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Protein

Serine/threonine-protein phosphatase 4 catalytic subunit

Gene

Ppp4c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair (By similarity). Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase. In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Manganese 1By similarity
Metal bindingi56 – 561Manganese 1By similarity
Metal bindingi82 – 821Manganese 1By similarity
Metal bindingi82 – 821Manganese 2By similarity
Metal bindingi114 – 1141Manganese 2By similarity
Active sitei115 – 1151Proton donorBy similarity
Metal bindingi164 – 1641Manganese 2By similarity
Metal bindingi238 – 2381Manganese 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit (EC:3.1.3.16)
Short name:
PP4C
Short name:
Pp4
Alternative name(s):
Protein phosphatase X
Short name:
PP-X
Gene namesi
Name:Ppp4c
Synonyms:Ppp4, Ppx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1891763. Ppp4c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • microtubule organizing center Source: UniProtKB-SubCell
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: MGI
  • protein phosphatase 4 complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 307306Serine/threonine-protein phosphatase 4 catalytic subunitPRO_0000058884Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei307 – 3071Leucine methyl esterBy similarity

Post-translational modificationi

Methylation at the C-terminal Leu-307 is critical for interactions with regulatory subunits and functions in DNA repair.By similarity

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

MaxQBiP97470.
PaxDbiP97470.
PRIDEiP97470.

PTM databases

PhosphoSiteiP97470.

Expressioni

Gene expression databases

BgeeiP97470.
CleanExiMM_PPP4C.
ExpressionAtlasiP97470. baseline and differential.
GenevisibleiP97470. MM.

Interactioni

Subunit structurei

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2. Interacts with REL, NFKB1/p50 and RELA. Interacts with SMN1 AND GEMIN4. Interacts with IRS4 (phosphorylated). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2. Interacts with HDAC3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi207966. 1 interaction.
IntActiP97470. 1 interaction.
STRINGi10090.ENSMUSP00000032936.

Structurei

3D structure databases

ProteinModelPortaliP97470.
SMRiP97470. Positions 6-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00550000074618.
HOGENOMiHOG000172696.
HOVERGENiHBG000216.
InParanoidiP97470.
KOiK15423.
OMAiVFNHRND.
OrthoDBiEOG74N5H2.
PhylomeDBiP97470.
TreeFamiTF105559.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97470-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEISDLDRQ IEQLRRCELI KESEVKALCA KAREILVEES NVQRVDSPVT
60 70 80 90 100
VCGDIHGQFY DLKELFRVGG DVPETNYLFM GDFVDRGFYS VETFLLLLAL
110 120 130 140 150
KVRYPDRITL IRGNHESRQI TQVYGFYDEC LRKYGSVTVW RYCTEIFDYL
160 170 180 190 200
SLSAIIDGKI FCVHGGLSPS IQTLDQIRTI DRKQEVPHDG PMCDLLWSDP
210 220 230 240 250
EDTTGWGVSP RGAGYLFGSD VVAQFNAAND IDMICRAHQL VMEGYKWHFN
260 270 280 290 300
ETVLTVWSAP NYCYRCGNVA AILELDEHLQ KDFIIFEAAP QETRGIPSKK

PVADYFL
Length:307
Mass (Da):35,080
Last modified:March 1, 2004 - v2
Checksum:iD6FE470A5C6CBCAC
GO

Sequence cautioni

The sequence AAB38494.1 differs from that shown. Reason: Frameshift at positions 101 and 106. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 962LL → FF in AAB38494 (PubMed:11707325).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF088911 mRNA. Translation: AAC96297.1.
AF378669 Genomic DNA. Translation: AAL35110.1.
BC001993 mRNA. Translation: AAH01993.1.
U79747 mRNA. Translation: AAB38494.1. Frameshift.
CCDSiCCDS21844.1.
RefSeqiNP_062648.1. NM_019674.3.
UniGeneiMm.41998.

Genome annotation databases

EnsembliENSMUST00000032936; ENSMUSP00000032936; ENSMUSG00000030697.
GeneIDi56420.
KEGGimmu:56420.
UCSCiuc009jss.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF088911 mRNA. Translation: AAC96297.1.
AF378669 Genomic DNA. Translation: AAL35110.1.
BC001993 mRNA. Translation: AAH01993.1.
U79747 mRNA. Translation: AAB38494.1. Frameshift.
CCDSiCCDS21844.1.
RefSeqiNP_062648.1. NM_019674.3.
UniGeneiMm.41998.

3D structure databases

ProteinModelPortaliP97470.
SMRiP97470. Positions 6-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207966. 1 interaction.
IntActiP97470. 1 interaction.
STRINGi10090.ENSMUSP00000032936.

PTM databases

PhosphoSiteiP97470.

Proteomic databases

MaxQBiP97470.
PaxDbiP97470.
PRIDEiP97470.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032936; ENSMUSP00000032936; ENSMUSG00000030697.
GeneIDi56420.
KEGGimmu:56420.
UCSCiuc009jss.1. mouse.

Organism-specific databases

CTDi5531.
MGIiMGI:1891763. Ppp4c.

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00550000074618.
HOGENOMiHOG000172696.
HOVERGENiHBG000216.
InParanoidiP97470.
KOiK15423.
OMAiVFNHRND.
OrthoDBiEOG74N5H2.
PhylomeDBiP97470.
TreeFamiTF105559.

Miscellaneous databases

ChiTaRSiPpp4c. mouse.
NextBioi312568.
PROiP97470.
SOURCEiSearch...

Gene expression databases

BgeeiP97470.
CleanExiMM_PPP4C.
ExpressionAtlasiP97470. baseline and differential.
GenevisibleiP97470. MM.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Protein phosphatase X interacts with c-Rel and stimulates c-Rel/nuclear factor kappaB activity."
    Hu M.C.-T., Tang-Oxley Q., Qiu W.R., Wang Y.-P., Mihindukulasuriya K.A., Afshar R., Tan T.-H.
    J. Biol. Chem. 273:33561-33565(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic structure of the mouse PP4 gene: a developmentally regulated protein phosphatase."
    Hu M.C.-T., Shui J.W., Mihindukulasuriya K.A., Tan T.-H.
    Gene 278:89-99(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  4. Sussman D.J.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 79-293.
    Strain: BALB/c.
  5. "PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4."
    Chen G.I., Tisayakorn S., Jorgensen C., D'Ambrosio L.M., Goudreault M., Gingras A.-C.
    J. Biol. Chem. 283:29273-29284(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPP4C.
  6. "Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation."
    Toyo-oka K., Mori D., Yano Y., Shiota M., Iwao H., Goto H., Inagaki M., Hiraiwa N., Muramatsu M., Wynshaw-Boris A., Yoshiki A., Hirotsune S.
    J. Cell Biol. 180:1133-1147(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPP4C_MOUSE
AccessioniPrimary (citable) accession number: P97470
Secondary accession number(s): P33172
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: July 22, 2015
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.