P97458 (PROP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein prophet of Pit-1
Short name=PROP-1

Alternative name(s):
Pituitary-specific homeodomain factor

Gene names

Name:	Prop1
Synonyms:	Prop-1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possibly involved in the ontogenesis of pituitary gonadotropes, as well as somatotropes, lactotropes and caudomedial thyrotropes.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the paired homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood vessel development Inferred from mutant phenotype PubMed 16556738. Source: MGI canonical Wnt receptor signaling pathway Inferred from genetic interaction PubMed 16678101. Source: MGI cell migration Inferred from genetic interaction PubMed 18996108. Source: MGI dorsal/ventral pattern formation Inferred from mutant phenotype PubMed 12183375PubMed 15459176. Source: MGI hypophysis morphogenesis Inferred from mutant phenotype PubMed 16678101. Source: MGI hypothalamus cell differentiation Inferred from mutant phenotype PubMed 9067988. Source: MGI negative regulation of apoptotic process Inferred from mutant phenotype PubMed 16556738. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 16678101. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 16678101. Source: MGI somatotropin secreting cell differentiation Inferred from mutant phenotype PubMed 6194978. Source: MGI
Cellular_component	transcription factor complex Inferred from direct assay PubMed 16678101. Source: MGI
Molecular_function	chromatin binding Inferred from direct assay PubMed 16678101. Source: MGI sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ctnnb1	Q02248	2	EBI-937831,EBI-397872

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Homeobox protein prophet of Pit-1		PRO_0000049272
Regions
	DNA binding	66 – 125	60	Homeobox
Experimental info
	Mutagenesis	83	1	S → P: Shows very weak DNA binding to a Prop1 response element in gel shift assays; transcriptional activation of a luciferase reporter gene is reduced to 43% compared with that of wild-type Prop1 in transiently transfected human embryonic kidney cells. Ref.3
	Mutagenesis	85	1	F → S: Shows no significant DNA binding to a Prop1 response element in gel shift assays; transcriptional activation of a luciferase reporter gene is reduced to approximately 34% compared with that of wild-type Prop1 in transiently transfected human embryonic kidney cells. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P97458 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: CD0AC4DFDC383679	FASTA	223	25,025
	10 20 30 40 50 60 MEAQRRSHQE KQTKGHACGR SLPEPRVASG TLISTVDRSS EAYRRLSGTE LGRPKLCPQR 70 80 90 100 110 120 GRPHSRRRHR TTFNPAQLEQ LESAFGRNQY PDIWAREGLA QDTGLSEARI QVWFQNRRAK 130 140 150 160 170 180 QRKQERSLLQ PIAHLSTATF SGFLPESSAY PYTYGTPPPP APCFPHPYSH SLPSQPSTAA 190 200 210 220 SLALPPQPED WYPTLHPAPT GHLPCPPPPP MFPLSLETPK SWN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pituitary lineage determination by the Prophet of Pit-1 homeodomain factor defective in Ames dwarfism." Sornson M.W., Wu W., Dasen J.S., Flynn S.E., Norman D.J., O'Connell S.M., Gukovsky I., Carriere C., Ryan A.K., Miller A.P., Zuo L., Gleiberman A.S., Andersen B., Beamer W.G., Rosenfeld M.G. Nature 384:327-333(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Combined pituitary hormone deficiency caused by a novel mutation of a highly conserved residue (F88S) in the homeodomain of PROP-1." Osorio M.G., Kopp P., Marui S., Latronico A.C., Mendonca B.B., Arnhold I.J. J. Clin. Endocrinol. Metab. 85:2779-2785(2000) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF SER-83 AND PHE-85.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U77946 mRNA. Translation: AAB38884.1. AL627215 Genomic DNA. Translation: CAI35303.1.
IPI	IPI00125485.
RefSeq	NP_032962.1. NM_008936.1.
UniGene	Mm.1386.
3D structure databases
ProteinModelPortal	P97458.
SMR	P97458. Positions 68-125.
ModBase	Search...
Protein-protein interaction databases
IntAct	P97458. 1 interaction.
PTM databases
PhosphoSite	P97458.
Proteomic databases
PRIDE	P97458.
Protocols and materials databases
DNASU	19127.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000051159; ENSMUSP00000057231; ENSMUSG00000044542.
GeneID	19127.
KEGG	mmu:19127.
UCSC	uc007itg.1. mouse.
Organism-specific databases
CTD	5626.
MGI	MGI:109330. Prop1.
Phylogenomic databases
eggNOG	NOG293463.
GeneTree	ENSGT00700000104273.
HOVERGEN	HBG053691.
InParanoid	Q5NCG9.
KO	K09327.
OMA	TCFPHPY.
OrthoDB	EOG4BZN3X.
Gene expression databases
ArrayExpress	P97458.
Bgee	P97458.
CleanEx	MM_PROP1.
Genevestigator	P97458.
GermOnline	ENSMUSG00000044542. Mus musculus.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	295738.
SOURCE	Search...

Entry information

Entry name

PROP1_MOUSE

Accession

Primary (citable) accession number: P97458
Secondary accession number(s): Q5NCG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program