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Protein

Ribosome biogenesis protein BOP1

Gene

Bop1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.UniRule annotation3 Publications

GO - Molecular functioni

  • poly(A) RNA binding Source: MGI
  • ribonucleoprotein complex binding Source: MGI

GO - Biological processi

  • cell proliferation Source: UniProtKB
  • cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB
  • regulation of cell cycle Source: UniProtKB
  • ribosomal large subunit biogenesis Source: MGI
  • rRNA processing Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

ReactomeiR-MMU-6791226. Major pathway of rRNA processing in the nucleolus.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome biogenesis protein BOP1UniRule annotation
Alternative name(s):
Block of proliferation 1 proteinUniRule annotation
Gene namesi
Name:Bop1
Synonyms:Kiaa0124
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1334460. Bop1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 732732Ribosome biogenesis protein BOP1PRO_0000050886Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei108 – 1081PhosphotyrosineBy similarity
Modified residuei112 – 1121PhosphoserineCombined sources
Modified residuei113 – 1131PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP97452.
MaxQBiP97452.
PaxDbiP97452.
PRIDEiP97452.

PTM databases

iPTMnetiP97452.
PhosphoSiteiP97452.

Expressioni

Tissue specificityi

Expressed in brain, gut, heart, kidney, liver, lung, muscle, ovary, skin, spleen and testis.

Inductioni

Expression in quiescent fibroblasts is induced by serum stimulation.1 Publication

Gene expression databases

BgeeiP97452.
CleanExiMM_BOP1.
GenevisibleiP97452. MM.

Interactioni

Subunit structurei

Component of the PeBoW complex, composed of BOP1, PES1 and WDR12 (PubMed:15225545, ECO:0000269|PubMed:17353269). The complex is held together by BOP1, which interacts with PES1 via its N-terminal domain and with WDR12 via a high-affinity interaction between the seven-bladed beta-propeller domains of the 2 proteins. The NOP7 complex associates with the 66S pre-ribosome. The PeBoW complex associates with DDX27, BOP1 interacts directly with DDX27 (By similarity).UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi198380. 2 interactions.
IntActiP97452. 1 interaction.
MINTiMINT-4089312.
STRINGi10090.ENSMUSP00000023217.

Structurei

3D structure databases

ProteinModelPortaliP97452.
SMRiP97452. Positions 377-731.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati397 – 43640WD 1Add
BLAST
Repeati438 – 47841WD 2Add
BLAST
Repeati518 – 56043WD 3Add
BLAST
Repeati563 – 60139WD 4Add
BLAST
Repeati604 – 64340WD 5Add
BLAST
Repeati647 – 68640WD 6Add
BLAST
Repeati702 – 73231WD 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni251 – 413163Sufficient for nucleolar localizationAdd
BLAST

Sequence similaritiesi

Belongs to the WD repeat BOP1/ERB1 family.UniRule annotation
Contains 7 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0650. Eukaryota.
ENOG410XRBX. LUCA.
GeneTreeiENSGT00390000018422.
HOGENOMiHOG000029368.
HOVERGENiHBG008113.
InParanoidiP97452.
KOiK14824.
OMAiEDPDFWR.
OrthoDBiEOG7MPRD9.
PhylomeDBiP97452.
TreeFamiTF300437.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
HAMAPiMF_03027. BOP1.
InterProiIPR028598. BOP1/Erb1.
IPR012953. BOP1_N_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08145. BOP1NT. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM01035. BOP1NT. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97452-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGACGKPHM SPASLPGKRR LEPDQELQIQ EPPLLSDPDS SLSDSEESVF
60 70 80 90 100
SGLEDLGSDS SEEDTEGVAG SSGDEDNHRA EETSEELAQA APLCSRTEEA
110 120 130 140 150
GALAQDEYEE DSSDEEDIRN TVGNVPLAWY DEFPHVGYDL DGKRIYKPLR
160 170 180 190 200
TRDELDQFLD KMDDPDFWRT VQDKMTGRDL RLTDEQVALV HRLQRGQFGD
210 220 230 240 250
SGFNPYEPAV DFFSGDIMIH PVTNRPADKR SFIPSLVEKE KVSRMVHAIK
260 270 280 290 300
MGWIKPRRPH DPTPSFYDLW AQEDPNAVLG RHKMHVPAPK LALPGHAESY
310 320 330 340 350
NPPPEYLPTE EERSAWMQQE PVERKLNFLP QKFPSLRTVP AYSRFIQERF
360 370 380 390 400
ERCLDLYLCP RQRKMRVNVD PEDLIPKLPR PRDLQPFPVC QALVYRGHSD
410 420 430 440 450
LVRCLSVSPG GQWLASGSDD GTLKLWEVAT ARCMKTVHVG GVVRSIAWNP
460 470 480 490 500
NPTICLVAAA MDDAVLLLNP ALGDRLLVGS TDQLLEAFTP PEEPALQPAR
510 520 530 540 550
WLEVSEEEHQ RGLRLRICHS KPVTQVTWHG RGDYLAVVLS SQEHTQVLLH
560 570 580 590 600
QVSRRRSQSP FRRSHGQVQC VAFHPSRPFL LVASQRSIRI YHLLRQELTK
610 620 630 640 650
KLMPNCKWVS SMAVHPAGDN IICGSYDSKL VWFDLDLSTK PYKVLRHHKK
660 670 680 690 700
ALRAVAFHPR YPLFASGSDD GSVIVCHGMV YNDLLQNPLL VPVKVLKGHT
710 720 730
LTRDLGVLDV AFHPTQPWVF SSGADGTIRL FS
Length:732
Mass (Da):82,546
Last modified:May 1, 1997 - v1
Checksum:i48939C86071A84A9
GO

