P97469 (SNAI2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein SNAI2

Alternative name(s):
Neural crest transcription factor Slug
Protein snail homolog 2

Gene names

Name:	Snai2
Synonyms:	Slug, Slugh

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis By similarity.
Subunit structure	Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains). Interacts (via zinc fingers) with KPNA2, KPNB1, and TNPO1. May interact (via zinc fingers) with IPO7 By similarity. Ref.4
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Note: Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions By similarity.
Domain	Repression activity depends on the C-terminal DNA-binding zinc fingers and on the N-terminal repression domain By similarity.
Post-translational modification	GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation By similarity.
Sequence similarities	Belongs to the snail C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Notch signaling pathway Inferred from sequence or structural similarity. Source: BHF-UCL canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara cartilage morphogenesis Inferred from genetic interaction PubMed 17376812. Source: MGI cell migration involved in endocardial cushion formation Inferred from mutant phenotype PubMed 18663143. Source: BHF-UCL cellular response to epidermal growth factor stimulus Inferred from electronic annotation. Source: Compara cellular response to fibroblast growth factor stimulus Inferred from electronic annotation. Source: Compara cellular response to ionizing radiation Inferred from genetic interaction PubMed 16286009. Source: MGI cellular response to platelet-derived growth factor stimulus Traceable author statement PubMed 18663143. Source: BHF-UCL desmosome disassembly Inferred from mutant phenotype Ref.1. Source: BHF-UCL embryo development Inferred from electronic annotation. Source: Compara epithelial to mesenchymal transition involved in endocardial cushion formation Inferred from mutant phenotype PubMed 18663143. Source: MGI negative regulation of DNA damage response, signal transduction by p53 class mediator Inferred from electronic annotation. Source: Compara negative regulation of anoikis Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from direct assay PubMed 10518215. Source: BHF-UCL negative regulation of canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of catenin import into nucleus Inferred from electronic annotation. Source: Compara negative regulation of cell adhesion involved in substrate-bound cell migration Inferred from mutant phenotype PubMed 18663143. Source: MGI negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara negative regulation of chondrocyte differentiation Inferred from electronic annotation. Source: Compara negative regulation of keratinocyte proliferation Inferred from electronic annotation. Source: Compara negative regulation of stem cell proliferation Inferred from mutant phenotype PubMed 20032500. Source: MGI negative regulation of vitamin D biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of vitamin D receptor signaling pathway Inferred from electronic annotation. Source: Compara neural crest cell development Inferred from electronic annotation. Source: Compara osteoblast differentiation Inferred from electronic annotation. Source: Compara palate development Inferred from mutant phenotype PubMed 17376812. Source: MGI pigmentation Inferred from mutant phenotype PubMed 12444107. Source: BHF-UCL positive regulation of endothelial cell chemotaxis Inferred by curator PubMed 18663143. Source: BHF-UCL positive regulation of fat cell differentiation Inferred from mutant phenotype PubMed 17905753. Source: MGI positive regulation of histone acetylation Inferred from mutant phenotype PubMed 17905753. Source: MGI regulation of chemokine production Inferred from electronic annotation. Source: Compara regulation of osteoblast differentiation Inferred from electronic annotation. Source: Compara regulation of tight junction assembly Inferred from electronic annotation. Source: Compara sensory perception of sound Inferred from mutant phenotype PubMed 12444107. Source: BHF-UCL white fat cell differentiation Inferred from mutant phenotype PubMed 17905753. Source: MGI wound healing, spreading of cells Traceable author statement PubMed 18663143. Source: BHF-UCL
Cellular_component	cytoplasm Inferred from direct assay PubMed 20046880. Source: MGI nuclear chromatin Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay PubMed 12833143PubMed 20046880. Source: MGI
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Inferred from direct assay PubMed 18663143. Source: MGI RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 18223155. Source: MGI chromatin binding Inferred from direct assay PubMed 17905753. Source: MGI sequence-specific DNA binding Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Zinc finger protein SNAI2		PRO_0000047033
Regions
	Zinc finger	129 – 151	23	C2H2-type 1
	Zinc finger	160 – 182	23	C2H2-type 2
	Zinc finger	186 – 208	23	C2H2-type 3
	Zinc finger	214 – 236	23	C2H2-type 4
	Zinc finger	242 – 265	24	C2H2-type 5; atypical
	Region	1 – 20	20	SNAG domain By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97469 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: C61F57779251BEB0	FASTA	269	30,003
	10 20 30 40 50 60 MPRSFLVKKH FNASKKPNYS ELDTHTVIIS PYLYESYPIP VIPKPEILTS GAYSPITVWT 70 80 90 100 110 120 SSAAPLHSPL PSGLSPLTGY SSSLGRVSPP PSSDTSSKDH SGSESPISDE EERLQPKLSD 130 140 150 160 170 180 PHAIEAEKFQ CNLCNKTYST FSGLAKHKQL HCDAQSRKSF SCKYCDKEYV SLGALKMHIR 190 200 210 220 230 240 THTLPCVCKI CGKAFSRPWL LQGHIRTHTG EKPFSCPHCN RAFADRSNLR AHLQTHSDVK 250 260 KYQCKNCSKT FSRMSLLHKH EESGCCVAH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The zinc-finger protein Slug causes desmosome dissociation, an initial and necessary step for growth factor-induced epithelial-mesenchymal transition." Savagner P., Yamada K.M., Thiery J.P. J. Cell Biol. 137:1403-1419(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genomic organization, expression and chromosomal localization of the mouse Slug (Slugh) gene." Jiang R., Norton C.R., Copeland N.G., Gilbert D.J., Jenkins N.A., Gridley T. Biochim. Biophys. Acta 1443:251-254(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone.
[4]	"Ajuba LIM proteins are snail/slug corepressors required for neural crest development in Xenopus." Langer E.M., Feng Y., Zhaoyuan H., Rauscher F.J. III, Kroll K.L., Longmore G.D. Dev. Cell 14:424-436(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LIMD1; WTIP AND AJUBA.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U97059 mRNA. Translation: AAB58704.1. U79550 mRNA. Translation: AAB38365.1. AF079305 Genomic DNA. Translation: AAD23913.1. BC062164 mRNA. Translation: AAH62164.1.
IPI	IPI00125552.
RefSeq	NP_035545.1. NM_011415.2.
UniGene	Mm.4272.
3D structure databases
ProteinModelPortal	P97469.
SMR	P97469. Positions 129-263.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29680N.
STRING	10090.ENSMUSP00000023356.
PTM databases
PhosphoSite	P97469.
Proteomic databases
PRIDE	P97469.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023356; ENSMUSP00000023356; ENSMUSG00000022676.
GeneID	20583.
KEGG	mmu:20583.
Organism-specific databases
CTD	6591.
MGI	MGI:1096393. Snai2.
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000261665.
HOVERGEN	HBG007477.
InParanoid	P97469.
KO	K05706.
OMA	FHSPLPN.
OrthoDB	EOG4H9XM9.
Gene expression databases
ArrayExpress	P97469.
Bgee	P97469.
CleanEx	MM_SNAI2.
Genevestigator	P97469.
GermOnline	ENSMUSG00000022676. Mus musculus.
Family and domain databases
Gene3D	3.30.160.60. 4 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 5 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 4 hits. PS50157. ZINC_FINGER_C2H2_2. 5 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SNAI2. mouse.
NextBio	298873.
SOURCE	Search...

Entry information

Entry name

SNAI2_MOUSE

Accession

Primary (citable) accession number: P97469
Secondary accession number(s): O09096, Q9R211

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program