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P97469

- SNAI2_MOUSE

UniProt

P97469 - SNAI2_MOUSE

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Protein

Zinc finger protein SNAI2

Gene

Snai2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri129 – 15123C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri160 – 18223C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri186 – 20823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri214 – 23623C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri242 – 26524C2H2-type 5; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: MGI
  4. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
  5. sequence-specific DNA binding Source: Ensembl

GO - Biological processi

  1. canonical Wnt signaling pathway Source: Ensembl
  2. cartilage morphogenesis Source: MGI
  3. cell migration Source: MGI
  4. cell migration involved in endocardial cushion formation Source: BHF-UCL
  5. cellular response to epidermal growth factor stimulus Source: Ensembl
  6. cellular response to fibroblast growth factor stimulus Source: Ensembl
  7. cellular response to ionizing radiation Source: MGI
  8. cellular response to platelet-derived growth factor stimulus Source: BHF-UCL
  9. desmosome disassembly Source: BHF-UCL
  10. embryo development Source: Ensembl
  11. epithelial to mesenchymal transition Source: BHF-UCL
  12. epithelial to mesenchymal transition involved in endocardial cushion formation Source: MGI
  13. epithelium development Source: BHF-UCL
  14. negative regulation of anoikis Source: Ensembl
  15. negative regulation of apoptotic process Source: MGI
  16. negative regulation of canonical Wnt signaling pathway Source: Ensembl
  17. negative regulation of catenin import into nucleus Source: Ensembl
  18. negative regulation of cell adhesion involved in substrate-bound cell migration Source: MGI
  19. negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  20. negative regulation of chondrocyte differentiation Source: Ensembl
  21. negative regulation of DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  22. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
  23. negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: Ensembl
  24. negative regulation of keratinocyte proliferation Source: Ensembl
  25. negative regulation of stem cell proliferation Source: MGI
  26. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  27. negative regulation of vitamin D biosynthetic process Source: Ensembl
  28. negative regulation of vitamin D receptor signaling pathway Source: Ensembl
  29. neural crest cell development Source: Ensembl
  30. Notch signaling pathway Source: BHF-UCL
  31. osteoblast differentiation Source: Ensembl
  32. palate development Source: MGI
  33. pigmentation Source: BHF-UCL
  34. positive regulation of endothelial cell chemotaxis Source: BHF-UCL
  35. positive regulation of fat cell differentiation Source: MGI
  36. positive regulation of histone acetylation Source: MGI
  37. regulation of apoptotic process Source: MGI
  38. regulation of branching involved in salivary gland morphogenesis Source: MGI
  39. regulation of chemokine production Source: Ensembl
  40. regulation of osteoblast differentiation Source: Ensembl
  41. regulation of tight junction assembly Source: Ensembl
  42. response to radiation Source: MGI
  43. sensory perception of sound Source: BHF-UCL
  44. transcription from RNA polymerase II promoter Source: GOC
  45. white fat cell differentiation Source: MGI
  46. wound healing, spreading of cells Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein SNAI2
Alternative name(s):
Neural crest transcription factor Slug
Protein snail homolog 2
Gene namesi
Name:Snai2
Synonyms:Slug, Slugh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1096393. Snai2.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity
Note: Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nuclear chromatin Source: Ensembl
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Zinc finger protein SNAI2PRO_0000047033Add
BLAST

Post-translational modificationi

GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation.By similarity

Proteomic databases

PRIDEiP97469.

PTM databases

PhosphoSiteiP97469.

Expressioni

Gene expression databases

BgeeiP97469.
CleanExiMM_SNAI2.
ExpressionAtlasiP97469. baseline and differential.
GenevestigatoriP97469.

Interactioni

Subunit structurei

Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains). Interacts (via zinc fingers) with KPNA2, KPNB1, and TNPO1. May interact (via zinc fingers) with IPO7 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-29680N.
STRINGi10090.ENSMUSP00000023356.

Structurei

3D structure databases

ProteinModelPortaliP97469.
SMRiP97469. Positions 129-265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2020SNAG domainBy similarityAdd
BLAST

Domaini

Repression activity depends on the C-terminal DNA-binding zinc fingers and on the N-terminal repression domain.By similarity

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri129 – 15123C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri160 – 18223C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri186 – 20823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri214 – 23623C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri242 – 26524C2H2-type 5; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
HOGENOMiHOG000261665.
HOVERGENiHBG007477.
InParanoidiP97469.
KOiK05706.
OMAiEDEQMLP.
OrthoDBiEOG7P2XSG.
PhylomeDBiP97469.
TreeFamiTF315515.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97469 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPRSFLVKKH FNASKKPNYS ELDTHTVIIS PYLYESYPIP VIPKPEILTS
60 70 80 90 100
GAYSPITVWT SSAAPLHSPL PSGLSPLTGY SSSLGRVSPP PSSDTSSKDH
110 120 130 140 150
SGSESPISDE EERLQPKLSD PHAIEAEKFQ CNLCNKTYST FSGLAKHKQL
160 170 180 190 200
HCDAQSRKSF SCKYCDKEYV SLGALKMHIR THTLPCVCKI CGKAFSRPWL
210 220 230 240 250
LQGHIRTHTG EKPFSCPHCN RAFADRSNLR AHLQTHSDVK KYQCKNCSKT
260
FSRMSLLHKH EESGCCVAH
Length:269
Mass (Da):30,003
Last modified:May 1, 1997 - v1
Checksum:iC61F57779251BEB0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U97059 mRNA. Translation: AAB58704.1.
U79550 mRNA. Translation: AAB38365.1.
AF079305 Genomic DNA. Translation: AAD23913.1.
BC062164 mRNA. Translation: AAH62164.1.
CCDSiCCDS27974.1.
RefSeqiNP_035545.1. NM_011415.2.
UniGeneiMm.4272.

Genome annotation databases

EnsembliENSMUST00000023356; ENSMUSP00000023356; ENSMUSG00000022676.
GeneIDi20583.
KEGGimmu:20583.
UCSCiuc007yhm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U97059 mRNA. Translation: AAB58704.1 .
U79550 mRNA. Translation: AAB38365.1 .
AF079305 Genomic DNA. Translation: AAD23913.1 .
BC062164 mRNA. Translation: AAH62164.1 .
CCDSi CCDS27974.1.
RefSeqi NP_035545.1. NM_011415.2.
UniGenei Mm.4272.

3D structure databases

ProteinModelPortali P97469.
SMRi P97469. Positions 129-265.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29680N.
STRINGi 10090.ENSMUSP00000023356.

PTM databases

PhosphoSitei P97469.

Proteomic databases

PRIDEi P97469.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023356 ; ENSMUSP00000023356 ; ENSMUSG00000022676 .
GeneIDi 20583.
KEGGi mmu:20583.
UCSCi uc007yhm.1. mouse.

Organism-specific databases

CTDi 6591.
MGIi MGI:1096393. Snai2.

Phylogenomic databases

eggNOGi COG5048.
HOGENOMi HOG000261665.
HOVERGENi HBG007477.
InParanoidi P97469.
KOi K05706.
OMAi EDEQMLP.
OrthoDBi EOG7P2XSG.
PhylomeDBi P97469.
TreeFami TF315515.

Miscellaneous databases

ChiTaRSi SNAI2. mouse.
NextBioi 298873.
PROi P97469.
SOURCEi Search...

Gene expression databases

Bgeei P97469.
CleanExi MM_SNAI2.
ExpressionAtlasi P97469. baseline and differential.
Genevestigatori P97469.

Family and domain databases

Gene3Di 3.30.160.60. 4 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF00096. zf-C2H2. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 5 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The zinc-finger protein Slug causes desmosome dissociation, an initial and necessary step for growth factor-induced epithelial-mesenchymal transition."
    Savagner P., Yamada K.M., Thiery J.P.
    J. Cell Biol. 137:1403-1419(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic organization, expression and chromosomal localization of the mouse Slug (Slugh) gene."
    Jiang R., Norton C.R., Copeland N.G., Gilbert D.J., Jenkins N.A., Gridley T.
    Biochim. Biophys. Acta 1443:251-254(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone.
  4. "Ajuba LIM proteins are snail/slug corepressors required for neural crest development in Xenopus."
    Langer E.M., Feng Y., Zhaoyuan H., Rauscher F.J. III, Kroll K.L., Longmore G.D.
    Dev. Cell 14:424-436(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LIMD1; WTIP AND AJUBA.

Entry informationi

Entry nameiSNAI2_MOUSE
AccessioniPrimary (citable) accession number: P97469
Secondary accession number(s): O09096, Q9R211
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3