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Protein

Zinc finger protein SNAI2

Gene

Snai2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri129 – 15123C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri160 – 18223C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri186 – 20823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri214 – 23623C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri242 – 26524C2H2-type 5; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • canonical Wnt signaling pathway Source: MGI
  • cartilage morphogenesis Source: MGI
  • cell migration Source: MGI
  • cell migration involved in endocardial cushion formation Source: BHF-UCL
  • cellular response to epidermal growth factor stimulus Source: MGI
  • cellular response to fibroblast growth factor stimulus Source: Ensembl
  • cellular response to ionizing radiation Source: MGI
  • cellular response to platelet-derived growth factor stimulus Source: BHF-UCL
  • desmosome disassembly Source: BHF-UCL
  • epithelial to mesenchymal transition Source: BHF-UCL
  • epithelial to mesenchymal transition involved in endocardial cushion formation Source: MGI
  • epithelium development Source: BHF-UCL
  • negative regulation of anoikis Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of canonical Wnt signaling pathway Source: MGI
  • negative regulation of catenin import into nucleus Source: MGI
  • negative regulation of cell adhesion involved in substrate-bound cell migration Source: MGI
  • negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • negative regulation of chondrocyte differentiation Source: MGI
  • negative regulation of DNA damage response, signal transduction by p53 class mediator Source: MGI
  • negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
  • negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  • negative regulation of keratinocyte proliferation Source: MGI
  • negative regulation of stem cell proliferation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • negative regulation of vitamin D biosynthetic process Source: MGI
  • negative regulation of vitamin D receptor signaling pathway Source: MGI
  • neural crest cell development Source: MGI
  • Notch signaling pathway Source: BHF-UCL
  • osteoblast differentiation Source: Ensembl
  • palate development Source: MGI
  • pigmentation Source: BHF-UCL
  • positive regulation of cell migration Source: MGI
  • positive regulation of endothelial cell chemotaxis Source: BHF-UCL
  • positive regulation of fat cell differentiation Source: MGI
  • positive regulation of histone acetylation Source: MGI
  • regulation of apoptotic process Source: MGI
  • regulation of bicellular tight junction assembly Source: MGI
  • regulation of branching involved in salivary gland morphogenesis Source: MGI
  • regulation of chemokine production Source: MGI
  • regulation of osteoblast differentiation Source: MGI
  • response to radiation Source: MGI
  • sensory perception of sound Source: BHF-UCL
  • transcription from RNA polymerase II promoter Source: GOC
  • white fat cell differentiation Source: MGI
  • wound healing, spreading of cells Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein SNAI2
Alternative name(s):
Neural crest transcription factor Slug
Protein snail homolog 2
Gene namesi
Name:Snai2
Synonyms:Slug, Slugh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1096393. Snai2.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nuclear chromatin Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Zinc finger protein SNAI2PRO_0000047033Add
BLAST

Post-translational modificationi

GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation.By similarity

Proteomic databases

PRIDEiP97469.

PTM databases

PhosphoSiteiP97469.

Expressioni

Gene expression databases

BgeeiP97469.
CleanExiMM_SNAI2.
ExpressionAtlasiP97469. baseline and differential.
GenevisibleiP97469. MM.

Interactioni

Subunit structurei

Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains). Interacts (via zinc fingers) with KPNA2, KPNB1, and TNPO1. May interact (via zinc fingers) with IPO7 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-29680N.
STRINGi10090.ENSMUSP00000023356.

Structurei

3D structure databases

ProteinModelPortaliP97469.
SMRiP97469. Positions 129-265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2020SNAG domainBy similarityAdd
BLAST

Domaini

Repression activity depends on the C-terminal DNA-binding zinc fingers and on the N-terminal repression domain.By similarity

