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UniProtKB - P97465 (DOK1_MOUSE)

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Protein

Docking protein 1

Gene

Dok1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3 (By similarity).By similarity

GO - Molecular functioni

  • insulin receptor binding Source: InterPro
  • signal transducer activity, downstream of receptor Source: MGI
Complete GO annotation...

GO - Biological processi

  • intracellular signal transduction Source: MGI
  • MAPK cascade Source: MGI
  • Ras protein signal transduction Source: MGI
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-8849469. PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1.
R-MMU-8853659. RET signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Docking protein 1
Alternative name(s):
Downstream of tyrosine kinase 1
p62(dok)
Gene namesi
Name:Dok1
Synonyms:Dok
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:893587. Dok1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice appear healthy and are fertile.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872691 – 482Docking protein 1Add BLAST482

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei48PhosphoserineBy similarity1
Modified residuei269PhosphoserineCombined sources1
Modified residuei290PhosphoserineBy similarity1
Modified residuei295PhosphotyrosineCombined sources1
Modified residuei336PhosphotyrosineCombined sources1
Modified residuei340PhosphotyrosineCombined sources1
Modified residuei361PhosphotyrosineCombined sources1
Modified residuei376PhosphotyrosineCombined sources1
Modified residuei397Phosphotyrosine; by INSRBy similarity1
Modified residuei408PhosphotyrosineCombined sources1
Modified residuei415PhosphoserineCombined sources1
Modified residuei450PhosphotyrosineCombined sources1

Post-translational modificationi

Constitutively tyrosine-phosphorylated. Phosphorylated by TEC. Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway (By similarity). Phosphorylated by LYN. Phosphorylated on tyrosine residues by SRMS (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP97465.
MaxQBiP97465.
PaxDbiP97465.
PeptideAtlasiP97465.
PRIDEiP97465.

PTM databases

iPTMnetiP97465.
PhosphoSitePlusiP97465.

Expressioni

Tissue specificityi

Expressed in lung, spleen, skeletal muscle and kidney.

Gene expression databases

BgeeiENSMUSG00000068335.
CleanExiMM_DOK1.
ExpressionAtlasiP97465. baseline and differential.
GenevisibleiP97465. MM.

Interactioni

Subunit structurei

Interacts with RasGAP, INPP5D/SHIP1 and ABL1. Interacts directly with phosphorylated ITGB3 (By similarity). Interacts with SRMS (via the SH2 and SH3 domains) (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Complete GO annotation...

Protein-protein interaction databases

BioGridi199267. 28 interactors.
IntActiP97465. 13 interactors.
MINTiMINT-148034.
STRINGi10090.ENSMUSP00000087079.

Structurei

Secondary structure

1482
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi153 – 160Combined sources8
Helixi163 – 167Combined sources5
Beta strandi172 – 178Combined sources7
Beta strandi180 – 188Combined sources9
Turni190 – 192Combined sources3
Beta strandi194 – 202Combined sources9
Helixi203 – 205Combined sources3
Beta strandi206 – 211Combined sources6
Beta strandi213 – 220Combined sources8
Beta strandi228 – 234Combined sources7
Helixi238 – 253Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P5TX-ray2.35A/B152-266[»]
1UEFX-ray2.50A/B152-266[»]
ProteinModelPortaliP97465.
SMRiP97465.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP97465.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 119PHAdd BLAST116
Domaini151 – 259IRS-type PTBPROSITE-ProRule annotationAdd BLAST109

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi280 – 322Pro-richAdd BLAST43
Compositional biasi359 – 430Pro-richAdd BLAST72

Domaini

PTB domain mediates receptor interaction.

Sequence similaritiesi

Belongs to the DOK family. Type A subfamily.Curated

Phylogenomic databases

eggNOGiKOG4047. Eukaryota.
ENOG410XS2S. LUCA.
GeneTreeiENSGT00730000110348.
HOGENOMiHOG000112245.
HOVERGENiHBG018962.
InParanoidiP97465.
KOiK14752.
OMAiDPKEDPI.
OrthoDBiEOG091G075T.
PhylomeDBiP97465.
TreeFamiTF324994.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiView protein in InterPro
IPR002404. IRS_PTB.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
PfamiView protein in Pfam
PF02174. IRS. 1 hit.
PF00169. PH. 1 hit.
SMARTiView protein in SMART
SM00233. PH. 1 hit.
SM00310. PTBI. 1 hit.
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiView protein in PROSITE
PS51064. IRS_PTB. 1 hit.

Sequencei

Sequence statusi: Complete.

P97465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGAVMEGPL FLQSQRFGTK RWRKTWAVLY PASPHGVARL EFFDHKGSSS 
        60         70         80         90        100
RGGRGGSRRL DCKMIRLAEC VSVVPVTVES PPEPGAVAFR LDTAQRSHLL 
       110        120        130        140        150
AADAVSSTAW VQTLCRTAFP KGGWALAQTE NQPKFSALEM LENSLYSPTW 
       160        170        180        190        200
EGSQFWVTSQ KTEASERCGL QGSYILRVEA EKLTLLTLGA QSQILEPLLF 
       210        220        230        240        250
WPYTLLRRYG RDKVMFSFEA GRRCPSGPGT FTFQTSQGND IFQAVEAAIQ 
       260        270        280        290        300
QQKAQGKVGQ AQDILRTDSH DGETEGKTVP PPVPQDPLGS PPALYAEPLD 
       310        320        330        340        350
SLRIPPGPSQ DSVYSDPLGS TPAGAGEGVH SKKPLYWDLY GHVQQQLLKT 
       360        370        380        390        400
KLTDSKEDPI YDEPEGLAPA PPRGLYDLPQ EPRDAWWCQA RLKEEGYELP 
       410        420        430        440        450
YNPATDDYAV PPPRSPKPAP APKPQGLILP ESGTTRGSGS KGFSSDTALY 
       460        470        480 
SQVQKSGTSG AWDCGLSKVG NDRAGVKSEG ST
Length:482
Mass (Da):52,452
Last modified:January 17, 2003 - v2
Checksum:iC999C9FE0DA58EA3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2D → N in AAH13066 (PubMed:15489334).Curated1
Sequence conflicti87V → A in AAC95339 (PubMed:9927484).Curated1
Sequence conflicti87V → A in AAH13066 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78818 mRNA. Translation: AAB48827.2.
AF084363 Genomic DNA. Translation: AAC95339.1.
BC013066 mRNA. Translation: AAH13066.1.
CCDSiCCDS20265.1.
RefSeqiNP_001278728.1. NM_001291799.1.
NP_034200.4. NM_010070.4.
UniGeneiMm.156.

Genome annotation databases

EnsembliENSMUST00000089651; ENSMUSP00000087079; ENSMUSG00000068335.
GeneIDi13448.
KEGGimmu:13448.
UCSCiuc009clr.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDOK1_MOUSE
AccessioniPrimary (citable) accession number: P97465
Secondary accession number(s): Q9R213
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: January 17, 2003
Last modified: June 7, 2017
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families