P97438 (KCNK2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium channel subfamily K member 2

Alternative name(s):
Outward rectifying potassium channel protein TREK-1
TREK-1 K(+) channel subunit
Two pore potassium channel TPKC1

Gene names

Name:

Kcnk2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Outward rectifying potassium channel. Ref.1
Subunit structure	Homodimer Potential.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	High expression in brain and lung. Also detected in kidney, heart and skeletal muscle. Not detected in liver. In the brain, highest expression in olfactory bulb, hippocampus and cerebellum. Ref.1
Miscellaneous	Inhibited by barium. Activated by volatile general anaesthetics such as chloroform, diethyl ether, halothane and isoflurane.
Sequence similarities	Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Inferred from direct assay PubMed 11560940. Source: MGI stabilization of membrane potential Traceable author statement PubMed 11560940. Source: MGI
Cellular_component	voltage-gated potassium channel complex Inferred from direct assay PubMed 11560940. Source: MGI
Molecular_function	voltage-gated potassium channel activity Inferred from direct assay PubMed 11560940. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Potassium channel subfamily K member 2		PRO_0000101743
Regions
	Topological domain	1 – 46	46	Cytoplasmic Potential
	Transmembrane	47 – 67	21	Helical; Potential
	Intramembrane	129 – 155	27	Pore-forming; Name=Pore-forming 1; Potential
	Transmembrane	157 – 177	21	Helical; Potential
	Topological domain	178 – 207	30	Cytoplasmic Potential
	Transmembrane	208 – 228	21	Helical; Potential
	Intramembrane	238 – 268	31	Pore-forming; Name=Pore-forming 2; Potential
	Transmembrane	273 – 293	21	Helical; Potential
	Topological domain	294 – 411	118	Cytoplasmic Potential
	Region	354 – 411	58	Required for basal channel activity
	Region	378 – 411	34	Essential for chloroform and halothane sensitivity
Amino acid modifications
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P97438 [UniParc]. Last modified November 1, 1999. Version 2. Checksum: 8F976DDD103EFA05	FASTA	411	45,298
	10 20 30 40 50 60 MAAPDLLDPK SAAQNSKPRL SFSSKPTVLA SRVESDSAIN VMKWKTVSTI FLVVVLYLII 70 80 90 100 110 120 GAAVFKALEQ PQEISQRTTI VIQKQTFIAQ HACVNSTELD ELIQQIVAAI NAGIIPLGNS 130 140 150 160 170 180 SNQVSHWDLG SSFFFAGTVI TTIGFGNISP RTEGGKIFCI IYALLGIPLF GFLLAGVGDQ 190 200 210 220 230 240 LGTIFGKGIA KVEDTFIKWN VSQTKIRIIS TIIFILFGCV LFVALPAVIF KHIEGWSALD 250 260 270 280 290 300 AIYFVVITLT TIGFGDYVAG GSDIEYLDFY KPVVWFWILV GLAYFAAVLS MIGDWLRVIS 310 320 330 340 350 360 KKTKEEVGEF RAHAAEWTAN VTAEFKETRR RLSVEIYDKF QRATSVKRKL SAELAGNHNQ 370 380 390 400 410 ELTPCRRTLS VNHLTSEREV LPPLLKAESI YLNGLTPHCA GEDIAVIENM K

References

[1]	"Cloning, functional expression and brain localization of a novel unconventional outward rectifier K+ channel." Fink M., Duprat F., Lesage F., Reyes R., Romey G., Heurteaux C., Lazdunski M. EMBO J. 15:6854-6862(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Brain.
[2]	Fink M., Duprat F., Lesage F., Reyes R., Romey G., Heurteaux C., Lazdunski M. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Inhalational anesthetics activate two-pore-domain background K+ channels." Patel A.J., Honore E., Lesage F., Fink M., Romey G., Lazdunski M. Nat. Neurosci. 2:422-426(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ACTIVATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U73488 mRNA. Translation: AAC53005.2.
IPI	IPI00875469.
RefSeq	NP_034737.2. NM_010607.2.
UniGene	Mm.33304.
3D structure databases
ProteinModelPortal	P97438.
SMR	P97438. Positions 61-303.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-58579N.
Protein family/group databases
TCDB	1.A.1.9.1. voltage-gated ion channel (VIC) superfamily.
PTM databases
PhosphoSite	P97438.
Proteomic databases
PRIDE	P97438.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	16526.
KEGG	mmu:16526.
Organism-specific databases
CTD	3776.
MGI	MGI:109366. Kcnk2.
Phylogenomic databases
eggNOG	COG1226.
HOGENOM	HOG000013106.
HOVERGEN	HBG052234.
InParanoid	P97438.
KO	K04913.
OrthoDB	EOG4PVNZM.
Gene expression databases
CleanEx	MM_KCNK2.
Genevestigator	P97438.
GermOnline	ENSMUSG00000037624. Mus musculus.
Family and domain databases
InterPro	IPR003280. 2pore_dom_K_chnl. IPR003976. 2pore_dom_K_chnl_TREK. IPR013099. Ion_trans_2. [Graphical view]
Pfam	PF07885. Ion_trans_2. 2 hits. [Graphical view]
PRINTS	PR01333. 2POREKCHANEL. PR01499. TREKCHANNEL.
ProtoNet	Search...
Other
NextBio	289923.
SOURCE	Search...

Entry information

Entry name

KCNK2_MOUSE

Accession

Primary (citable) accession number: P97438

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program