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P97454

- SMAD5_MOUSE

UniProt

P97454 - SMAD5_MOUSE

Protein

Mothers against decapentaplegic homolog 5

Gene

Smad5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 2 (04 May 2001)
      Previous versions | rss
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    Functioni

    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD) By similarity. Required for angiogenesis.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi65 – 651ZincBy similarity
    Metal bindingi110 – 1101ZincBy similarity
    Metal bindingi122 – 1221ZincBy similarity
    Metal bindingi127 – 1271ZincBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: MGI
    3. RNA polymerase II core promoter sequence-specific DNA binding Source: MGI
    4. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. angiogenesis Source: UniProtKB-KW
    2. BMP signaling pathway Source: BHF-UCL
    3. bone development Source: MGI
    4. cardiac muscle contraction Source: MGI
    5. cartilage development Source: MGI
    6. cellular response to BMP stimulus Source: BHF-UCL
    7. cellular response to organic cyclic compound Source: MGI
    8. embryonic pattern specification Source: UniProtKB
    9. erythrocyte differentiation Source: MGI
    10. germ cell development Source: MGI
    11. Mullerian duct regression Source: Ensembl
    12. osteoblast fate commitment Source: MGI
    13. positive regulation of osteoblast differentiation Source: MGI
    14. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    15. positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus Source: BHF-UCL
    16. protein phosphorylation Source: MGI
    17. transcription, DNA-templated Source: UniProtKB-KW
    18. transforming growth factor beta receptor signaling pathway Source: MGI
    19. ureteric bud development Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Angiogenesis, Differentiation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_220505. Signaling by BMP.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mothers against decapentaplegic homolog 5
    Short name:
    MAD homolog 5
    Short name:
    Mothers against DPP homolog 5
    Alternative name(s):
    Dwarfin-C
    Short name:
    Dwf-C
    SMAD family member 5
    Short name:
    SMAD 5
    Short name:
    Smad5
    Short name:
    mSmad5
    Gene namesi
    Name:Smad5
    Synonyms:Madh5, Msmad5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1328787. Smad5.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: UniProtKB
    3. protein complex Source: MGI
    4. transcription factor complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 465464Mothers against decapentaplegic homolog 5PRO_0000090866Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonineBy similarity
    Modified residuei463 – 4631PhosphoserinePROSITE-ProRule annotation
    Modified residuei465 – 4651PhosphoserinePROSITE-ProRule annotation

    Post-translational modificationi

    Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP97454.
    PRIDEiP97454.

    PTM databases

    PhosphoSiteiP97454.

    Expressioni

    Gene expression databases

    BgeeiP97454.
    CleanExiMM_SMAD5.
    GenevestigatoriP97454.

    Interactioni

    Subunit structurei

    May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2. Interacts (via MH2 domain) with LEMD3 By similarity. Interacts with WWP1.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi201278. 13 interactions.
    IntActiP97454. 2 interactions.
    MINTiMINT-99279.

    Structurei

    3D structure databases

    ProteinModelPortaliP97454.
    SMRiP97454. Positions 10-133, 270-453.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 137125MH1PROSITE-ProRule annotationAdd
    BLAST
    Domaini271 – 465195MH2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi40 – 467Poly-Lys

    Sequence similaritiesi

    Belongs to the dwarfin/SMAD family.Curated
    Contains 1 MH1 (MAD homology 1) domain.PROSITE-ProRule annotation
    Contains 1 MH2 (MAD homology 2) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG330956.
    GeneTreeiENSGT00600000084186.
    HOGENOMiHOG000286018.
    HOVERGENiHBG053353.
    InParanoidiP97454.
    KOiK16790.
    OMAiNRVGEAY.
    OrthoDBiEOG7W1540.
    PhylomeDBiP97454.
    TreeFamiTF314923.

    Family and domain databases

    Gene3Di2.60.200.10. 1 hit.
    3.90.520.10. 1 hit.
    InterProiIPR013790. Dwarfin.
    IPR003619. MAD_homology1_Dwarfin-type.
    IPR013019. MAD_homology_MH1.
    IPR017855. SMAD_dom-like.
    IPR001132. SMAD_dom_Dwarfin-type.
    IPR008984. SMAD_FHA_domain.
    [Graphical view]
    PANTHERiPTHR13703. PTHR13703. 1 hit.
    PfamiPF03165. MH1. 1 hit.
    PF03166. MH2. 1 hit.
    [Graphical view]
    SMARTiSM00523. DWA. 1 hit.
    SM00524. DWB. 1 hit.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.
    SSF56366. SSF56366. 1 hit.
    PROSITEiPS51075. MH1. 1 hit.
    PS51076. MH2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97454-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSMASLFSF TSPAVKRLLG WKQGDEEEKW AEKAVDALVK KLKKKKGAME    50
    ELEKALSSPG QPSKCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS 100
    HHELKPLDIC EFPFGSKQKE VCINPYHYKR VESPVLPPVL VPRHNEFNPQ 150
    HSLLVQFRNL SHNEPHMPQN ATFPDSFHQP NNAPFPLSPN SPYPPSPASS 200
    TYPNSPASSG PGSPFQLPAD TPPPAYMPPD DQMAPDNSQP MDTSSNMIPQ 250
    TMPSISSRDV QPVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT 300
    DPSNNKSRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD 350
    SSIFVQSRNC NFHHGFHPTT VCKIPSSCSL KIFNNQEFAQ LLAQSVNHGF 400
    EAVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV 450
    LTQMGSPLNP ISSVS 465
    Length:465
    Mass (Da):52,172
    Last modified:May 4, 2001 - v2
    Checksum:iD66B638DA76BC20B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti196 – 1961S → P in AAB39737. (PubMed:9076683)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U58993 mRNA. Translation: AAB07871.1.
    U77638 mRNA. Translation: AAB39737.1.
    AF063006 mRNA. Translation: AAC83580.1.
    AK082997 mRNA. Translation: BAC38724.1.
    BC050001 mRNA. Translation: AAH50001.2.
    CCDSiCCDS26565.1.
    RefSeqiNP_001157513.1. NM_001164041.1.
    NP_001157514.1. NM_001164042.1.
    NP_032567.1. NM_008541.3.
    XP_006517180.1. XM_006517117.1.
    XP_006517181.1. XM_006517118.1.
    UniGeneiMm.272920.

