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Protein

Mothers against decapentaplegic homolog 5

Gene

Smad5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD) (By similarity). Required for angiogenesis.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi65ZincBy similarity1
Metal bindingi110ZincBy similarity1
Metal bindingi122ZincBy similarity1
Metal bindingi127ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: UniProtKB-KW
  • BMP signaling pathway Source: BHF-UCL
  • bone development Source: MGI
  • cardiac muscle contraction Source: MGI
  • cartilage development Source: MGI
  • cellular response to BMP stimulus Source: BHF-UCL
  • cellular response to organic cyclic compound Source: MGI
  • embryonic pattern specification Source: UniProtKB
  • erythrocyte differentiation Source: MGI
  • germ cell development Source: MGI
  • Mullerian duct regression Source: Ensembl
  • osteoblast fate commitment Source: MGI
  • positive regulation of osteoblast differentiation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus Source: BHF-UCL
  • protein phosphorylation Source: MGI
  • SMAD protein signal transduction Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW
  • transforming growth factor beta receptor signaling pathway Source: MGI
  • ureteric bud development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Angiogenesis, Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-201451. Signaling by BMP.

Names & Taxonomyi

Protein namesi
Recommended name:
Mothers against decapentaplegic homolog 5
Short name:
MAD homolog 5
Short name:
Mothers against DPP homolog 5
Alternative name(s):
Dwarfin-C
Short name:
Dwf-C
SMAD family member 5
Short name:
SMAD 5
Short name:
Smad5
Short name:
mSmad5
Gene namesi
Name:Smad5
Synonyms:Madh5, Msmad5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1328787. Smad5.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • protein complex Source: MGI
  • transcription factor complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000908662 – 465Mothers against decapentaplegic homolog 5Add BLAST464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei463PhosphoserinePROSITE-ProRule annotationBy similarity1
Modified residuei465PhosphoserinePROSITE-ProRule annotationBy similarity1

Post-translational modificationi

Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP97454.
PaxDbiP97454.
PeptideAtlasiP97454.
PRIDEiP97454.

PTM databases

iPTMnetiP97454.
PhosphoSitePlusiP97454.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021540.
CleanExiMM_SMAD5.
GenevisibleiP97454. MM.

Interactioni

Subunit structurei

May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2. Interacts (via MH2 domain) with LEMD3 (By similarity). Interacts with WWP1. Interacts with TMEM119 (PubMed:21239498). Interacts with ZNF8 (PubMed:12370310).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201278. 13 interactors.
IntActiP97454. 2 interactors.
MINTiMINT-99279.
STRINGi10090.ENSMUSP00000065798.

Structurei

Secondary structure

1465
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 20Combined sources8
Helixi26 – 42Combined sources17
Helixi48 – 57Combined sources10
Beta strandi59 – 61Combined sources3
Beta strandi67 – 69Combined sources3
Beta strandi72 – 74Combined sources3
Beta strandi76 – 78Combined sources3
Beta strandi81 – 83Combined sources3
Helixi85 – 93Combined sources9
Helixi101 – 103Combined sources3
Beta strandi104 – 106Combined sources3
Beta strandi110 – 112Combined sources3
Helixi114 – 116Combined sources3
Beta strandi119 – 122Combined sources4
Helixi125 – 127Combined sources3
Beta strandi128 – 130Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZKGX-ray3.05A/B1-143[»]
4ZL2X-ray3.06A/B/C/D1-143[»]
4ZL3X-ray3.20A/B/E/F1-143[»]
ProteinModelPortaliP97454.
SMRiP97454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 137MH1PROSITE-ProRule annotationAdd BLAST125
Domaini271 – 465MH2PROSITE-ProRule annotationAdd BLAST195

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi40 – 46Poly-Lys7

Sequence similaritiesi

Belongs to the dwarfin/SMAD family.Curated
Contains 1 MH1 (MAD homology 1) domain.PROSITE-ProRule annotation
Contains 1 MH2 (MAD homology 2) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3701. Eukaryota.
ENOG410XQKU. LUCA.
GeneTreeiENSGT00760000119091.
HOGENOMiHOG000286018.
HOVERGENiHBG053353.
InParanoidiP97454.
KOiK16790.
OMAiDVQPVEY.
OrthoDBiEOG091G082C.
PhylomeDBiP97454.
TreeFamiTF314923.