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri129 – 15123C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri160 – 18223C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri186 – 20823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri214 – 23623C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri242 – 26524C2H2-type 5; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
HOGENOMiHOG000261665.
HOVERGENiHBG007477.
InParanoidiP97469.
KOiK05706.
OMAiHINSAKK.
OrthoDBiEOG7P2XSG.
PhylomeDBiP97469.
TreeFamiTF315515.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR029795. SNAIL.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR11389:SF400. PTHR11389:SF400. 1 hit.
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRSFLVKKH FNASKKPNYS ELDTHTVIIS PYLYESYPIP VIPKPEILTS
60 70 80 90 100
GAYSPITVWT SSAAPLHSPL PSGLSPLTGY SSSLGRVSPP PSSDTSSKDH
110 120 130 140 150
SGSESPISDE EERLQPKLSD PHAIEAEKFQ CNLCNKTYST FSGLAKHKQL
160 170 180 190 200
HCDAQSRKSF SCKYCDKEYV SLGALKMHIR THTLPCVCKI CGKAFSRPWL
210 220 230 240 250
LQGHIRTHTG EKPFSCPHCN RAFADRSNLR AHLQTHSDVK KYQCKNCSKT
260
FSRMSLLHKH EESGCCVAH
Length:269
Mass (Da):30,003
Last modified:May 1, 1997 - v1
Checksum:iC61F57779251BEB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97059 mRNA. Translation: AAB58704.1.
U79550 mRNA. Translation: AAB38365.1.
AF079305 Genomic DNA. Translation: AAD23913.1.
BC062164 mRNA. Translation: AAH62164.1.
CCDSiCCDS27974.1.
RefSeqiNP_035545.1. NM_011415.2.
UniGeneiMm.4272.

Genome annotation databases

EnsembliENSMUST00000023356; ENSMUSP00000023356; ENSMUSG00000022676.
GeneIDi20583.
KEGGimmu:20583.
UCSCiuc007yhm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97059 mRNA. Translation: AAB58704.1.
U79550 mRNA. Translation: AAB38365.1.
AF079305 Genomic DNA. Translation: AAD23913.1.
BC062164 mRNA. Translation: AAH62164.1.
CCDSiCCDS27974.1.
RefSeqiNP_035545.1. NM_011415.2.
UniGeneiMm.4272.

3D structure databases

ProteinModelPortaliP97469.
SMRiP97469. Positions 129-265.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29680N.
STRINGi10090.ENSMUSP00000023356.

PTM databases

PhosphoSiteiP97469.

Proteomic databases

PRIDEiP97469.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023356; ENSMUSP00000023356; ENSMUSG00000022676.
GeneIDi20583.
KEGGimmu:20583.
UCSCiuc007yhm.1. mouse.

Organism-specific databases

CTDi6591.
MGIiMGI:1096393. Snai2.

Phylogenomic databases

eggNOGiCOG5048.
HOGENOMiHOG000261665.
HOVERGENiHBG007477.
InParanoidiP97469.
KOiK05706.
OMAiHINSAKK.
OrthoDBiEOG7P2XSG.
PhylomeDBiP97469.
TreeFamiTF315515.

Miscellaneous databases

ChiTaRSiSnai2. mouse.
NextBioi298873.
PROiP97469.
SOURCEiSearch...

Gene expression databases

BgeeiP97469.
CleanExiMM_SNAI2.
ExpressionAtlasiP97469. baseline and differential.
GenevisibleiP97469. MM.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR029795. SNAIL.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR11389:SF400. PTHR11389:SF400. 1 hit.
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The zinc-finger protein Slug causes desmosome dissociation, an initial and necessary step for growth factor-induced epithelial-mesenchymal transition."
    Savagner P., Yamada K.M., Thiery J.P.
    J. Cell Biol. 137:1403-1419(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic organization, expression and chromosomal localization of the mouse Slug (Slugh) gene."
    Jiang R., Norton C.R., Copeland N.G., Gilbert D.J., Jenkins N.A., Gridley T.
    Biochim. Biophys. Acta 1443:251-254(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone.
  4. "Ajuba LIM proteins are snail/slug corepressors required for neural crest development in Xenopus."
    Langer E.M., Feng Y., Zhaoyuan H., Rauscher F.J. III, Kroll K.L., Longmore G.D.
    Dev. Cell 14:424-436(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LIMD1; WTIP AND AJUBA.

Entry informationi

Entry nameiSNAI2_MOUSE
AccessioniPrimary (citable) accession number: P97469
Secondary accession number(s): O09096, Q9R211
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: June 24, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.