ID PROP1_MOUSE Reviewed; 223 AA. AC P97458; Q5NCG9; DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-1997, sequence version 1. DT 24-JAN-2024, entry version 167. DE RecName: Full=Homeobox protein prophet of Pit-1; DE Short=PROP-1; DE AltName: Full=Pituitary-specific homeodomain factor; GN Name=Prop1; Synonyms=Prop-1; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=8934515; DOI=10.1038/384327a0; RA Sornson M.W., Wu W., Dasen J.S., Flynn S.E., Norman D.J., O'Connell S.M., RA Gukovsky I., Carriere C., Ryan A.K., Miller A.P., Zuo L., Gleiberman A.S., RA Andersen B., Beamer W.G., Rosenfeld M.G.; RT "Pituitary lineage determination by the Prophet of Pit-1 homeodomain factor RT defective in Ames dwarfism."; RL Nature 384:327-333(1996). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J; RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112; RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., RA Eichler E.E., Ponting C.P.; RT "Lineage-specific biology revealed by a finished genome assembly of the RT mouse."; RL PLoS Biol. 7:E1000112-E1000112(2009). RN [3] RP MUTAGENESIS OF SER-83 AND PHE-85. RX PubMed=10946881; DOI=10.1210/jcem.85.8.6744; RA Osorio M.G., Kopp P., Marui S., Latronico A.C., Mendonca B.B., RA Arnhold I.J.; RT "Combined pituitary hormone deficiency caused by a novel mutation of a RT highly conserved residue (F88S) in the homeodomain of PROP-1."; RL J. Clin. Endocrinol. Metab. 85:2779-2785(2000). CC -!- FUNCTION: Possibly involved in the ontogenesis of pituitary CC gonadotropes, as well as somatotropes, lactotropes and caudomedial CC thyrotropes. CC -!- INTERACTION: CC P97458; Q02248: Ctnnb1; NbExp=2; IntAct=EBI-937831, EBI-397872; CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}. CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U77946; AAB38884.1; -; mRNA. DR EMBL; AL627215; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR CCDS; CCDS24643.1; -. DR RefSeq; NP_032962.1; NM_008936.1. DR AlphaFoldDB; P97458; -. DR SMR; P97458; -. DR BioGRID; 202394; 4. DR IntAct; P97458; 1. DR STRING; 10090.ENSMUSP00000057231; -. DR PhosphoSitePlus; P97458; -. DR jPOST; P97458; -. DR PaxDb; 10090-ENSMUSP00000057231; -. DR Antibodypedia; 29400; 129 antibodies from 25 providers. DR DNASU; 19127; -. DR Ensembl; ENSMUST00000051159.3; ENSMUSP00000057231.2; ENSMUSG00000044542.4. DR GeneID; 19127; -. DR KEGG; mmu:19127; -. DR UCSC; uc007itg.1; mouse. DR AGR; MGI:109330; -. DR CTD; 5626; -. DR MGI; MGI:109330; Prop1. DR VEuPathDB; HostDB:ENSMUSG00000044542; -. DR eggNOG; KOG0490; Eukaryota. DR GeneTree; ENSGT00940000162292; -. DR HOGENOM; CLU_044912_1_0_1; -. DR InParanoid; P97458; -. DR OMA; CPYSYPT; -. DR OrthoDB; 3673628at2759; -. DR PhylomeDB; P97458; -. DR TreeFam; TF315976; -. DR BioGRID-ORCS; 19127; 2 hits in 77 CRISPR screens. DR PRO; PR:P97458; -. DR Proteomes; UP000000589; Chromosome 11. DR RNAct; P97458; Protein. DR Bgee; ENSMUSG00000044542; Expressed in adenohypophysis and 12 other cell types or tissues. DR ExpressionAtlas; P97458; baseline and differential. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI. DR GO; GO:0008013; F:beta-catenin binding; IPI:MGI. DR GO; GO:0003682; F:chromatin binding; IDA:MGI. DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB. DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB. DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB. DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI. DR GO; GO:0021984; P:adenohypophysis development; IMP:MGI. DR GO; GO:0009887; P:animal organ morphogenesis; IMP:MGI. DR GO; GO:0006915; P:apoptotic process; IMP:MGI. DR GO; GO:0001568; P:blood vessel development; IMP:MGI. DR GO; GO:0016477; P:cell migration; IGI:MGI. DR GO; GO:0009953; P:dorsal/ventral pattern formation; IMP:MGI. DR GO; GO:0048732; P:gland development; IMP:MGI. DR GO; GO:0048850; P:hypophysis morphogenesis; IMP:MGI. DR GO; GO:0021979; P:hypothalamus cell differentiation; IMP:MGI. DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:MGI. DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB. DR GO; GO:0021983; P:pituitary gland development; IMP:MGI. DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI. DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central. DR GO; GO:0060126; P:somatotropin secreting cell differentiation; IMP:MGI. DR CDD; cd00086; homeodomain; 1. DR Gene3D; 1.10.10.60; Homeodomain-like; 1. DR InterPro; IPR009057; Homeobox-like_sf. DR InterPro; IPR017970; Homeobox_CS. DR InterPro; IPR001356; Homeobox_dom. DR InterPro; IPR042412; PROP1. DR PANTHER; PTHR47409; HOMEOBOX PROTEIN PROPHET OF PIT-1; 1. DR PANTHER; PTHR47409:SF1; HOMEOBOX PROTEIN PROPHET OF PIT-1; 1. DR Pfam; PF00046; Homeodomain; 1. DR SMART; SM00389; HOX; 1. DR SUPFAM; SSF46689; Homeodomain-like; 1. DR PROSITE; PS00027; HOMEOBOX_1; 1. DR PROSITE; PS50071; HOMEOBOX_2; 1. DR Genevisible; P97458; MM. PE 1: Evidence at protein level; KW DNA-binding; Homeobox; Nucleus; Reference proteome. FT CHAIN 1..223 FT /note="Homeobox protein prophet of Pit-1" FT /id="PRO_0000049272" FT DNA_BIND 66..125 FT /note="Homeobox" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108" FT REGION 1..69 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 168..223 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 1..17 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 199..215 FT /note="Pro residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MUTAGEN 83 FT /note="S->P: Shows very weak DNA binding to a Prop1 FT response element in gel shift assays; transcriptional FT activation of a luciferase reporter gene is reduced to 43% FT compared with that of wild-type Prop1 in transiently FT transfected human embryonic kidney cells." FT /evidence="ECO:0000269|PubMed:10946881" FT MUTAGEN 85 FT /note="F->S: Shows no significant DNA binding to a Prop1 FT response element in gel shift assays; transcriptional FT activation of a luciferase reporter gene is reduced to FT approximately 34% compared with that of wild-type Prop1 in FT transiently transfected human embryonic kidney cells." FT /evidence="ECO:0000269|PubMed:10946881" SQ SEQUENCE 223 AA; 25025 MW; CD0AC4DFDC383679 CRC64; MEAQRRSHQE KQTKGHACGR SLPEPRVASG TLISTVDRSS EAYRRLSGTE LGRPKLCPQR GRPHSRRRHR TTFNPAQLEQ LESAFGRNQY PDIWAREGLA QDTGLSEARI QVWFQNRRAK QRKQERSLLQ PIAHLSTATF SGFLPESSAY PYTYGTPPPP APCFPHPYSH SLPSQPSTAA SLALPPQPED WYPTLHPAPT GHLPCPPPPP MFPLSLETPK SWN //