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Protein

Chemokine-like receptor 1

Gene

Cmklr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B (By similarity). Positively regulates adipogenesis and adipocyte metabolism.By similarity3 Publications

GO - Molecular functioni

  1. chemokine receptor activity Source: InterPro

GO - Biological processi

  1. chemotaxis Source: MGI
  2. G-protein coupled receptor signaling pathway Source: MGI
  3. negative regulation of interleukin-12 production Source: UniProtKB
  4. negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
  5. positive regulation of fat cell differentiation Source: UniProtKB
  6. positive regulation of macrophage chemotaxis Source: MGI
  7. regulation of calcium-mediated signaling Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Chemokine-like receptor 1
Alternative name(s):
G-protein coupled receptor DEZ
Gene namesi
Name:Cmklr1
Synonyms:Dez, Gpcr27
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:109603. Cmklr1.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3939ExtracellularSequence AnalysisAdd
BLAST
Transmembranei40 – 6223Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini63 – 7311CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei74 – 9522Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini96 – 11217ExtracellularSequence AnalysisAdd
BLAST
Transmembranei113 – 13321Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini134 – 15219CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei153 – 17422Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini175 – 22248ExtracellularSequence AnalysisAdd
BLAST
Transmembranei223 – 24321Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini244 – 25916CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei260 – 28021Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini281 – 29818ExtracellularSequence AnalysisAdd
BLAST
Transmembranei299 – 31820Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini319 – 37153CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 371371Chemokine-like receptor 1PRO_0000069308Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi7 – 71N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi110 ↔ 187PROSITE-ProRule annotation
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP97468.

PTM databases

PhosphoSiteiP97468.

Expressioni

Tissue specificityi

Expressed in the differentiated adipocytes (at protein level). Ubiquitous. Highly expressed in adipose tissue and immature plasmacytoid dendritic cells (DCs) and at lower levels in myeloid DCs, macrophages, and NK cells. Expressed on macrophages isolated from different tissues, including peritoneal cavities, pleural cavities and spleen.5 Publications

Developmental stagei

Expressed during bone development and in adult parathyroid glands. Expressed at day E11 in the caudal part of the tongue and the umbilical cord and the expression in the tongue was maintained throughout adulthood. Expression increases in bone and cartilaginous forming zones of embryo up to stage E14.5 and at E16.5 expression is seen in the lung.1 Publication

Inductioni

Down-regulated by bacterial lipopolysaccharide (LPS), IFN-alpha and TNF on macrophages.1 Publication

Gene expression databases

BgeeiP97468.
CleanExiMM_CMKLR1.
ExpressionAtlasiP97468. baseline and differential.
GenevestigatoriP97468.

Structurei

3D structure databases

ProteinModelPortaliP97468.
SMRiP97468. Positions 36-322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG150376.
HOGENOMiHOG000234122.
HOVERGENiHBG107927.
InParanoidiP97468.
KOiK04245.
OMAiACYLTIV.
OrthoDBiEOG7SR4MM.
PhylomeDBiP97468.
TreeFamiTF330976.

Family and domain databases

InterProiIPR000355. Chemokine_rcpt.
IPR002258. DEZorph_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24227. PTHR24227. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01126. DEZORPHANR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97468-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYDAYNDSG IYDDEYSDGF GYFVDLEEAS PWEAKVAPVF LVVIYSLVCF
60 70 80 90 100
LGLLGNGLVI VIATFKMKKT VNTVWFVNLA VADFLFNIFL PMHITYAAMD
110 120 130 140 150
YHWVFGKAMC KISNFLLSHN MYTSVFLLTV ISFDRCISVL LPVWSQNHRS
160 170 180 190 200
IRLAYMTCSA VWVLAFFLSS PSLVFRDTAN IHGKITCFNN FSLAAPESSP
210 220 230 240 250
HPAHSQVVST GYSRHVAVTV TRFLCGFLIP VFIITACYLT IVFKLQRNRL
260 270 280 290 300
AKNKKPFKII ITIIITFFLC WCPYHTLYLL ELHHTAVPSS VFSLGLPLAT
310 320 330 340 350
AVAIANSCMN PILYVFMGHD FRKFKVALFS RLANALSEDT GPSSYPSHRS
360 370
FTKMSSLNEK ASVNEKETST L
Length:371
Mass (Da):41,815
Last modified:April 30, 1997 - v1
Checksum:iCDBE19305244C0B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U79525 mRNA. Translation: AAB53789.1.
CCDSiCCDS19551.1.
PIRiJC5498.
RefSeqiNP_032179.1. NM_008153.3.
XP_006530227.1. XM_006530164.2.
XP_006530228.1. XM_006530165.2.
XP_006530229.1. XM_006530166.2.
UniGeneiMm.5196.

