Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P97447

- FHL1_MOUSE

UniProt

P97447 - FHL1_MOUSE

Protein

Four and a half LIM domains protein 1

Gene

Fhl1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 3 (15 Nov 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP-J and plays a negative regulatory role in the RBP-J-mediated transcription in mammalian systems.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri7 – 3125C4-typeSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. multicellular organismal development Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Four and a half LIM domains protein 1
    Short name:
    FHL-1
    Alternative name(s):
    KyoT
    RBP-associated molecule 14-1
    Short name:
    RAM14-1
    Skeletal muscle LIM-protein 1
    Short name:
    SLIM
    Short name:
    SLIM-1
    Gene namesi
    Name:Fhl1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1298387. Fhl1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 280279Four and a half LIM domains protein 1PRO_0000075736Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei4 – 41N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP97447.
    PaxDbiP97447.
    PRIDEiP97447.

    Expressioni

    Tissue specificityi

    Isoform 1 seems to be most abundant in each tissue and isoform 2 much less abundant. Isoform 1 is highly expressed in skeletal muscle and lung, and to a lesser extent in heart, brain and kidney. Isoform 2 was found in brain, lung kidney and genital organs.1 Publication

    Gene expression databases

    ArrayExpressiP97447.
    BgeeiP97447.
    CleanExiMM_FHL1.
    GenevestigatoriP97447.

    Interactioni

    Protein-protein interaction databases

    BioGridi199668. 4 interactions.
    DIPiDIP-42041N.
    IntActiP97447. 3 interactions.
    MINTiMINT-1326977.

    Structurei

    Secondary structure

    1
    280
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi169 – 1713

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4J2XX-ray2.85B/D168-194[»]
    ProteinModelPortaliP97447.
    SMRiP97447. Positions 37-159, 162-280.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 9253LIM zinc-binding 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini101 – 15353LIM zinc-binding 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini162 – 21251LIM zinc-binding 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini221 – 27656LIM zinc-binding 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 4 LIM zinc-binding domains.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri7 – 3125C4-typeSequence AnalysisAdd
    BLAST

    Keywords - Domaini

    LIM domain, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG314122.
    GeneTreeiENSGT00740000115334.
    HOGENOMiHOG000231075.
    HOVERGENiHBG074526.
    KOiK14365.
    OrthoDBiEOG7P8P7M.

    Family and domain databases

    Gene3Di2.10.110.10. 5 hits.
    InterProiIPR001781. Znf_LIM.
    [Graphical view]
    PfamiPF00412. LIM. 4 hits.
    [Graphical view]
    SMARTiSM00132. LIM. 4 hits.
    [Graphical view]
    PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
    PS50023. LIM_DOMAIN_2. 4 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: P97447-1) [UniParc]FASTAAdd to Basket

    Also known as: KyoT1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSEKFDCHYC RDPLQGKKYV QKDGRHCCLK CFDKFCANTC VDCRKPISAD    50
    AKEVHYKNRY WHDNCFRCAK CLHPLASETF VSKDGKILCN KCATREDSPR 100
    CKGCFKAIVA GDQNVEYKGT VWHKDCFTCS NCKQVIGTGS FFPKGEDFYC 150
    VTCHETKFAK HCVKCNKAIT SGGITYQDQP WHAECFVCVT CSKKLAGQRF 200
    TAVEDQYYCV DCYKNFVAKK CAGCKNPITG FGKGSSVVAY EGQSWHDYCF 250
    HCKKCSVNLA NKRFVFHNEQ VYCPDCAKKL 280
    Length:280
    Mass (Da):31,889
    Last modified:November 15, 2002 - v3
    Checksum:i76C64FB8087BA36D
    GO
    Isoform 2 (identifier: P97447-2) [UniParc]FASTAAdd to Basket

    Also known as: KyoT2

    The sequence of this isoform differs from the canonical sequence as follows:
         168-194: AITSGGITYQDQPWHAECFVCVTCSKK → GLVKAPVWWPMKDNPGTTTASTAKNAP
         195-280: Missing.

