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P97470

- PP4C_MOUSE

UniProt

P97470 - PP4C_MOUSE

Protein

Serine/threonine-protein phosphatase 4 catalytic subunit

Gene

Ppp4c

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (01 Mar 2004)
      Previous versions | rss
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    Functioni

    Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair By similarity. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase. In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin By similarity.By similarity

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi54 – 541Manganese 1By similarity
    Metal bindingi56 – 561Manganese 1By similarity
    Metal bindingi82 – 821Manganese 1By similarity
    Metal bindingi82 – 821Manganese 2By similarity
    Metal bindingi114 – 1141Manganese 2By similarity
    Active sitei115 – 1151Proton donorBy similarity
    Metal bindingi164 – 1641Manganese 2By similarity
    Metal bindingi238 – 2381Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. NF-kappaB-inducing kinase activity Source: UniProtKB
    3. protein binding Source: MGI
    4. protein serine/threonine phosphatase activity Source: UniProtKB

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. NIK/NF-kappaB signaling Source: GOC
    3. regulation of double-strand break repair via homologous recombination Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 4 catalytic subunit (EC:3.1.3.16)
    Short name:
    PP4C
    Short name:
    Pp4
    Alternative name(s):
    Protein phosphatase X
    Short name:
    PP-X
    Gene namesi
    Name:Ppp4c
    Synonyms:Ppp4, Ppx
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1891763. Ppp4c.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. microtubule organizing center Source: UniProtKB-SubCell
    3. nucleus Source: MGI
    4. plasma membrane Source: Ensembl
    5. protein phosphatase 4 complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 307306Serine/threonine-protein phosphatase 4 catalytic subunitPRO_0000058884Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei307 – 3071Leucine methyl esterBy similarity

    Keywords - PTMi

    Acetylation, Methylation

    Proteomic databases

    MaxQBiP97470.
    PaxDbiP97470.
    PRIDEiP97470.

    PTM databases

    PhosphoSiteiP97470.

    Expressioni

    Gene expression databases

    ArrayExpressiP97470.
    BgeeiP97470.
    CleanExiMM_PPP4C.
    GenevestigatoriP97470.

    Interactioni

    Subunit structurei

    Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2. Interacts with REL, NFKB1/p50 and RELA. Interacts with SMN1 AND GEMIN4. Interacts with IRS4 (phosphorylated). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2. Interacts with HDAC3 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi207966. 1 interaction.
    IntActiP97470. 1 interaction.
    STRINGi10090.ENSMUSP00000032936.

    Structurei

    3D structure databases

    ProteinModelPortaliP97470.
    SMRiP97470. Positions 6-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172696.
    HOVERGENiHBG000216.
    InParanoidiP97470.
    KOiK15423.
    OMAiVFNHRND.
    OrthoDBiEOG74N5H2.
    PhylomeDBiP97470.
    TreeFamiTF105559.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97470-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEISDLDRQ IEQLRRCELI KESEVKALCA KAREILVEES NVQRVDSPVT    50
    VCGDIHGQFY DLKELFRVGG DVPETNYLFM GDFVDRGFYS VETFLLLLAL 100
    KVRYPDRITL IRGNHESRQI TQVYGFYDEC LRKYGSVTVW RYCTEIFDYL 150
    SLSAIIDGKI FCVHGGLSPS IQTLDQIRTI DRKQEVPHDG PMCDLLWSDP 200
    EDTTGWGVSP RGAGYLFGSD VVAQFNAAND IDMICRAHQL VMEGYKWHFN 250
    ETVLTVWSAP NYCYRCGNVA AILELDEHLQ KDFIIFEAAP QETRGIPSKK 300
    PVADYFL 307
    Length:307
    Mass (Da):35,080
    Last modified:March 1, 2004 - v2
    Checksum:iD6FE470A5C6CBCAC
    GO

    Sequence cautioni

    The sequence AAB38494.1 differs from that shown. Reason: Frameshift at positions 101 and 106.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti95 – 962LL → FF in AAB38494. (PubMed:11707325)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF088911 mRNA. Translation: AAC96297.1.
    AF378669 Genomic DNA. Translation: AAL35110.1.
    BC001993 mRNA. Translation: AAH01993.1.
    U79747 mRNA. Translation: AAB38494.1. Frameshift.
    CCDSiCCDS21844.1.
    RefSeqiNP_062648.1. NM_019674.3.
    UniGeneiMm.41998.

    Genome annotation databases

    EnsembliENSMUST00000032936; ENSMUSP00000032936; ENSMUSG00000030697.
    GeneIDi56420.
    KEGGimmu:56420.
    UCSCiuc009jss.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF088911 mRNA. Translation: AAC96297.1 .
    AF378669 Genomic DNA. Translation: AAL35110.1 .
    BC001993 mRNA. Translation: AAH01993.1 .
    U79747 mRNA. Translation: AAB38494.1 . Frameshift.
    CCDSi CCDS21844.1.
    RefSeqi NP_062648.1. NM_019674.3.
    UniGenei Mm.41998.

    3D structure databases

    ProteinModelPortali P97470.
    SMRi P97470. Positions 6-291.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207966. 1 interaction.
    IntActi P97470. 1 interaction.
    STRINGi 10090.ENSMUSP00000032936.

    PTM databases

    PhosphoSitei P97470.

    Proteomic databases

    MaxQBi P97470.
    PaxDbi P97470.
    PRIDEi P97470.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032936 ; ENSMUSP00000032936 ; ENSMUSG00000030697 .
    GeneIDi 56420.
    KEGGi mmu:56420.
    UCSCi uc009jss.1. mouse.

    Organism-specific databases

    CTDi 5531.
    MGIi MGI:1891763. Ppp4c.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172696.
    HOVERGENi HBG000216.
    InParanoidi P97470.
    KOi K15423.
    OMAi VFNHRND.
    OrthoDBi EOG74N5H2.
    PhylomeDBi P97470.
    TreeFami TF105559.

    Miscellaneous databases

    ChiTaRSi PPP4C. mouse.
    NextBioi 21426.
    PROi P97470.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97470.
    Bgeei P97470.
    CleanExi MM_PPP4C.
    Genevestigatori P97470.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Protein phosphatase X interacts with c-Rel and stimulates c-Rel/nuclear factor kappaB activity."
      Hu M.C.-T., Tang-Oxley Q., Qiu W.R., Wang Y.-P., Mihindukulasuriya K.A., Afshar R., Tan T.-H.
      J. Biol. Chem. 273:33561-33565(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Genomic structure of the mouse PP4 gene: a developmentally regulated protein phosphatase."
      Hu M.C.-T., Shui J.W., Mihindukulasuriya K.A., Tan T.-H.
      Gene 278:89-99(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    4. Sussman D.J.
      Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 79-293.
      Strain: BALB/c.
    5. "PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4."
      Chen G.I., Tisayakorn S., Jorgensen C., D'Ambrosio L.M., Goudreault M., Gingras A.-C.
      J. Biol. Chem. 283:29273-29284(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PPP4C.
    6. "Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation."
      Toyo-oka K., Mori D., Yano Y., Shiota M., Iwao H., Goto H., Inagaki M., Hiraiwa N., Muramatsu M., Wynshaw-Boris A., Yoshiki A., Hirotsune S.
      J. Cell Biol. 180:1133-1147(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPP4C_MOUSE
    AccessioniPrimary (citable) accession number: P97470
    Secondary accession number(s): P33172
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: March 1, 2004
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3