Genome annotation databases

EnsembliENSMUST00000047936; ENSMUSP00000036316; ENSMUSG00000042190.
GeneIDi14747.
KEGGimmu:14747.
UCSCiuc012ebo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U79525 mRNA. Translation: AAB53789.1.
CCDSiCCDS19551.1.
PIRiJC5498.
RefSeqiNP_032179.1. NM_008153.3.
XP_006530227.1. XM_006530164.2.
XP_006530228.1. XM_006530165.2.
XP_006530229.1. XM_006530166.2.
UniGeneiMm.5196.

3D structure databases

ProteinModelPortaliP97468.
SMRiP97468. Positions 36-322.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

GuidetoPHARMACOLOGYi79.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP97468.

Proteomic databases

PRIDEiP97468.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047936; ENSMUSP00000036316; ENSMUSG00000042190.
GeneIDi14747.
KEGGimmu:14747.
UCSCiuc012ebo.1. mouse.

Organism-specific databases

CTDi1240.
MGIiMGI:109603. Cmklr1.

Phylogenomic databases

eggNOGiNOG150376.
HOGENOMiHOG000234122.
HOVERGENiHBG107927.
InParanoidiP97468.
KOiK04245.
OMAiACYLTIV.
OrthoDBiEOG7SR4MM.
PhylomeDBiP97468.
TreeFamiTF330976.

Miscellaneous databases

ChiTaRSiCmklr1. mouse.
NextBioi286807.
PROiP97468.
SOURCEiSearch...

Gene expression databases

BgeeiP97468.
CleanExiMM_CMKLR1.
ExpressionAtlasiP97468. baseline and differential.
GenevestigatoriP97468.

Family and domain databases

InterProiIPR000355. Chemokine_rcpt.
IPR002258. DEZorph_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24227. PTHR24227. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01126. DEZORPHANR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A novel G protein-coupled receptor with homology to neuropeptide and chemoattractant receptors expressed during bone development."
    Methner A., Hermey G., Schinke B., Hermans-Borgmeyer I.
    Biochem. Biophys. Res. Commun. 233:336-342(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Tissue: Brain.
  2. "Stereochemical assignment, antiinflammatory properties, and receptor for the omega-3 lipid mediator resolvin E1."
    Arita M., Bianchini F., Aliberti J., Sher A., Chiang N., Hong S., Yang R., Petasis N.A., Serhan C.N.
    J. Exp. Med. 201:713-722(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: LIGAND-BINDING, FUNCTION.
  3. "Chemokine-like receptor 1 expression by macrophages in vivo: regulation by TGF-beta and TLR ligands."
    Zabel B.A., Ohyama T., Zuniga L., Kim J.Y., Johnston B., Allen S.J., Guido D.G., Handel T.M., Butcher E.C.
    Exp. Hematol. 34:1106-1114(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. "Chemerin--a new adipokine that modulates adipogenesis via its own receptor."
    Roh S.G., Song S.H., Choi K.C., Katoh K., Wittamer V., Parmentier M., Sasaki S.
    Biochem. Biophys. Res. Commun. 362:1013-1018(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Chemerin, a novel adipokine that regulates adipogenesis and adipocyte metabolism."
    Goralski K.B., McCarthy T.C., Hanniman E.A., Zabel B.A., Butcher E.C., Parlee S.D., Muruganandan S., Sinal C.J.
    J. Biol. Chem. 282:28175-28188(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. "Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes."
    Takahashi M., Takahashi Y., Takahashi K., Zolotaryov F.N., Hong K.S., Kitazawa R., Iida K., Okimura Y., Kaji H., Kitazawa S., Kasuga M., Chihara K.
    FEBS Lett. 582:573-578(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model."
    Luangsay S., Wittamer V., Bondue B., De Henau O., Rouger L., Brait M., Franssen J.D., de Nadai P., Huaux F., Parmentier M.
    J. Immunol. 183:6489-6499(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiCML1_MOUSE
AccessioniPrimary (citable) accession number: P97468
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 14, 1998
Last sequence update: April 30, 1997
Last modified: March 31, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.