Family and domain databases

Gene3Di2.60.200.10. 1 hit.
3.90.520.10. 1 hit.
InterProiIPR013790. Dwarfin.
IPR003619. MAD_homology1_Dwarfin-type.
IPR013019. MAD_homology_MH1.
IPR017855. SMAD_dom-like.
IPR001132. SMAD_dom_Dwarfin-type.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR13703. PTHR13703. 1 hit.
PfamiPF03165. MH1. 1 hit.
PF03166. MH2. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
SM00524. DWB. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56366. SSF56366. 1 hit.
PROSITEiPS51075. MH1. 1 hit.
PS51076. MH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSMASLFSF TSPAVKRLLG WKQGDEEEKW AEKAVDALVK KLKKKKGAME
60 70 80 90 100
ELEKALSSPG QPSKCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS
110 120 130 140 150
HHELKPLDIC EFPFGSKQKE VCINPYHYKR VESPVLPPVL VPRHNEFNPQ
160 170 180 190 200
HSLLVQFRNL SHNEPHMPQN ATFPDSFHQP NNAPFPLSPN SPYPPSPASS
210 220 230 240 250
TYPNSPASSG PGSPFQLPAD TPPPAYMPPD DQMAPDNSQP MDTSSNMIPQ
260 270 280 290 300
TMPSISSRDV QPVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT
310 320 330 340 350
DPSNNKSRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD
360 370 380 390 400
SSIFVQSRNC NFHHGFHPTT VCKIPSSCSL KIFNNQEFAQ LLAQSVNHGF
410 420 430 440 450
EAVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV
460
LTQMGSPLNP ISSVS
Length:465
Mass (Da):52,172
Last modified:May 4, 2001 - v2
Checksum:iD66B638DA76BC20B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti196S → P in AAB39737 (PubMed:9076683).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58993 mRNA. Translation: AAB07871.1.
U77638 mRNA. Translation: AAB39737.1.
AF063006 mRNA. Translation: AAC83580.1.
AK082997 mRNA. Translation: BAC38724.1.
BC050001 mRNA. Translation: AAH50001.2.
CCDSiCCDS26565.1.
RefSeqiNP_001157513.1. NM_001164041.1.
NP_001157514.1. NM_001164042.1.
NP_032567.1. NM_008541.3.
XP_006517180.1. XM_006517117.1.
XP_006517181.1. XM_006517118.3.
UniGeneiMm.272920.

Genome annotation databases

EnsembliENSMUST00000069557; ENSMUSP00000065798; ENSMUSG00000021540.
ENSMUST00000109874; ENSMUSP00000105500; ENSMUSG00000021540.
ENSMUST00000109876; ENSMUSP00000105502; ENSMUSG00000021540.
GeneIDi17129.
KEGGimmu:17129.
UCSCiuc007qsw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58993 mRNA. Translation: AAB07871.1.
U77638 mRNA. Translation: AAB39737.1.
AF063006 mRNA. Translation: AAC83580.1.
AK082997 mRNA. Translation: BAC38724.1.
BC050001 mRNA. Translation: AAH50001.2.
CCDSiCCDS26565.1.
RefSeqiNP_001157513.1. NM_001164041.1.
NP_001157514.1. NM_001164042.1.
NP_032567.1. NM_008541.3.
XP_006517180.1. XM_006517117.1.
XP_006517181.1. XM_006517118.3.
UniGeneiMm.272920.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZKGX-ray3.05A/B1-143[»]
4ZL2X-ray3.06A/B/C/D1-143[»]
4ZL3X-ray3.20A/B/E/F1-143[»]
ProteinModelPortaliP97454.
SMRiP97454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201278. 13 interactors.
IntActiP97454. 2 interactors.
MINTiMINT-99279.
STRINGi10090.ENSMUSP00000065798.

PTM databases

iPTMnetiP97454.
PhosphoSitePlusiP97454.

Proteomic databases

MaxQBiP97454.
PaxDbiP97454.
PeptideAtlasiP97454.
PRIDEiP97454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069557; ENSMUSP00000065798; ENSMUSG00000021540.
ENSMUST00000109874; ENSMUSP00000105500; ENSMUSG00000021540.
ENSMUST00000109876; ENSMUSP00000105502; ENSMUSG00000021540.
GeneIDi17129.
KEGGimmu:17129.
UCSCiuc007qsw.2. mouse.

Organism-specific databases

CTDi4090.
MGIiMGI:1328787. Smad5.

Phylogenomic databases

eggNOGiKOG3701. Eukaryota.
ENOG410XQKU. LUCA.
GeneTreeiENSGT00760000119091.
HOGENOMiHOG000286018.
HOVERGENiHBG053353.
InParanoidiP97454.
KOiK16790.
OMAiDVQPVEY.
OrthoDBiEOG091G082C.
PhylomeDBiP97454.
TreeFamiTF314923.

Enzyme and pathway databases

ReactomeiR-MMU-201451. Signaling by BMP.

Miscellaneous databases

PROiP97454.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021540.
CleanExiMM_SMAD5.
GenevisibleiP97454. MM.

Family and domain databases

Gene3Di2.60.200.10. 1 hit.
3.90.520.10. 1 hit.
InterProiIPR013790. Dwarfin.
IPR003619. MAD_homology1_Dwarfin-type.
IPR013019. MAD_homology_MH1.
IPR017855. SMAD_dom-like.
IPR001132. SMAD_dom_Dwarfin-type.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR13703. PTHR13703. 1 hit.
PfamiPF03165. MH1. 1 hit.
PF03166. MH2. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
SM00524. DWB. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56366. SSF56366. 1 hit.
PROSITEiPS51075. MH1. 1 hit.
PS51076. MH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMAD5_MOUSE
AccessioniPrimary (citable) accession number: P97454
Secondary accession number(s): P70341, Q810K0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 4, 2001
Last modified: November 2, 2016
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.