    Show »
    Length:194
    Mass (Da):22,011
    Checksum:iDFA2441C2A9673D2
    GO

    Sequence cautioni

    The sequence AAH29024.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti98 – 981S → F in AAC02727. (PubMed:10049693)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei168 – 19427AITSG…TCSKK → GLVKAPVWWPMKDNPGTTTA STAKNAP in isoform 2. CuratedVSP_003120Add
    BLAST
    Alternative sequencei195 – 28086Missing in isoform 2. CuratedVSP_003121Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77039 mRNA. Translation: AAC02727.1.
    U41739 mRNA. Translation: AAC02805.1.
    AF114380 mRNA. Translation: AAD53229.1.
    BC031120 mRNA. Translation: AAH31120.1.
    BC029024 mRNA. Translation: AAH29024.1. Different initiation.
    CCDSiCCDS30148.1. [P97447-1]
    RefSeqiNP_001070830.1. NM_001077362.2.
    NP_001274729.1. NM_001287800.1. [P97447-1]
    NP_034341.2. NM_010211.3. [P97447-1]
    UniGeneiMm.3126.

    Genome annotation databases

    EnsembliENSMUST00000023854; ENSMUSP00000023854; ENSMUSG00000023092. [P97447-1]
    ENSMUST00000116596; ENSMUSP00000112295; ENSMUSG00000023092. [P97447-1]
    GeneIDi14199.
    KEGGimmu:14199.
    UCSCiuc009tgm.1. mouse. [P97447-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77039 mRNA. Translation: AAC02727.1 .
    U41739 mRNA. Translation: AAC02805.1 .
    AF114380 mRNA. Translation: AAD53229.1 .
    BC031120 mRNA. Translation: AAH31120.1 .
    BC029024 mRNA. Translation: AAH29024.1 . Different initiation.
    CCDSi CCDS30148.1. [P97447-1 ]
    RefSeqi NP_001070830.1. NM_001077362.2.
    NP_001274729.1. NM_001287800.1. [P97447-1 ]
    NP_034341.2. NM_010211.3. [P97447-1 ]
    UniGenei Mm.3126.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4J2X X-ray 2.85 B/D 168-194 [» ]
    ProteinModelPortali P97447.
    SMRi P97447. Positions 37-159, 162-280.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199668. 4 interactions.
    DIPi DIP-42041N.
    IntActi P97447. 3 interactions.
    MINTi MINT-1326977.

    Proteomic databases

    MaxQBi P97447.
    PaxDbi P97447.
    PRIDEi P97447.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023854 ; ENSMUSP00000023854 ; ENSMUSG00000023092 . [P97447-1 ]
    ENSMUST00000116596 ; ENSMUSP00000112295 ; ENSMUSG00000023092 . [P97447-1 ]
    GeneIDi 14199.
    KEGGi mmu:14199.
    UCSCi uc009tgm.1. mouse. [P97447-1 ]

    Organism-specific databases

    CTDi 2273.
    MGIi MGI:1298387. Fhl1.

    Phylogenomic databases

    eggNOGi NOG314122.
    GeneTreei ENSGT00740000115334.
    HOGENOMi HOG000231075.
    HOVERGENi HBG074526.
    KOi K14365.
    OrthoDBi EOG7P8P7M.

    Miscellaneous databases

    NextBioi 285426.
    PROi P97447.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97447.
    Bgeei P97447.
    CleanExi MM_FHL1.
    Genevestigatori P97447.

    Family and domain databases

    Gene3Di 2.10.110.10. 5 hits.
    InterProi IPR001781. Znf_LIM.
    [Graphical view ]
    Pfami PF00412. LIM. 4 hits.
    [Graphical view ]
    SMARTi SM00132. LIM. 4 hits.
    [Graphical view ]
    PROSITEi PS00478. LIM_DOMAIN_1. 4 hits.
    PS50023. LIM_DOMAIN_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle."
      Morgan M.J., Madgwick A.J.A.
      Biochem. Biophys. Res. Commun. 255:245-250(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Skeletal muscle.
    2. "LIM protein KyoT2 negatively regulates transcription by association with the RBP-J DNA-binding protein."
      Taniguchi Y., Furukawa T., Tun T., Han H., Honjo T.
      Mol. Cell. Biol. 18:644-654(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, ALTERNATIVE SPLICING.
    3. "Expression patterns of FHL/SLIM family members suggest important functional roles in skeletal muscle and cardiovascular system."
      Chu P.-H., Ruiz-Lozano P., Zhou Q., Cai C., Chen J.
      Mech. Dev. 95:259-265(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Colon.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND LYS-4, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiFHL1_MOUSE
    AccessioniPrimary (citable) accession number: P97447
    Secondary accession number(s): O55181, Q8K318
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: November 15, 2002
    Last modified: October 1, 2014
    This is version 120 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3