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Protein

Four and a half LIM domains protein 1

Gene

Fhl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP-J and plays a negative regulatory role in the RBP-J-mediated transcription in mammalian systems.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 3125C4-typeSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. ion channel binding Source: MGI
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. multicellular organismal development Source: UniProtKB-KW
  3. negative regulation of cell growth Source: MGI
  4. negative regulation of G1/S transition of mitotic cell cycle Source: MGI
  5. negative regulation of G2/M transition of mitotic cell cycle Source: MGI
  6. positive regulation of potassium ion transport Source: MGI
  7. regulation of membrane depolarization Source: MGI
  8. regulation of potassium ion transmembrane transporter activity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Four and a half LIM domains protein 1
Short name:
FHL-1
Alternative name(s):
KyoT
RBP-associated molecule 14-1
Short name:
RAM14-1
Skeletal muscle LIM-protein 1
Short name:
SLIM
Short name:
SLIM-1
Gene namesi
Name:Fhl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1298387. Fhl1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. focal adhesion Source: MGI
  3. nucleus Source: MGI
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 280279Four and a half LIM domains protein 1PRO_0000075736Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei4 – 41N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP97447.
PaxDbiP97447.
PRIDEiP97447.

Expressioni

Tissue specificityi

Isoform 1 seems to be most abundant in each tissue and isoform 2 much less abundant. Isoform 1 is highly expressed in skeletal muscle and lung, and to a lesser extent in heart, brain and kidney. Isoform 2 was found in brain, lung kidney and genital organs.1 Publication

Gene expression databases

BgeeiP97447.
CleanExiMM_FHL1.
ExpressionAtlasiP97447. baseline and differential.
GenevestigatoriP97447.

Interactioni

Protein-protein interaction databases

BioGridi199668. 5 interactions.
DIPiDIP-42041N.
IntActiP97447. 3 interactions.
MINTiMINT-1326977.

Structurei

Secondary structure

1
280
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi169 – 1713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4J2XX-ray2.85B/D168-194[»]
ProteinModelPortaliP97447.
SMRiP97447. Positions 37-159, 162-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 9253LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini101 – 15353LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini162 – 21251LIM zinc-binding 3PROSITE-ProRule annotationAdd
BLAST
Domaini221 – 27656LIM zinc-binding 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 LIM zinc-binding domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 3125C4-typeSequence AnalysisAdd
BLAST

Keywords - Domaini

LIM domain, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG314122.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000231075.
HOVERGENiHBG074526.
InParanoidiP97447.
KOiK14365.
OrthoDBiEOG7P8P7M.

Family and domain databases

Gene3Di2.10.110.10. 5 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 4 hits.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P97447-1) [UniParc]FASTAAdd to basket

Also known as: KyoT1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEKFDCHYC RDPLQGKKYV QKDGRHCCLK CFDKFCANTC VDCRKPISAD
60 70 80 90 100
AKEVHYKNRY WHDNCFRCAK CLHPLASETF VSKDGKILCN KCATREDSPR
110 120 130 140 150
CKGCFKAIVA GDQNVEYKGT VWHKDCFTCS NCKQVIGTGS FFPKGEDFYC
160 170 180 190 200
VTCHETKFAK HCVKCNKAIT SGGITYQDQP WHAECFVCVT CSKKLAGQRF
210 220 230 240 250
TAVEDQYYCV DCYKNFVAKK CAGCKNPITG FGKGSSVVAY EGQSWHDYCF
260 270 280
HCKKCSVNLA NKRFVFHNEQ VYCPDCAKKL
Length:280
Mass (Da):31,889
Last modified:November 15, 2002 - v3
Checksum:i76C64FB8087BA36D
GO
Isoform 2 (identifier: P97447-2) [UniParc]FASTAAdd to basket

Also known as: KyoT2

The sequence of this isoform differs from the canonical sequence as follows:
     168-194: AITSGGITYQDQPWHAECFVCVTCSKK → GLVKAPVWWPMKDNPGTTTASTAKNAP
     195-280: Missing.

