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Protein

Serine/threonine-protein phosphatase 4 catalytic subunit

Gene

Ppp4c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair (By similarity). Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase. In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin (By similarity).By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Manganese 1By similarity1
Metal bindingi56Manganese 1By similarity1
Metal bindingi82Manganese 1By similarity1
Metal bindingi82Manganese 2By similarity1
Metal bindingi114Manganese 2By similarity1
Active sitei115Proton donorBy similarity1
Metal bindingi164Manganese 2By similarity1
Metal bindingi238Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • NF-kappaB-inducing kinase activity Source: UniProtKB
  • protein serine/threonine phosphatase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5693607 Processing of DNA double-strand break ends

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit (EC:3.1.3.16)
Short name:
PP4C
Short name:
Pp4
Alternative name(s):
Protein phosphatase X
Short name:
PP-X
Gene namesi
Name:Ppp4c
Synonyms:Ppp4, Ppx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1891763 Ppp4c

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000588842 – 307Serine/threonine-protein phosphatase 4 catalytic subunitAdd BLAST306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei307Leucine methyl esterBy similarity1

Post-translational modificationi

Methylation at the C-terminal Leu-307 is critical for interactions with regulatory subunits and functions in DNA repair.By similarity

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

EPDiP97470
PaxDbiP97470
PeptideAtlasiP97470
PRIDEiP97470

PTM databases

iPTMnetiP97470
PhosphoSitePlusiP97470

Expressioni

Gene expression databases

BgeeiENSMUSG00000030697
CleanExiMM_PPP4C
ExpressionAtlasiP97470 baseline and differential
GenevisibleiP97470 MM

Interactioni

Subunit structurei

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2. Interacts with REL, NFKB1/p50 and RELA. Interacts with SMN1 AND GEMIN4. Interacts with IRS4 (phosphorylated). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2. Interacts with HDAC3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi20796631 interactors.
IntActiP97470 32 interactors.
STRINGi10090.ENSMUSP00000032936

Structurei

3D structure databases

ProteinModelPortaliP97470
SMRiP97470
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0372 Eukaryota
COG0639 LUCA
GeneTreeiENSGT00550000074618
HOGENOMiHOG000172696
HOVERGENiHBG000216
InParanoidiP97470
KOiK15423
OMAiPDRMTLI
OrthoDBiEOG091G0B6S
PhylomeDBiP97470
TreeFamiTF105559

Family and domain databases

Gene3Di3.60.21.101 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR006186 Ser/Thr-sp_prot-phosphatase
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97470-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEISDLDRQ IEQLRRCELI KESEVKALCA KAREILVEES NVQRVDSPVT
60 70 80 90 100
VCGDIHGQFY DLKELFRVGG DVPETNYLFM GDFVDRGFYS VETFLLLLAL
110 120 130 140 150
KVRYPDRITL IRGNHESRQI TQVYGFYDEC LRKYGSVTVW RYCTEIFDYL
160 170 180 190 200
SLSAIIDGKI FCVHGGLSPS IQTLDQIRTI DRKQEVPHDG PMCDLLWSDP
210 220 230 240 250
EDTTGWGVSP RGAGYLFGSD VVAQFNAAND IDMICRAHQL VMEGYKWHFN
260 270 280 290 300
ETVLTVWSAP NYCYRCGNVA AILELDEHLQ KDFIIFEAAP QETRGIPSKK

PVADYFL
Length:307
Mass (Da):35,080
Last modified:March 1, 2004 - v2
Checksum:iD6FE470A5C6CBCAC
GO

Sequence cautioni

The sequence AAB38494 differs from that shown. Reason: Frameshift at positions 101 and 106.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95 – 96LL → FF in AAB38494 (PubMed:11707325).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF088911 mRNA Translation: AAC96297.1
AF378669 Genomic DNA Translation: AAL35110.1
BC001993 mRNA Translation: AAH01993.1
U79747 mRNA Translation: AAB38494.1 Frameshift.
CCDSiCCDS21844.1
RefSeqiNP_062648.1, NM_019674.3
UniGeneiMm.41998

Genome annotation databases

EnsembliENSMUST00000032936; ENSMUSP00000032936; ENSMUSG00000030697
ENSMUST00000206570; ENSMUSP00000146245; ENSMUSG00000030697
GeneIDi56420
KEGGimmu:56420
UCSCiuc009jss.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPP4C_MOUSE
AccessioniPrimary (citable) accession number: P97470
Secondary accession number(s): P33172
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: March 28, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome