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P97470

- PP4C_MOUSE

UniProt

P97470 - PP4C_MOUSE

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Protein

Serine/threonine-protein phosphatase 4 catalytic subunit

Gene
Ppp4c, Ppp4, Ppx
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair By similarity. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase. In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin By similarity.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Manganese 1 By similarity
Metal bindingi56 – 561Manganese 1 By similarity
Metal bindingi82 – 821Manganese 1 By similarity
Metal bindingi82 – 821Manganese 2 By similarity
Metal bindingi114 – 1141Manganese 2 By similarity
Active sitei115 – 1151Proton donor By similarity
Metal bindingi164 – 1641Manganese 2 By similarity
Metal bindingi238 – 2381Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. NF-kappaB-inducing kinase activity Source: UniProtKB
  3. protein binding Source: MGI
  4. protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. NIK/NF-kappaB signaling Source: GOC
  3. regulation of double-strand break repair via homologous recombination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit (EC:3.1.3.16)
Short name:
PP4C
Short name:
Pp4
Alternative name(s):
Protein phosphatase X
Short name:
PP-X
Gene namesi
Name:Ppp4c
Synonyms:Ppp4, Ppx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1891763. Ppp4c.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. microtubule organizing center Source: UniProtKB-SubCell
  3. nucleus Source: MGI
  4. plasma membrane Source: Ensembl
  5. protein phosphatase 4 complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 307306Serine/threonine-protein phosphatase 4 catalytic subunitPRO_0000058884Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei307 – 3071Leucine methyl ester By similarity

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

MaxQBiP97470.
PaxDbiP97470.
PRIDEiP97470.

PTM databases

PhosphoSiteiP97470.

Expressioni

Gene expression databases

ArrayExpressiP97470.
BgeeiP97470.
CleanExiMM_PPP4C.
GenevestigatoriP97470.

Interactioni

Subunit structurei

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2. Interacts with REL, NFKB1/p50 and RELA. Interacts with SMN1 AND GEMIN4. Interacts with IRS4 (phosphorylated). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2. Interacts with HDAC3 By similarity.1 Publication

Protein-protein interaction databases

BioGridi207966. 1 interaction.
IntActiP97470. 1 interaction.
STRINGi10090.ENSMUSP00000032936.

Structurei

3D structure databases

ProteinModelPortaliP97470.
SMRiP97470. Positions 6-291.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
HOVERGENiHBG000216.
InParanoidiP97470.
KOiK15423.
OMAiVFNHRND.
OrthoDBiEOG74N5H2.
PhylomeDBiP97470.
TreeFamiTF105559.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97470-1 [UniParc]FASTAAdd to Basket

« Hide

MAEISDLDRQ IEQLRRCELI KESEVKALCA KAREILVEES NVQRVDSPVT    50
VCGDIHGQFY DLKELFRVGG DVPETNYLFM GDFVDRGFYS VETFLLLLAL 100
KVRYPDRITL IRGNHESRQI TQVYGFYDEC LRKYGSVTVW RYCTEIFDYL 150
SLSAIIDGKI FCVHGGLSPS IQTLDQIRTI DRKQEVPHDG PMCDLLWSDP 200
EDTTGWGVSP RGAGYLFGSD VVAQFNAAND IDMICRAHQL VMEGYKWHFN 250
ETVLTVWSAP NYCYRCGNVA AILELDEHLQ KDFIIFEAAP QETRGIPSKK 300
PVADYFL 307
Length:307
Mass (Da):35,080
Last modified:March 1, 2004 - v2
Checksum:iD6FE470A5C6CBCAC
GO

Sequence cautioni

The sequence AAB38494.1 differs from that shown. Reason: Frameshift at positions 101 and 106.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 962LL → FF in AAB38494. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF088911 mRNA. Translation: AAC96297.1.
AF378669 Genomic DNA. Translation: AAL35110.1.
BC001993 mRNA. Translation: AAH01993.1.
U79747 mRNA. Translation: AAB38494.1. Frameshift.
CCDSiCCDS21844.1.
RefSeqiNP_062648.1. NM_019674.3.
UniGeneiMm.41998.

Genome annotation databases

EnsembliENSMUST00000032936; ENSMUSP00000032936; ENSMUSG00000030697.
GeneIDi56420.
KEGGimmu:56420.
UCSCiuc009jss.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF088911 mRNA. Translation: AAC96297.1 .
AF378669 Genomic DNA. Translation: AAL35110.1 .
BC001993 mRNA. Translation: AAH01993.1 .
U79747 mRNA. Translation: AAB38494.1 . Frameshift.
CCDSi CCDS21844.1.
RefSeqi NP_062648.1. NM_019674.3.
UniGenei Mm.41998.

3D structure databases

ProteinModelPortali P97470.
SMRi P97470. Positions 6-291.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207966. 1 interaction.
IntActi P97470. 1 interaction.
STRINGi 10090.ENSMUSP00000032936.

PTM databases

PhosphoSitei P97470.

Proteomic databases

MaxQBi P97470.
PaxDbi P97470.
PRIDEi P97470.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032936 ; ENSMUSP00000032936 ; ENSMUSG00000030697 .
GeneIDi 56420.
KEGGi mmu:56420.
UCSCi uc009jss.1. mouse.

Organism-specific databases

CTDi 5531.
MGIi MGI:1891763. Ppp4c.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000172696.
HOVERGENi HBG000216.
InParanoidi P97470.
KOi K15423.
OMAi VFNHRND.
OrthoDBi EOG74N5H2.
PhylomeDBi P97470.
TreeFami TF105559.

Miscellaneous databases

ChiTaRSi PPP4C. mouse.
NextBioi 21426.
PROi P97470.
SOURCEi Search...

Gene expression databases

ArrayExpressi P97470.
Bgeei P97470.
CleanExi MM_PPP4C.
Genevestigatori P97470.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Protein phosphatase X interacts with c-Rel and stimulates c-Rel/nuclear factor kappaB activity."
    Hu M.C.-T., Tang-Oxley Q., Qiu W.R., Wang Y.-P., Mihindukulasuriya K.A., Afshar R., Tan T.-H.
    J. Biol. Chem. 273:33561-33565(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic structure of the mouse PP4 gene: a developmentally regulated protein phosphatase."
    Hu M.C.-T., Shui J.W., Mihindukulasuriya K.A., Tan T.-H.
    Gene 278:89-99(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  4. Sussman D.J.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 79-293.
    Strain: BALB/c.
  5. "PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4."
    Chen G.I., Tisayakorn S., Jorgensen C., D'Ambrosio L.M., Goudreault M., Gingras A.-C.
    J. Biol. Chem. 283:29273-29284(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPP4C.
  6. "Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation."
    Toyo-oka K., Mori D., Yano Y., Shiota M., Iwao H., Goto H., Inagaki M., Hiraiwa N., Muramatsu M., Wynshaw-Boris A., Yoshiki A., Hirotsune S.
    J. Cell Biol. 180:1133-1147(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPP4C_MOUSE
AccessioniPrimary (citable) accession number: P97470
Secondary accession number(s): P33172
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: July 9, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi