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P97470 (PP4C_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit

Short name=PP4C
Short name=Pp4
EC=3.1.3.16
Alternative name(s):
Protein phosphatase X
Short name=PP-X
Gene names
Name:Ppp4c
Synonyms:Ppp4, Ppx
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair By similarity. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase. In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin By similarity. Ref.6

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Subunit structure

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2. Interacts with REL, NFKB1/p50 and RELA. Interacts with SMN1 AND GEMIN4. Interacts with IRS4 (phosphorylated). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2. Interacts with HDAC3 By similarity. Ref.5

Subcellular location

Cytoplasm. Nucleus. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome.

Sequence similarities

Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.

Sequence caution

The sequence AAB38494.1 differs from that shown. Reason: Frameshift at positions 101 and 106.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 307306Serine/threonine-protein phosphatase 4 catalytic subunit
PRO_0000058884

Sites

Active site1151Proton donor By similarity
Metal binding541Iron By similarity
Metal binding561Iron By similarity
Metal binding821Iron By similarity
Metal binding821Manganese By similarity
Metal binding1141Manganese By similarity
Metal binding1641Manganese By similarity
Metal binding2381Manganese By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue3071Leucine methyl ester By similarity

Experimental info

Sequence conflict95 – 962LL → FF in AAB38494. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P97470 [UniParc].

Last modified March 1, 2004. Version 2.
Checksum: D6FE470A5C6CBCAC

FASTA30735,080
        10         20         30         40         50         60 
MAEISDLDRQ IEQLRRCELI KESEVKALCA KAREILVEES NVQRVDSPVT VCGDIHGQFY 

        70         80         90        100        110        120 
DLKELFRVGG DVPETNYLFM GDFVDRGFYS VETFLLLLAL KVRYPDRITL IRGNHESRQI 

       130        140        150        160        170        180 
TQVYGFYDEC LRKYGSVTVW RYCTEIFDYL SLSAIIDGKI FCVHGGLSPS IQTLDQIRTI 

       190        200        210        220        230        240 
DRKQEVPHDG PMCDLLWSDP EDTTGWGVSP RGAGYLFGSD VVAQFNAAND IDMICRAHQL 

       250        260        270        280        290        300 
VMEGYKWHFN ETVLTVWSAP NYCYRCGNVA AILELDEHLQ KDFIIFEAAP QETRGIPSKK 


PVADYFL 

« Hide

References

« Hide 'large scale' references
[1]"Protein phosphatase X interacts with c-Rel and stimulates c-Rel/nuclear factor kappaB activity."
Hu M.C.-T., Tang-Oxley Q., Qiu W.R., Wang Y.-P., Mihindukulasuriya K.A., Afshar R., Tan T.-H.
J. Biol. Chem. 273:33561-33565(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Genomic structure of the mouse PP4 gene: a developmentally regulated protein phosphatase."
Hu M.C.-T., Shui J.W., Mihindukulasuriya K.A., Tan T.-H.
Gene 278:89-99(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary tumor.
[4]Sussman D.J.
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 79-293.
Strain: BALB/c.
[5]"PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4."
Chen G.I., Tisayakorn S., Jorgensen C., D'Ambrosio L.M., Goudreault M., Gingras A.-C.
J. Biol. Chem. 283:29273-29284(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PPP4C.
[6]"Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation."
Toyo-oka K., Mori D., Yano Y., Shiota M., Iwao H., Goto H., Inagaki M., Hiraiwa N., Muramatsu M., Wynshaw-Boris A., Yoshiki A., Hirotsune S.
J. Cell Biol. 180:1133-1147(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF088911 mRNA. Translation: AAC96297.1.
AF378669 Genomic DNA. Translation: AAL35110.1.
BC001993 mRNA. Translation: AAH01993.1.
U79747 mRNA. Translation: AAB38494.1. Frameshift.
RefSeqNP_062648.1. NM_019674.3.
UniGeneMm.41998.

3D structure databases

ProteinModelPortalP97470.
SMRP97470. Positions 6-291.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207966. 1 interaction.
IntActP97470. 1 interaction.
STRING10090.ENSMUSP00000032936.

PTM databases

PhosphoSiteP97470.

Proteomic databases

PaxDbP97470.
PRIDEP97470.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032936; ENSMUSP00000032936; ENSMUSG00000030697.
GeneID56420.
KEGGmmu:56420.
UCSCuc009jss.1. mouse.

Organism-specific databases

CTD5531.
MGIMGI:1891763. Ppp4c.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172696.
HOVERGENHBG000216.
InParanoidP97470.
KOK15423.
OMAVFNHRND.
OrthoDBEOG74N5H2.
PhylomeDBP97470.
TreeFamTF105559.

Gene expression databases

ArrayExpressP97470.
BgeeP97470.
CleanExMM_PPP4C.
GenevestigatorP97470.

Family and domain databases

InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPPP4C. mouse.
NextBio21426.
PROP97470.
SOURCESearch...

Entry information

Entry namePP4C_MOUSE
AccessionPrimary (citable) accession number: P97470
Secondary accession number(s): P33172
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: April 16, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot