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P97469 (SNAI2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein SNAI2
Alternative name(s):
Neural crest transcription factor Slug
Protein snail homolog 2
Gene names
Name:Snai2
Synonyms:Slug, Slugh
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis By similarity.

Subunit structure

Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains). Interacts (via zinc fingers) with KPNA2, KPNB1, and TNPO1. May interact (via zinc fingers) with IPO7 By similarity. Ref.4

Subcellular location

Nucleus By similarity. Cytoplasm By similarity. Note: Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions By similarity.

Domain

Repression activity depends on the C-terminal DNA-binding zinc fingers and on the N-terminal repression domain By similarity.

Post-translational modification

GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation By similarity.

Sequence similarities

Belongs to the snail C2H2-type zinc-finger protein family.

Contains 5 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionDevelopmental protein
Repressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNotch signaling pathway

Inferred from sequence or structural similarity. Source: BHF-UCL

canonical Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

cartilage morphogenesis

Inferred from genetic interaction PubMed 17376812. Source: MGI

cell migration

Inferred from genetic interaction PubMed 17376812. Source: MGI

cell migration involved in endocardial cushion formation

Inferred from mutant phenotype PubMed 18663143. Source: BHF-UCL

cellular response to epidermal growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to fibroblast growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to ionizing radiation

Inferred from genetic interaction PubMed 16286009. Source: MGI

cellular response to platelet-derived growth factor stimulus

Traceable author statement PubMed 18663143. Source: BHF-UCL

desmosome disassembly

Inferred from mutant phenotype Ref.1. Source: BHF-UCL

embryo development

Inferred from electronic annotation. Source: Ensembl

epithelial to mesenchymal transition

Inferred from mutant phenotype Ref.1. Source: BHF-UCL

epithelial to mesenchymal transition involved in endocardial cushion formation

Inferred from mutant phenotype PubMed 18663143. Source: MGI

epithelium development

Inferred from mutant phenotype PubMed 18716062. Source: BHF-UCL

negative regulation of DNA damage response, signal transduction by p53 class mediator

Inferred from electronic annotation. Source: Ensembl

negative regulation of anoikis

Inferred from electronic annotation. Source: Ensembl

negative regulation of apoptotic process

Inferred from genetic interaction PubMed 16286009. Source: MGI

negative regulation of canonical Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

negative regulation of catenin import into nucleus

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell adhesion involved in substrate-bound cell migration

Inferred from mutant phenotype PubMed 18663143. Source: MGI

negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

negative regulation of chondrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

Inferred from direct assay PubMed 10518215. Source: BHF-UCL

negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage

Inferred from electronic annotation. Source: Ensembl

negative regulation of keratinocyte proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of stem cell proliferation

Inferred from mutant phenotype PubMed 20032500. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18223155. Source: MGI

negative regulation of vitamin D biosynthetic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of vitamin D receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

neural crest cell development

Inferred from electronic annotation. Source: Ensembl

osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

palate development

Inferred from mutant phenotype PubMed 17376812. Source: MGI

pigmentation

Inferred from mutant phenotype PubMed 12444107. Source: BHF-UCL

positive regulation of endothelial cell chemotaxis

Inferred by curator PubMed 18663143. Source: BHF-UCL

positive regulation of fat cell differentiation

Inferred from mutant phenotype PubMed 17905753. Source: MGI

positive regulation of histone acetylation

Inferred from mutant phenotype PubMed 17905753. Source: MGI

regulation of apoptotic process

Inferred by curator PubMed 12833143. Source: MGI

regulation of branching involved in salivary gland morphogenesis

Inferred from mutant phenotype PubMed 20671187. Source: MGI

regulation of chemokine production

Inferred from electronic annotation. Source: Ensembl

regulation of osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

regulation of tight junction assembly

Inferred from electronic annotation. Source: Ensembl

response to radiation

Inferred from direct assay PubMed 12833143. Source: MGI

sensory perception of sound

Inferred from mutant phenotype PubMed 12444107. Source: BHF-UCL

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18223155. Source: GOC

white fat cell differentiation

Inferred from mutant phenotype PubMed 17905753. Source: MGI

wound healing, spreading of cells

Traceable author statement PubMed 18663143. Source: BHF-UCL

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 20046880. Source: MGI

nuclear chromatin

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 12833143PubMed 20046880. Source: MGI

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription

Inferred from direct assay PubMed 18663143. Source: MGI

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 18223155. Source: MGI

chromatin binding

Inferred from direct assay PubMed 17905753. Source: MGI

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction Ref.4. Source: UniProtKB

sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269Zinc finger protein SNAI2
PRO_0000047033

Regions

Zinc finger129 – 15123C2H2-type 1
Zinc finger160 – 18223C2H2-type 2
Zinc finger186 – 20823C2H2-type 3
Zinc finger214 – 23623C2H2-type 4
Zinc finger242 – 26524C2H2-type 5; atypical
Region1 – 2020SNAG domain By similarity

Sequences

Sequence LengthMass (Da)Tools
P97469 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: C61F57779251BEB0

FASTA26930,003
        10         20         30         40         50         60 
MPRSFLVKKH FNASKKPNYS ELDTHTVIIS PYLYESYPIP VIPKPEILTS GAYSPITVWT 

        70         80         90        100        110        120 
SSAAPLHSPL PSGLSPLTGY SSSLGRVSPP PSSDTSSKDH SGSESPISDE EERLQPKLSD 

       130        140        150        160        170        180 
PHAIEAEKFQ CNLCNKTYST FSGLAKHKQL HCDAQSRKSF SCKYCDKEYV SLGALKMHIR 

       190        200        210        220        230        240 
THTLPCVCKI CGKAFSRPWL LQGHIRTHTG EKPFSCPHCN RAFADRSNLR AHLQTHSDVK 

       250        260 
KYQCKNCSKT FSRMSLLHKH EESGCCVAH 

« Hide

References

« Hide 'large scale' references
[1]"The zinc-finger protein Slug causes desmosome dissociation, an initial and necessary step for growth factor-induced epithelial-mesenchymal transition."
Savagner P., Yamada K.M., Thiery J.P.
J. Cell Biol. 137:1403-1419(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Genomic organization, expression and chromosomal localization of the mouse Slug (Slugh) gene."
Jiang R., Norton C.R., Copeland N.G., Gilbert D.J., Jenkins N.A., Gridley T.
Biochim. Biophys. Acta 1443:251-254(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Bone.
[4]"Ajuba LIM proteins are snail/slug corepressors required for neural crest development in Xenopus."
Langer E.M., Feng Y., Zhaoyuan H., Rauscher F.J. III, Kroll K.L., Longmore G.D.
Dev. Cell 14:424-436(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LIMD1; WTIP AND AJUBA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U97059 mRNA. Translation: AAB58704.1.
U79550 mRNA. Translation: AAB38365.1.
AF079305 Genomic DNA. Translation: AAD23913.1.
BC062164 mRNA. Translation: AAH62164.1.
CCDSCCDS27974.1.
RefSeqNP_035545.1. NM_011415.2.
UniGeneMm.4272.

3D structure databases

ProteinModelPortalP97469.
SMRP97469. Positions 129-265.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-29680N.
STRING10090.ENSMUSP00000023356.

PTM databases

PhosphoSiteP97469.

Proteomic databases

PRIDEP97469.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023356; ENSMUSP00000023356; ENSMUSG00000022676.
GeneID20583.
KEGGmmu:20583.
UCSCuc007yhm.1. mouse.

Organism-specific databases

CTD6591.
MGIMGI:1096393. Snai2.

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000261665.
HOVERGENHBG007477.
InParanoidP97469.
KOK05706.
OMAEDEQMLP.
OrthoDBEOG7P2XSG.
PhylomeDBP97469.
TreeFamTF315515.

Gene expression databases

ArrayExpressP97469.
BgeeP97469.
CleanExMM_SNAI2.
GenevestigatorP97469.

Family and domain databases

Gene3D3.30.160.60. 4 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSNAI2. mouse.
NextBio298873.
PROP97469.
SOURCESearch...

Entry information

Entry nameSNAI2_MOUSE
AccessionPrimary (citable) accession number: P97469
Secondary accession number(s): O09096, Q9R211
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot