Chemokine-like receptor 1 - Mus musculus (Mouse)

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P97468 (CML1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chemokine-like receptor 1

Alternative name(s):
G-protein coupled receptor DEZ

Gene names

Name:	Cmklr1
Synonyms:	Dez, Gpcr27

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B By similarity. Ref.3 Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.4.
Tissue specificity	Highly expressed in immature plasmacytoid dendritic cells (DCs) and at lower levels in myeloid DCs, macrophages, and NK cells. Expressed on macrophages isolated from different tissues, including peritoneal cavities, pleural cavities and spleen. Ref.4 Ref.5
Developmental stage	Expressed during bone development and in adult parathyroid glands. Expressed at day E11 in the caudal part of the tongue and the umbilical cord and the expression in the tongue was maintained throughout adulthood. Expression increases in bone and cartilaginous forming zones of embryo up to stage E14.5 and at E16.5 expression is seen in the lung. Ref.1
Induction	Down-regulated by bacterial lipopolysaccharide (LPS), IFN-alpha and TNF on macrophages. Ref.4
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chemotaxis Inferred from electronic annotation. Source: Compara negative regulation of NF-kappaB transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of interleukin-12 production Inferred from mutant phenotype Ref.3. Source: UniProtKB positive regulation of macrophage chemotaxis Inferred from electronic annotation. Source: Compara regulation of calcium-mediated signaling Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 371

371

Chemokine-like receptor 1

PRO_0000069308

Regions

Topological domain

1 – 39

Extracellular Potential

Transmembrane

40 – 62

Helical; Name=1; Potential

Topological domain

63 – 73

Cytoplasmic Potential

Transmembrane

74 – 95

Helical; Name=2; Potential

Topological domain

96 – 112

Extracellular Potential

Transmembrane

113 – 133

Helical; Name=3; Potential

Topological domain

134 – 152

Cytoplasmic Potential

Transmembrane

153 – 174

Helical; Name=4; Potential

Topological domain

175 – 222

Extracellular Potential

Transmembrane

223 – 243

Helical; Name=5; Potential

Topological domain

244 – 259

Cytoplasmic Potential

Transmembrane

260 – 280

Helical; Name=6; Potential

Topological domain

281 – 298

Extracellular Potential

Transmembrane

299 – 318

Helical; Name=7; Potential

Topological domain

319 – 371

Cytoplasmic Potential

Amino acid modifications

Modified residue

337

Phosphoserine Ref.2

Modified residue

340

Phosphothreonine Ref.2

Modified residue

347

Phosphoserine Ref.2

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

190

N-linked (GlcNAc...) Potential

Disulfide bond

110 ↔ 187

Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P97468 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: CDBE19305244C0B3
Show »

FASTA

371

41,815

        10         20         30         40         50         60 
MEYDAYNDSG IYDDEYSDGF GYFVDLEEAS PWEAKVAPVF LVVIYSLVCF LGLLGNGLVI 

        70         80         90        100        110        120 
VIATFKMKKT VNTVWFVNLA VADFLFNIFL PMHITYAAMD YHWVFGKAMC KISNFLLSHN 

       130        140        150        160        170        180 
MYTSVFLLTV ISFDRCISVL LPVWSQNHRS IRLAYMTCSA VWVLAFFLSS PSLVFRDTAN 

       190        200        210        220        230        240 
IHGKITCFNN FSLAAPESSP HPAHSQVVST GYSRHVAVTV TRFLCGFLIP VFIITACYLT 

       250        260        270        280        290        300 
IVFKLQRNRL AKNKKPFKII ITIIITFFLC WCPYHTLYLL ELHHTAVPSS VFSLGLPLAT 

       310        320        330        340        350        360 
AVAIANSCMN PILYVFMGHD FRKFKVALFS RLANALSEDT GPSSYPSHRS FTKMSSLNEK 

       370 
ASVNEKETST L

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel G protein-coupled receptor with homology to neuropeptide and chemoattractant receptors expressed during bone development." Methner A., Hermey G., Schinke B., Hermans-Borgmeyer I. Biochem. Biophys. Res. Commun. 233:336-342(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Tissue: Brain.
[2]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337; THR-340 AND SER-347, MASS SPECTROMETRY. Tissue: Melanoma.
[3]	"Stereochemical assignment, antiinflammatory properties, and receptor for the omega-3 lipid mediator resolvin E1." Arita M., Bianchini F., Aliberti J., Sher A., Chiang N., Hong S., Yang R., Petasis N.A., Serhan C.N. J. Exp. Med. 201:713-722(2005) [PubMed] [Europe PMC] [Abstract] Cited for: LIGAND-BINDING, FUNCTION.
[4]	"Chemokine-like receptor 1 expression by macrophages in vivo: regulation by TGF-beta and TLR ligands." Zabel B.A., Ohyama T., Zuniga L., Kim J.Y., Johnston B., Allen S.J., Guido D.G., Handel T.M., Butcher E.C. Exp. Hematol. 34:1106-1114(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model." Luangsay S., Wittamer V., Bondue B., De Henau O., Rouger L., Brait M., Franssen J.D., de Nadai P., Huaux F., Parmentier M. J. Immunol. 183:6489-6499(2009) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U79525 mRNA. Translation: AAB53789.1.
IPI	IPI00125549.
PIR	JC5498.
RefSeq	NP_032179.1. NM_008153.3.
UniGene	Mm.5196.
3D structure databases
ProteinModelPortal	P97468.
SMR	P97468. Positions 36-322.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P97468.
Proteomic databases
PRIDE	P97468.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000047936; ENSMUSP00000036316; ENSMUSG00000042190.
GeneID	14747.
KEGG	mmu:14747.
Organism-specific databases
CTD	1240.
MGI	MGI:109603. Cmklr1.
Phylogenomic databases
eggNOG	NOG150376.
HOGENOM	HOG000234122.
HOVERGEN	HBG107927.
InParanoid	P97468.
KO	K04245.
OMA	ACYLTIV.
OrthoDB	EOG4SXNCW.
Gene expression databases
ArrayExpress	P97468.
Bgee	P97468.
CleanEx	MM_CMKLR1.
Genevestigator	P97468.
GermOnline	ENSMUSG00000042190. Mus musculus.
Family and domain databases
InterPro	IPR002258. DEZorph_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01126. DEZORPHANR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CMKLR1. mouse.
NextBio	286807.
SOURCE	Search...

Entry information

Entry name

CML1_MOUSE

Accession

Primary (citable) accession number: P97468

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents