Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homeobox protein prophet of Pit-1

Gene

Prop1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possibly involved in the ontogenesis of pituitary gonadotropes, as well as somatotropes, lactotropes and caudomedial thyrotropes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi66 – 12560HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. beta-catenin binding Source: MGI
  2. chromatin binding Source: MGI
  3. protein C-terminus binding Source: MGI
  4. RNA polymerase II distal enhancer sequence-specific DNA binding Source: NTNU_SB
  5. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
  6. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB

GO - Biological processi

  1. adenohypophysis development Source: MGI
  2. blood vessel development Source: MGI
  3. canonical Wnt signaling pathway Source: MGI
  4. cell migration Source: MGI
  5. dorsal/ventral pattern formation Source: MGI
  6. gland development Source: MGI
  7. hypophysis morphogenesis Source: MGI
  8. hypothalamus cell differentiation Source: MGI
  9. negative regulation of apoptotic process Source: MGI
  10. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  11. organ morphogenesis Source: MGI
  12. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  13. somatotropin secreting cell differentiation Source: MGI
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein prophet of Pit-1
Short name:
PROP-1
Alternative name(s):
Pituitary-specific homeodomain factor
Gene namesi
Name:Prop1
Synonyms:Prop-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:109330. Prop1.

Subcellular locationi

Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi83 – 831S → P: Shows very weak DNA binding to a Prop1 response element in gel shift assays; transcriptional activation of a luciferase reporter gene is reduced to 43% compared with that of wild-type Prop1 in transiently transfected human embryonic kidney cells. 1 Publication
Mutagenesisi85 – 851F → S: Shows no significant DNA binding to a Prop1 response element in gel shift assays; transcriptional activation of a luciferase reporter gene is reduced to approximately 34% compared with that of wild-type Prop1 in transiently transfected human embryonic kidney cells. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Homeobox protein prophet of Pit-1PRO_0000049272Add
BLAST

Proteomic databases

PRIDEiP97458.

PTM databases

PhosphoSiteiP97458.

Expressioni

Gene expression databases

BgeeiP97458.
CleanExiMM_PROP1.
ExpressionAtlasiP97458. baseline.
GenevestigatoriP97458.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Ctnnb1Q022482EBI-937831,EBI-397872

Protein-protein interaction databases

BioGridi202394. 4 interactions.
IntActiP97458. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP97458.
SMRiP97458. Positions 68-125.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the paired homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG293463.
GeneTreeiENSGT00760000118908.
HOVERGENiHBG053691.
InParanoidiP97458.
KOiK09327.
OMAiTCFPHPY.
OrthoDBiEOG7P8P9G.
PhylomeDBiP97458.
TreeFamiTF315976.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAQRRSHQE KQTKGHACGR SLPEPRVASG TLISTVDRSS EAYRRLSGTE
60 70 80 90 100
LGRPKLCPQR GRPHSRRRHR TTFNPAQLEQ LESAFGRNQY PDIWAREGLA
110 120 130 140 150
QDTGLSEARI QVWFQNRRAK QRKQERSLLQ PIAHLSTATF SGFLPESSAY
160 170 180 190 200
PYTYGTPPPP APCFPHPYSH SLPSQPSTAA SLALPPQPED WYPTLHPAPT
210 220
GHLPCPPPPP MFPLSLETPK SWN
Length:223
Mass (Da):25,025
Last modified:April 30, 1997 - v1
Checksum:iCD0AC4DFDC383679
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77946 mRNA. Translation: AAB38884.1.
AL627215 Genomic DNA. Translation: CAI35303.1.
CCDSiCCDS24643.1.
RefSeqiNP_032962.1. NM_008936.1.
UniGeneiMm.1386.

Genome annotation databases

EnsembliENSMUST00000051159; ENSMUSP00000057231; ENSMUSG00000044542.
GeneIDi19127.
KEGGimmu:19127.
UCSCiuc007itg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77946 mRNA. Translation: AAB38884.1.
AL627215 Genomic DNA. Translation: CAI35303.1.
CCDSiCCDS24643.1.
RefSeqiNP_032962.1. NM_008936.1.
UniGeneiMm.1386.

3D structure databases

ProteinModelPortaliP97458.
SMRiP97458. Positions 68-125.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202394. 4 interactions.
IntActiP97458. 1 interaction.

PTM databases

PhosphoSiteiP97458.

Proteomic databases

PRIDEiP97458.

Protocols and materials databases

DNASUi19127.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051159; ENSMUSP00000057231; ENSMUSG00000044542.
GeneIDi19127.
KEGGimmu:19127.
UCSCiuc007itg.1. mouse.

Organism-specific databases

CTDi5626.
MGIiMGI:109330. Prop1.

Phylogenomic databases

eggNOGiNOG293463.
GeneTreeiENSGT00760000118908.
HOVERGENiHBG053691.
InParanoidiP97458.
KOiK09327.
OMAiTCFPHPY.
OrthoDBiEOG7P8P9G.
PhylomeDBiP97458.
TreeFamiTF315976.

Miscellaneous databases

NextBioi295738.
PROiP97458.
SOURCEiSearch...

Gene expression databases

BgeeiP97458.
CleanExiMM_PROP1.
ExpressionAtlasiP97458. baseline.
GenevestigatoriP97458.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Pituitary lineage determination by the Prophet of Pit-1 homeodomain factor defective in Ames dwarfism."
    Sornson M.W., Wu W., Dasen J.S., Flynn S.E., Norman D.J., O'Connell S.M., Gukovsky I., Carriere C., Ryan A.K., Miller A.P., Zuo L., Gleiberman A.S., Andersen B., Beamer W.G., Rosenfeld M.G.
    Nature 384:327-333(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Combined pituitary hormone deficiency caused by a novel mutation of a highly conserved residue (F88S) in the homeodomain of PROP-1."
    Osorio M.G., Kopp P., Marui S., Latronico A.C., Mendonca B.B., Arnhold I.J.
    J. Clin. Endocrinol. Metab. 85:2779-2785(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF SER-83 AND PHE-85.

Entry informationi

Entry nameiPROP1_MOUSE
AccessioniPrimary (citable) accession number: P97458
Secondary accession number(s): Q5NCG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 1998
Last sequence update: April 30, 1997
Last modified: February 3, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.