Show »
Length:194
Mass (Da):22,011
Checksum:iDFA2441C2A9673D2
GO

Sequence cautioni

The sequence AAH29024.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti98 – 981S → F in AAC02727 (PubMed:10049693).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei168 – 19427AITSG…TCSKK → GLVKAPVWWPMKDNPGTTTA STAKNAP in isoform 2. CuratedVSP_003120Add
BLAST
Alternative sequencei195 – 28086Missing in isoform 2. CuratedVSP_003121Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77039 mRNA. Translation: AAC02727.1.
U41739 mRNA. Translation: AAC02805.1.
AF114380 mRNA. Translation: AAD53229.1.
BC031120 mRNA. Translation: AAH31120.1.
BC029024 mRNA. Translation: AAH29024.1. Different initiation.
CCDSiCCDS30148.1. [P97447-1]
RefSeqiNP_001070830.1. NM_001077362.2.
NP_001274729.1. NM_001287800.1. [P97447-1]
NP_034341.2. NM_010211.3. [P97447-1]
XP_006527871.2. XM_006527808.2.
UniGeneiMm.3126.

Genome annotation databases

EnsembliENSMUST00000023854; ENSMUSP00000023854; ENSMUSG00000023092. [P97447-1]
ENSMUST00000116596; ENSMUSP00000112295; ENSMUSG00000023092. [P97447-1]
GeneIDi14199.
KEGGimmu:14199.
UCSCiuc009tgm.1. mouse. [P97447-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77039 mRNA. Translation: AAC02727.1.
U41739 mRNA. Translation: AAC02805.1.
AF114380 mRNA. Translation: AAD53229.1.
BC031120 mRNA. Translation: AAH31120.1.
BC029024 mRNA. Translation: AAH29024.1. Different initiation.
CCDSiCCDS30148.1. [P97447-1]
RefSeqiNP_001070830.1. NM_001077362.2.
NP_001274729.1. NM_001287800.1. [P97447-1]
NP_034341.2. NM_010211.3. [P97447-1]
XP_006527871.2. XM_006527808.2.
UniGeneiMm.3126.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4J2XX-ray2.85B/D168-194[»]
ProteinModelPortaliP97447.
SMRiP97447. Positions 37-159, 162-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199668. 5 interactions.
DIPiDIP-42041N.
IntActiP97447. 3 interactions.
MINTiMINT-1326977.

Proteomic databases

MaxQBiP97447.
PaxDbiP97447.
PRIDEiP97447.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023854; ENSMUSP00000023854; ENSMUSG00000023092. [P97447-1]
ENSMUST00000116596; ENSMUSP00000112295; ENSMUSG00000023092. [P97447-1]
GeneIDi14199.
KEGGimmu:14199.
UCSCiuc009tgm.1. mouse. [P97447-1]

Organism-specific databases

CTDi2273.
MGIiMGI:1298387. Fhl1.

Phylogenomic databases

eggNOGiNOG314122.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000231075.
HOVERGENiHBG074526.
InParanoidiP97447.
KOiK14365.
OrthoDBiEOG7P8P7M.

Miscellaneous databases

NextBioi285426.
PROiP97447.
SOURCEiSearch...

Gene expression databases

BgeeiP97447.
CleanExiMM_FHL1.
ExpressionAtlasiP97447. baseline and differential.
GenevestigatoriP97447.

Family and domain databases

Gene3Di2.10.110.10. 5 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 4 hits.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle."
    Morgan M.J., Madgwick A.J.A.
    Biochem. Biophys. Res. Commun. 255:245-250(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Skeletal muscle.
  2. "LIM protein KyoT2 negatively regulates transcription by association with the RBP-J DNA-binding protein."
    Taniguchi Y., Furukawa T., Tun T., Han H., Honjo T.
    Mol. Cell. Biol. 18:644-654(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, ALTERNATIVE SPLICING.
  3. "Expression patterns of FHL/SLIM family members suggest important functional roles in skeletal muscle and cardiovascular system."
    Chu P.-H., Ruiz-Lozano P., Zhou Q., Cai C., Chen J.
    Mech. Dev. 95:259-265(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Colon.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND LYS-4, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiFHL1_MOUSE
AccessioniPrimary (citable) accession number: P97447
Secondary accession number(s): O55181, Q8K318
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: April 1, 2015
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.