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P97473

- TRBP2_MOUSE

UniProt

P97473 - TRBP2_MOUSE

Protein

RISC-loading complex subunit TARBP2

Gene

Tarbp2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1 By similarity. Binds in vitro to the PRM1 3'-UTR. Seems to act as a repressor of translation.By similarity1 Publication

    GO - Molecular functioni

    1. double-stranded RNA binding Source: InterPro
    2. miRNA binding Source: UniProtKB-HAMAP
    3. protein binding Source: UniProtKB
    4. siRNA binding Source: UniProtKB

    GO - Biological processi

    1. miRNA loading onto RISC involved in gene silencing by miRNA Source: UniProtKB-HAMAP
    2. negative regulation of defense response to virus by host Source: UniProtKB
    3. positive regulation of viral genome replication Source: UniProtKB
    4. pre-miRNA processing Source: UniProtKB
    5. production of siRNA involved in RNA interference Source: UniProtKB
    6. regulation of translation Source: UniProtKB-KW
    7. regulation of viral transcription Source: UniProtKB
    8. targeting of mRNA for destruction involved in RNA interference Source: UniProtKB

    Keywords - Biological processi

    RNA-mediated gene silencing, Translation regulation

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_201975. Small interfering RNA (siRNA) biogenesis.
    REACT_221178. MicroRNA (miRNA) biogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RISC-loading complex subunit TARBP2UniRule annotation
    Alternative name(s):
    Protamine-1 RNA-binding protein
    Short name:
    PRM-1 RNA-binding protein
    TAR RNA-binding protein 2
    Gene namesi
    Name:Tarbp2
    Synonyms:Prbp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:103027. Tarbp2.

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation. Cytoplasmperinuclear region UniRule annotation. Nucleus 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB-SubCell
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    4. RISC complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 365365RISC-loading complex subunit TARBP2PRO_0000065623Add
    BLAST

    Proteomic databases

    MaxQBiP97473.
    PaxDbiP97473.
    PRIDEiP97473.

    PTM databases

    PhosphoSiteiP97473.

    Expressioni

    Gene expression databases

    ArrayExpressiP97473.
    BgeeiP97473.
    CleanExiMM_TARBP2.
    GenevestigatoriP97473.

    Interactioni

    Subunit structurei

    Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with EIF2AK2/PKR and inhibits its protein kinase activity. Interacts with DHX9, EIF6 and MOV10. Associates with the 60S ribosome By similarity. Interacts with DICER1 and PRKRA.By similarity2 Publications

    Protein-protein interaction databases

    IntActiP97473. 3 interactions.
    STRINGi10090.ENSMUSP00000023813.

    Structurei

    3D structure databases

    ProteinModelPortaliP97473.
    SMRiP97473. Positions 28-224, 290-358.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 9768DRBM 1UniRule annotationAdd
    BLAST
    Domaini158 – 22669DRBM 2UniRule annotationAdd
    BLAST
    Domaini292 – 36069DRBM 3UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni22 – 10584Sufficient for interaction with PRKRAUniRule annotationAdd
    BLAST
    Regioni151 – 23383Sufficient for interaction with PRKRAUniRule annotationAdd
    BLAST
    Regioni227 – 365139Sufficient for interaction with DICER1UniRule annotationAdd
    BLAST
    Regioni286 – 36580Sufficient for interaction with PRKRAUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TARBP2 family.UniRule annotation
    Contains 3 DRBM (double-stranded RNA-binding) domains.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG245126.
    GeneTreeiENSGT00730000110945.
    HOGENOMiHOG000231919.
    HOVERGENiHBG001700.
    InParanoidiQ99M41.
    OMAiIEQMLAV.
    TreeFamiTF315953.