Sequence cautioni

The sequence BAC97868.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561L → S in BAC97868 (PubMed:14621295).Curated
Sequence conflicti56 – 561L → S in AAH12693 (PubMed:15489334).Curated
Sequence conflicti234 – 2341P → L in BAC97868 (PubMed:14621295).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77415 mRNA. Translation: AAB19223.1.
AK129058 mRNA. Translation: BAC97868.1. Different initiation.
AK079627 mRNA. Translation: BAC37708.1.
AK167110 mRNA. Translation: BAE39258.1.
BC012693 mRNA. Translation: AAH12693.1.
AF061503 Genomic DNA. Translation: AAC80429.1.
CCDSiCCDS27570.1.
RefSeqiNP_038509.1. NM_013481.1.
UniGeneiMm.4283.

Genome annotation databases

EnsembliENSMUST00000023217; ENSMUSP00000023217; ENSMUSG00000022557.
GeneIDi12181.
KEGGimmu:12181.
UCSCiuc007wkf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77415 mRNA. Translation: AAB19223.1.
AK129058 mRNA. Translation: BAC97868.1. Different initiation.
AK079627 mRNA. Translation: BAC37708.1.
AK167110 mRNA. Translation: BAE39258.1.
BC012693 mRNA. Translation: AAH12693.1.
AF061503 Genomic DNA. Translation: AAC80429.1.
CCDSiCCDS27570.1.
RefSeqiNP_038509.1. NM_013481.1.
UniGeneiMm.4283.

3D structure databases

ProteinModelPortaliP97452.
SMRiP97452. Positions 377-731.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198380. 2 interactions.
IntActiP97452. 1 interaction.
MINTiMINT-4089312.
STRINGi10090.ENSMUSP00000023217.

PTM databases

iPTMnetiP97452.
PhosphoSiteiP97452.

Proteomic databases

EPDiP97452.
MaxQBiP97452.
PaxDbiP97452.
PRIDEiP97452.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023217; ENSMUSP00000023217; ENSMUSG00000022557.
GeneIDi12181.
KEGGimmu:12181.
UCSCiuc007wkf.1. mouse.

Organism-specific databases

CTDi23246.
MGIiMGI:1334460. Bop1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0650. Eukaryota.
ENOG410XRBX. LUCA.
GeneTreeiENSGT00390000018422.
HOGENOMiHOG000029368.
HOVERGENiHBG008113.
InParanoidiP97452.
KOiK14824.
OMAiEDPDFWR.
OrthoDBiEOG7MPRD9.
PhylomeDBiP97452.
TreeFamiTF300437.

Enzyme and pathway databases

ReactomeiR-MMU-6791226. Major pathway of rRNA processing in the nucleolus.

Miscellaneous databases

ChiTaRSiBop1. mouse.
PROiP97452.
SOURCEiSearch...

Gene expression databases

BgeeiP97452.
CleanExiMM_BOP1.
GenevisibleiP97452. MM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
HAMAPiMF_03027. BOP1.
InterProiIPR028598. BOP1/Erb1.
IPR012953. BOP1_N_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08145. BOP1NT. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM01035. BOP1NT. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of growth suppressors from a cDNA expression library."
    Pestov D.G., Grzeszkiewicz T.M., Lau L.F.
    Oncogene 17:3187-3197(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: SWR/J.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryonic tail.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Spinal cord.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Salivary gland.
  5. "Sequence of the bidirectional promoter mouse heat shock transcription factor I and Bop1."
    Zhang Y., Koushik S., Dai R., Mivechi N.F.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-89.
    Strain: 129/SvJ.
    Tissue: Liver.
  6. "Bop1 is a mouse WD40 repeat nucleolar protein involved in 28S and 5. 8S rRNA processing and 60S ribosome biogenesis."
    Strezoska Z., Pestov D.G., Lau L.F.
    Mol. Cell. Biol. 20:5516-5528(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION.
  7. "Evidence of p53-dependent cross-talk between ribosome biogenesis and the cell cycle: effects of nucleolar protein Bop1 on G(1)/S transition."
    Pestov D.G., Strezoska Z., Lau L.F.
    Mol. Cell. Biol. 21:4246-4255(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Functional inactivation of the mouse nucleolar protein Bop1 inhibits multiple steps in pre-rRNA processing and blocks cell cycle progression."
    Strezoska Z., Pestov D.G., Lau L.F.
    J. Biol. Chem. 277:29617-29625(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. "Physical and functional interaction between Pes1 and Bop1 in mammalian ribosome biogenesis."
    Lapik Y.R., Fernandes C.J., Lau L.F., Pestov D.G.
    Mol. Cell 15:17-29(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PES1.
  10. "Interdependence of Pes1, Bop1, and WDR12 controls nucleolar localization and assembly of the PeBoW complex required for maturation of the 60S ribosomal subunit."
    Rohrmoser M., Hoelzel M., Grimm T., Malamoussi A., Harasim T., Orban M., Pfisterer I., Gruber-Eber A., Kremmer E., Eick D.
    Mol. Cell. Biol. 27:3682-3694(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PES1 AND WDR12, SUBCELLULAR LOCATION.
  11. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112 AND SER-113, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  12. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112 AND SER-113, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiBOP1_MOUSE
AccessioniPrimary (citable) accession number: P97452
Secondary accession number(s): Q3TK87, Q6ZQI9, Q91X31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 1, 1997
Last modified: June 8, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.