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Protein

Myosin regulatory light chain 2, skeletal muscle isoform

Gene

Mylpf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi38 – 49Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • structural constituent of muscle Source: BHF-UCL

GO - Biological processi

  • immune response Source: Ensembl
  • skeletal muscle tissue development Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain 2, skeletal muscle isoform
Alternative name(s):
Fast skeletal myosin light chain 2
MLC2F
Gene namesi
Name:Mylpf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97273. Mylpf.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000193102 – 169Myosin regulatory light chain 2, skeletal muscle isoformAdd BLAST168

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N,N-trimethylalanineBy similarity1
Modified residuei15PhosphoserineCombined sources1
Modified residuei16PhosphoserineCombined sources1
Modified residuei25PhosphothreonineBy similarity1
Modified residuei35PhosphothreonineBy similarity1
Modified residuei75PhosphoserineBy similarity1
Modified residuei101PhosphothreonineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiP97457.
PeptideAtlasiP97457.
PRIDEiP97457.

2D gel databases

SWISS-2DPAGEP97457.
UCD-2DPAGEP97457.

PTM databases

iPTMnetiP97457.
PhosphoSitePlusiP97457.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030672.
ExpressionAtlasiP97457. baseline and differential.
GenevisibleiP97457. MM.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Protein-protein interaction databases

BioGridi201660. 3 interactors.
IntActiP97457. 2 interactors.
MINTiMINT-4102202.
STRINGi10090.ENSMUSP00000032910.

Structurei

3D structure databases

ProteinModelPortaliP97457.
SMRiP97457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 60EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini95 – 130EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini131 – 166EF-hand 3PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0031. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119196.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP97457.
KOiK12758.
OMAiQCDRFSP.
OrthoDBiEOG091G0QF6.
PhylomeDBiP97457.
TreeFamiTF314218.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKKAKRRA GAEGSSNVFS MFDQTQIQEF KEAFTVIDQN RDGIIDKEDL
60 70 80 90 100
RDTFAAMGRL NVKNEELDAM MKEASGPINF TVFLTMFGEK LKGADPEDVI
110 120 130 140 150
TGAFKVLDPE GKGTIKKQFL EELLTTQCDR FSQEEIKNMW AAFPPDVGGN
160
VDYKNICYVI THGDAKDQE
Length:169
Mass (Da):18,955
Last modified:July 5, 2004 - v3
Checksum:i06E4E1126D3A6DB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77943 mRNA. Translation: AAB19118.1.
BC055869 mRNA. Translation: AAH55869.1.
CCDSiCCDS40140.1.
RefSeqiNP_058034.1. NM_016754.5.
UniGeneiMm.14526.

Genome annotation databases

EnsembliENSMUST00000032910; ENSMUSP00000032910; ENSMUSG00000030672.
GeneIDi17907.
KEGGimmu:17907.
UCSCiuc009jup.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77943 mRNA. Translation: AAB19118.1.
BC055869 mRNA. Translation: AAH55869.1.
CCDSiCCDS40140.1.
RefSeqiNP_058034.1. NM_016754.5.
UniGeneiMm.14526.

3D structure databases

ProteinModelPortaliP97457.
SMRiP97457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201660. 3 interactors.
IntActiP97457. 2 interactors.
MINTiMINT-4102202.
STRINGi10090.ENSMUSP00000032910.

PTM databases

iPTMnetiP97457.
PhosphoSitePlusiP97457.

2D gel databases

SWISS-2DPAGEP97457.
UCD-2DPAGEP97457.

Proteomic databases

PaxDbiP97457.
PeptideAtlasiP97457.
PRIDEiP97457.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032910; ENSMUSP00000032910; ENSMUSG00000030672.
GeneIDi17907.
KEGGimmu:17907.
UCSCiuc009jup.1. mouse.

Organism-specific databases

CTDi29895.
MGIiMGI:97273. Mylpf.

Phylogenomic databases

eggNOGiKOG0031. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119196.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP97457.
KOiK12758.
OMAiQCDRFSP.
OrthoDBiEOG091G0QF6.
PhylomeDBiP97457.
TreeFamiTF314218.

Enzyme and pathway databases

ReactomeiR-MMU-445355. Smooth Muscle Contraction.

Miscellaneous databases

ChiTaRSiMylpf. mouse.
PROiP97457.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030672.
ExpressionAtlasiP97457. baseline and differential.
GenevisibleiP97457. MM.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLRS_MOUSE
AccessioniPrimary (citable) accession number: P97457
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This chain binds calcium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.