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P97469

- SNAI2_MOUSE

UniProt

P97469 - SNAI2_MOUSE

Protein

Zinc finger protein SNAI2

Gene

Snai2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri129 – 15123C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri160 – 18223C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri186 – 20823C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri214 – 23623C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri242 – 26524C2H2-type 5; atypicalPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: MGI
    5. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
    6. sequence-specific DNA binding Source: Ensembl

    GO - Biological processi

    1. canonical Wnt signaling pathway Source: Ensembl
    2. cartilage morphogenesis Source: MGI
    3. cell migration Source: MGI
    4. cell migration involved in endocardial cushion formation Source: BHF-UCL
    5. cellular response to epidermal growth factor stimulus Source: Ensembl
    6. cellular response to fibroblast growth factor stimulus Source: Ensembl
    7. cellular response to ionizing radiation Source: MGI
    8. cellular response to platelet-derived growth factor stimulus Source: BHF-UCL
    9. desmosome disassembly Source: BHF-UCL
    10. embryo development Source: Ensembl
    11. epithelial to mesenchymal transition Source: BHF-UCL
    12. epithelial to mesenchymal transition involved in endocardial cushion formation Source: MGI
    13. epithelium development Source: BHF-UCL
    14. negative regulation of anoikis Source: Ensembl
    15. negative regulation of apoptotic process Source: MGI
    16. negative regulation of canonical Wnt signaling pathway Source: Ensembl
    17. negative regulation of catenin import into nucleus Source: Ensembl
    18. negative regulation of cell adhesion involved in substrate-bound cell migration Source: MGI
    19. negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    20. negative regulation of chondrocyte differentiation Source: Ensembl
    21. negative regulation of DNA damage response, signal transduction by p53 class mediator Source: Ensembl
    22. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
    23. negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: Ensembl
    24. negative regulation of keratinocyte proliferation Source: Ensembl
    25. negative regulation of stem cell proliferation Source: MGI
    26. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    27. negative regulation of vitamin D biosynthetic process Source: Ensembl
    28. negative regulation of vitamin D receptor signaling pathway Source: Ensembl
    29. neural crest cell development Source: Ensembl
    30. Notch signaling pathway Source: BHF-UCL
    31. osteoblast differentiation Source: Ensembl
    32. palate development Source: MGI
    33. pigmentation Source: BHF-UCL
    34. positive regulation of endothelial cell chemotaxis Source: BHF-UCL
    35. positive regulation of fat cell differentiation Source: MGI
    36. positive regulation of histone acetylation Source: MGI
    37. regulation of apoptotic process Source: MGI
    38. regulation of branching involved in salivary gland morphogenesis Source: MGI
    39. regulation of chemokine production Source: Ensembl
    40. regulation of osteoblast differentiation Source: Ensembl
    41. regulation of tight junction assembly Source: Ensembl
    42. response to radiation Source: MGI
    43. sensory perception of sound Source: BHF-UCL
    44. transcription from RNA polymerase II promoter Source: GOC
    45. white fat cell differentiation Source: MGI
    46. wound healing, spreading of cells Source: BHF-UCL

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger protein SNAI2
    Alternative name(s):
    Neural crest transcription factor Slug
    Protein snail homolog 2
    Gene namesi
    Name:Snai2
    Synonyms:Slug, Slugh
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1096393. Snai2.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity
    Note: Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nuclear chromatin Source: Ensembl
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 269269Zinc finger protein SNAI2PRO_0000047033Add
    BLAST

    Post-translational modificationi

    GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation.By similarity

    Proteomic databases

    PRIDEiP97469.

    PTM databases

    PhosphoSiteiP97469.

    Expressioni

    Gene expression databases

    ArrayExpressiP97469.
    BgeeiP97469.
    CleanExiMM_SNAI2.
    GenevestigatoriP97469.

    Interactioni

    Subunit structurei

    Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains). Interacts (via zinc fingers) with KPNA2, KPNB1, and TNPO1. May interact (via zinc fingers) with IPO7 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-29680N.
    STRINGi10090.ENSMUSP00000023356.

    Structurei

    3D structure databases

    ProteinModelPortaliP97469.
    SMRiP97469. Positions 129-265.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2020SNAG domainBy similarityAdd
    BLAST

    Domaini

    Repression activity depends on the C-terminal DNA-binding zinc fingers and on the N-terminal repression domain.By similarity

    Sequence similaritiesi

    Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri129 – 15123C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri160 – 18223C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri186 – 20823C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri214 – 23623C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri242 – 26524C2H2-type 5; atypicalPROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    HOGENOMiHOG000261665.
    HOVERGENiHBG007477.
    InParanoidiP97469.
    KOiK05706.
    OMAiEDEQMLP.
    OrthoDBiEOG7P2XSG.
    PhylomeDBiP97469.
    TreeFamiTF315515.