    Family and domain databases

    Gene3Di3.30.160.20. 3 hits.
    HAMAPiMF_03034. TRBP2.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR028605. TRBP2.
    [Graphical view]
    PANTHERiPTHR10910:SF61. PTHR10910:SF61. 1 hit.
    PfamiPF00035. dsrm. 2 hits.
    [Graphical view]
    SMARTiSM00358. DSRM. 3 hits.
    [Graphical view]
    PROSITEiPS50137. DS_RBD. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P97473-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEEDQGSGT TTGCGLPSIE QMLAANPGKT PISLLQEYGT RIGKTPVYDL    50
    LKAEGQAHQP NFTFRVTVGD TSCTGQGPSK KAAKHKAAEV ALKHLKGGSM 100
    LEPALEDSSS FSLLDSSPPE DTPVVAAEAA APVPSAVLTR SPPMEMQPPV 150
    SPQQSECNPV GALQELVVQK GWRLPEYMVT QESGPAHRKE FTMTCRVERF 200
    IEIGSGTSKK LAKRNAAAKM LLRVHTVPLD ARDGNEAEPD DDHFSIGVSS 250
    RLDGLRNRGP GCTWDSLRNS VGEKILSLRS CSVGSLGALG SACCSVLSEL 300
    SEEQAFHVSY LDIEELSLSG LCQCLVELST QPATVCYGSA TTREAARGDA 350
    AHRALQYLRI MAGSK 365
    Length:365
    Mass (Da):38,842
    Last modified:July 27, 2011 - v2
    Checksum:i220A1D10D71CC249
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 531A → E in AAB38885. (PubMed:8649414)Curated
    Sequence conflicti76 – 761Q → T in AAB38885. (PubMed:8649414)Curated
    Sequence conflicti111 – 1111F → L in AAB38885. (PubMed:8649414)Curated
    Sequence conflicti258 – 2581R → H in AAB38885. (PubMed:8649414)Curated
    Sequence conflicti334 – 3341T → A in AAB38885. (PubMed:8649414)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U79962 mRNA. Translation: AAB38885.1.
    CH466550 Genomic DNA. Translation: EDL03967.1.
    BC002028 mRNA. Translation: AAH02028.1.
    CCDSiCCDS27886.1.
    RefSeqiNP_033345.2. NM_009319.3.
    UniGeneiMm.291485.

    Genome annotation databases

    EnsembliENSMUST00000023813; ENSMUSP00000023813; ENSMUSG00000023051.
    GeneIDi21357.
    KEGGimmu:21357.
    UCSCiuc007xwf.3. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U79962 mRNA. Translation: AAB38885.1 .
    CH466550 Genomic DNA. Translation: EDL03967.1 .
    BC002028 mRNA. Translation: AAH02028.1 .
    CCDSi CCDS27886.1.
    RefSeqi NP_033345.2. NM_009319.3.
    UniGenei Mm.291485.

    3D structure databases

    ProteinModelPortali P97473.
    SMRi P97473. Positions 28-224, 290-358.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P97473. 3 interactions.
    STRINGi 10090.ENSMUSP00000023813.

    PTM databases

    PhosphoSitei P97473.

    Proteomic databases

    MaxQBi P97473.
    PaxDbi P97473.
    PRIDEi P97473.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023813 ; ENSMUSP00000023813 ; ENSMUSG00000023051 .
    GeneIDi 21357.
    KEGGi mmu:21357.
    UCSCi uc007xwf.3. mouse.

    Organism-specific databases

    CTDi 6895.
    MGIi MGI:103027. Tarbp2.

    Phylogenomic databases

    eggNOGi NOG245126.
    GeneTreei ENSGT00730000110945.
    HOGENOMi HOG000231919.
    HOVERGENi HBG001700.
    InParanoidi Q99M41.
    OMAi IEQMLAV.
    TreeFami TF315953.

    Enzyme and pathway databases

    Reactomei REACT_201975. Small interfering RNA (siRNA) biogenesis.
    REACT_221178. MicroRNA (miRNA) biogenesis.

    Miscellaneous databases

    NextBioi 300566.
    PROi P97473.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97473.
    Bgeei P97473.
    CleanExi MM_TARBP2.
    Genevestigatori P97473.

    Family and domain databases

    Gene3Di 3.30.160.20. 3 hits.
    HAMAPi MF_03034. TRBP2.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR028605. TRBP2.
    [Graphical view ]
    PANTHERi PTHR10910:SF61. PTHR10910:SF61. 1 hit.
    Pfami PF00035. dsrm. 2 hits.
    [Graphical view ]
    SMARTi SM00358. DSRM. 3 hits.
    [Graphical view ]
    PROSITEi PS50137. DS_RBD. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A testis cytoplasmic RNA-binding protein that has the properties of a translational repressor."
      Lee K., Fajardo M.A., Braun R.E.
      Mol. Cell. Biol. 16:3023-3034(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
      Tissue: Testis.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    4. "TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing."
      Haase A.D., Jaskiewicz L., Zhang H., Laine S., Sack R., Gatignol A., Filipowicz W.
      EMBO Rep. 6:961-967(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DICER1.
    5. "Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA."
      Kok K.H., Ng M.-H., Ching Y.-P., Jin D.-Y.
      J. Biol. Chem. 282:17649-17657(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DICER1 AND PRKRA.

    Entry informationi

    Entry nameiTRBP2_MOUSE
    AccessioniPrimary (citable) accession number: P97473
    Secondary accession number(s): Q99M41
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3