structurei

Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with EIF2AK2/PKR and inhibits its protein kinase activity. Interacts with DHX9, EIF6 and MOV10. Associates with the 60S ribosome (By similarity). Interacts with DICER1 and PRKRA.By similarity2 Publications

Protein-protein interaction databases

IntActiP97473. 3 interactions.
STRINGi10090.ENSMUSP00000023813.

Structurei

3D structure databases

ProteinModelPortaliP97473.
SMRiP97473. Positions 28-224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 9768DRBM 1UniRule annotationAdd
BLAST
Domaini158 – 22669DRBM 2UniRule annotationAdd
BLAST
Domaini292 – 36069DRBM 3UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 10584Sufficient for interaction with PRKRAUniRule annotationAdd
BLAST
Regioni151 – 23383Sufficient for interaction with PRKRAUniRule annotationAdd
BLAST
Regioni227 – 365139Sufficient for interaction with DICER1UniRule annotationAdd
BLAST
Regioni286 – 36580Sufficient for interaction with PRKRAUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TARBP2 family.UniRule annotation
Contains 3 DRBM (double-stranded RNA-binding) domains.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG245126.
GeneTreeiENSGT00760000119275.
HOGENOMiHOG000231919.
HOVERGENiHBG001700.
InParanoidiP97473.
KOiK18420.
OMAiQYLRIMA.
TreeFamiTF315953.

Family and domain databases

Gene3Di3.30.160.20. 3 hits.
HAMAPiMF_03034. TRBP2.
InterProiIPR014720. dsRNA-bd_dom.
IPR028605. TRBP2.
[Graphical view]
PANTHERiPTHR10910:SF61. PTHR10910:SF61. 1 hit.
PfamiPF00035. dsrm. 2 hits.
[Graphical view]
SMARTiSM00358. DSRM. 3 hits.
[Graphical view]
PROSITEiPS50137. DS_RBD. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEEDQGSGT TTGCGLPSIE QMLAANPGKT PISLLQEYGT RIGKTPVYDL
60 70 80 90 100
LKAEGQAHQP NFTFRVTVGD TSCTGQGPSK KAAKHKAAEV ALKHLKGGSM
110 120 130 140 150
LEPALEDSSS FSLLDSSPPE DTPVVAAEAA APVPSAVLTR SPPMEMQPPV
160 170 180 190 200
SPQQSECNPV GALQELVVQK GWRLPEYMVT QESGPAHRKE FTMTCRVERF
210 220 230 240 250
IEIGSGTSKK LAKRNAAAKM LLRVHTVPLD ARDGNEAEPD DDHFSIGVSS
260 270 280 290 300
RLDGLRNRGP GCTWDSLRNS VGEKILSLRS CSVGSLGALG SACCSVLSEL
310 320 330 340 350
SEEQAFHVSY LDIEELSLSG LCQCLVELST QPATVCYGSA TTREAARGDA
360
AHRALQYLRI MAGSK
Length:365
Mass (Da):38,842
Last modified:July 27, 2011 - v2
Checksum:i220A1D10D71CC249
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531A → E in AAB38885 (PubMed:8649414).Curated
Sequence conflicti76 – 761Q → T in AAB38885 (PubMed:8649414).Curated
Sequence conflicti111 – 1111F → L in AAB38885 (PubMed:8649414).Curated
Sequence conflicti258 – 2581R → H in AAB38885 (PubMed:8649414).Curated
Sequence conflicti334 – 3341T → A in AAB38885 (PubMed:8649414).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U79962 mRNA. Translation: AAB38885.1.
CH466550 Genomic DNA. Translation: EDL03967.1.
BC002028 mRNA. Translation: AAH02028.1.
CCDSiCCDS27886.1.
RefSeqiNP_033345.2. NM_009319.3.
UniGeneiMm.291485.

Genome annotation databases

EnsembliENSMUST00000023813; ENSMUSP00000023813; ENSMUSG00000023051.
GeneIDi21357.
KEGGimmu:21357.
UCSCiuc007xwf.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U79962 mRNA. Translation: AAB38885.1.
CH466550 Genomic DNA. Translation: EDL03967.1.
BC002028 mRNA. Translation: AAH02028.1.
CCDSiCCDS27886.1.
RefSeqiNP_033345.2. NM_009319.3.
UniGeneiMm.291485.

3D structure databases

ProteinModelPortaliP97473.
SMRiP97473. Positions 28-224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP97473. 3 interactions.
STRINGi10090.ENSMUSP00000023813.

PTM databases

PhosphoSiteiP97473.

Proteomic databases

MaxQBiP97473.
PaxDbiP97473.
PRIDEiP97473.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023813; ENSMUSP00000023813; ENSMUSG00000023051.
GeneIDi21357.
KEGGimmu:21357.
UCSCiuc007xwf.3. mouse.

Organism-specific databases

CTDi6895.
MGIiMGI:103027. Tarbp2.

Phylogenomic databases

eggNOGiNOG245126.
GeneTreeiENSGT00760000119275.
HOGENOMiHOG000231919.
HOVERGENiHBG001700.
InParanoidiP97473.
KOiK18420.
OMAiQYLRIMA.
TreeFamiTF315953.

Enzyme and pathway databases

ReactomeiREACT_315548. MicroRNA (miRNA) biogenesis.
REACT_341029. Small interfering RNA (siRNA) biogenesis.

Miscellaneous databases

NextBioi300566.
PROiP97473.
SOURCEiSearch...

Gene expression databases

BgeeiP97473.
CleanExiMM_TARBP2.
ExpressionAtlasiP97473. baseline and differential.
GenevestigatoriP97473.

Family and domain databases

Gene3Di3.30.160.20. 3 hits.
HAMAPiMF_03034. TRBP2.
InterProiIPR014720. dsRNA-bd_dom.
IPR028605. TRBP2.
[Graphical view]
PANTHERiPTHR10910:SF61. PTHR10910:SF61. 1 hit.
PfamiPF00035. dsrm. 2 hits.
[Graphical view]
SMARTiSM00358. DSRM. 3 hits.
[Graphical view]
PROSITEiPS50137. DS_RBD. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A testis cytoplasmic RNA-binding protein that has the properties of a translational repressor."
    Lee K., Fajardo M.A., Braun R.E.
    Mol. Cell. Biol. 16:3023-3034(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Testis.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing."
    Haase A.D., Jaskiewicz L., Zhang H., Laine S., Sack R., Gatignol A., Filipowicz W.
    EMBO Rep. 6:961-967(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DICER1.
  5. "Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA."
    Kok K.H., Ng M.-H., Ching Y.-P., Jin D.-Y.
    J. Biol. Chem. 282:17649-17657(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DICER1 AND PRKRA.

Entry informationi

Entry nameiTRBP2_MOUSE
AccessioniPrimary (citable) accession number: P97473
Secondary accession number(s): Q99M41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: April 29, 2015
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.