    Family and domain databases

    Gene3Di3.30.160.60. 4 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00096. zf-C2H2. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 5 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 5 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P97469-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPRSFLVKKH FNASKKPNYS ELDTHTVIIS PYLYESYPIP VIPKPEILTS    50
    GAYSPITVWT SSAAPLHSPL PSGLSPLTGY SSSLGRVSPP PSSDTSSKDH 100
    SGSESPISDE EERLQPKLSD PHAIEAEKFQ CNLCNKTYST FSGLAKHKQL 150
    HCDAQSRKSF SCKYCDKEYV SLGALKMHIR THTLPCVCKI CGKAFSRPWL 200
    LQGHIRTHTG EKPFSCPHCN RAFADRSNLR AHLQTHSDVK KYQCKNCSKT 250
    FSRMSLLHKH EESGCCVAH 269
    Length:269
    Mass (Da):30,003
    Last modified:May 1, 1997 - v1
    Checksum:iC61F57779251BEB0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U97059 mRNA. Translation: AAB58704.1.
    U79550 mRNA. Translation: AAB38365.1.
    AF079305 Genomic DNA. Translation: AAD23913.1.
    BC062164 mRNA. Translation: AAH62164.1.
    CCDSiCCDS27974.1.
    RefSeqiNP_035545.1. NM_011415.2.
    UniGeneiMm.4272.

    Genome annotation databases

    EnsembliENSMUST00000023356; ENSMUSP00000023356; ENSMUSG00000022676.
    GeneIDi20583.
    KEGGimmu:20583.
    UCSCiuc007yhm.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U97059 mRNA. Translation: AAB58704.1 .
    U79550 mRNA. Translation: AAB38365.1 .
    AF079305 Genomic DNA. Translation: AAD23913.1 .
    BC062164 mRNA. Translation: AAH62164.1 .
    CCDSi CCDS27974.1.
    RefSeqi NP_035545.1. NM_011415.2.
    UniGenei Mm.4272.

    3D structure databases

    ProteinModelPortali P97469.
    SMRi P97469. Positions 129-265.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29680N.
    STRINGi 10090.ENSMUSP00000023356.

    PTM databases

    PhosphoSitei P97469.

    Proteomic databases

    PRIDEi P97469.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023356 ; ENSMUSP00000023356 ; ENSMUSG00000022676 .
    GeneIDi 20583.
    KEGGi mmu:20583.
    UCSCi uc007yhm.1. mouse.

    Organism-specific databases

    CTDi 6591.
    MGIi MGI:1096393. Snai2.

    Phylogenomic databases

    eggNOGi COG5048.
    HOGENOMi HOG000261665.
    HOVERGENi HBG007477.
    InParanoidi P97469.
    KOi K05706.
    OMAi EDEQMLP.
    OrthoDBi EOG7P2XSG.
    PhylomeDBi P97469.
    TreeFami TF315515.

    Miscellaneous databases

    ChiTaRSi SNAI2. mouse.
    NextBioi 298873.
    PROi P97469.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97469.
    Bgeei P97469.
    CleanExi MM_SNAI2.
    Genevestigatori P97469.

    Family and domain databases

    Gene3Di 3.30.160.60. 4 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00096. zf-C2H2. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 5 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 5 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The zinc-finger protein Slug causes desmosome dissociation, an initial and necessary step for growth factor-induced epithelial-mesenchymal transition."
      Savagner P., Yamada K.M., Thiery J.P.
      J. Cell Biol. 137:1403-1419(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Genomic organization, expression and chromosomal localization of the mouse Slug (Slugh) gene."
      Jiang R., Norton C.R., Copeland N.G., Gilbert D.J., Jenkins N.A., Gridley T.
      Biochim. Biophys. Acta 1443:251-254(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Bone.
    4. "Ajuba LIM proteins are snail/slug corepressors required for neural crest development in Xenopus."
      Langer E.M., Feng Y., Zhaoyuan H., Rauscher F.J. III, Kroll K.L., Longmore G.D.
      Dev. Cell 14:424-436(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LIMD1; WTIP AND AJUBA.

    Entry informationi

    Entry nameiSNAI2_MOUSE
    AccessioniPrimary (citable) accession number: P97469
    Secondary accession number(s): O09096, Q9R211
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3