Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P97458 (PROP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein prophet of Pit-1

Short name=PROP-1
Alternative name(s):
Pituitary-specific homeodomain factor
Gene names
Name:Prop1
Synonyms:Prop-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Possibly involved in the ontogenesis of pituitary gonadotropes, as well as somatotropes, lactotropes and caudomedial thyrotropes.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the paired homeobox family.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadenohypophysis development

Inferred from mutant phenotype PubMed 18996108. Source: MGI

blood vessel development

Inferred from mutant phenotype PubMed 16556738. Source: MGI

canonical Wnt signaling pathway

Inferred from genetic interaction PubMed 16678101. Source: MGI

cell migration

Inferred from genetic interaction PubMed 18996108. Source: MGI

dorsal/ventral pattern formation

Inferred from mutant phenotype PubMed 12183375PubMed 15459176. Source: MGI

gland development

Inferred from mutant phenotype PubMed 16556738. Source: MGI

hypophysis morphogenesis

Inferred from mutant phenotype PubMed 16678101. Source: MGI

hypothalamus cell differentiation

Inferred from mutant phenotype PubMed 9067988. Source: MGI

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 16556738. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16678101. Source: MGI

organ morphogenesis

Inferred from mutant phenotype PubMed 12183375PubMed 15459176. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16678101. Source: MGI

somatotropin secreting cell differentiation

Inferred from mutant phenotype PubMed 6194978. Source: MGI

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

transcription factor complex

Inferred from direct assay PubMed 16678101. Source: MGI

   Molecular_functionbeta-catenin binding

Inferred from physical interaction PubMed 16678101. Source: MGI

chromatin binding

Inferred from direct assay PubMed 16678101. Source: MGI

protein C-terminus binding

Inferred from physical interaction PubMed 16678101. Source: MGI

protein binding

Inferred from physical interaction PubMed 16678101. Source: IntAct

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Ctnnb1Q022482EBI-937831,EBI-397872

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 223223Homeobox protein prophet of Pit-1
PRO_0000049272

Regions

DNA binding66 – 12560Homeobox

Experimental info

Mutagenesis831S → P: Shows very weak DNA binding to a Prop1 response element in gel shift assays; transcriptional activation of a luciferase reporter gene is reduced to 43% compared with that of wild-type Prop1 in transiently transfected human embryonic kidney cells. Ref.3
Mutagenesis851F → S: Shows no significant DNA binding to a Prop1 response element in gel shift assays; transcriptional activation of a luciferase reporter gene is reduced to approximately 34% compared with that of wild-type Prop1 in transiently transfected human embryonic kidney cells. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P97458 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: CD0AC4DFDC383679

FASTA22325,025
        10         20         30         40         50         60 
MEAQRRSHQE KQTKGHACGR SLPEPRVASG TLISTVDRSS EAYRRLSGTE LGRPKLCPQR 

        70         80         90        100        110        120 
GRPHSRRRHR TTFNPAQLEQ LESAFGRNQY PDIWAREGLA QDTGLSEARI QVWFQNRRAK 

       130        140        150        160        170        180 
QRKQERSLLQ PIAHLSTATF SGFLPESSAY PYTYGTPPPP APCFPHPYSH SLPSQPSTAA 

       190        200        210        220 
SLALPPQPED WYPTLHPAPT GHLPCPPPPP MFPLSLETPK SWN 

« Hide

References

« Hide 'large scale' references
[1]"Pituitary lineage determination by the Prophet of Pit-1 homeodomain factor defective in Ames dwarfism."
Sornson M.W., Wu W., Dasen J.S., Flynn S.E., Norman D.J., O'Connell S.M., Gukovsky I., Carriere C., Ryan A.K., Miller A.P., Zuo L., Gleiberman A.S., Andersen B., Beamer W.G., Rosenfeld M.G.
Nature 384:327-333(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Combined pituitary hormone deficiency caused by a novel mutation of a highly conserved residue (F88S) in the homeodomain of PROP-1."
Osorio M.G., Kopp P., Marui S., Latronico A.C., Mendonca B.B., Arnhold I.J.
J. Clin. Endocrinol. Metab. 85:2779-2785(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF SER-83 AND PHE-85.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U77946 mRNA. Translation: AAB38884.1.
AL627215 Genomic DNA. Translation: CAI35303.1.
CCDSCCDS24643.1.
RefSeqNP_032962.1. NM_008936.1.
UniGeneMm.1386.

3D structure databases

ProteinModelPortalP97458.
SMRP97458. Positions 68-125.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202394. 4 interactions.
IntActP97458. 1 interaction.

PTM databases

PhosphoSiteP97458.

Proteomic databases

PRIDEP97458.

Protocols and materials databases

DNASU19127.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000051159; ENSMUSP00000057231; ENSMUSG00000044542.
GeneID19127.
KEGGmmu:19127.
UCSCuc007itg.1. mouse.

Organism-specific databases

CTD5626.
MGIMGI:109330. Prop1.

Phylogenomic databases

eggNOGNOG293463.
GeneTreeENSGT00750000117223.
HOVERGENHBG053691.
InParanoidQ5NCG9.
KOK09327.
OMAHQSEDWY.
OrthoDBEOG7P8P9G.
PhylomeDBP97458.
TreeFamTF315976.

Gene expression databases

BgeeP97458.
CleanExMM_PROP1.
GenevestigatorP97458.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295738.
PROP97458.
SOURCESearch...

Entry information

Entry namePROP1_MOUSE
AccessionPrimary (citable) accession number: P97458
Secondary accession number(s): Q5NCG9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot