Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P97468

- CML1_MOUSE

UniProt

P97468 - CML1_MOUSE

Protein

Chemokine-like receptor 1

Gene

Cmklr1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B By similarity. Positively regulates adipogenesis and adipocyte metabolism.By similarity3 Publications

    GO - Molecular functioni

    1. chemokine receptor activity Source: InterPro

    GO - Biological processi

    1. chemotaxis Source: InterPro
    2. negative regulation of interleukin-12 production Source: UniProtKB
    3. negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
    4. positive regulation of fat cell differentiation Source: UniProtKB
    5. positive regulation of macrophage chemotaxis Source: Ensembl
    6. regulation of calcium-mediated signaling Source: Ensembl

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chemokine-like receptor 1
    Alternative name(s):
    G-protein coupled receptor DEZ
    Gene namesi
    Name:Cmklr1
    Synonyms:Dez, Gpcr27
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:109603. Cmklr1.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 371371Chemokine-like receptor 1PRO_0000069308Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi7 – 71N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi110 ↔ 187PROSITE-ProRule annotation
    Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP97468.

    PTM databases

    PhosphoSiteiP97468.

    Expressioni

    Tissue specificityi

    Expressed in the differentiated adipocytes (at protein level). Ubiquitous. Highly expressed in adipose tissue and immature plasmacytoid dendritic cells (DCs) and at lower levels in myeloid DCs, macrophages, and NK cells. Expressed on macrophages isolated from different tissues, including peritoneal cavities, pleural cavities and spleen.5 Publications

    Developmental stagei

    Expressed during bone development and in adult parathyroid glands. Expressed at day E11 in the caudal part of the tongue and the umbilical cord and the expression in the tongue was maintained throughout adulthood. Expression increases in bone and cartilaginous forming zones of embryo up to stage E14.5 and at E16.5 expression is seen in the lung.1 Publication

    Inductioni

    Down-regulated by bacterial lipopolysaccharide (LPS), IFN-alpha and TNF on macrophages.1 Publication

    Gene expression databases

    ArrayExpressiP97468.
    BgeeiP97468.
    CleanExiMM_CMKLR1.
    GenevestigatoriP97468.

    Structurei

    3D structure databases

    ProteinModelPortaliP97468.
    SMRiP97468. Positions 36-322.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3939ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini63 – 7311CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini96 – 11217ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini134 – 15219CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini175 – 22248ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini244 – 25916CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini281 – 29818ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini319 – 37153CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei40 – 6223Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei74 – 9522Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei113 – 13321Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei153 – 17422Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei223 – 24321Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei260 – 28021Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei299 – 31820Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG150376.
    HOGENOMiHOG000234122.
    HOVERGENiHBG107927.
    InParanoidiP97468.
    KOiK04245.
    OMAiACYLTIV.
    OrthoDBiEOG7SR4MM.
    PhylomeDBiP97468.
    TreeFamiTF330976.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000355. Chemokine_rcpt.
    IPR002258. DEZorph_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PANTHERiPTHR24227. PTHR24227. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR01126. DEZORPHANR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P97468-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEYDAYNDSG IYDDEYSDGF GYFVDLEEAS PWEAKVAPVF LVVIYSLVCF    50
    LGLLGNGLVI VIATFKMKKT VNTVWFVNLA VADFLFNIFL PMHITYAAMD 100
    YHWVFGKAMC KISNFLLSHN MYTSVFLLTV ISFDRCISVL LPVWSQNHRS 150
    IRLAYMTCSA VWVLAFFLSS PSLVFRDTAN IHGKITCFNN FSLAAPESSP 200
    HPAHSQVVST GYSRHVAVTV TRFLCGFLIP VFIITACYLT IVFKLQRNRL 250
    AKNKKPFKII ITIIITFFLC WCPYHTLYLL ELHHTAVPSS VFSLGLPLAT 300
    AVAIANSCMN PILYVFMGHD FRKFKVALFS RLANALSEDT GPSSYPSHRS 350
    FTKMSSLNEK ASVNEKETST L 371
    Length:371
    Mass (Da):41,815
    Last modified:May 1, 1997 - v1
    Checksum:iCDBE19305244C0B3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U79525 mRNA. Translation: AAB53789.1.
    CCDSiCCDS19551.1.
    PIRiJC5498.
    RefSeqiNP_032179.1. NM_008153.3.
    XP_006530227.1. XM_006530164.1.
    XP_006530228.1. XM_006530165.1.
    XP_006530229.1. XM_006530166.1.
    XP_006530230.1. XM_006530167.1.
    UniGeneiMm.5196.

    Genome annotation databases

    EnsembliENSMUST00000047936; ENSMUSP00000036316; ENSMUSG00000042190.
    GeneIDi14747.
    KEGGimmu:14747.
    UCSCiuc012ebo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U79525 mRNA. Translation: AAB53789.1 .
    CCDSi CCDS19551.1.
    PIRi JC5498.
    RefSeqi NP_032179.1. NM_008153.3.
    XP_006530227.1. XM_006530164.1.
    XP_006530228.1. XM_006530165.1.
    XP_006530229.1. XM_006530166.1.
    XP_006530230.1. XM_006530167.1.
    UniGenei Mm.5196.

    3D structure databases

    ProteinModelPortali P97468.
    SMRi P97468. Positions 36-322.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    GuidetoPHARMACOLOGYi 79.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P97468.

    Proteomic databases

    PRIDEi P97468.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047936 ; ENSMUSP00000036316 ; ENSMUSG00000042190 .
    GeneIDi 14747.
    KEGGi mmu:14747.
    UCSCi uc012ebo.1. mouse.

    Organism-specific databases

    CTDi 1240.
    MGIi MGI:109603. Cmklr1.

    Phylogenomic databases

    eggNOGi NOG150376.
    HOGENOMi HOG000234122.
    HOVERGENi HBG107927.
    InParanoidi P97468.
    KOi K04245.
    OMAi ACYLTIV.
    OrthoDBi EOG7SR4MM.
    PhylomeDBi P97468.
    TreeFami TF330976.

    Miscellaneous databases

    ChiTaRSi CMKLR1. mouse.
    NextBioi 286807.
    PROi P97468.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97468.
    Bgeei P97468.
    CleanExi MM_CMKLR1.
    Genevestigatori P97468.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000355. Chemokine_rcpt.
    IPR002258. DEZorph_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    PANTHERi PTHR24227. PTHR24227. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01126. DEZORPHANR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel G protein-coupled receptor with homology to neuropeptide and chemoattractant receptors expressed during bone development."
      Methner A., Hermey G., Schinke B., Hermans-Borgmeyer I.
      Biochem. Biophys. Res. Commun. 233:336-342(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Tissue: Brain.
    2. "Stereochemical assignment, antiinflammatory properties, and receptor for the omega-3 lipid mediator resolvin E1."
      Arita M., Bianchini F., Aliberti J., Sher A., Chiang N., Hong S., Yang R., Petasis N.A., Serhan C.N.
      J. Exp. Med. 201:713-722(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: LIGAND-BINDING, FUNCTION.
    3. "Chemokine-like receptor 1 expression by macrophages in vivo: regulation by TGF-beta and TLR ligands."
      Zabel B.A., Ohyama T., Zuniga L., Kim J.Y., Johnston B., Allen S.J., Guido D.G., Handel T.M., Butcher E.C.
      Exp. Hematol. 34:1106-1114(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    4. "Chemerin--a new adipokine that modulates adipogenesis via its own receptor."
      Roh S.G., Song S.H., Choi K.C., Katoh K., Wittamer V., Parmentier M., Sasaki S.
      Biochem. Biophys. Res. Commun. 362:1013-1018(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    5. "Chemerin, a novel adipokine that regulates adipogenesis and adipocyte metabolism."
      Goralski K.B., McCarthy T.C., Hanniman E.A., Zabel B.A., Butcher E.C., Parlee S.D., Muruganandan S., Sinal C.J.
      J. Biol. Chem. 282:28175-28188(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    6. "Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes."
      Takahashi M., Takahashi Y., Takahashi K., Zolotaryov F.N., Hong K.S., Kitazawa R., Iida K., Okimura Y., Kaji H., Kitazawa S., Kasuga M., Chihara K.
      FEBS Lett. 582:573-578(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model."
      Luangsay S., Wittamer V., Bondue B., De Henau O., Rouger L., Brait M., Franssen J.D., de Nadai P., Huaux F., Parmentier M.
      J. Immunol. 183:6489-6499(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiCML1_MOUSE
    AccessioniPrimary (citable) accession number: P97468
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3