    Genome annotation databases

    EnsembliENSMUST00000069557; ENSMUSP00000065798; ENSMUSG00000021540.
    ENSMUST00000109874; ENSMUSP00000105500; ENSMUSG00000021540.
    ENSMUST00000109876; ENSMUSP00000105502; ENSMUSG00000021540.
    GeneIDi17129.
    KEGGimmu:17129.
    UCSCiuc007qsw.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U58993 mRNA. Translation: AAB07871.1 .
    U77638 mRNA. Translation: AAB39737.1 .
    AF063006 mRNA. Translation: AAC83580.1 .
    AK082997 mRNA. Translation: BAC38724.1 .
    BC050001 mRNA. Translation: AAH50001.2 .
    CCDSi CCDS26565.1.
    RefSeqi NP_001157513.1. NM_001164041.1.
    NP_001157514.1. NM_001164042.1.
    NP_032567.1. NM_008541.3.
    XP_006517180.1. XM_006517117.1.
    XP_006517181.1. XM_006517118.1.
    UniGenei Mm.272920.

    3D structure databases

    ProteinModelPortali P97454.
    SMRi P97454. Positions 10-133, 270-453.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201278. 13 interactions.
    IntActi P97454. 2 interactions.
    MINTi MINT-99279.

    PTM databases

    PhosphoSitei P97454.

    Proteomic databases

    PaxDbi P97454.
    PRIDEi P97454.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000069557 ; ENSMUSP00000065798 ; ENSMUSG00000021540 .
    ENSMUST00000109874 ; ENSMUSP00000105500 ; ENSMUSG00000021540 .
    ENSMUST00000109876 ; ENSMUSP00000105502 ; ENSMUSG00000021540 .
    GeneIDi 17129.
    KEGGi mmu:17129.
    UCSCi uc007qsw.2. mouse.

    Organism-specific databases

    CTDi 4090.
    MGIi MGI:1328787. Smad5.

    Phylogenomic databases

    eggNOGi NOG330956.
    GeneTreei ENSGT00600000084186.
    HOGENOMi HOG000286018.
    HOVERGENi HBG053353.
    InParanoidi P97454.
    KOi K16790.
    OMAi NRVGEAY.
    OrthoDBi EOG7W1540.
    PhylomeDBi P97454.
    TreeFami TF314923.

    Enzyme and pathway databases

    Reactomei REACT_220505. Signaling by BMP.

    Miscellaneous databases

    NextBioi 291320.
    PROi P97454.
    SOURCEi Search...

    Gene expression databases

    Bgeei P97454.
    CleanExi MM_SMAD5.
    Genevestigatori P97454.

    Family and domain databases

    Gene3Di 2.60.200.10. 1 hit.
    3.90.520.10. 1 hit.
    InterProi IPR013790. Dwarfin.
    IPR003619. MAD_homology1_Dwarfin-type.
    IPR013019. MAD_homology_MH1.
    IPR017855. SMAD_dom-like.
    IPR001132. SMAD_dom_Dwarfin-type.
    IPR008984. SMAD_FHA_domain.
    [Graphical view ]
    PANTHERi PTHR13703. PTHR13703. 1 hit.
    Pfami PF03165. MH1. 1 hit.
    PF03166. MH2. 1 hit.
    [Graphical view ]
    SMARTi SM00523. DWA. 1 hit.
    SM00524. DWB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    SSF56366. SSF56366. 1 hit.
    PROSITEi PS51075. MH1. 1 hit.
    PS51076. MH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth."
      Yingling J.M., Das P., Savage C., Zhang M., Padgett R.W., Wang X.-F.
      Proc. Natl. Acad. Sci. U.S.A. 93:8940-8944(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The C-terminal domain of Mad-like signal transducers is sufficient for biological activity in the Xenopus embryo and transcriptional activation."
      Meersseman G., Verschueren K., Nelles L., Blumenstock C., Kraft H., Wuytens G., Remacle J., Kozak C.A., Tylzanowski P., Niehrs C., Huylebroeck D.
      Mech. Dev. 61:127-140(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Genomic organization and expression of mouse Smad5."
      Chang H., Kraft H., Verschueren K., Wang P., Huylebroeck D., Matzuk M.M.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 129/Sv.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Spinal cord.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    6. "Functions of mammalian Smad genes as revealed by targeted gene disruption in mice."
      Weinstein M., Yang X., Deng C.-X.
      Cytokine Growth Factor Rev. 11:49-58(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    7. "Negative regulation of transforming growth factor-beta (TGF-beta) signaling by WW domain-containing protein 1 (WWP1)."
      Komuro A., Imamura T., Saitoh M., Yoshida Y., Yamori T., Miyazono K., Miyazawa K.
      Oncogene 23:6914-6923(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH WWP1.

    Entry informationi

    Entry nameiSMAD5_MOUSE
    AccessioniPrimary (citable) accession number: P97454
    Secondary accession number(s): P70341, Q810K0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 4, 2001
    Last sequence update: May 4, 2001
    Last modified: October 1, 2014
    This is version 144 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3